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The map label for this gene is spsL

Identifier: 50812301

GI number: 50812301

Start: 3882979

End: 3883434

Strand: Reverse

Name: spsL

Synonym: BSU37810

Alternate gene names: 50812301

Gene position: 3883434-3882979 (Counterclockwise)

Preceding gene: 255767795

Following gene: 16080832

Centisome position: 92.12

GC content: 45.83

Gene sequence:

>456_bases
GTGATTGATGGAGTCAAGGTGAAAAAATTGATGAAGCATAGCGATGACAGAGGCTTTTTTGCTGAGCTGGTCCGGGACGA
TGAGAATCTCCTTGAGCATTTTGGGCAGGCCTCTTGGTCGAAAAGCTATCCGGGTGTGATTAAGGCATTTCATTATCATG
AGAAGCAGGATGATCTCTGGTTCTTCCCAACCGGCCACGCTCAGGTTGTCCTATACGATCTTCGAGAGGATTCAAAGACA
AAAGGAGAAACGGACGTCTATTATATGGGCGAAGACAACCCGATGCTGTTATTAATCCCAAAAGGGGTGGCTCACGGATA
TAGAGTGCTGGGAGAGACGCCGCTGACAATTATCTATTTTACCACCATGTCGTATAACCCGGATCAGCCTGATGAAAAAA
GAATTCCGTGGGATGATGAGACGATCGGTTTTAACTGGAACACTGAATTCAGATAA

Upstream 100 bases:

>100_bases
TACTCTGTGCTGAGCCATCGGGCTATTGAAGAAGCCGGCATCCGCCCCCGCCACTGGCGGGAAGCGTTACGAGAGTATTT
GCAGGAAAGGAGCAGTGCGT

Downstream 100 bases:

>100_bases
AAATAGGAACGTGATCAATCGAAGAGCAGAGGCATCTTCGACTTTTTTTGCCCTTTTTTAAAATAGGATAAAAGATATAA
AATGTCAGGCAGATGTTACT

Product: nucleotide sugar epimerase

Products: dTDP-4-dehydro-6-deoxy-L-mannose

Alternate protein names: NA

Number of amino acids: Translated: 151; Mature: 151

Protein sequence:

>151_residues
MIDGVKVKKLMKHSDDRGFFAELVRDDENLLEHFGQASWSKSYPGVIKAFHYHEKQDDLWFFPTGHAQVVLYDLREDSKT
KGETDVYYMGEDNPMLLLIPKGVAHGYRVLGETPLTIIYFTTMSYNPDQPDEKRIPWDDETIGFNWNTEFR

Sequences:

>Translated_151_residues
MIDGVKVKKLMKHSDDRGFFAELVRDDENLLEHFGQASWSKSYPGVIKAFHYHEKQDDLWFFPTGHAQVVLYDLREDSKT
KGETDVYYMGEDNPMLLLIPKGVAHGYRVLGETPLTIIYFTTMSYNPDQPDEKRIPWDDETIGFNWNTEFR
>Mature_151_residues
MIDGVKVKKLMKHSDDRGFFAELVRDDENLLEHFGQASWSKSYPGVIKAFHYHEKQDDLWFFPTGHAQVVLYDLREDSKT
KGETDVYYMGEDNPMLLLIPKGVAHGYRVLGETPLTIIYFTTMSYNPDQPDEKRIPWDDETIGFNWNTEFR

Specific function: DTDP-L-RHAMNOSE BIOSYNTHESIS WITHIN THE O ANTIGEN BIOSYNTHESIS PATHWAY OF LIPOPOLYSACCHARIDE BIOSYNTHESIS. [C]

COG id: COG1898

COG function: function code M; dTDP-4-dehydrorhamnose 3,5-epimerase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: To dTDP-4-dehydrorhamnose reductase

Homologues:

Organism=Escherichia coli, GI1788350, Length=142, Percent_Identity=33.0985915492958, Blast_Score=60, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SPSL_BACSU (Q7WY56)

Other databases:

- EMBL:   AL009126
- RefSeq:   YP_054595.1
- ProteinModelPortal:   Q7WY56
- SMR:   Q7WY56
- EnsemblBacteria:   EBBACT00000003966
- GeneID:   2914201
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU37810
- GenoList:   BSU37810
- GeneTree:   EBGT00050000001957
- HOGENOM:   HBG730537
- OMA:   VIPDERG
- ProtClustDB:   CLSK873589
- BioCyc:   BSUB:BSU37810-MONOMER
- InterPro:   IPR011051
- InterPro:   IPR000888
- InterPro:   IPR014710
- Gene3D:   G3DSA:2.60.120.10
- PANTHER:   PTHR21047
- ProDom:   PD001462

Pfam domain/function: PF00908 dTDP_sugar_isom; SSF51182 RmlC_like_cupin

EC number: 5.1.3.13

Molecular weight: Translated: 17683; Mature: 17683

Theoretical pI: Translated: 4.86; Mature: 4.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDGVKVKKLMKHSDDRGFFAELVRDDENLLEHFGQASWSKSYPGVIKAFHYHEKQDDLW
CCCCHHHHHHHHCCCCCCHHHHHHCCHHHHHHHHCCCCCCCCCCCHHHHEECCCCCCCEE
FFPTGHAQVVLYDLREDSKTKGETDVYYMGEDNPMLLLIPKGVAHGYRVLGETPLTIIYF
EEECCCCEEEEEECCCCCCCCCCCEEEEECCCCCEEEEEECCCCCCEEEECCCCEEEEEE
TTMSYNPDQPDEKRIPWDDETIGFNWNTEFR
EEECCCCCCCCCCCCCCCCCEECCCCCCCCC
>Mature Secondary Structure
MIDGVKVKKLMKHSDDRGFFAELVRDDENLLEHFGQASWSKSYPGVIKAFHYHEKQDDLW
CCCCHHHHHHHHCCCCCCHHHHHHCCHHHHHHHHCCCCCCCCCCCHHHHEECCCCCCCEE
FFPTGHAQVVLYDLREDSKTKGETDVYYMGEDNPMLLLIPKGVAHGYRVLGETPLTIIYF
EEECCCCEEEEEECCCCCCCCCCCEEEEECCCCCEEEEEECCCCCCEEEECCCCEEEEEE
TTMSYNPDQPDEKRIPWDDETIGFNWNTEFR
EEECCCCCCCCCCCCCCCCCEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.025 {dTDP-4-dehydro-6-deoxy-D-glucose}} [C]

Substrates: dTDP-4-dehydro-6-deoxy-D-glucose

Specific reaction: dTDP-4-dehydro-6-deoxy-D-glucose = dTDP-4-dehydro-6-deoxy-L-mannose

General reaction: Epimerization [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377