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The map label for this gene is spsK

Identifier: 255767795

GI number: 255767795

Start: 3883427

End: 3884278

Strand: Reverse

Name: spsK

Synonym: BSU37820

Alternate gene names: 255767795

Gene position: 3884278-3883427 (Counterclockwise)

Preceding gene: 16080834

Following gene: 50812301

Centisome position: 92.14

GC content: 49.77

Gene sequence:

>852_bases
TTGACAAAGGTATTGGTGACTGGGGCGGGAGGACAGCTTGGCTTGGAACTATGCCGCCAGCTGAAGCAAGCCGGATATGA
GGTCATTGCGTTAACAAAGAAGATGATGAACATTGCTGACCAGCGCTCAGTACGGCATTCGTTTGGCCATTATCAGCCGG
ATATCGTGGTCAATAGCGCCGCGTTTACGTCGGTTGATCAATGCGAGAAAGAGCTTGATAAAGCGTACTTGATTAACGGA
ATCGGCGCTTATTATACAGCGCTTGAGTCAACCCGCATCGGAGCTCAATATGTTCATATCAGCACGGACTATGTATTCAA
TGGGAAAGGAACCCAGCCATATAGGGAAGACGATCCCCTCGATCCGAAAACCATTTATGGCAAAAGCAAAAGGCTCGGAG
AAGAACTGATACGATTAACGACAAAAGACAGCACGATCATCAGGACATCGTGGGTGTACGGACATGGCGGCAGTAATTTC
GTTGAAACCATGCTGAAACTTGCTGAAACGAAACAAGAGCTTCGGGTTGTCAGCGATCAAATTGGTTCGCCGACTTATAC
GAAGGATTTAGCAGAAGCGGTCATCAAGCTGTTCAGCCATCCGCCGGGCATCTATCATGTCAGCAATTCAGGCATATGCA
GCTGGTATGAGTTTGCGACGGCAATTATGGAAGAAAGCGGGCTGGAAACAGCCATTTTGTCTGTAACGACAGAGGAATAC
GGGAACAAAACGCCCCGTCCGGCATACTCTGTGCTGAGCCATCGGGCTATTGAAGAAGCCGGCATCCGCCCCCGCCACTG
GCGGGAAGCGTTACGAGAGTATTTGCAGGAAAGGAGCAGTGCGTGTGATTGA

Upstream 100 bases:

>100_bases
AAAAACGAACTGGGCTGGCGGCAGGAAGTGACGTTTGAAGAAGGCATTGCGCGTACGATTCAGTGGTATACAGACAATGA
CCGATGAGAAGGTGATCTGA

Downstream 100 bases:

>100_bases
TGGAGTCAAGGTGAAAAAATTGATGAAGCATAGCGATGACAGAGGCTTTTTTGCTGAGCTGGTCCGGGACGATGAGAATC
TCCTTGAGCATTTTGGGCAG

Product: dTDP-4-dehydrorhamnose reductase

Products: dTDP-4-dehydro-6-deoxy-L-mannose; NADPH; H+

Alternate protein names: NA

Number of amino acids: Translated: 283; Mature: 282

Protein sequence:

>283_residues
MTKVLVTGAGGQLGLELCRQLKQAGYEVIALTKKMMNIADQRSVRHSFGHYQPDIVVNSAAFTSVDQCEKELDKAYLING
IGAYYTALESTRIGAQYVHISTDYVFNGKGTQPYREDDPLDPKTIYGKSKRLGEELIRLTTKDSTIIRTSWVYGHGGSNF
VETMLKLAETKQELRVVSDQIGSPTYTKDLAEAVIKLFSHPPGIYHVSNSGICSWYEFATAIMEESGLETAILSVTTEEY
GNKTPRPAYSVLSHRAIEEAGIRPRHWREALREYLQERSSACD

Sequences:

>Translated_283_residues
MTKVLVTGAGGQLGLELCRQLKQAGYEVIALTKKMMNIADQRSVRHSFGHYQPDIVVNSAAFTSVDQCEKELDKAYLING
IGAYYTALESTRIGAQYVHISTDYVFNGKGTQPYREDDPLDPKTIYGKSKRLGEELIRLTTKDSTIIRTSWVYGHGGSNF
VETMLKLAETKQELRVVSDQIGSPTYTKDLAEAVIKLFSHPPGIYHVSNSGICSWYEFATAIMEESGLETAILSVTTEEY
GNKTPRPAYSVLSHRAIEEAGIRPRHWREALREYLQERSSACD
>Mature_282_residues
TKVLVTGAGGQLGLELCRQLKQAGYEVIALTKKMMNIADQRSVRHSFGHYQPDIVVNSAAFTSVDQCEKELDKAYLINGI
GAYYTALESTRIGAQYVHISTDYVFNGKGTQPYREDDPLDPKTIYGKSKRLGEELIRLTTKDSTIIRTSWVYGHGGSNFV
ETMLKLAETKQELRVVSDQIGSPTYTKDLAEAVIKLFSHPPGIYHVSNSGICSWYEFATAIMEESGLETAILSVTTEEYG
NKTPRPAYSVLSHRAIEEAGIRPRHWREALREYLQERSSACD

Specific function: DTDP-L-RHAMNOSE BIOSYNTHESIS WITHIN THE O ANTIGEN BIOSYNTHESIS PATHWAY OF LIPOPOLYSACCHARIDE BIOSYNTHESIS. [C]

COG id: COG1091

COG function: function code M; dTDP-4-dehydrorhamnose reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dTDP-4-dehydrorhamnose reductase family

Homologues:

Organism=Homo sapiens, GI33519455, Length=291, Percent_Identity=28.1786941580756, Blast_Score=96, Evalue=5e-20,
Organism=Homo sapiens, GI11034825, Length=291, Percent_Identity=28.1786941580756, Blast_Score=96, Evalue=5e-20,
Organism=Escherichia coli, GI1788352, Length=293, Percent_Identity=34.8122866894198, Blast_Score=161, Evalue=6e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SPSK_BACSU (P39631)

Other databases:

- EMBL:   X73124
- EMBL:   AL009126
- PIR:   S39728
- RefSeq:   NP_391661.3
- ProteinModelPortal:   P39631
- SMR:   P39631
- EnsemblBacteria:   EBBACT00000002329
- GeneID:   937225
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU37820
- GenoList:   BSU37820
- GeneTree:   EBGT00050000000677
- HOGENOM:   HBG541114
- ProtClustDB:   CLSK928155
- BioCyc:   BSUB:BSU37820-MONOMER
- GO:   GO:0005488
- InterPro:   IPR005913
- InterPro:   IPR016040
- Gene3D:   G3DSA:3.40.50.720
- TIGRFAMs:   TIGR01214

Pfam domain/function: PF04321 RmlD_sub_bind

EC number: 1.1.1.133

Molecular weight: Translated: 31642; Mature: 31511

Theoretical pI: Translated: 6.72; Mature: 6.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKVLVTGAGGQLGLELCRQLKQAGYEVIALTKKMMNIADQRSVRHSFGHYQPDIVVNSA
CCEEEEECCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCH
AFTSVDQCEKELDKAYLINGIGAYYTALESTRIGAQYVHISTDYVFNGKGTQPYREDDPL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECEEECCCCCCCCCCCCCC
DPKTIYGKSKRLGEELIRLTTKDSTIIRTSWVYGHGGSNFVETMLKLAETKQELRVVSDQ
CCHHHCCCHHHHHHHHHHHHCCCCEEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHH
IGSPTYTKDLAEAVIKLFSHPPGIYHVSNSGICSWYEFATAIMEESGLETAILSVTTEEY
CCCCCHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHCCCCEEHHEEEHHHH
GNKTPRPAYSVLSHRAIEEAGIRPRHWREALREYLQERSSACD
CCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TKVLVTGAGGQLGLELCRQLKQAGYEVIALTKKMMNIADQRSVRHSFGHYQPDIVVNSA
CEEEEECCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCH
AFTSVDQCEKELDKAYLINGIGAYYTALESTRIGAQYVHISTDYVFNGKGTQPYREDDPL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECEEECCCCCCCCCCCCCC
DPKTIYGKSKRLGEELIRLTTKDSTIIRTSWVYGHGGSNFVETMLKLAETKQELRVVSDQ
CCHHHCCCHHHHHHHHHHHHCCCCEEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHH
IGSPTYTKDLAEAVIKLFSHPPGIYHVSNSGICSWYEFATAIMEESGLETAILSVTTEEY
CCCCCHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHCCCCEEHHEEEHHHH
GNKTPRPAYSVLSHRAIEEAGIRPRHWREALREYLQERSSACD
CCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NADPH [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: dTDP-6-deoxy-L-mannose; NADP+

Specific reaction: dTDP-6-deoxy-L-mannose + NADP+ = dTDP-4-dehydro-6-deoxy-L-mannose + NADPH + H+

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7934828; 9384377; 10568751