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The map label for this gene is dnaK [H]

Identifier: 27365640

GI number: 27365640

Start: 2345507

End: 2347420

Strand: Reverse

Name: dnaK [H]

Synonym: VV1_2319

Alternate gene names: 27365640

Gene position: 2347420-2345507 (Counterclockwise)

Preceding gene: 27365641

Following gene: 27365639

Centisome position: 71.53

GC content: 53.29

Gene sequence:

>1914_bases
ATGGAAACGATGATGACACCCGCAACCGAGCAAGTGGCAAAATTCAGCGTTGGTATCGACTTAGGTACCACGCACTGTGT
TATGTCATTCATGGATACCCAGGATGAGCAAGCTCGCGTACAAGTGATGGCCATTCCACAATTGACGGCGCCTGGCACCG
TTGAGTCTCGTAACCAACTTGGTTCGTTTCTTTACCAGCCGCATGAGCATGAAATGAACCCTGCGTCACGCGTGTTGCCA
TGGACGACTGAGCCAACTGCCCTTGTCGGCGCAATCGCACGTAATTTGGGTGGTAAAACGCCTTTGCGTTTGGTAGCCAG
TGCGAAATCTTGGCTTTGCCATGGTGGCGTGAATCGCCGCGATGCGTTTCTACCAGCTGGCAGCCCAGAAGAGATTGAAA
AAGTCTCACCACTGCGCGCAACCGAGCTTTATTTAGAGCATCTTAAGCAGGCGTGGGATCACGCACAGCCAAACCATCCG
CTATCAGAGCAAGACGTGACCATCACAGTGCCGGCATCGTTTGATCCTGCGGCGCGCGATCTGACGGCAGAAGCGGCGCG
CAATGTCGGTTTTGTCCATCTTACCTTGTTGGAAGAGCCTCAAGCCGCGCTTTACAACTGGATCGACAACAGCAATGACA
AATGGCGCGATGAAGTCACTGTGGGTGATATCGTGCTGGTGGTGGACATCGGCGGTGGTACTACTGACCTTTCTTTGGTG
GAAGTGACCGAAGAAGAAGGTAATCTGACCCTCAAACGCATCGCTGTCGGTGAACACATTCTGCTGGGCGGTGACAACAT
GGATCTTGCCCTCGCCTACAGTTTGAAGATGAAGCTGGCTCAGGAAGGCAAAGAGCTGCAACCTTGGCAAGTACAAGCCA
TGACGCACGCTTGCCGCGACGCGAAAGAAGCGCTGCTCAATGATGCAGAACTGCAATCGGTACCGATTGTCGTGCCAAGC
CGCGGTTCTAAATTGCTCGGCGCTACGCTGAAAACCGAACTCACGCAGCAAGAAGTGCAGCAAATGCTCGTCGATGGCTT
TTTCCCTAAAGTCGCTATCACCGAACACCCAGTACAACGTAACCGCGGTGCACTGACCCAGATGGGCTTGCCTTATGCAC
AAGACGCAGGCATCACTCGCCACATTGCGGCTTTCTTGTCGAAGCAAGCCAATGCCAACGGTGAAGCGGTACCTGCACAA
GATTACAACCCGTTTGTTGGCGGAATGCCGGGTATGCCTGGCGCTCAACAAGCTTCTGTCGATTTCATCAAACCTACCGC
GATTTTGTTCAATGGTGGTGTGCTGAAGTCAGCTCTCTTAGCAGAACGCTTAGAAGAAACCATCAACGAATGGTTGCTTG
ACGCTGATGCAGAGATGGCAAAACGACTCACCGGCGTCGATTTGGATCTCGCGGTGGCGAGCGGCGCGGCTTACTATGGC
GCAGTGCGTCGTGGCCAAGGTGTGCGTATTCGCGGTGGTATTGCTTCAAGCTATTACGTCGGTATCGAGAGCGCAATGCC
TGCCATTCCAGGTATGGCACCGCCAATGGAAGCACTGTGTGTGGCGCCGTTTGGCATGGAAGAAGGCACCAGCGTCGATG
TACCAAGTCAGCAGTTTGGTTTGATCATCGGCCAGCCAGTGAATTTCCAATTCTTTGGCTCAACGGTTCGTCGTGACGAT
CTTACAGGAACACATTTGGACCATTGGGGCCCAGATGAACTTGAAGAATTACCAGAGATTCAAGTGACGTTACCGGTTTC
AGAGGGTCGCCGTGAAGGCGAAGTGGTGCCGGTGACATTGGCCTCACGCGTGACTGAACTTGGCACGCTGTATTTAGAAG
CGATTGCGGCGGATAACGGTCAAAAATGGCACGTGGAATTCGACGTGCGTGACGATGCCTCTCTGGCAGAATAA

Upstream 100 bases:

>100_bases
CCTTAATCCACAAAGGTTGGAAAGCTTCTGCAATGACGCTACCGAAACTAGCGGAAAACTACGACGCTTCGATCATCGCA
CCCGCTGAGGTTGAGCTGTA

Downstream 100 bases:

>100_bases
TCGAACAAAGCAAACGGCATCCGAAGGGTGCCGTTTTTAAATCAATCAAGGGTTTGTCATGGCATCTCCTCGCTATCTTG
TCGGCATTGACTTAGGCACC

Product: DnaK-like protein

Products: NA

Alternate protein names: HSP70; Heat shock 70 kDa protein; Heat shock protein 70 [H]

Number of amino acids: Translated: 637; Mature: 637

Protein sequence:

>637_residues
METMMTPATEQVAKFSVGIDLGTTHCVMSFMDTQDEQARVQVMAIPQLTAPGTVESRNQLGSFLYQPHEHEMNPASRVLP
WTTEPTALVGAIARNLGGKTPLRLVASAKSWLCHGGVNRRDAFLPAGSPEEIEKVSPLRATELYLEHLKQAWDHAQPNHP
LSEQDVTITVPASFDPAARDLTAEAARNVGFVHLTLLEEPQAALYNWIDNSNDKWRDEVTVGDIVLVVDIGGGTTDLSLV
EVTEEEGNLTLKRIAVGEHILLGGDNMDLALAYSLKMKLAQEGKELQPWQVQAMTHACRDAKEALLNDAELQSVPIVVPS
RGSKLLGATLKTELTQQEVQQMLVDGFFPKVAITEHPVQRNRGALTQMGLPYAQDAGITRHIAAFLSKQANANGEAVPAQ
DYNPFVGGMPGMPGAQQASVDFIKPTAILFNGGVLKSALLAERLEETINEWLLDADAEMAKRLTGVDLDLAVASGAAYYG
AVRRGQGVRIRGGIASSYYVGIESAMPAIPGMAPPMEALCVAPFGMEEGTSVDVPSQQFGLIIGQPVNFQFFGSTVRRDD
LTGTHLDHWGPDELEELPEIQVTLPVSEGRREGEVVPVTLASRVTELGTLYLEAIAADNGQKWHVEFDVRDDASLAE

Sequences:

>Translated_637_residues
METMMTPATEQVAKFSVGIDLGTTHCVMSFMDTQDEQARVQVMAIPQLTAPGTVESRNQLGSFLYQPHEHEMNPASRVLP
WTTEPTALVGAIARNLGGKTPLRLVASAKSWLCHGGVNRRDAFLPAGSPEEIEKVSPLRATELYLEHLKQAWDHAQPNHP
LSEQDVTITVPASFDPAARDLTAEAARNVGFVHLTLLEEPQAALYNWIDNSNDKWRDEVTVGDIVLVVDIGGGTTDLSLV
EVTEEEGNLTLKRIAVGEHILLGGDNMDLALAYSLKMKLAQEGKELQPWQVQAMTHACRDAKEALLNDAELQSVPIVVPS
RGSKLLGATLKTELTQQEVQQMLVDGFFPKVAITEHPVQRNRGALTQMGLPYAQDAGITRHIAAFLSKQANANGEAVPAQ
DYNPFVGGMPGMPGAQQASVDFIKPTAILFNGGVLKSALLAERLEETINEWLLDADAEMAKRLTGVDLDLAVASGAAYYG
AVRRGQGVRIRGGIASSYYVGIESAMPAIPGMAPPMEALCVAPFGMEEGTSVDVPSQQFGLIIGQPVNFQFFGSTVRRDD
LTGTHLDHWGPDELEELPEIQVTLPVSEGRREGEVVPVTLASRVTELGTLYLEAIAADNGQKWHVEFDVRDDASLAE
>Mature_637_residues
METMMTPATEQVAKFSVGIDLGTTHCVMSFMDTQDEQARVQVMAIPQLTAPGTVESRNQLGSFLYQPHEHEMNPASRVLP
WTTEPTALVGAIARNLGGKTPLRLVASAKSWLCHGGVNRRDAFLPAGSPEEIEKVSPLRATELYLEHLKQAWDHAQPNHP
LSEQDVTITVPASFDPAARDLTAEAARNVGFVHLTLLEEPQAALYNWIDNSNDKWRDEVTVGDIVLVVDIGGGTTDLSLV
EVTEEEGNLTLKRIAVGEHILLGGDNMDLALAYSLKMKLAQEGKELQPWQVQAMTHACRDAKEALLNDAELQSVPIVVPS
RGSKLLGATLKTELTQQEVQQMLVDGFFPKVAITEHPVQRNRGALTQMGLPYAQDAGITRHIAAFLSKQANANGEAVPAQ
DYNPFVGGMPGMPGAQQASVDFIKPTAILFNGGVLKSALLAERLEETINEWLLDADAEMAKRLTGVDLDLAVASGAAYYG
AVRRGQGVRIRGGIASSYYVGIESAMPAIPGMAPPMEALCVAPFGMEEGTSVDVPSQQFGLIIGQPVNFQFFGSTVRRDD
LTGTHLDHWGPDELEELPEIQVTLPVSEGRREGEVVPVTLASRVTELGTLYLEAIAADNGQKWHVEFDVRDDASLAE

Specific function: Acts as a chaperone [H]

COG id: COG0443

COG function: function code O; Molecular chaperone

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the heat shock protein 70 family [H]

Homologues:

Organism=Escherichia coli, GI1786196, Length=304, Percent_Identity=25.6578947368421, Blast_Score=70, Evalue=3e-13,

Paralogues:

None

Copy number: 1300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 320 Molecules/Cell In: Early Stationary Phase, Rich Media (Based on E. coli). 500 Molecules/Cell In: Early Stationary Phase, Rich Media (Based on E. coli). 20 Molecules/Cell In: Stati

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012725
- InterPro:   IPR018181
- InterPro:   IPR001023
- InterPro:   IPR013126 [H]

Pfam domain/function: PF00012 HSP70 [H]

EC number: NA

Molecular weight: Translated: 69015; Mature: 69015

Theoretical pI: Translated: 4.48; Mature: 4.48

Prosite motif: PS00297 HSP70_1 ; PS00329 HSP70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
METMMTPATEQVAKFSVGIDLGTTHCVMSFMDTQDEQARVQVMAIPQLTAPGTVESRNQL
CCCCCCCCHHHHHHEEECCCCCHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCCCCHHHH
GSFLYQPHEHEMNPASRVLPWTTEPTALVGAIARNLGGKTPLRLVASAKSWLCHGGVNRR
HHHHCCCCCCCCCHHHCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCC
DAFLPAGSPEEIEKVSPLRATELYLEHLKQAWDHAQPNHPLSEQDVTITVPASFDPAARD
CCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCCHHHH
LTAEAARNVGFVHLTLLEEPQAALYNWIDNSNDKWRDEVTVGDIVLVVDIGGGTTDLSLV
HHHHHHHCCCEEEEEEECCCHHHHHHHHCCCCCCCCCCEEECCEEEEEECCCCCCCEEEE
EVTEEEGNLTLKRIAVGEHILLGGDNMDLALAYSLKMKLAQEGKELQPWQVQAMTHACRD
EEECCCCCEEEEEEECCCEEEECCCCCCEEEEEHHHHHHHHCCCCCCCHHHHHHHHHHHH
AKEALLNDAELQSVPIVVPSRGSKLLGATLKTELTQQEVQQMLVDGFFPKVAITEHPVQR
HHHHHHCCCCCCCCCEEECCCCCEEHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHH
NRGALTQMGLPYAQDAGITRHIAAFLSKQANANGEAVPAQDYNPFVGGMPGMPGAQQASV
CCCCHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
DFIKPTAILFNGGVLKSALLAERLEETINEWLLDADAEMAKRLTGVDLDLAVASGAAYYG
EEECCEEEEEECCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCEEEEECCCCHHHH
AVRRGQGVRIRGGIASSYYVGIESAMPAIPGMAPPMEALCVAPFGMEEGTSVDVPSQQFG
HHHCCCCEEEECCCCCEEEEEHHHHCCCCCCCCCCHHHHEECCCCCCCCCCCCCCCHHCC
LIIGQPVNFQFFGSTVRRDDLTGTHLDHWGPDELEELPEIQVTLPVSEGRREGEVVPVTL
EEEECCCCEEEECCCCCCCCCCCCCCCCCCHHHHHHCCCEEEEEEHHCCCCCCCEEEEEH
ASRVTELGTLYLEAIAADNGQKWHVEFDVRDDASLAE
HHHHHHHHHHHHHEEECCCCCEEEEEEECCCCCCCCC
>Mature Secondary Structure
METMMTPATEQVAKFSVGIDLGTTHCVMSFMDTQDEQARVQVMAIPQLTAPGTVESRNQL
CCCCCCCCHHHHHHEEECCCCCHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCCCCHHHH
GSFLYQPHEHEMNPASRVLPWTTEPTALVGAIARNLGGKTPLRLVASAKSWLCHGGVNRR
HHHHCCCCCCCCCHHHCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCC
DAFLPAGSPEEIEKVSPLRATELYLEHLKQAWDHAQPNHPLSEQDVTITVPASFDPAARD
CCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCCHHHH
LTAEAARNVGFVHLTLLEEPQAALYNWIDNSNDKWRDEVTVGDIVLVVDIGGGTTDLSLV
HHHHHHHCCCEEEEEEECCCHHHHHHHHCCCCCCCCCCEEECCEEEEEECCCCCCCEEEE
EVTEEEGNLTLKRIAVGEHILLGGDNMDLALAYSLKMKLAQEGKELQPWQVQAMTHACRD
EEECCCCCEEEEEEECCCEEEECCCCCCEEEEEHHHHHHHHCCCCCCCHHHHHHHHHHHH
AKEALLNDAELQSVPIVVPSRGSKLLGATLKTELTQQEVQQMLVDGFFPKVAITEHPVQR
HHHHHHCCCCCCCCCEEECCCCCEEHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHH
NRGALTQMGLPYAQDAGITRHIAAFLSKQANANGEAVPAQDYNPFVGGMPGMPGAQQASV
CCCCHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
DFIKPTAILFNGGVLKSALLAERLEETINEWLLDADAEMAKRLTGVDLDLAVASGAAYYG
EEECCEEEEEECCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCEEEEECCCCHHHH
AVRRGQGVRIRGGIASSYYVGIESAMPAIPGMAPPMEALCVAPFGMEEGTSVDVPSQQFG
HHHCCCCEEEECCCCCEEEEEHHHHCCCCCCCCCCHHHHEECCCCCCCCCCCCCCCHHCC
LIIGQPVNFQFFGSTVRRDDLTGTHLDHWGPDELEELPEIQVTLPVSEGRREGEVVPVTL
EEEECCCCEEEECCCCCCCCCCCCCCCCCCHHHHHHCCCEEEEEEHHCCCCCCCEEEEEH
ASRVTELGTLYLEAIAADNGQKWHVEFDVRDDASLAE
HHHHHHHHHHHHHEEECCCCCEEEEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12835416 [H]