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The map label for this gene is purK

Identifier: 27364485

GI number: 27364485

Start: 1055193

End: 1056326

Strand: Reverse

Name: purK

Synonym: VV1_1053

Alternate gene names: 27364485

Gene position: 1056326-1055193 (Counterclockwise)

Preceding gene: 27364486

Following gene: 27364480

Centisome position: 32.19

GC content: 48.06

Gene sequence:

>1134_bases
ATGCACGTATTGGTTCTAGGCGCAGGGCAATTGGCACGTATGATGTCGTTAGCCGGTGCGCCTTTGAATATTCAGATTTC
TGCCTACGATGTGACAACGGGTGATGTCGTTCATCCTTTGACATTGCATCTCCTTGGTCACGGCTTGGAGCAAGCGATTG
AATACGTTGATGTGATCACTGCAGAATTTGAACACATTCCTCATGACGTATTGGCGATCTGCCAAGCGAGTGGCAAGTTT
CTTCCAAGTAGTGAGGCGATTAAGGCTGGCGGTGATCGTCGTTTGGAAAAAGCCTTGCTTGACCATGCAGGCGTACGCAA
TGCCAATTACTACGTCATTGAAACGCGTGAAGACTTCAATAAAGCGATTGAACACGTTGGGATACCTATGGTGTTGAAAA
GCGCATTAGGTGGCTATGATGGTAAAGGGCAATGGCGCTTAAAAGATGCCGCACAAATTGAAACCCTTTGGCAAGAAATG
GCAGCTTGTATCGCTGCTACGCCAACTCAAGCGATTGTCGCTGAAGAATTTGTTCCATTTCAACGTGAAGTTTCATTGAT
CGGCGCTCGTGGTAAAGAAGGGCAAATCGAAGTCTACCCACTGGCAGAAAACATTCACGTTAATGGCGTGTTAAGCCTTT
CAACGGCAATCGACTCACCGGACCTACAAGAGCAAGCCAAACACATGTTTACTGCTGTTGCTGAAACATTGAACTACGTT
GGTGTGTTGGCGCTTGAATTTTTCGATGTCGACGGACAATTGCTGGTCAATGAAATTGCACCTCGAGTTCATAACTCCGG
CCATTGGACCCAGCAAGGAGCAGAAACTTGCCAGTTTGAAAATCATTTGCGTGCCGTTTGTGGCTTACCACTTGGGAGCA
CTAAGCTGGTTCGTGAAACCTCAATGATCAACATCCTTGGTGAAGATACTCTGCCCGCAAGCGTGATGGCGATGGACGGT
TGTCATATTCATTGGTATGGCAAAGAGAAACGCGCCGGCCGAAAAATGGGCCATATCAACGTGTGTGGTGATTACAGCGG
AGAGCTACAGCGCCGCCTTTGTGCTCTCGCCAATGTATTGGATGAGAAAGCCTTCCCCGCGGTGCATGAGTTTGCAAAAA
AGTGGCAAGCGTAG

Upstream 100 bases:

>100_bases
TGGAACTCACGACGAAAATATCATGGCCAAAGTTGAAGCCTTCCGCAGTGAGCAAACTGAAAGCGTCCTCGCTAACCCAA
ATCCTGCAGAGGACTAAATC

Downstream 100 bases:

>100_bases
CAAAGCTTGTGCTTATGCATAAAGAGTAAAAGGGGCGCCATGTGCGACCCTTTTCAATGTGAACTCAACGCGATTGAGTA
TGCTGGCATTTGCGGCTTGC

Product: phosphoribosylaminoimidazole carboxylase ATPase subunit

Products: NA

Alternate protein names: N5-CAIR synthase; 5-(carboxyamino)imidazole ribonucleotide synthetase

Number of amino acids: Translated: 377; Mature: 377

Protein sequence:

>377_residues
MHVLVLGAGQLARMMSLAGAPLNIQISAYDVTTGDVVHPLTLHLLGHGLEQAIEYVDVITAEFEHIPHDVLAICQASGKF
LPSSEAIKAGGDRRLEKALLDHAGVRNANYYVIETREDFNKAIEHVGIPMVLKSALGGYDGKGQWRLKDAAQIETLWQEM
AACIAATPTQAIVAEEFVPFQREVSLIGARGKEGQIEVYPLAENIHVNGVLSLSTAIDSPDLQEQAKHMFTAVAETLNYV
GVLALEFFDVDGQLLVNEIAPRVHNSGHWTQQGAETCQFENHLRAVCGLPLGSTKLVRETSMINILGEDTLPASVMAMDG
CHIHWYGKEKRAGRKMGHINVCGDYSGELQRRLCALANVLDEKAFPAVHEFAKKWQA

Sequences:

>Translated_377_residues
MHVLVLGAGQLARMMSLAGAPLNIQISAYDVTTGDVVHPLTLHLLGHGLEQAIEYVDVITAEFEHIPHDVLAICQASGKF
LPSSEAIKAGGDRRLEKALLDHAGVRNANYYVIETREDFNKAIEHVGIPMVLKSALGGYDGKGQWRLKDAAQIETLWQEM
AACIAATPTQAIVAEEFVPFQREVSLIGARGKEGQIEVYPLAENIHVNGVLSLSTAIDSPDLQEQAKHMFTAVAETLNYV
GVLALEFFDVDGQLLVNEIAPRVHNSGHWTQQGAETCQFENHLRAVCGLPLGSTKLVRETSMINILGEDTLPASVMAMDG
CHIHWYGKEKRAGRKMGHINVCGDYSGELQRRLCALANVLDEKAFPAVHEFAKKWQA
>Mature_377_residues
MHVLVLGAGQLARMMSLAGAPLNIQISAYDVTTGDVVHPLTLHLLGHGLEQAIEYVDVITAEFEHIPHDVLAICQASGKF
LPSSEAIKAGGDRRLEKALLDHAGVRNANYYVIETREDFNKAIEHVGIPMVLKSALGGYDGKGQWRLKDAAQIETLWQEM
AACIAATPTQAIVAEEFVPFQREVSLIGARGKEGQIEVYPLAENIHVNGVLSLSTAIDSPDLQEQAKHMFTAVAETLNYV
GVLALEFFDVDGQLLVNEIAPRVHNSGHWTQQGAETCQFENHLRAVCGLPLGSTKLVRETSMINILGEDTLPASVMAMDG
CHIHWYGKEKRAGRKMGHINVCGDYSGELQRRLCALANVLDEKAFPAVHEFAKKWQA

Specific function: Catalyzes the ATP-dependent conversion of 5- aminoimidazole ribonucleotide (AIR) and HCO(3)(-) to N5- carboxyaminoimidazole ribonucleotide (N5-CAIR)

COG id: COG0026

COG function: function code F; Phosphoribosylaminoimidazole carboxylase (NCAIR synthetase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain

Homologues:

Organism=Escherichia coli, GI1786733, Length=361, Percent_Identity=36.5650969529086, Blast_Score=204, Evalue=6e-54,
Organism=Escherichia coli, GI1788155, Length=306, Percent_Identity=23.202614379085, Blast_Score=72, Evalue=7e-14,
Organism=Saccharomyces cerevisiae, GI6324702, Length=383, Percent_Identity=30.2872062663185, Blast_Score=157, Evalue=2e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PURK_VIBVU (Q8DDD8)

Other databases:

- EMBL:   AE016795
- RefSeq:   NP_760013.1
- ProteinModelPortal:   Q8DDD8
- SMR:   Q8DDD8
- GeneID:   1177986
- GenomeReviews:   AE016795_GR
- KEGG:   vvu:VV1_1053
- HOGENOM:   HBG516369
- OMA:   LAVEMFV
- ProtClustDB:   PRK06019
- BioCyc:   VVUL216895:VV1_1053-MONOMER
- BRENDA:   4.1.1.21
- InterPro:   IPR005875
- InterPro:   IPR011761
- InterPro:   IPR003135
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR011054
- Gene3D:   G3DSA:3.30.1490.20
- Gene3D:   G3DSA:3.30.470.20
- Gene3D:   G3DSA:3.40.50.20
- PANTHER:   PTHR23047:SF1
- TIGRFAMs:   TIGR01161

Pfam domain/function: PF02222 ATP-grasp; SSF52440 PreATP-grasp-like; SSF51246 Rudmnt_hyb_motif

EC number: =6.3.4.18

Molecular weight: Translated: 41232; Mature: 41232

Theoretical pI: Translated: 5.79; Mature: 5.79

Prosite motif: PS50975 ATP_GRASP

Important sites: BINDING 93-93 BINDING 133-133 BINDING 144-144 BINDING 183-183

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHVLVLGAGQLARMMSLAGAPLNIQISAYDVTTGDVVHPLTLHLLGHGLEQAIEYVDVIT
CEEEEECCHHHHHHHHHCCCCEEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHH
AEFEHIPHDVLAICQASGKFLPSSEAIKAGGDRRLEKALLDHAGVRNANYYVIETREDFN
HHHHHCCHHHHHHHHHCCCCCCCCHHHHCCCHHHHHHHHHHHCCCCCCCEEEEECHHHHH
KAIEHVGIPMVLKSALGGYDGKGQWRLKDAAQIETLWQEMAACIAATPTQAIVAEEFVPF
HHHHHCCHHHHHHHHHCCCCCCCCEEECHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCH
QREVSLIGARGKEGQIEVYPLAENIHVNGVLSLSTAIDSPDLQEQAKHMFTAVAETLNYV
HHHHHHEECCCCCCCEEEEEECCCEEECEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHH
GVLALEFFDVDGQLLVNEIAPRVHNSGHWTQQGAETCQFENHLRAVCGLPLGSTKLVRET
HHHHHEEECCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
SMINILGEDTLPASVMAMDGCHIHWYGKEKRAGRKMGHINVCGDYSGELQRRLCALANVL
HHEEEECCCCCCHHHEEECCCEEEEECCHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHH
DEKAFPAVHEFAKKWQA
HHHCCHHHHHHHHHHCC
>Mature Secondary Structure
MHVLVLGAGQLARMMSLAGAPLNIQISAYDVTTGDVVHPLTLHLLGHGLEQAIEYVDVIT
CEEEEECCHHHHHHHHHCCCCEEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHH
AEFEHIPHDVLAICQASGKFLPSSEAIKAGGDRRLEKALLDHAGVRNANYYVIETREDFN
HHHHHCCHHHHHHHHHCCCCCCCCHHHHCCCHHHHHHHHHHHCCCCCCCEEEEECHHHHH
KAIEHVGIPMVLKSALGGYDGKGQWRLKDAAQIETLWQEMAACIAATPTQAIVAEEFVPF
HHHHHCCHHHHHHHHHCCCCCCCCEEECHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCH
QREVSLIGARGKEGQIEVYPLAENIHVNGVLSLSTAIDSPDLQEQAKHMFTAVAETLNYV
HHHHHHEECCCCCCCEEEEEECCCEEECEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHH
GVLALEFFDVDGQLLVNEIAPRVHNSGHWTQQGAETCQFENHLRAVCGLPLGSTKLVRET
HHHHHEEECCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
SMINILGEDTLPASVMAMDGCHIHWYGKEKRAGRKMGHINVCGDYSGELQRRLCALANVL
HHEEEECCCCCCHHHEEECCCEEEEECCHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHH
DEKAFPAVHEFAKKWQA
HHHCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA