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The map label for this gene is prfB [H]

Identifier: 28210088

GI number: 28210088

Start: 313440

End: 314417

Strand: Direct

Name: prfB [H]

Synonym: CTC00328

Alternate gene names: 28210088

Gene position: 313440-314417 (Clockwise)

Preceding gene: 28210087

Following gene: 28210090

Centisome position: 11.2

GC content: 32.72

Gene sequence:

>978_bases
ATGCAAGAACCTGATTTTTGGGAAGATATAAAGAGAGCACAAGAAGTAACACAACAGGAAAAATTAATTAAAGATAGATT
AGAAAAGTTTGACTCTTTAGAGAATAGAATAGAAGATTCTTTAGTTCTTATAGAACTTATGAAAGAAGAAGAGGACATTT
CCTCGGCAGGAGAAGTATTAAAAGAGTTAGTGCTACTTAAGAAGGAAATAGAGAGTTTTAGAATAGAAGTTTTATTATCA
GGTGAATATGATAGAAACAATGCTATAATGACATTACATGCAGGAGTAGGGGGAACAGATGCCCAAGATTGGACTGAAAT
GTTGCTTAGAATGTATACAAGATGGGCAGAGAAGAAAGGTTATAAGGTAGATACTTTAGACTTTCTACCAGGTGATGAGG
CAGGAATAAAAAGTGTTACCTTAAAAATAACTGGAGATTTCGCTTATGGATATTTAAAAGCAGAAAAAGGAGTACACAGA
ATTGTAAGGATATCACCTTATAATGCCAATGGAAAAAGACAAACTTCTTTTGCATCCTTAGAATTGATACCAGAACTTAC
AGAAGATCAAGACATAGAAATAATAGATGATGATTTAAAAGTAGATACTTATAGAGCTAGTGGAGCTGGTGGACAACACG
TAAATAAAACAGAATCTGCAGTAAGAATAACTCATATTCCAACAGGAATAGTTGTACAATGCCAATCAGAACGAAGTCAA
CATTCTAATAGGGATACTGCACTAAAAATGCTTAAAGCTAAACTTGTAGAGTTGAAAGAAGCAGAGCACAAAGAAAAGAT
AGAAGATCTAACTGGTGAATTAAAAGATATGGGATGGGGAAGTCAGATAAGATCTTACGTATTTCATCCATATAACTTAG
TAAAAGATCATAGAACTTCAGCAGAAACAGGTAATTTAACAGCAGTAATGGATGGAGATATTGATTTGTTTATAAATGAA
TATTTAAAAGCTAACTAA

Upstream 100 bases:

>100_bases
AAAGATTTGGAAGAATTAAAAGAAACTTTAACAAAAGAAATAGGGGAGTCTCTTTGACCTAACTAGTTTAAAATTTAAAG
TACAAGAACTTCAAAATAAA

Downstream 100 bases:

>100_bases
CCTTACAATACCCTTAAACTTGAGCTATGTTTAAGGGTATTATATTTATACTCTATATGATTTATTTTTTTTTATAATTA
TAAGACTTATTATTGTAGTT

Product: peptide chain release factor 2

Products: NA

Alternate protein names: RF-2 [H]

Number of amino acids: Translated: 325; Mature: 325

Protein sequence:

>325_residues
MQEPDFWEDIKRAQEVTQQEKLIKDRLEKFDSLENRIEDSLVLIELMKEEEDISSAGEVLKELVLLKKEIESFRIEVLLS
GEYDRNNAIMTLHAGVGGTDAQDWTEMLLRMYTRWAEKKGYKVDTLDFLPGDEAGIKSVTLKITGDFAYGYLKAEKGVHR
IVRISPYNANGKRQTSFASLELIPELTEDQDIEIIDDDLKVDTYRASGAGGQHVNKTESAVRITHIPTGIVVQCQSERSQ
HSNRDTALKMLKAKLVELKEAEHKEKIEDLTGELKDMGWGSQIRSYVFHPYNLVKDHRTSAETGNLTAVMDGDIDLFINE
YLKAN

Sequences:

>Translated_325_residues
MQEPDFWEDIKRAQEVTQQEKLIKDRLEKFDSLENRIEDSLVLIELMKEEEDISSAGEVLKELVLLKKEIESFRIEVLLS
GEYDRNNAIMTLHAGVGGTDAQDWTEMLLRMYTRWAEKKGYKVDTLDFLPGDEAGIKSVTLKITGDFAYGYLKAEKGVHR
IVRISPYNANGKRQTSFASLELIPELTEDQDIEIIDDDLKVDTYRASGAGGQHVNKTESAVRITHIPTGIVVQCQSERSQ
HSNRDTALKMLKAKLVELKEAEHKEKIEDLTGELKDMGWGSQIRSYVFHPYNLVKDHRTSAETGNLTAVMDGDIDLFINE
YLKAN
>Mature_325_residues
MQEPDFWEDIKRAQEVTQQEKLIKDRLEKFDSLENRIEDSLVLIELMKEEEDISSAGEVLKELVLLKKEIESFRIEVLLS
GEYDRNNAIMTLHAGVGGTDAQDWTEMLLRMYTRWAEKKGYKVDTLDFLPGDEAGIKSVTLKITGDFAYGYLKAEKGVHR
IVRISPYNANGKRQTSFASLELIPELTEDQDIEIIDDDLKVDTYRASGAGGQHVNKTESAVRITHIPTGIVVQCQSERSQ
HSNRDTALKMLKAKLVELKEAEHKEKIEDLTGELKDMGWGSQIRSYVFHPYNLVKDHRTSAETGNLTAVMDGDIDLFINE
YLKAN

Specific function: Peptide chain release factor 2 directs the termination of translation in response to the peptide chain termination codons UGA and UAA [H]

COG id: COG1186

COG function: function code J; Protein chain release factor B

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI166795303, Length=273, Percent_Identity=38.0952380952381, Blast_Score=180, Evalue=1e-45,
Organism=Homo sapiens, GI34577120, Length=253, Percent_Identity=35.1778656126482, Blast_Score=157, Evalue=2e-38,
Organism=Homo sapiens, GI166795305, Length=187, Percent_Identity=39.0374331550802, Blast_Score=132, Evalue=6e-31,
Organism=Escherichia coli, GI2367172, Length=324, Percent_Identity=47.8395061728395, Blast_Score=318, Evalue=2e-88,
Organism=Escherichia coli, GI1787462, Length=241, Percent_Identity=39.8340248962656, Blast_Score=182, Evalue=3e-47,
Organism=Caenorhabditis elegans, GI17542784, Length=298, Percent_Identity=31.2080536912752, Blast_Score=117, Evalue=7e-27,
Organism=Saccharomyces cerevisiae, GI6321295, Length=236, Percent_Identity=34.7457627118644, Blast_Score=132, Evalue=7e-32,
Organism=Drosophila melanogaster, GI19921226, Length=274, Percent_Identity=36.8613138686131, Blast_Score=156, Evalue=2e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR020853
- InterPro:   IPR004374 [H]

Pfam domain/function: PF03462 PCRF; PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 36997; Mature: 36997

Theoretical pI: Translated: 4.73; Mature: 4.73

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQEPDFWEDIKRAQEVTQQEKLIKDRLEKFDSLENRIEDSLVLIELMKEEEDISSAGEVL
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KELVLLKKEIESFRIEVLLSGEYDRNNAIMTLHAGVGGTDAQDWTEMLLRMYTRWAEKKG
HHHHHHHHHHHHEEEEEEEECCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCC
YKVDTLDFLPGDEAGIKSVTLKITGDFAYGYLKAEKGVHRIVRISPYNANGKRQTSFASL
CEEEEEECCCCCCCCCEEEEEEEECCEEEEEEEHHCCCEEEEEEECCCCCCCCCCHHHHH
ELIPELTEDQDIEIIDDDLKVDTYRASGAGGQHVNKTESAVRITHIPTGIVVQCQSERSQ
HHCCCCCCCCCCEEECCCCEEEEEECCCCCCCCCCCCCCEEEEEECCCCEEEEECCHHHC
HSNRDTALKMLKAKLVELKEAEHKEKIEDLTGELKDMGWGSQIRSYVFHPYNLVKDHRTS
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHCCCC
AETGNLTAVMDGDIDLFINEYLKAN
CCCCCEEEEECCCHHHHHHHHHHCC
>Mature Secondary Structure
MQEPDFWEDIKRAQEVTQQEKLIKDRLEKFDSLENRIEDSLVLIELMKEEEDISSAGEVL
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KELVLLKKEIESFRIEVLLSGEYDRNNAIMTLHAGVGGTDAQDWTEMLLRMYTRWAEKKG
HHHHHHHHHHHHEEEEEEEECCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCC
YKVDTLDFLPGDEAGIKSVTLKITGDFAYGYLKAEKGVHRIVRISPYNANGKRQTSFASL
CEEEEEECCCCCCCCCEEEEEEEECCEEEEEEEHHCCCEEEEEEECCCCCCCCCCHHHHH
ELIPELTEDQDIEIIDDDLKVDTYRASGAGGQHVNKTESAVRITHIPTGIVVQCQSERSQ
HHCCCCCCCCCCEEECCCCEEEEEECCCCCCCCCCCCCCEEEEEECCCCEEEEECCHHHC
HSNRDTALKMLKAKLVELKEAEHKEKIEDLTGELKDMGWGSQIRSYVFHPYNLVKDHRTS
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHCCCC
AETGNLTAVMDGDIDLFINEYLKAN
CCCCCEEEEECCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA