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The map label for this gene is dnaK [H]

Identifier: 27365639

GI number: 27365639

Start: 2342641

End: 2345448

Strand: Reverse

Name: dnaK [H]

Synonym: VV1_2318

Alternate gene names: 27365639

Gene position: 2345448-2342641 (Counterclockwise)

Preceding gene: 27365640

Following gene: 27365638

Centisome position: 71.47

GC content: 51.1

Gene sequence:

>2808_bases
ATGGCATCTCCTCGCTATCTTGTCGGCATTGACTTAGGCACCACCAACACGGTGGTTGCGTTTTGTGAACTGACCGAGAA
CTTACAACAATCCAACGTCTCCCTTTTCGATATTGACCAGCTTATTGGCCCCGGTGAAGTGGTTCGTAAGCCACTACTGC
CCTCTTTCCGCTACCACCCTGCCAACGGGCAAATCGCCCCGGCGGATCTCACCTTACCGTGGGAAAACCAAACTGTGGCT
GGCGACATTGCTAATGTGATCATTGGTGAATGGGCGCGTGAACTGGGCGCAAAGGTAGAAGGGCGTCAGGTTTCAAGCGC
TAAGAGCTGGCTGTCTCACCAAGCGGTTGATCGCCATTCAGACATACTGCCGTGGGCTGGGGTTGGCGATGTTGAGAAGG
TTTCCCCTGTTATTGCCAGTGCCAGTTACCTTAATCACATTCGCCAAGCATGGAATCATCGCCACCCGGGCAATCTGCTC
GAAGATCAAGAGGTGGTGGTCACCGTACCGGCTTCGTTTGATGAAACTGCACGTAAGCTGACCCTCGAAGCCGCTCAACT
GGCAGGCTTAAGCCAAGTGCTTCTGCTCGAAGAGCCTCAAGCGGTTTGTTACGACTGGTATGCTCGCCACCAGCACAGTG
CCGCAGAGTTATTAAAAGAAATCCCGCTGATTTTGGTGTGTGATGTCGGCGGCGGTACCACTGACTTAAGCTTGATTGAA
GCAAAATTTAACCAAGACGCCCTCGCCTTGGATCGCATCGGGGTTGGTGAACACCTCATGCTCGGTGGTGATAACCTAGA
TTTGGCCCTCGCGCATTTAGCGGAGCAGCGCTTTAATCAAAGCAAAAAACTCAATGCGGCCAGCCTCACCAAACTGATTC
AGCAAACACGCAAAGCCAAAGAGCAACTCCTCTCAAGTACAGCGCCGGAAGAAGTGAAGATCACCATGTTGGGCAGTGGT
TCCAAACTGCTTGGTGGCACCAAAAGCATCTCGTTGAATAAACAAGAAGTTCATCAAATCGCCCTCGACGGCTTCTTCCC
ACTTTCGGATTTTACTCAAACGCCGGGTAAACGCCGCAGCGCCGTGGTCGAATTTGGTTTGCCTTATGTCGCCGATCCCG
CGGTGAGCAAACACGTGGCCGAGTTTTTATCTCAGCACCAACAAGTCTCTCACGCTGCGTTAGGACAATCAGAAAGTGAT
CAACCCGCCATTCCGGTAGGTCTGTTGTTGAACGGCGGCGTGTTTAACAGTGACCTCGTCACTGCGCGGATGACCGAGTT
GCTCGGTCGCTGGCGCGGCGCACCGGTCACCGTCCTTGATAACCCGCATCCAGATTGGTCTGTGGCATTAGGCGCTGTCG
CGTTTGCCAAAGCCCGTCGAGGGGCACAACTCAAAATCGGTGGCGGTTCTGCTCGGTCTTACTTTTTGCATCTGCAAGAA
AAAAACAAAATGGGTAAAGCGCTCTGTTTGCTTGCAAAAGGCACCGAAGAAGGACAAGAAATTCGTCTCACTGGACGACG
TTTCTCGCTCACCCTTGGCGAACCCGTACGCTTTAACCTACTCACGTCAACGCACGATGCTTTGTCCCACCACGTAGCGA
TTCAAAACGGCGTCATGGCGGATATCGACCCTGATATCTTCACCCCACTTCCCCCGTATATCGTTACATTGCAAGGGGAA
GGCGCAGAATTAGAAGCCAATCAAAAAGAGCGTGTGGAAGTTCAACTTGCATGCCAATTGACCGAAGTCGGCACCCTGAA
AATGGAGTGCGTCAGTGTCGAAGAAGAAGATAAACGTTGGGCTCTGGAGTTTGAAGTTCGCAACAAAAAAGCGGAAGAAC
AAGAAAATGCCACACTTCACCCACGATTGAATGAGTGTAAAGAACTGATTACCCGCCTCTATAGTGGCAACAAAAAAAGT
GGCGATGGCAATGAGATCAAACTGTTAGCCAAAGAACTTGAACGCAAACTTGGCAAGCGCGATGAATGGGACTTCACAAC
CCTACGCCACTTGTTTGATGCCTTTGCACAAGGACGAAAACGTCGCCGCCGTTCTGAACAACACGAAAAAAACTGGCTAC
GTTTAGCAGGTTTTTCTCTTCGCCCGGGCTTTGGCGATGCAACCGATTCTTGGCGTATTGAGCAAGTTTGGGGGCTCTAC
CAACAAGGTATCCAGTTCCAAAATCATCAAGGTTGGACCGATTGGTGGGTATTCTGGCGTCGTATAGCTGGAGGCTTAAA
CCAAGAGCAACAAGAAACGCTACTTGCCGACATTGCCAAGTATTTACACCCAGGCGCGATGAAAAATCCACAAACGGCAA
AAGCCGCGCAAGATATGGGCTACGAAGCCATGGTTCGACTGGCCGCGTCTCTAGAGCATCTGGAAGTGGAAGACAAAGTT
TTGCTAACCAGTTGGTTTTTGAGCAAAGCTCTGAATCAAAACCAATTTGAGCAAGCGCACTGGTGGGCGATTGGGCGATT
GGCCTCTCGTACACCGCTCTACGCAAGCCAACATCGTGTCATTCCACGCGAGCAAGCGGAACAATGGTTACCGAAACTAT
TGGATCAGAACTGGCAGAAAGAACCGATGATCGCCTTTGCTGCGGTGATGATTTGTCGTAAAACGGGTGATCGTCTCTTT
GATATCTCTGAAGATTTCCGCCAACAAGTGCTCACCAAACTGAAGCAAAGCCGAGTACCGGAAGCGTGGGTAAGCTTGGT
CGAAGAGATCAAAGAGCTTTCTGAAAGCGAGTCTAAACGCGTGTTTGGTGATGCTCTCCCGAGCGGGTTGCGTCTGGTGA
CTCAGTAA

Upstream 100 bases:

>100_bases
GTGGAATTCGACGTGCGTGACGATGCCTCTCTGGCAGAATAATCGAACAAAGCAAACGGCATCCGAAGGGTGCCGTTTTT
AAATCAATCAAGGGTTTGTC

Downstream 100 bases:

>100_bases
ACCGTCATTAGCGAACCTCCTAATCAGCCAGCCTCATGCTGGCTGATTGTTTTTCATCGTGCACCAACCAATTAGCATCG
CCCTTTCTTTGCCTGTCCCG

Product: DnaK-like protein

Products: NA

Alternate protein names: HSP70; Heat shock 70 kDa protein; Heat shock protein 70 [H]

Number of amino acids: Translated: 935; Mature: 934

Protein sequence:

>935_residues
MASPRYLVGIDLGTTNTVVAFCELTENLQQSNVSLFDIDQLIGPGEVVRKPLLPSFRYHPANGQIAPADLTLPWENQTVA
GDIANVIIGEWARELGAKVEGRQVSSAKSWLSHQAVDRHSDILPWAGVGDVEKVSPVIASASYLNHIRQAWNHRHPGNLL
EDQEVVVTVPASFDETARKLTLEAAQLAGLSQVLLLEEPQAVCYDWYARHQHSAAELLKEIPLILVCDVGGGTTDLSLIE
AKFNQDALALDRIGVGEHLMLGGDNLDLALAHLAEQRFNQSKKLNAASLTKLIQQTRKAKEQLLSSTAPEEVKITMLGSG
SKLLGGTKSISLNKQEVHQIALDGFFPLSDFTQTPGKRRSAVVEFGLPYVADPAVSKHVAEFLSQHQQVSHAALGQSESD
QPAIPVGLLLNGGVFNSDLVTARMTELLGRWRGAPVTVLDNPHPDWSVALGAVAFAKARRGAQLKIGGGSARSYFLHLQE
KNKMGKALCLLAKGTEEGQEIRLTGRRFSLTLGEPVRFNLLTSTHDALSHHVAIQNGVMADIDPDIFTPLPPYIVTLQGE
GAELEANQKERVEVQLACQLTEVGTLKMECVSVEEEDKRWALEFEVRNKKAEEQENATLHPRLNECKELITRLYSGNKKS
GDGNEIKLLAKELERKLGKRDEWDFTTLRHLFDAFAQGRKRRRRSEQHEKNWLRLAGFSLRPGFGDATDSWRIEQVWGLY
QQGIQFQNHQGWTDWWVFWRRIAGGLNQEQQETLLADIAKYLHPGAMKNPQTAKAAQDMGYEAMVRLAASLEHLEVEDKV
LLTSWFLSKALNQNQFEQAHWWAIGRLASRTPLYASQHRVIPREQAEQWLPKLLDQNWQKEPMIAFAAVMICRKTGDRLF
DISEDFRQQVLTKLKQSRVPEAWVSLVEEIKELSESESKRVFGDALPSGLRLVTQ

Sequences:

>Translated_935_residues
MASPRYLVGIDLGTTNTVVAFCELTENLQQSNVSLFDIDQLIGPGEVVRKPLLPSFRYHPANGQIAPADLTLPWENQTVA
GDIANVIIGEWARELGAKVEGRQVSSAKSWLSHQAVDRHSDILPWAGVGDVEKVSPVIASASYLNHIRQAWNHRHPGNLL
EDQEVVVTVPASFDETARKLTLEAAQLAGLSQVLLLEEPQAVCYDWYARHQHSAAELLKEIPLILVCDVGGGTTDLSLIE
AKFNQDALALDRIGVGEHLMLGGDNLDLALAHLAEQRFNQSKKLNAASLTKLIQQTRKAKEQLLSSTAPEEVKITMLGSG
SKLLGGTKSISLNKQEVHQIALDGFFPLSDFTQTPGKRRSAVVEFGLPYVADPAVSKHVAEFLSQHQQVSHAALGQSESD
QPAIPVGLLLNGGVFNSDLVTARMTELLGRWRGAPVTVLDNPHPDWSVALGAVAFAKARRGAQLKIGGGSARSYFLHLQE
KNKMGKALCLLAKGTEEGQEIRLTGRRFSLTLGEPVRFNLLTSTHDALSHHVAIQNGVMADIDPDIFTPLPPYIVTLQGE
GAELEANQKERVEVQLACQLTEVGTLKMECVSVEEEDKRWALEFEVRNKKAEEQENATLHPRLNECKELITRLYSGNKKS
GDGNEIKLLAKELERKLGKRDEWDFTTLRHLFDAFAQGRKRRRRSEQHEKNWLRLAGFSLRPGFGDATDSWRIEQVWGLY
QQGIQFQNHQGWTDWWVFWRRIAGGLNQEQQETLLADIAKYLHPGAMKNPQTAKAAQDMGYEAMVRLAASLEHLEVEDKV
LLTSWFLSKALNQNQFEQAHWWAIGRLASRTPLYASQHRVIPREQAEQWLPKLLDQNWQKEPMIAFAAVMICRKTGDRLF
DISEDFRQQVLTKLKQSRVPEAWVSLVEEIKELSESESKRVFGDALPSGLRLVTQ
>Mature_934_residues
ASPRYLVGIDLGTTNTVVAFCELTENLQQSNVSLFDIDQLIGPGEVVRKPLLPSFRYHPANGQIAPADLTLPWENQTVAG
DIANVIIGEWARELGAKVEGRQVSSAKSWLSHQAVDRHSDILPWAGVGDVEKVSPVIASASYLNHIRQAWNHRHPGNLLE
DQEVVVTVPASFDETARKLTLEAAQLAGLSQVLLLEEPQAVCYDWYARHQHSAAELLKEIPLILVCDVGGGTTDLSLIEA
KFNQDALALDRIGVGEHLMLGGDNLDLALAHLAEQRFNQSKKLNAASLTKLIQQTRKAKEQLLSSTAPEEVKITMLGSGS
KLLGGTKSISLNKQEVHQIALDGFFPLSDFTQTPGKRRSAVVEFGLPYVADPAVSKHVAEFLSQHQQVSHAALGQSESDQ
PAIPVGLLLNGGVFNSDLVTARMTELLGRWRGAPVTVLDNPHPDWSVALGAVAFAKARRGAQLKIGGGSARSYFLHLQEK
NKMGKALCLLAKGTEEGQEIRLTGRRFSLTLGEPVRFNLLTSTHDALSHHVAIQNGVMADIDPDIFTPLPPYIVTLQGEG
AELEANQKERVEVQLACQLTEVGTLKMECVSVEEEDKRWALEFEVRNKKAEEQENATLHPRLNECKELITRLYSGNKKSG
DGNEIKLLAKELERKLGKRDEWDFTTLRHLFDAFAQGRKRRRRSEQHEKNWLRLAGFSLRPGFGDATDSWRIEQVWGLYQ
QGIQFQNHQGWTDWWVFWRRIAGGLNQEQQETLLADIAKYLHPGAMKNPQTAKAAQDMGYEAMVRLAASLEHLEVEDKVL
LTSWFLSKALNQNQFEQAHWWAIGRLASRTPLYASQHRVIPREQAEQWLPKLLDQNWQKEPMIAFAAVMICRKTGDRLFD
ISEDFRQQVLTKLKQSRVPEAWVSLVEEIKELSESESKRVFGDALPSGLRLVTQ

Specific function: Acts as a chaperone [H]

COG id: COG0443

COG function: function code O; Molecular chaperone

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the heat shock protein 70 family [H]

Homologues:

Organism=Saccharomyces cerevisiae, GI6320950, Length=336, Percent_Identity=24.702380952381, Blast_Score=69, Evalue=3e-12,

Paralogues:

None

Copy number: 1300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 320 Molecules/Cell In: Early Stationary Phase, Rich Media (Based on E. coli). 500 Molecules/Cell In: Early Stationary Phase, Rich Media (Based on E. coli). 20 Molecules/Cell In: Stati

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012725
- InterPro:   IPR018181
- InterPro:   IPR001023
- InterPro:   IPR013126 [H]

Pfam domain/function: PF00012 HSP70 [H]

EC number: NA

Molecular weight: Translated: 104396; Mature: 104265

Theoretical pI: Translated: 6.55; Mature: 6.55

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASPRYLVGIDLGTTNTVVAFCELTENLQQSNVSLFDIDQLIGPGEVVRKPLLPSFRYHP
CCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCEEEHHHHCCCHHHHHCCCCCCCEECC
ANGQIAPADLTLPWENQTVAGDIANVIIGEWARELGAKVEGRQVSSAKSWLSHQAVDRHS
CCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCC
DILPWAGVGDVEKVSPVIASASYLNHIRQAWNHRHPGNLLEDQEVVVTVPASFDETARKL
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCHHHHHHH
TLEAAQLAGLSQVLLLEEPQAVCYDWYARHQHSAAELLKEIPLILVCDVGGGTTDLSLIE
HHHHHHHCCCHHEEEECCCHHHHHHHHHHHCHHHHHHHHHCCEEEEEECCCCCCCHHHHH
AKFNQDALALDRIGVGEHLMLGGDNLDLALAHLAEQRFNQSKKLNAASLTKLIQQTRKAK
HHCCCCCHHHHHCCCCCEEEECCCCHHHHHHHHHHHHHCHHHCCCHHHHHHHHHHHHHHH
EQLLSSTAPEEVKITMLGSGSKLLGGTKSISLNKQEVHQIALDGFFPLSDFTQTPGKRRS
HHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCHHHCCCCCHHH
AVVEFGLPYVADPAVSKHVAEFLSQHQQVSHAALGQSESDQPAIPVGLLLNGGVFNSDLV
HHHEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCCCCHHH
TARMTELLGRWRGAPVTVLDNPHPDWSVALGAVAFAKARRGAQLKIGGGSARSYFLHLQE
HHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHHCCCCEEEECCCCCCEEEEEEHH
KNKMGKALCLLAKGTEEGQEIRLTGRRFSLTLGEPVRFNLLTSTHDALSHHVAIQNGVMA
HCCCCCEEEEEECCCCCCCEEEEECCEEEEEECCCEEEEEEHHHHHHHHHHHHHCCCEEE
DIDPDIFTPLPPYIVTLQGEGAELEANQKERVEVQLACQLTEVGTLKMECVSVEEEDKRW
CCCCCCCCCCCCEEEEEECCCCCCCCCCCCCEEEEEEEEEHHCCCEEEEEECCCCCCCEE
ALEFEVRNKKAEEQENATLHPRLNECKELITRLYSGNKKSGDGNEIKLLAKELERKLGKR
EEEEEECCCCCHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCC
DEWDFTTLRHLFDAFAQGRKRRRRSEQHEKNWLRLAGFSLRPGFGDATDSWRIEQVWGLY
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCCCCCCHHHHHHHHH
QQGIQFQNHQGWTDWWVFWRRIAGGLNQEQQETLLADIAKYLHPGAMKNPQTAKAAQDMG
HHCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHCC
YEAMVRLAASLEHLEVEDKVLLTSWFLSKALNQNQFEQAHWWAIGRLASRTPLYASQHRV
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCC
IPREQAEQWLPKLLDQNWQKEPMIAFAAVMICRKTGDRLFDISEDFRQQVLTKLKQSRVP
CCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCEECCHHHHHHHHHHHHHHCCCC
EAWVSLVEEIKELSESESKRVFGDALPSGLRLVTQ
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCC
>Mature Secondary Structure 
ASPRYLVGIDLGTTNTVVAFCELTENLQQSNVSLFDIDQLIGPGEVVRKPLLPSFRYHP
CCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCEEEHHHHCCCHHHHHCCCCCCCEECC
ANGQIAPADLTLPWENQTVAGDIANVIIGEWARELGAKVEGRQVSSAKSWLSHQAVDRHS
CCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCC
DILPWAGVGDVEKVSPVIASASYLNHIRQAWNHRHPGNLLEDQEVVVTVPASFDETARKL
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCHHHHHHH
TLEAAQLAGLSQVLLLEEPQAVCYDWYARHQHSAAELLKEIPLILVCDVGGGTTDLSLIE
HHHHHHHCCCHHEEEECCCHHHHHHHHHHHCHHHHHHHHHCCEEEEEECCCCCCCHHHHH
AKFNQDALALDRIGVGEHLMLGGDNLDLALAHLAEQRFNQSKKLNAASLTKLIQQTRKAK
HHCCCCCHHHHHCCCCCEEEECCCCHHHHHHHHHHHHHCHHHCCCHHHHHHHHHHHHHHH
EQLLSSTAPEEVKITMLGSGSKLLGGTKSISLNKQEVHQIALDGFFPLSDFTQTPGKRRS
HHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCHHHCCCCCHHH
AVVEFGLPYVADPAVSKHVAEFLSQHQQVSHAALGQSESDQPAIPVGLLLNGGVFNSDLV
HHHEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCCCCHHH
TARMTELLGRWRGAPVTVLDNPHPDWSVALGAVAFAKARRGAQLKIGGGSARSYFLHLQE
HHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHHCCCCEEEECCCCCCEEEEEEHH
KNKMGKALCLLAKGTEEGQEIRLTGRRFSLTLGEPVRFNLLTSTHDALSHHVAIQNGVMA
HCCCCCEEEEEECCCCCCCEEEEECCEEEEEECCCEEEEEEHHHHHHHHHHHHHCCCEEE
DIDPDIFTPLPPYIVTLQGEGAELEANQKERVEVQLACQLTEVGTLKMECVSVEEEDKRW
CCCCCCCCCCCCEEEEEECCCCCCCCCCCCCEEEEEEEEEHHCCCEEEEEECCCCCCCEE
ALEFEVRNKKAEEQENATLHPRLNECKELITRLYSGNKKSGDGNEIKLLAKELERKLGKR
EEEEEECCCCCHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCC
DEWDFTTLRHLFDAFAQGRKRRRRSEQHEKNWLRLAGFSLRPGFGDATDSWRIEQVWGLY
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCCCCCCHHHHHHHHH
QQGIQFQNHQGWTDWWVFWRRIAGGLNQEQQETLLADIAKYLHPGAMKNPQTAKAAQDMG
HHCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHCC
YEAMVRLAASLEHLEVEDKVLLTSWFLSKALNQNQFEQAHWWAIGRLASRTPLYASQHRV
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCC
IPREQAEQWLPKLLDQNWQKEPMIAFAAVMICRKTGDRLFDISEDFRQQVLTKLKQSRVP
CCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCEECCHHHHHHHHHHHHHHCCCC
EAWVSLVEEIKELSESESKRVFGDALPSGLRLVTQ
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12835416 [H]