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The map label for this gene is dnaG

Identifier: 255767574

GI number: 255767574

Start: 2601528

End: 2603339

Strand: Reverse

Name: dnaG

Synonym: BSU25210

Alternate gene names: 255767574

Gene position: 2603339-2601528 (Counterclockwise)

Preceding gene: 255767575

Following gene: 255767573

Centisome position: 61.75

GC content: 43.32

Gene sequence:

>1812_bases
ATGGGAAATCGGATACCAGATGAAATTGTGGATCAGGTGCAAAAGTCGGCAGATATCGTTGAAGTCATAGGTGATTATGT
TCAATTAAAGAAGCAAGGCCGAAACTACTTTGGACTCTGTCCTTTTCATGGAGAAAGCACACCTTCGTTTTCCGTATCGC
CCGACAAACAGATTTTTCATTGCTTTGGCTGCGGAGCGGGCGGCAATGTTTTCTCTTTTTTAAGGCAGATGGAAGGCTAT
TCTTTTGCCGAGTCGGTTTCTCACCTTGCTGACAAATACCAAATTGATTTTCCAGATGATATAACAGTCCATTCCGGAGC
CCGGCCAGAGTCTTCTGGAGAACAAAAAATGGCTGAGGCACATGAGCTCCTGAAGAAATTTTACCATCATTTGTTAATAA
ATACAAAAGAAGGTCAAGAGGCACTGGATTATCTGCTTTCTAGGGGCTTTACGAAAGAGCTGATTAATGAATTTCAGATT
GGCTATGCTCTTGATTCTTGGGACTTTATCACGAAATTCCTTGTAAAGAGGGGATTTAGTGAGGCGCAAATGGAAAAAGC
GGGTCTCCTGATCAGACGCGAAGACGGAAGCGGATATTTCGACCGCTTCAGAAACCGTGTCATGTTTCCGATCCATGATC
ATCACGGGGCTGTTGTTGCTTTCTCAGGCAGGGCTCTTGGCAGCCAGCAGCCTAAGTATATGAACAGTCCTGAAACCCCG
CTCTTTCATAAAAGCAAACTGCTTTACAATTTTTATAAGGCCCGCCTTCATATCAGAAAGCAGGAAAGAGCAGTCTTATT
TGAAGGGTTTGCTGATGTCATATCGGCCGTAAGCTCGGATGTAAAGGAAAGCATAGCCACGATGGGAACGTCTCTTACAG
ATGATCATGTCAAGATCCTGAGAAGAAACGTCGAAGAAATCATTCTTTGCTATGACTCTGATAAAGCCGGTTATGAAGCC
ACCTTAAAAGCTTCGGAGCTTCTGCAAAAAAAAGGCTGCAAAGTCAGAGTTGCAATGATTCCTGACGGATTGGACCCTGA
TGATTACATCAAAAAATTCGGCGGGGAAAAATTTAAAAACGACATTATTGACGCAAGTGTCACCGTAATGGCGTTCAAAA
TGCAATATTTCCGAAAAGGAAAGAACCTGTCCGATGAAGGCGACCGCCTAGCTTACATTAAAGACGTACTGAAAGAAATC
AGCACGCTTTCAGGGTCTCTAGAGCAGGAAGTCTATGTAAAGCAGCTTGCTTCAGAGTTTTCGCTTTCACAGGAGTCTTT
AACTGAGCAGCTGTCTGTTTTCAGCAAGCAAAACAAACCTGCTGACAATAGCGGTGAAACTAAAACGCGGCGAGCGCATC
TGACGACAAAAGCAAGGCAAAAACGTTTGCGTCCGGCGTATGAAAATGCAGAAAGGCTGTTACTCGCTCACATGCTTCGA
GATCGGAGCGTCATCAAAAAAGTGATTGACCGGGTAGGGTTTCAATTTAATATTGATGAGCACCGGGCATTAGCCGCTTA
TCTTTATGCTTTTTATGAAGAGGGAGCCGAGCTGACGCCTCAGCATCTGATGGCCAGGGTGACGGATGATCATATAAGCC
AGCTCTTGTCCGATATATTAATGCTTCAGGTTAATCAAGAGCTTAGCGAAGCCGAGTTATCAGATTATGTAAAAAAAGTG
TTGAATCAAAGAAATTGGTCAATGATAAAAGAAAAAGAGGCGGAAAGAGCCGAAGCAGAAAGGCAAAAAGATTTTTTAAG
AGCTGCTTCTTTGGCTCAAGAAATCGTTACATTGAACCGATCTTTAAAATAA

Upstream 100 bases:

>100_bases
AGATCGAGAACGATTTATTACACTGCTGCAAAAAATCCTGTCGAACGATGAAGGGATTTTGCACTAAAGCATCGAATAAT
GTACGACGGAGTGTTATAAG

Downstream 100 bases:

>100_bases
CTGGAGAACTGATGAGGAGCATTTATTGGCAATGATTCCTTGCGGAGGAGCAAATAGATCGCTTAACCTCATCATGAATT
GTCATTTCATTATTCGCACA

Product: DNA primase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 603; Mature: 602

Protein sequence:

>603_residues
MGNRIPDEIVDQVQKSADIVEVIGDYVQLKKQGRNYFGLCPFHGESTPSFSVSPDKQIFHCFGCGAGGNVFSFLRQMEGY
SFAESVSHLADKYQIDFPDDITVHSGARPESSGEQKMAEAHELLKKFYHHLLINTKEGQEALDYLLSRGFTKELINEFQI
GYALDSWDFITKFLVKRGFSEAQMEKAGLLIRREDGSGYFDRFRNRVMFPIHDHHGAVVAFSGRALGSQQPKYMNSPETP
LFHKSKLLYNFYKARLHIRKQERAVLFEGFADVISAVSSDVKESIATMGTSLTDDHVKILRRNVEEIILCYDSDKAGYEA
TLKASELLQKKGCKVRVAMIPDGLDPDDYIKKFGGEKFKNDIIDASVTVMAFKMQYFRKGKNLSDEGDRLAYIKDVLKEI
STLSGSLEQEVYVKQLASEFSLSQESLTEQLSVFSKQNKPADNSGETKTRRAHLTTKARQKRLRPAYENAERLLLAHMLR
DRSVIKKVIDRVGFQFNIDEHRALAAYLYAFYEEGAELTPQHLMARVTDDHISQLLSDILMLQVNQELSEAELSDYVKKV
LNQRNWSMIKEKEAERAEAERQKDFLRAASLAQEIVTLNRSLK

Sequences:

>Translated_603_residues
MGNRIPDEIVDQVQKSADIVEVIGDYVQLKKQGRNYFGLCPFHGESTPSFSVSPDKQIFHCFGCGAGGNVFSFLRQMEGY
SFAESVSHLADKYQIDFPDDITVHSGARPESSGEQKMAEAHELLKKFYHHLLINTKEGQEALDYLLSRGFTKELINEFQI
GYALDSWDFITKFLVKRGFSEAQMEKAGLLIRREDGSGYFDRFRNRVMFPIHDHHGAVVAFSGRALGSQQPKYMNSPETP
LFHKSKLLYNFYKARLHIRKQERAVLFEGFADVISAVSSDVKESIATMGTSLTDDHVKILRRNVEEIILCYDSDKAGYEA
TLKASELLQKKGCKVRVAMIPDGLDPDDYIKKFGGEKFKNDIIDASVTVMAFKMQYFRKGKNLSDEGDRLAYIKDVLKEI
STLSGSLEQEVYVKQLASEFSLSQESLTEQLSVFSKQNKPADNSGETKTRRAHLTTKARQKRLRPAYENAERLLLAHMLR
DRSVIKKVIDRVGFQFNIDEHRALAAYLYAFYEEGAELTPQHLMARVTDDHISQLLSDILMLQVNQELSEAELSDYVKKV
LNQRNWSMIKEKEAERAEAERQKDFLRAASLAQEIVTLNRSLK
>Mature_602_residues
GNRIPDEIVDQVQKSADIVEVIGDYVQLKKQGRNYFGLCPFHGESTPSFSVSPDKQIFHCFGCGAGGNVFSFLRQMEGYS
FAESVSHLADKYQIDFPDDITVHSGARPESSGEQKMAEAHELLKKFYHHLLINTKEGQEALDYLLSRGFTKELINEFQIG
YALDSWDFITKFLVKRGFSEAQMEKAGLLIRREDGSGYFDRFRNRVMFPIHDHHGAVVAFSGRALGSQQPKYMNSPETPL
FHKSKLLYNFYKARLHIRKQERAVLFEGFADVISAVSSDVKESIATMGTSLTDDHVKILRRNVEEIILCYDSDKAGYEAT
LKASELLQKKGCKVRVAMIPDGLDPDDYIKKFGGEKFKNDIIDASVTVMAFKMQYFRKGKNLSDEGDRLAYIKDVLKEIS
TLSGSLEQEVYVKQLASEFSLSQESLTEQLSVFSKQNKPADNSGETKTRRAHLTTKARQKRLRPAYENAERLLLAHMLRD
RSVIKKVIDRVGFQFNIDEHRALAAYLYAFYEEGAELTPQHLMARVTDDHISQLLSDILMLQVNQELSEAELSDYVKKVL
NQRNWSMIKEKEAERAEAERQKDFLRAASLAQEIVTLNRSLK

Specific function: DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments on both template strands at replication forks during chromosomal DNA synthesis

COG id: COG0358

COG function: function code L; DNA primase (bacterial type)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain

Homologues:

Organism=Escherichia coli, GI1789447, Length=438, Percent_Identity=35.3881278538813, Blast_Score=276, Evalue=2e-75,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PRIM_BACSU (P05096)

Other databases:

- EMBL:   M10040
- EMBL:   X03897
- EMBL:   D84432
- EMBL:   AL009126
- PIR:   A22282
- RefSeq:   NP_390400.2
- ProteinModelPortal:   P05096
- DIP:   DIP-46405N
- EnsemblBacteria:   EBBACT00000003078
- GeneID:   937899
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU25210
- NMPDR:   fig|224308.1.peg.2525
- GenoList:   BSU25210
- GeneTree:   EBGT00050000002869
- HOGENOM:   HBG573408
- PhylomeDB:   P05096
- ProtClustDB:   PRK05667
- BioCyc:   BSUB:BSU25210-MONOMER
- InterPro:   IPR007693
- InterPro:   IPR013264
- InterPro:   IPR019475
- InterPro:   IPR006295
- InterPro:   IPR006171
- InterPro:   IPR002694
- Gene3D:   G3DSA:3.90.980.10
- Gene3D:   G3DSA:3.90.580.10
- SMART:   SM00493
- SMART:   SM00400
- TIGRFAMs:   TIGR01391

Pfam domain/function: PF10410 DnaB_bind; PF01751 Toprim; PF08275 Toprim_N; PF01807 zf-CHC2; SSF48024 DNA_helicase_DnaB-like_N

EC number: 2.7.7.-

Molecular weight: Translated: 68737; Mature: 68606

Theoretical pI: Translated: 7.16; Mature: 7.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGNRIPDEIVDQVQKSADIVEVIGDYVQLKKQGRNYFGLCPFHGESTPSFSVSPDKQIFH
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCHHEEE
CFGCGAGGNVFSFLRQMEGYSFAESVSHLADKYQIDFPDDITVHSGARPESSGEQKMAEA
EECCCCCCHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHH
HELLKKFYHHLLINTKEGQEALDYLLSRGFTKELINEFQIGYALDSWDFITKFLVKRGFS
HHHHHHHHHHHHCCCCCHHHHHHHHHHCCCHHHHHHHHHHCCEECHHHHHHHHHHHCCCC
EAQMEKAGLLIRREDGSGYFDRFRNRVMFPIHDHHGAVVAFSGRALGSQQPKYMNSPETP
HHHHHHCCCEEEECCCCCHHHHHCCCEEEEEECCCCEEEEECCCCCCCCCCCCCCCCCCC
LFHKSKLLYNFYKARLHIRKQERAVLFEGFADVISAVSSDVKESIATMGTSLTDDHVKIL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
RRNVEEIILCYDSDKAGYEATLKASELLQKKGCKVRVAMIPDGLDPDDYIKKFGGEKFKN
HHCHHHEEEEECCCCCCCHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHCCHHHHH
DIIDASVTVMAFKMQYFRKGKNLSDEGDRLAYIKDVLKEISTLSGSLEQEVYVKQLASEF
HHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
SLSQESLTEQLSVFSKQNKPADNSGETKTRRAHLTTKARQKRLRPAYENAERLLLAHMLR
CCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
DRSVIKKVIDRVGFQFNIDEHRALAAYLYAFYEEGAELTPQHLMARVTDDHISQLLSDIL
HHHHHHHHHHHCCCEECCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHH
MLQVNQELSEAELSDYVKKVLNQRNWSMIKEKEAERAEAERQKDFLRAASLAQEIVTLNR
HHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SLK
CCC
>Mature Secondary Structure 
GNRIPDEIVDQVQKSADIVEVIGDYVQLKKQGRNYFGLCPFHGESTPSFSVSPDKQIFH
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCHHEEE
CFGCGAGGNVFSFLRQMEGYSFAESVSHLADKYQIDFPDDITVHSGARPESSGEQKMAEA
EECCCCCCHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHH
HELLKKFYHHLLINTKEGQEALDYLLSRGFTKELINEFQIGYALDSWDFITKFLVKRGFS
HHHHHHHHHHHHCCCCCHHHHHHHHHHCCCHHHHHHHHHHCCEECHHHHHHHHHHHCCCC
EAQMEKAGLLIRREDGSGYFDRFRNRVMFPIHDHHGAVVAFSGRALGSQQPKYMNSPETP
HHHHHHCCCEEEECCCCCHHHHHCCCEEEEEECCCCEEEEECCCCCCCCCCCCCCCCCCC
LFHKSKLLYNFYKARLHIRKQERAVLFEGFADVISAVSSDVKESIATMGTSLTDDHVKIL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
RRNVEEIILCYDSDKAGYEATLKASELLQKKGCKVRVAMIPDGLDPDDYIKKFGGEKFKN
HHCHHHEEEEECCCCCCCHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHCCHHHHH
DIIDASVTVMAFKMQYFRKGKNLSDEGDRLAYIKDVLKEISTLSGSLEQEVYVKQLASEF
HHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
SLSQESLTEQLSVFSKQNKPADNSGETKTRRAHLTTKARQKRLRPAYENAERLLLAHMLR
CCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
DRSVIKKVIDRVGFQFNIDEHRALAAYLYAFYEEGAELTPQHLMARVTDDHISQLLSDIL
HHHHHHHHHHHCCCEECCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHH
MLQVNQELSEAELSDYVKKVLNQRNWSMIKEKEAERAEAERQKDFLRAASLAQEIVTLNR
HHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SLK
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups; Nucleotidyltransferases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3919021; 3086839; 8969508; 9384377