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The map label for this gene is iolG

Identifier: 255767850

GI number: 255767850

Start: 4075785

End: 4076819

Strand: Reverse

Name: iolG

Synonym: BSU39700

Alternate gene names: 255767850

Gene position: 4076819-4075785 (Counterclockwise)

Preceding gene: 255767851

Following gene: 16081020

Centisome position: 96.71

GC content: 45.31

Gene sequence:

>1035_bases
ATGAGTTTACGTATTGGCGTAATTGGAACTGGAGCAATCGGAAAAGAACATATTAACCGTATCACGAACAAGCTGTCAGG
CGCGGAAATTGTAGCTGTAACGGATGTTAATCAAGAAGCTGCACAAAAGGTCGTTGAGCAATACCAATTAAACGCGACGG
TTTATCCGAATGATGACAGCTTGCTTGCAGACGAAAATGTAGACGCTGTTTTAGTGACAAGCTGGGGGCCTGCGCATGAG
TCAAGCGTGCTGAAAGCGATTAAAGCCCAGAAATATGTGTTCTGTGAAAAACCGCTCGCGACAACGGCTGAAGGATGCAT
GCGCATTGTCGAAGAAGAAATCAAAGTGGGCAAACGCCTTGTTCAAGTCGGCTTCATGCGCCGTTATGACAGCGGTTACG
TACAGCTGAAAGAAGCGCTCGATAATCATGTCATCGGCGAGCCTCTTATGATTCACTGCGCGCACCGCAACCCGACTGTA
GGAGATAACTATACAACGGATATGGCTGTAGTCGACACGCTTGTTCATGAAATTGACGTGCTCCACTGGCTCGTCAATGA
TGACTACGAGTCCGTTCAAGTCATCTATCCGAAAAAATCAAAAAACGCGCTTCCACATTTAAAAGATCCGCAAATCGTCG
TGATTGAAACAAAAGGCGGTATCGTCATCAATGCTGAAATCTATGTGAACTGTAAATACGGCTATGACATTCAATGTGAA
ATCGTCGGAGAAGACGGCATCATCAAGCTTCCCGAGCCATCAAGCATCAGCTTGAGAAAAGAAGGCAGATTCAGCACTGA
TATTTTGATGGATTGGCAGAGACGCTTTGTCGCTGCGTATGATGTGGAAATCCAAGACTTTATTGATTCGATTCAAAAGA
AAGGCGAGGTCAGCGGACCGACGGCATGGGACGGCTATATTGCTGCTGTCACGACTGACGCGTGTGTAAAAGCCCAGGAA
TCTGGACAAAAAGAAAAGGTTGAATTGAAGGAAAAACCGGAATTCTATCAATCTTTTACAACAGTTCAAAACTAA

Upstream 100 bases:

>100_bases
CTTGAACAAATTCAAGAGGAACTCTACGGGTCTCCTCAGAGCCAAGTAAAGAAAGGAACAGAAAGCAAAATCATGTAACC
AACAGAAGGAGTGGCTGTCA

Downstream 100 bases:

>100_bases
AAAACAGAGGAGTGAAAATATGAAGTTAGCATTGGATCCATCCATGTATCGTGATGATTTAACTTTAGAGGAAATGGTCT
ATAAAACGGCAGAGCTCGGC

Product: myo-inositol 2-dehydrogenase

Products: NA

Alternate protein names: Myo-inositol 2-dehydrogenase/D-chiro-inositol 3-dehydrogenase; MI 2-dehydrogenase/DCI 3-dehydrogenase

Number of amino acids: Translated: 344; Mature: 343

Protein sequence:

>344_residues
MSLRIGVIGTGAIGKEHINRITNKLSGAEIVAVTDVNQEAAQKVVEQYQLNATVYPNDDSLLADENVDAVLVTSWGPAHE
SSVLKAIKAQKYVFCEKPLATTAEGCMRIVEEEIKVGKRLVQVGFMRRYDSGYVQLKEALDNHVIGEPLMIHCAHRNPTV
GDNYTTDMAVVDTLVHEIDVLHWLVNDDYESVQVIYPKKSKNALPHLKDPQIVVIETKGGIVINAEIYVNCKYGYDIQCE
IVGEDGIIKLPEPSSISLRKEGRFSTDILMDWQRRFVAAYDVEIQDFIDSIQKKGEVSGPTAWDGYIAAVTTDACVKAQE
SGQKEKVELKEKPEFYQSFTTVQN

Sequences:

>Translated_344_residues
MSLRIGVIGTGAIGKEHINRITNKLSGAEIVAVTDVNQEAAQKVVEQYQLNATVYPNDDSLLADENVDAVLVTSWGPAHE
SSVLKAIKAQKYVFCEKPLATTAEGCMRIVEEEIKVGKRLVQVGFMRRYDSGYVQLKEALDNHVIGEPLMIHCAHRNPTV
GDNYTTDMAVVDTLVHEIDVLHWLVNDDYESVQVIYPKKSKNALPHLKDPQIVVIETKGGIVINAEIYVNCKYGYDIQCE
IVGEDGIIKLPEPSSISLRKEGRFSTDILMDWQRRFVAAYDVEIQDFIDSIQKKGEVSGPTAWDGYIAAVTTDACVKAQE
SGQKEKVELKEKPEFYQSFTTVQN
>Mature_343_residues
SLRIGVIGTGAIGKEHINRITNKLSGAEIVAVTDVNQEAAQKVVEQYQLNATVYPNDDSLLADENVDAVLVTSWGPAHES
SVLKAIKAQKYVFCEKPLATTAEGCMRIVEEEIKVGKRLVQVGFMRRYDSGYVQLKEALDNHVIGEPLMIHCAHRNPTVG
DNYTTDMAVVDTLVHEIDVLHWLVNDDYESVQVIYPKKSKNALPHLKDPQIVVIETKGGIVINAEIYVNCKYGYDIQCEI
VGEDGIIKLPEPSSISLRKEGRFSTDILMDWQRRFVAAYDVEIQDFIDSIQKKGEVSGPTAWDGYIAAVTTDACVKAQES
GQKEKVELKEKPEFYQSFTTVQN

Specific function: Involved in the oxidation of myo-inositol (MI) and D- chiro-inositol (DCI) to 2-keto-myo-inositol (2KMI or 2-inosose) and 1-keto-D-chiro-inositol (1KDCI), respectively. Can also use D- glucose and D-xylose, and shows a trace of activity with D-ribose and

COG id: COG0673

COG function: function code R; Predicted dehydrogenases and related proteins

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gfo/idh/mocA family

Homologues:

Organism=Escherichia coli, GI1787574, Length=146, Percent_Identity=33.5616438356164, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI87082405, Length=151, Percent_Identity=30.4635761589404, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): IOLG_BACSU (P26935)

Other databases:

- EMBL:   M76431
- EMBL:   D14399
- EMBL:   AY676876
- EMBL:   AL009126
- PIR:   JH0511
- RefSeq:   NP_391849.2
- PDB:   3MZ0
- PDB:   3NT2
- PDB:   3NT4
- PDB:   3NT5
- PDB:   3NTO
- PDB:   3NTQ
- PDB:   3NTR
- PDBsum:   3MZ0
- PDBsum:   3NT2
- PDBsum:   3NT4
- PDBsum:   3NT5
- PDBsum:   3NTO
- PDBsum:   3NTQ
- PDBsum:   3NTR
- ProteinModelPortal:   P26935
- SMR:   P26935
- EnsemblBacteria:   EBBACT00000000632
- GeneID:   937615
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU39700
- NMPDR:   fig|224308.1.peg.3975
- GenoList:   BSU39700
- GeneTree:   EBGT00050000000829
- HOGENOM:   HBG612336
- ProtClustDB:   CLSK873287
- BioCyc:   BSUB:BSU39700-MONOMER
- BRENDA:   1.1.1.18
- GO:   GO:0005488
- HAMAP:   MF_01671
- InterPro:   IPR016040
- InterPro:   IPR000683
- InterPro:   IPR004104
- Gene3D:   G3DSA:3.40.50.720

Pfam domain/function: PF01408 GFO_IDH_MocA; PF02894 GFO_IDH_MocA_C

EC number: =1.1.1.18

Molecular weight: Translated: 38351; Mature: 38220

Theoretical pI: Translated: 4.81; Mature: 4.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLRIGVIGTGAIGKEHINRITNKLSGAEIVAVTDVNQEAAQKVVEQYQLNATVYPNDDS
CEEEEEEEECCCCCHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHCCEEEECCCCC
LLADENVDAVLVTSWGPAHESSVLKAIKAQKYVFCEKPLATTAEGCMRIVEEEIKVGKRL
EEECCCCCEEEEECCCCCHHHHHHHHHHHHHEEEECCCCHHHHHHHHHHHHHHHHHHHHH
VQVGFMRRYDSGYVQLKEALDNHVIGEPLMIHCAHRNPTVGDNYTTDMAVVDTLVHEIDV
HHHHHHHHCCCCHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHH
LHWLVNDDYESVQVIYPKKSKNALPHLKDPQIVVIETKGGIVINAEIYVNCKYGYDIQCE
HHHEECCCCCCEEEEECCCCCCCCCCCCCCCEEEEEECCCEEEEEEEEEEEECCCEEEEE
IVGEDGIIKLPEPSSISLRKEGRFSTDILMDWQRRFVAAYDVEIQDFIDSIQKKGEVSGP
EECCCCEEECCCCCCCEEECCCCCCHHHHHHHHHHEEEEECCCHHHHHHHHHHCCCCCCC
TAWDGYIAAVTTDACVKAQESGQKEKVELKEKPEFYQSFTTVQN
CCCCCEEEEEEHHHHHHHHHCCCCCEECCCCCHHHHHHHHHCCC
>Mature Secondary Structure 
SLRIGVIGTGAIGKEHINRITNKLSGAEIVAVTDVNQEAAQKVVEQYQLNATVYPNDDS
EEEEEEEECCCCCHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHCCEEEECCCCC
LLADENVDAVLVTSWGPAHESSVLKAIKAQKYVFCEKPLATTAEGCMRIVEEEIKVGKRL
EEECCCCCEEEEECCCCCHHHHHHHHHHHHHEEEECCCCHHHHHHHHHHHHHHHHHHHHH
VQVGFMRRYDSGYVQLKEALDNHVIGEPLMIHCAHRNPTVGDNYTTDMAVVDTLVHEIDV
HHHHHHHHCCCCHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHH
LHWLVNDDYESVQVIYPKKSKNALPHLKDPQIVVIETKGGIVINAEIYVNCKYGYDIQCE
HHHEECCCCCCEEEEECCCCCCCCCCCCCCCEEEEEECCCEEEEEEEEEEEECCCEEEEE
IVGEDGIIKLPEPSSISLRKEGRFSTDILMDWQRRFVAAYDVEIQDFIDSIQKKGEVSGP
EECCCCEEECCCCCCCEEECCCCCCHHHHHHHHHHEEEEECCCHHHHHHHHHHCCCCCCC
TAWDGYIAAVTTDACVKAQESGQKEKVELKEKPEFYQSFTTVQN
CCCCCEEEEEEHHHHHHHHHCCCCCEECCCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1761221; 7952181; 9384377; 112095