Click here to switch to the map view.

The map label for this gene is ywaA

Identifier: 255767817

GI number: 255767817

Start: 3957391

End: 3958482

Strand: Direct

Name: ywaA

Synonym: BSU38550

Alternate gene names: 255767817

Gene position: 3957391-3958482 (Clockwise)

Preceding gene: 255767816

Following gene: 16080914

Centisome position: 93.87

GC content: 45.97

Gene sequence:

>1092_bases
ATGACTAAACAAACAATTCGCGTTGAATTGACATCAACAAAAAAACCGAAACCAGACCCAAATCAGCTTTCGTTCGGAAG
AGTGTTTACAGACCACATGTTTGTAATGGACTATGCCGCAGATAAAGGTTGGTACGATCCAAGAATCATTCCTTATCAAC
CCTTATCAATGGATCCGGCTGCAATGGTCTATCACTACGGCCAAACCGTGTTTGAAGGGTTAAAGGCTTACGTGTCAGAG
GATGACCATGTTCTGCTTTTCAGACCGGAAAAAAATATGGAACGCCTGAATCAATCAAACGACCGCCTCTGCATCCCGCA
AATTGATGAAGAACAGGTTCTTGAAGGCTTAAAGCAGCTTGTCGCAATTGATAAAGACTGGATTCCAAATGCGGAGGGCA
CGTCCCTTTACATCCGTCCGTTCATCATCGCAACCGAGCCTTTCCTTGGTGTTGCGGCATCTCATACGTATAAGCTCTTG
ATCATTCTTTCTCCGGTCGGCTCTTATTACAAAGAAGGCATTAAGCCGGTCAAAATCGCTGTTGAAAGTGAATTTGTCCG
TGCGGTAAAAGGCGGAACAGGAAATGCCAAAACCGCAGGAAACTATGCTTCAAGCTTAAAAGCGCAGCAGGTAGCCGAAG
AGAAAGGATTTTCTCAAGTACTCTGGCTGGACGGCATTGAGAAGAAATACATCGAAGAAGTCGGAAGCATGAACATCTTC
TTCAAAATCAACGGTGAAATCGTAACACCGATGCTGAACGGGAGCATCCTGGAAGGCATTACGCGCAATTCAGTCATCGC
CTTGCTTAAGCATTGGGGCCTTCAAGTTTCAGAACGAAAAATTGCGATCGATGAGGTCATCCAAGCCCATAAAGACGGCA
TCCTGGAAGAAGCCTTCGGAACAGGTACAGCAGCTGTTATTTCCCCAGTCGGCGAGCTGATCTGGCAGGATGAAACACTT
TCGATCAACAACGGTGAAACAGGAGAAATCGCAAAAAAACTATATGACACGATTACAGGCATTCAAAAAGGCGCTGTCGC
AGACGAATTCGGATGGACGACCGAAGTCGCAGCGCTGACTGAAAGCAAGTAA

Upstream 100 bases:

>100_bases
GCAAACCTATTGCGATTATTTGTTGAAGGTATACAATAGAATATAATTATTTTCAAATAAGTTTGATAATATAAACAATT
TAACAGCAGGGAGATTGACC

Downstream 100 bases:

>100_bases
GAAAAAAGCCGGCCCATTACAGGCCGGCTTTTTTTACGCTTCAATTTTGTTGAAAAACTGCGGCAGATACGCTTTGTGCG
CCTCTAGCATTTCGTCTAAA

Product: branched-chain amino acid aminotransferase

Products: NA

Alternate protein names: BCAT 2; Vegetative protein 85; VEG85

Number of amino acids: Translated: 363; Mature: 362

Protein sequence:

>363_residues
MTKQTIRVELTSTKKPKPDPNQLSFGRVFTDHMFVMDYAADKGWYDPRIIPYQPLSMDPAAMVYHYGQTVFEGLKAYVSE
DDHVLLFRPEKNMERLNQSNDRLCIPQIDEEQVLEGLKQLVAIDKDWIPNAEGTSLYIRPFIIATEPFLGVAASHTYKLL
IILSPVGSYYKEGIKPVKIAVESEFVRAVKGGTGNAKTAGNYASSLKAQQVAEEKGFSQVLWLDGIEKKYIEEVGSMNIF
FKINGEIVTPMLNGSILEGITRNSVIALLKHWGLQVSERKIAIDEVIQAHKDGILEEAFGTGTAAVISPVGELIWQDETL
SINNGETGEIAKKLYDTITGIQKGAVADEFGWTTEVAALTESK

Sequences:

>Translated_363_residues
MTKQTIRVELTSTKKPKPDPNQLSFGRVFTDHMFVMDYAADKGWYDPRIIPYQPLSMDPAAMVYHYGQTVFEGLKAYVSE
DDHVLLFRPEKNMERLNQSNDRLCIPQIDEEQVLEGLKQLVAIDKDWIPNAEGTSLYIRPFIIATEPFLGVAASHTYKLL
IILSPVGSYYKEGIKPVKIAVESEFVRAVKGGTGNAKTAGNYASSLKAQQVAEEKGFSQVLWLDGIEKKYIEEVGSMNIF
FKINGEIVTPMLNGSILEGITRNSVIALLKHWGLQVSERKIAIDEVIQAHKDGILEEAFGTGTAAVISPVGELIWQDETL
SINNGETGEIAKKLYDTITGIQKGAVADEFGWTTEVAALTESK
>Mature_362_residues
TKQTIRVELTSTKKPKPDPNQLSFGRVFTDHMFVMDYAADKGWYDPRIIPYQPLSMDPAAMVYHYGQTVFEGLKAYVSED
DHVLLFRPEKNMERLNQSNDRLCIPQIDEEQVLEGLKQLVAIDKDWIPNAEGTSLYIRPFIIATEPFLGVAASHTYKLLI
ILSPVGSYYKEGIKPVKIAVESEFVRAVKGGTGNAKTAGNYASSLKAQQVAEEKGFSQVLWLDGIEKKYIEEVGSMNIFF
KINGEIVTPMLNGSILEGITRNSVIALLKHWGLQVSERKIAIDEVIQAHKDGILEEAFGTGTAAVISPVGELIWQDETLS
INNGETGEIAKKLYDTITGIQKGAVADEFGWTTEVAALTESK

Specific function: Transaminates branched-chain amino acids and ketoglutarate

COG id: COG0115

COG function: function code EH; Branched-chain amino acid aminotransferase/4-amino-4-deoxychorismate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family

Homologues:

Organism=Homo sapiens, GI296010906, Length=347, Percent_Identity=43.5158501440922, Blast_Score=285, Evalue=5e-77,
Organism=Homo sapiens, GI296010904, Length=347, Percent_Identity=43.5158501440922, Blast_Score=285, Evalue=5e-77,
Organism=Homo sapiens, GI38176287, Length=347, Percent_Identity=43.5158501440922, Blast_Score=285, Evalue=5e-77,
Organism=Homo sapiens, GI50658084, Length=354, Percent_Identity=41.2429378531073, Blast_Score=285, Evalue=6e-77,
Organism=Homo sapiens, GI296010902, Length=361, Percent_Identity=38.5041551246537, Blast_Score=237, Evalue=1e-62,
Organism=Homo sapiens, GI296010900, Length=347, Percent_Identity=39.4812680115274, Blast_Score=237, Evalue=1e-62,
Organism=Homo sapiens, GI258614015, Length=289, Percent_Identity=41.1764705882353, Blast_Score=230, Evalue=1e-60,
Organism=Escherichia coli, GI48994963, Length=321, Percent_Identity=29.2834890965732, Blast_Score=122, Evalue=3e-29,
Organism=Caenorhabditis elegans, GI17568601, Length=361, Percent_Identity=38.2271468144044, Blast_Score=266, Evalue=2e-71,
Organism=Caenorhabditis elegans, GI17565728, Length=320, Percent_Identity=38.125, Blast_Score=213, Evalue=2e-55,
Organism=Saccharomyces cerevisiae, GI6322608, Length=356, Percent_Identity=42.4157303370786, Blast_Score=280, Evalue=2e-76,
Organism=Saccharomyces cerevisiae, GI6322002, Length=370, Percent_Identity=41.6216216216216, Blast_Score=273, Evalue=3e-74,
Organism=Drosophila melanogaster, GI24641779, Length=352, Percent_Identity=42.0454545454545, Blast_Score=261, Evalue=5e-70,

Paralogues:

None

Copy number: 2342 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 11,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): ILVE2_BACSU (P39576)

Other databases:

- EMBL:   Z49992
- EMBL:   AL009126
- EMBL:   X73124
- PIR:   S57763
- RefSeq:   NP_391734.2
- ProteinModelPortal:   P39576
- SMR:   P39576
- EnsemblBacteria:   EBBACT00000002857
- GeneID:   937372
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU38550
- NMPDR:   fig|224308.1.peg.3860
- GenoList:   BSU38550
- GeneTree:   EBGT00070000032338
- HOGENOM:   HBG617123
- PhylomeDB:   P39576
- ProtClustDB:   PRK13357
- BioCyc:   BSUB:BSU38550-MONOMER
- BRENDA:   2.6.1.42
- InterPro:   IPR001544
- InterPro:   IPR018300
- InterPro:   IPR005786
- PANTHER:   PTHR11825
- PANTHER:   PTHR11825:SF2
- PIRSF:   PIRSF006468
- TIGRFAMs:   TIGR01123

Pfam domain/function: PF01063 Aminotran_4; SSF56752 Aminotrans_IV

EC number: =2.6.1.42

Molecular weight: Translated: 40293; Mature: 40161

Theoretical pI: Translated: 4.88; Mature: 4.88

Prosite motif: PS00770 AA_TRANSFER_CLASS_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKQTIRVELTSTKKPKPDPNQLSFGRVFTDHMFVMDYAADKGWYDPRIIPYQPLSMDPA
CCCCEEEEEEECCCCCCCCCCCEEHHHHHHHHEEEEEECCCCCCCCCEECCCCCCCCCHH
AMVYHYGQTVFEGLKAYVSEDDHVLLFRPEKNMERLNQSNDRLCIPQIDEEQVLEGLKQL
HHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHCCCCCEEECCCCCHHHHHHHHHHH
VAIDKDWIPNAEGTSLYIRPFIIATEPFLGVAASHTYKLLIILSPVGSYYKEGIKPVKIA
HHCCCCCCCCCCCCEEEEEEEEEECCCCCCEECCCCEEEEEEECCCHHHHHCCCCEEEEE
VESEFVRAVKGGTGNAKTAGNYASSLKAQQVAEEKGFSQVLWLDGIEKKYIEEVGSMNIF
EHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHCCEEEE
FKINGEIVTPMLNGSILEGITRNSVIALLKHWGLQVSERKIAIDEVIQAHKDGILEEAFG
EEECCEEEEEECCCHHHHCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCHHHHHCC
TGTAAVISPVGELIWQDETLSINNGETGEIAKKLYDTITGIQKGAVADEFGWTTEVAALT
CCHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHHCCCCCHHCCCCHHHHHCC
ESK
CCC
>Mature Secondary Structure 
TKQTIRVELTSTKKPKPDPNQLSFGRVFTDHMFVMDYAADKGWYDPRIIPYQPLSMDPA
CCCEEEEEEECCCCCCCCCCCEEHHHHHHHHEEEEEECCCCCCCCCEECCCCCCCCCHH
AMVYHYGQTVFEGLKAYVSEDDHVLLFRPEKNMERLNQSNDRLCIPQIDEEQVLEGLKQL
HHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHCCCCCEEECCCCCHHHHHHHHHHH
VAIDKDWIPNAEGTSLYIRPFIIATEPFLGVAASHTYKLLIILSPVGSYYKEGIKPVKIA
HHCCCCCCCCCCCCEEEEEEEEEECCCCCCEECCCCEEEEEEECCCHHHHHCCCCEEEEE
VESEFVRAVKGGTGNAKTAGNYASSLKAQQVAEEKGFSQVLWLDGIEKKYIEEVGSMNIF
EHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHCCEEEE
FKINGEIVTPMLNGSILEGITRNSVIALLKHWGLQVSERKIAIDEVIQAHKDGILEEAFG
EEECCEEEEEECCCHHHHCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCHHHHHCC
TGTAAVISPVGELIWQDETLSINNGETGEIAKKLYDTITGIQKGAVADEFGWTTEVAALT
CCHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHHCCCCCHHCCCCHHHHHCC
ESK
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377; 7934828; 9298659