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The map label for this gene is sacA

Identifier: 255767804

GI number: 255767804

Start: 3902210

End: 3903649

Strand: Reverse

Name: sacA

Synonym: BSU38040

Alternate gene names: 255767804

Gene position: 3903649-3902210 (Counterclockwise)

Preceding gene: 255767805

Following gene: 16080854

Centisome position: 92.6

GC content: 48.82

Gene sequence:

>1440_bases
ATGACAGCACATGACCAGGAGCTTCGTCGCCGGGCTTATGAAGAAGTGGAGAAAAAAGAGCCCATCGCTAACAGCGATCC
GCACCGCCAGCATTTTCATATCATGCCGCCGGTTGGGCTGCTGAATGACCCGAATGGCGTGATTTATTGGAAGGGCAGCT
ATCATGTATTCTTTCAGTGGCAGCCGTTTCAGACGGGGCACGGCGCAAAATTTTGGGGGCATTATACGACACAGGATGTT
GTGAATTGGAAGCGGGAAGAGATTGCGCTGGCTCCGAGTGATTGGTTTGATAAAAACGGCTGCTACTCGGGCAGCGCTGT
CACGAAAGACGATCGGCTCTATCTTTTTTACACAGGAAATGTCAGGGATCAGGATGGAAATCGGGAAACGTATCAATGCC
TTGCTGTTTCTGACGACGGGCTGTCCTTTGAGAAAAAGGGTGTCGTCGCCCGCCTTCCGGAAGGATATACGGCGCATTTT
CGCGATCCGAAGGTATGGGAGCATGAAGGCACATGGTATATGGTGATTGGTGCGCAAACAGAGAATTTGAAAGGGCAGGC
TGTGTTGTTTGCTTCTGATAACCTGACAGAGTGGAGATTTCTTGGCCCGATAACCGGCGCGGGCTTCAACGGGCTGGACG
ATTTTGGATACATGTGGGAATGCCCTGATTTGTTTTCCCTTCAAGGATCGGATGTGCTGATTGTTTCGCCTCAAGGGCTT
GAGGCTGACGGTTTCCGTTATCAGAACGTATATCAATCAGGTTATTTTGTCGGCCGCCTCGATTATAACAAGCCTGAACT
GAAGCATGGTGAATTTACGGAGCTTGATCAAGGTTTTGATTTTTACGCGCCGCAAACACTTGAAGACGATCAGGGAAGGC
GGATTTTATTTGCATGGATGGCGGTGCCTGATCAGGATGAAGGGTCCCATCCGACCATTGACTGCCACTGGATTCACTGC
ATGACGCTGCCGAGACAGCTGACGCTTTCAGGACAGAAGCTGATTCAGCAGCCGCTGCCTGAGCTAAAAGCCATGCGCAG
AAATGAGAAAAAAATACACATCAACATGCATGGATCATCTGGTGCGCTTCCAGTGGAAAAACCTGAAAGAACTGAGATTC
TACTGGAAGACATTCATACGGAGTCTGGCTTTTCAATCAGTATCCGCGGAACGGCTACGTTTTCCTTCCATAAAGACGAG
GGGATTGTTACGCTGGAACGAAAGAGCTTTGACGGAAAAAGAACAGAAGCGAGACATTGCCGCATCAAGGATTTGCATAC
CGTACACATGTTTCTCGACGCGTCATCTGTGGAAATCTTTATCAATAACGGAGAAGAGGTCTTGAGTGCAAGATATTTTC
CTTTCCCGGGAAATCATGAAGTAACAGCCAGTGCGACCGGGAAATCTGAAATGAATGTCGGAATTTGGACACTTATGTAG

Upstream 100 bases:

>100_bases
GCAACTTGATTCACTATCTGATTGGCATGGCGATTGCGGCAGTGTCAGCGTTCATTGCAGCATTTGTCATGAAGATTAAT
GAGGATGAGGAGAGGAAAAA

Downstream 100 bases:

>100_bases
AAAATCCTTCTATTTTCTTATGTGAAATCTGAGCCATCTGTGCTATTCTTCAACGATAACCTACTATTTTTTTCTGTAAA
AGGAGTTGTTTTCTTGAATA

Product: sucrase-6-phosphate hydrolase

Products: NA

Alternate protein names: Sucrase; Invertase

Number of amino acids: Translated: 479; Mature: 478

Protein sequence:

>479_residues
MTAHDQELRRRAYEEVEKKEPIANSDPHRQHFHIMPPVGLLNDPNGVIYWKGSYHVFFQWQPFQTGHGAKFWGHYTTQDV
VNWKREEIALAPSDWFDKNGCYSGSAVTKDDRLYLFYTGNVRDQDGNRETYQCLAVSDDGLSFEKKGVVARLPEGYTAHF
RDPKVWEHEGTWYMVIGAQTENLKGQAVLFASDNLTEWRFLGPITGAGFNGLDDFGYMWECPDLFSLQGSDVLIVSPQGL
EADGFRYQNVYQSGYFVGRLDYNKPELKHGEFTELDQGFDFYAPQTLEDDQGRRILFAWMAVPDQDEGSHPTIDCHWIHC
MTLPRQLTLSGQKLIQQPLPELKAMRRNEKKIHINMHGSSGALPVEKPERTEILLEDIHTESGFSISIRGTATFSFHKDE
GIVTLERKSFDGKRTEARHCRIKDLHTVHMFLDASSVEIFINNGEEVLSARYFPFPGNHEVTASATGKSEMNVGIWTLM

Sequences:

>Translated_479_residues
MTAHDQELRRRAYEEVEKKEPIANSDPHRQHFHIMPPVGLLNDPNGVIYWKGSYHVFFQWQPFQTGHGAKFWGHYTTQDV
VNWKREEIALAPSDWFDKNGCYSGSAVTKDDRLYLFYTGNVRDQDGNRETYQCLAVSDDGLSFEKKGVVARLPEGYTAHF
RDPKVWEHEGTWYMVIGAQTENLKGQAVLFASDNLTEWRFLGPITGAGFNGLDDFGYMWECPDLFSLQGSDVLIVSPQGL
EADGFRYQNVYQSGYFVGRLDYNKPELKHGEFTELDQGFDFYAPQTLEDDQGRRILFAWMAVPDQDEGSHPTIDCHWIHC
MTLPRQLTLSGQKLIQQPLPELKAMRRNEKKIHINMHGSSGALPVEKPERTEILLEDIHTESGFSISIRGTATFSFHKDE
GIVTLERKSFDGKRTEARHCRIKDLHTVHMFLDASSVEIFINNGEEVLSARYFPFPGNHEVTASATGKSEMNVGIWTLM
>Mature_478_residues
TAHDQELRRRAYEEVEKKEPIANSDPHRQHFHIMPPVGLLNDPNGVIYWKGSYHVFFQWQPFQTGHGAKFWGHYTTQDVV
NWKREEIALAPSDWFDKNGCYSGSAVTKDDRLYLFYTGNVRDQDGNRETYQCLAVSDDGLSFEKKGVVARLPEGYTAHFR
DPKVWEHEGTWYMVIGAQTENLKGQAVLFASDNLTEWRFLGPITGAGFNGLDDFGYMWECPDLFSLQGSDVLIVSPQGLE
ADGFRYQNVYQSGYFVGRLDYNKPELKHGEFTELDQGFDFYAPQTLEDDQGRRILFAWMAVPDQDEGSHPTIDCHWIHCM
TLPRQLTLSGQKLIQQPLPELKAMRRNEKKIHINMHGSSGALPVEKPERTEILLEDIHTESGFSISIRGTATFSFHKDEG
IVTLERKSFDGKRTEARHCRIKDLHTVHMFLDASSVEIFINNGEEVLSARYFPFPGNHEVTASATGKSEMNVGIWTLM

Specific function: Unknown

COG id: COG1621

COG function: function code G; Beta-fructosidases (levanase/invertase)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 32 family

Homologues:

Organism=Saccharomyces cerevisiae, GI6322029, Length=533, Percent_Identity=24.390243902439, Blast_Score=102, Evalue=2e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SCRB_BACSU (P07819)

Other databases:

- EMBL:   M15662
- EMBL:   X73124
- EMBL:   AL009126
- EMBL:   J03006
- PIR:   A25562
- RefSeq:   NP_391683.2
- ProteinModelPortal:   P07819
- SMR:   P07819
- EnsemblBacteria:   EBBACT00000000184
- GeneID:   937277
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU38040
- NMPDR:   fig|224308.1.peg.3809
- GenoList:   BSU38040
- GeneTree:   EBGT00050000001979
- HOGENOM:   HBG693902
- PhylomeDB:   P07819
- ProtClustDB:   CLSK873150
- BioCyc:   BSUB:BSU38040-MONOMER
- BRENDA:   3.2.1.26
- GO:   GO:0005737
- InterPro:   IPR008985
- InterPro:   IPR001362
- InterPro:   IPR018053
- InterPro:   IPR013189
- InterPro:   IPR013148
- InterPro:   IPR006232
- SMART:   SM00640
- TIGRFAMs:   TIGR01322

Pfam domain/function: PF08244 Glyco_hydro_32C; PF00251 Glyco_hydro_32N; SSF49899 ConA_like_lec_gl

EC number: =3.2.1.26

Molecular weight: Translated: 54815; Mature: 54684

Theoretical pI: Translated: 5.64; Mature: 5.64

Prosite motif: PS00609 GLYCOSYL_HYDROL_F32

Important sites: ACT_SITE 43-43 BINDING 59-59 BINDING 220-220

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAHDQELRRRAYEEVEKKEPIANSDPHRQHFHIMPPVGLLNDPNGVIYWKGSYHVFFQW
CCCCHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCCCCCCCEEEEECCCEEEEEEE
QPFQTGHGAKFWGHYTTQDVVNWKREEIALAPSDWFDKNGCYSGSAVTKDDRLYLFYTGN
ECCCCCCCCEEECCCCCHHHHCCCCCEEEECCCCCCCCCCCCCCCEECCCCEEEEEEECC
VRDQDGNRETYQCLAVSDDGLSFEKKGVVARLPEGYTAHFRDPKVWEHEGTWYMVIGAQT
EECCCCCCCEEEEEEECCCCCCCCCCCCEEECCCCCEEECCCCCEEECCCCEEEEEECCC
ENLKGQAVLFASDNLTEWRFLGPITGAGFNGLDDFGYMWECPDLFSLQGSDVLIVSPQGL
CCCCCCEEEEECCCCCCEEEECCCCCCCCCCCCCCCCEECCCCCEEECCCCEEEECCCCC
EADGFRYQNVYQSGYFVGRLDYNKPELKHGEFTELDQGFDFYAPQTLEDDQGRRILFAWM
CCCCEEEEEEHHCCEEEEEECCCCCCCCCCCCCHHCCCCCCCCCCCCCCCCCCEEEEEEE
AVPDQDEGSHPTIDCHWIHCMTLPRQLTLSGQKLIQQPLPELKAMRRNEKKIHINMHGSS
ECCCCCCCCCCEEEEEEEEEECCCCEEECCHHHHHHCCCHHHHHHHCCCCEEEEEECCCC
GALPVEKPERTEILLEDIHTESGFSISIRGTATFSFHKDEGIVTLERKSFDGKRTEARHC
CCCCCCCCCHHHHHEEHHCCCCCCEEEEEEEEEEEEECCCCEEEEEECCCCCCCCCCCCC
RIKDLHTVHMFLDASSVEIFINNGEEVLSARYFPFPGNHEVTASATGKSEMNVGIWTLM
EEECEEEEEEEEECCEEEEEECCCHHHHEEECCCCCCCCEEEEECCCCCCCEEEEEEEC
>Mature Secondary Structure 
TAHDQELRRRAYEEVEKKEPIANSDPHRQHFHIMPPVGLLNDPNGVIYWKGSYHVFFQW
CCCHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCCCCCCCEEEEECCCEEEEEEE
QPFQTGHGAKFWGHYTTQDVVNWKREEIALAPSDWFDKNGCYSGSAVTKDDRLYLFYTGN
ECCCCCCCCEEECCCCCHHHHCCCCCEEEECCCCCCCCCCCCCCCEECCCCEEEEEEECC
VRDQDGNRETYQCLAVSDDGLSFEKKGVVARLPEGYTAHFRDPKVWEHEGTWYMVIGAQT
EECCCCCCCEEEEEEECCCCCCCCCCCCEEECCCCCEEECCCCCEEECCCCEEEEEECCC
ENLKGQAVLFASDNLTEWRFLGPITGAGFNGLDDFGYMWECPDLFSLQGSDVLIVSPQGL
CCCCCCEEEEECCCCCCEEEECCCCCCCCCCCCCCCCEECCCCCEEECCCCEEEECCCCC
EADGFRYQNVYQSGYFVGRLDYNKPELKHGEFTELDQGFDFYAPQTLEDDQGRRILFAWM
CCCCEEEEEEHHCCEEEEEECCCCCCCCCCCCCHHCCCCCCCCCCCCCCCCCCEEEEEEE
AVPDQDEGSHPTIDCHWIHCMTLPRQLTLSGQKLIQQPLPELKAMRRNEKKIHINMHGSS
ECCCCCCCCCCEEEEEEEEEECCCCEEECCHHHHHHCCCHHHHHHHCCCCEEEEEECCCC
GALPVEKPERTEILLEDIHTESGFSISIRGTATFSFHKDEGIVTLERKSFDGKRTEARHC
CCCCCCCCCHHHHHEEHHCCCCCCEEEEEEEEEEEEECCCCEEEEEECCCCCCCCCCCCC
RIKDLHTVHMFLDASSVEIFINNGEEVLSARYFPFPGNHEVTASATGKSEMNVGIWTLM
EEECEEEEEEEEECCEEEEEECCCHHHHEEECCCCCCCCEEEEECCCCCCCEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3100393; 7934828; 9384377; 3122206