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The map label for this gene is smpB

Identifier: 28210141

GI number: 28210141

Start: 379026

End: 379496

Strand: Direct

Name: smpB

Synonym: CTC00385

Alternate gene names: 28210141

Gene position: 379026-379496 (Clockwise)

Preceding gene: 28210140

Following gene: 28210142

Centisome position: 13.54

GC content: 29.09

Gene sequence:

>471_bases
ATGGTCAAAGGTAGAAGCAATAAAACTCTTGCAGAAAATAGAAAAGCAAGACATGATTACTTTGTAGAAGAAGCTTATGA
AGCAGGAATAGAGTTAGTTGGTACAGAAGTAAAATCTATAAGGGAAGGCAAAGCCAATTTAAAGGATAGTTATGCAGAAA
TAAGAAATGGAGAAGTTTTTCTTAGGAATATGCATGTAAGTCCTTATGAACAGGGTAATATTTTCAATAAAGATCCATTA
AGGGATAGAAAATTATTACTACATAAAATGCAAATTTTAAAGCTAACAGCCTATACTACTCAACAAGGATATACTTTAAT
ACCATTATCATTATATCTTAAAAATGGTAGAGTTAAAGTAAATTTAGCTGTAGCTAAAGGTAAAAAGAACTATGATAAAA
GACAAGATTTAATAGAAAAAGCAGCTAAACGCGATATTGAAAGAGAATTAAAAGATAGAAGTAGGTATTAA

Upstream 100 bases:

>100_bases
CAATTAAATAGTTAAAGCTATAAAGTTCTTTGACTAGGAATATAAAGTATTATATAATTATATAACACAGGGAAACATAA
TATTTGAAGTAGGTGTTGGT

Downstream 100 bases:

>100_bases
TTTACTATTGCTAAATTGTTTAAGTTGTTATATCATATAATATAGACCTTAAAATTAAATATTATCGTCCTGTGCAATAT
AGCATGGGGGCGTAAATGGT

Product: SsrA-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 156; Mature: 156

Protein sequence:

>156_residues
MVKGRSNKTLAENRKARHDYFVEEAYEAGIELVGTEVKSIREGKANLKDSYAEIRNGEVFLRNMHVSPYEQGNIFNKDPL
RDRKLLLHKMQILKLTAYTTQQGYTLIPLSLYLKNGRVKVNLAVAKGKKNYDKRQDLIEKAAKRDIERELKDRSRY

Sequences:

>Translated_156_residues
MVKGRSNKTLAENRKARHDYFVEEAYEAGIELVGTEVKSIREGKANLKDSYAEIRNGEVFLRNMHVSPYEQGNIFNKDPL
RDRKLLLHKMQILKLTAYTTQQGYTLIPLSLYLKNGRVKVNLAVAKGKKNYDKRQDLIEKAAKRDIERELKDRSRY
>Mature_156_residues
MVKGRSNKTLAENRKARHDYFVEEAYEAGIELVGTEVKSIREGKANLKDSYAEIRNGEVFLRNMHVSPYEQGNIFNKDPL
RDRKLLLHKMQILKLTAYTTQQGYTLIPLSLYLKNGRVKVNLAVAKGKKNYDKRQDLIEKAAKRDIERELKDRSRY

Specific function: Binds specifically to the ssrA RNA (tmRNA) and is required for stable association of ssrA with ribosomes

COG id: COG0691

COG function: function code O; tmRNA-binding protein

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the smpB family

Homologues:

Organism=Escherichia coli, GI1788973, Length=160, Percent_Identity=44.375, Blast_Score=135, Evalue=2e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SSRP_CLOTE (Q898Q7)

Other databases:

- EMBL:   AE015927
- RefSeq:   NP_781085.1
- ProteinModelPortal:   Q898Q7
- SMR:   Q898Q7
- GeneID:   1059968
- GenomeReviews:   AE015927_GR
- KEGG:   ctc:CTC00385
- NMPDR:   fig|212717.1.peg.307
- HOGENOM:   HBG293422
- OMA:   EKYEAGI
- PhylomeDB:   Q898Q7
- ProtClustDB:   PRK05422
- BioCyc:   CTET212717:CTC_00385-MONOMER
- GO:   GO:0005737
- GO:   GO:0006412
- HAMAP:   MF_00023
- InterPro:   IPR000037
- InterPro:   IPR020081
- Gene3D:   G3DSA:2.40.280.10
- ProDom:   PD004488
- TIGRFAMs:   TIGR00086

Pfam domain/function: PF01668 SmpB; SSF74982 SmpB

EC number: NA

Molecular weight: Translated: 18173; Mature: 18173

Theoretical pI: Translated: 10.36; Mature: 10.36

Prosite motif: PS01317 SSRP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVKGRSNKTLAENRKARHDYFVEEAYEAGIELVGTEVKSIREGKANLKDSYAEIRNGEVF
CCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHCCCEEE
LRNMHVSPYEQGNIFNKDPLRDRKLLLHKMQILKLTAYTTQQGYTLIPLSLYLKNGRVKV
EEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHEECCCCCEEEEEEEEEECCEEEE
NLAVAKGKKNYDKRQDLIEKAAKRDIERELKDRSRY
EEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MVKGRSNKTLAENRKARHDYFVEEAYEAGIELVGTEVKSIREGKANLKDSYAEIRNGEVF
CCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHCCCEEE
LRNMHVSPYEQGNIFNKDPLRDRKLLLHKMQILKLTAYTTQQGYTLIPLSLYLKNGRVKV
EEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHEECCCCCEEEEEEEEEECCEEEE
NLAVAKGKKNYDKRQDLIEKAAKRDIERELKDRSRY
EEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12552129