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The map label for this gene is sacP

Identifier: 255767805

GI number: 255767805

Start: 3903646

End: 3905031

Strand: Reverse

Name: sacP

Synonym: BSU38050

Alternate gene names: 255767805

Gene position: 3905031-3903646 (Counterclockwise)

Preceding gene: 16080858

Following gene: 255767804

Centisome position: 92.63

GC content: 47.19

Gene sequence:

>1386_bases
ATGGATTACAAAGAGACTGCAAAACGCCTCATTGAGCTTCTCGGAGGGAAAGAAAATATTATCAGCGCGGCTCATTGTGC
AACAAGACTGCGTTTAGTGATGAAAGATGAATCAAAGATAGATCAAGCACAAGTAGAAGAGCTTGATGGCGTTAAAGGGG
CTTTCAGCAGCTCTGGCCAGTACCAGATCATTTTCGGAACAGGCCTTGTGAATAAAGTATTCGATGCCTTTTCTAAAGAA
GCTGATATCGAGCGTGAAGAACACGTCAATCATCAGGATGCGGCAAAAGAAAAGCTGAATCCCGCTGCGAGATTTGCGAA
AACGCTTTCTAATATTTTTGTTCCAATCATCCCAGCTATTGTAGCCAGCGGCCTATTAATGGGATTGCTGGGTATGATCA
ATGCGTTTCATTGGATGAGCAAGGATTCTGCGCTTTTGCAGCTGCTTGATATGTTTTCAAGTGCAGCATTCATTTTCTTG
CCGATTCTAATCGGGGTCAGCGCTTCAAAAGAGTTTGGCAGCAATCCATACTTGGGAGCGGTCATCGGGGGAATCATGAT
TCATCCGAATCTTTTAAATCCATGGGGATTGGCGGAAGCGACACCTGATTACATGCATCTTTTCGGATTTGATATCGCTC
TTCTCGGCTACCAGGGAACTGTTATCCCTGTCCTGCTTGCGGTGTATGTGATGAGCAAGGTAGAGAAATGGACGAGAAAA
GTGGTTCCACATGCGGTGGATTTGCTTGTTACACCATTTGTTACTGTGATTGTCACCGGGTTTGTTGCTTTTATCGCGAT
TGGCCCTCTAGGCAGAGCGCTTGGCTCCGGCATTACGGTTGCTTTAACTTATGTATATGACCATGCCGGTTTTGTCGCAG
GTCTGATTTTCGGGGGCACGTATTCACTCATCGTGCTGACAGGCGTTCATCACAGCTTCCATGCGATTGAGGCAGGGCTA
ATTGCAGATATAGGCAAAAACTACTTGCTGCCGATCTGGTCAATGGCGAATGTCGCACAGGGCGGGGCAGGTCTTGCCGT
CTTCTTTATGGCGAAAAAGGCAAAGACAAAAGAAATCGCGCTTCCGGCAGCATTTTCCGCTTTTCTCGGCATTACTGAGC
CCGTCATATTCGGAGTCAATCTGCGCTACCGAAAACCGTTTATCGCCGCGATGATCGGAGGCGCATTGGGTGGGGCATAT
GTCGTCTTTACACATGTAGCTGCAAACGCTTACGGGTTAACGGGAATTCCGATGATCGCCATTGCGGCTCCGTTTGGCTT
CAGCAACTTGATTCACTATCTGATTGGCATGGCGATTGCGGCAGTGTCAGCGTTCATTGCAGCATTTGTCATGAAGATTA
ATGAGGATGAGGAGAGGAAAAAATGA

Upstream 100 bases:

>100_bases
GCAAGACCTAAAATTTGCGTAAATGAAAAAGGATCGCTGTGTCCTTTATTCGTTGGCGAATTTTAGGTCTTTTTTGTTTA
AATAAAAGGGGGAGAAATAG

Downstream 100 bases:

>100_bases
CAGCACATGACCAGGAGCTTCGTCGCCGGGCTTATGAAGAAGTGGAGAAAAAAGAGCCCATCGCTAACAGCGATCCGCAC
CGCCAGCATTTTCATATCAT

Product: phosphotransferase system (PTS) sucrose-specific enzyme IIBC component

Products: NA

Alternate protein names: EIIBC-Scr; EII-Scr; Sucrose-specific phosphotransferase enzyme IIB component; PTS system sucrose-specific EIIB component; Sucrose permease IIC component; PTS system sucrose-specific EIIC component

Number of amino acids: Translated: 461; Mature: 461

Protein sequence:

>461_residues
MDYKETAKRLIELLGGKENIISAAHCATRLRLVMKDESKIDQAQVEELDGVKGAFSSSGQYQIIFGTGLVNKVFDAFSKE
ADIEREEHVNHQDAAKEKLNPAARFAKTLSNIFVPIIPAIVASGLLMGLLGMINAFHWMSKDSALLQLLDMFSSAAFIFL
PILIGVSASKEFGSNPYLGAVIGGIMIHPNLLNPWGLAEATPDYMHLFGFDIALLGYQGTVIPVLLAVYVMSKVEKWTRK
VVPHAVDLLVTPFVTVIVTGFVAFIAIGPLGRALGSGITVALTYVYDHAGFVAGLIFGGTYSLIVLTGVHHSFHAIEAGL
IADIGKNYLLPIWSMANVAQGGAGLAVFFMAKKAKTKEIALPAAFSAFLGITEPVIFGVNLRYRKPFIAAMIGGALGGAY
VVFTHVAANAYGLTGIPMIAIAAPFGFSNLIHYLIGMAIAAVSAFIAAFVMKINEDEERKK

Sequences:

>Translated_461_residues
MDYKETAKRLIELLGGKENIISAAHCATRLRLVMKDESKIDQAQVEELDGVKGAFSSSGQYQIIFGTGLVNKVFDAFSKE
ADIEREEHVNHQDAAKEKLNPAARFAKTLSNIFVPIIPAIVASGLLMGLLGMINAFHWMSKDSALLQLLDMFSSAAFIFL
PILIGVSASKEFGSNPYLGAVIGGIMIHPNLLNPWGLAEATPDYMHLFGFDIALLGYQGTVIPVLLAVYVMSKVEKWTRK
VVPHAVDLLVTPFVTVIVTGFVAFIAIGPLGRALGSGITVALTYVYDHAGFVAGLIFGGTYSLIVLTGVHHSFHAIEAGL
IADIGKNYLLPIWSMANVAQGGAGLAVFFMAKKAKTKEIALPAAFSAFLGITEPVIFGVNLRYRKPFIAAMIGGALGGAY
VVFTHVAANAYGLTGIPMIAIAAPFGFSNLIHYLIGMAIAAVSAFIAAFVMKINEDEERKK
>Mature_461_residues
MDYKETAKRLIELLGGKENIISAAHCATRLRLVMKDESKIDQAQVEELDGVKGAFSSSGQYQIIFGTGLVNKVFDAFSKE
ADIEREEHVNHQDAAKEKLNPAARFAKTLSNIFVPIIPAIVASGLLMGLLGMINAFHWMSKDSALLQLLDMFSSAAFIFL
PILIGVSASKEFGSNPYLGAVIGGIMIHPNLLNPWGLAEATPDYMHLFGFDIALLGYQGTVIPVLLAVYVMSKVEKWTRK
VVPHAVDLLVTPFVTVIVTGFVAFIAIGPLGRALGSGITVALTYVYDHAGFVAGLIFGGTYSLIVLTGVHHSFHAIEAGL
IADIGKNYLLPIWSMANVAQGGAGLAVFFMAKKAKTKEIALPAAFSAFLGITEPVIFGVNLRYRKPFIAAMIGGALGGAY
VVFTHVAANAYGLTGIPMIAIAAPFGFSNLIHYLIGMAIAAVSAFIAAFVMKINEDEERKK

Specific function: Acts as both a kinase and a phosphatase on sacT

COG id: COG1263

COG function: function code G; Phosphotransferase system IIC components, glucose/maltose/N-acetylglucosamine-specific

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIC type-1 domain

Homologues:

Organism=Escherichia coli, GI2367362, Length=429, Percent_Identity=33.7995337995338, Blast_Score=259, Evalue=4e-70,
Organism=Escherichia coli, GI1790159, Length=414, Percent_Identity=32.3671497584541, Blast_Score=229, Evalue=3e-61,
Organism=Escherichia coli, GI48994906, Length=457, Percent_Identity=29.3216630196937, Blast_Score=193, Evalue=2e-50,
Organism=Escherichia coli, GI1788769, Length=410, Percent_Identity=30.4878048780488, Blast_Score=172, Evalue=6e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PTSBC_BACSU (P05306)

Other databases:

- EMBL:   J03006
- EMBL:   X73124
- EMBL:   AL009126
- PIR:   A39938
- RefSeq:   NP_391684.3
- ProteinModelPortal:   P05306
- SMR:   P05306
- EnsemblBacteria:   EBBACT00000003833
- GeneID:   937266
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU38050
- GenoList:   BSU38050
- GeneTree:   EBGT00050000000033
- HOGENOM:   HBG673521
- PhylomeDB:   P05306
- ProtClustDB:   CLSK873148
- BioCyc:   BSUB:BSU38050-MONOMER
- BRENDA:   2.7.1.69
- InterPro:   IPR018113
- InterPro:   IPR004719
- InterPro:   IPR001996
- InterPro:   IPR003352
- InterPro:   IPR013013
- InterPro:   IPR011535
- InterPro:   IPR010973
- Gene3D:   G3DSA:3.30.1360.60
- TIGRFAMs:   TIGR00826
- TIGRFAMs:   TIGR00852
- TIGRFAMs:   TIGR01996

Pfam domain/function: PF00367 PTS_EIIB; PF02378 PTS_EIIC; SSF55604 PTS_EIIB

EC number: =2.7.1.69

Molecular weight: Translated: 49398; Mature: 49398

Theoretical pI: Translated: 7.91; Mature: 7.91

Prosite motif: PS51098 PTS_EIIB_TYPE_1; PS01035 PTS_EIIB_TYPE_1_CYS; PS51103 PTS_EIIC_TYPE_1; PS00134 TRYPSIN_HIS

Important sites: ACT_SITE 26-26

Signals:

None

Transmembrane regions:

HASH(0x12fde820)-; HASH(0x13006130)-; HASH(0x11a2d5e0)-; HASH(0x131c5a24)-; HASH(0x12ccb8c0)-; HASH(0x12039bb8)-; HASH(0x1100bf28)-; HASH(0x12a31204)-; HASH(0x10b04834)-; HASH(0x1318c624)-;

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDYKETAKRLIELLGGKENIISAAHCATRLRLVMKDESKIDQAQVEELDGVKGAFSSSGQ
CCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCC
YQIIFGTGLVNKVFDAFSKEADIEREEHVNHQDAAKEKLNPAARFAKTLSNIFVPIIPAI
EEEEEECCHHHHHHHHHHHHCCCCHHHCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
VASGLLMGLLGMINAFHWMSKDSALLQLLDMFSSAAFIFLPILIGVSASKEFGSNPYLGA
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHCCCCCCHHH
VIGGIMIHPNLLNPWGLAEATPDYMHLFGFDIALLGYQGTVIPVLLAVYVMSKVEKWTRK
HHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHEECCCCHHHHHHHHHHHHHHHHHHHHH
VVPHAVDLLVTPFVTVIVTGFVAFIAIGPLGRALGSGITVALTYVYDHAGFVAGLIFGGT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHCCC
YSLIVLTGVHHSFHAIEAGLIADIGKNYLLPIWSMANVAQGGAGLAVFFMAKKAKTKEIA
EEEEEEECCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCEEEEEEHHHCCCHHHC
LPAAFSAFLGITEPVIFGVNLRYRKPFIAAMIGGALGGAYVVFTHVAANAYGLTGIPMIA
CHHHHHHHHCCCCHHEECCCEEECCHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCHHH
IAAPFGFSNLIHYLIGMAIAAVSAFIAAFVMKINEDEERKK
HCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCH
>Mature Secondary Structure
MDYKETAKRLIELLGGKENIISAAHCATRLRLVMKDESKIDQAQVEELDGVKGAFSSSGQ
CCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCC
YQIIFGTGLVNKVFDAFSKEADIEREEHVNHQDAAKEKLNPAARFAKTLSNIFVPIIPAI
EEEEEECCHHHHHHHHHHHHCCCCHHHCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
VASGLLMGLLGMINAFHWMSKDSALLQLLDMFSSAAFIFLPILIGVSASKEFGSNPYLGA
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHCCCCCCHHH
VIGGIMIHPNLLNPWGLAEATPDYMHLFGFDIALLGYQGTVIPVLLAVYVMSKVEKWTRK
HHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHEECCCCHHHHHHHHHHHHHHHHHHHHH
VVPHAVDLLVTPFVTVIVTGFVAFIAIGPLGRALGSGITVALTYVYDHAGFVAGLIFGGT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHCCC
YSLIVLTGVHHSFHAIEAGLIADIGKNYLLPIWSMANVAQGGAGLAVFFMAKKAKTKEIA
EEEEEEECCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCEEEEEEHHHCCCHHHC
LPAAFSAFLGITEPVIFGVNLRYRKPFIAAMIGGALGGAYVVFTHVAANAYGLTGIPMIA
CHHHHHHHHCCCCHHEECCCEEECCHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCHHH
IAAPFGFSNLIHYLIGMAIAAVSAFIAAFVMKINEDEERKK
HCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 3122206; 7934828; 9384377; 10568751