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The map label for this gene is sigA

Identifier: 255767573

GI number: 255767573

Start: 2600214

End: 2601329

Strand: Reverse

Name: sigA

Synonym: BSU25200

Alternate gene names: 255767573

Gene position: 2601329-2600214 (Counterclockwise)

Preceding gene: 255767574

Following gene: 16079574

Centisome position: 61.71

GC content: 42.47

Gene sequence:

>1116_bases
ATGGCTGATAAACAAACCCACGAGACAGAATTAACATTCGACCAAGTAAAAGAGCAATTAACAGAGTCTGGTAAAAAACG
TGGCGTTTTGACATATGAAGAAATTGCTGAGCGTATGTCCAGCTTTGAAATTGAATCAGACCAAATGGATGAGTATTATG
AATTTTTAGGTGAACAAGGTGTTGAATTAATTAGTGAGAATGAAGAAACAGAAGATCCTAATATTCAGCAGCTTGCCAAA
GCCGAAGAAGAATTTGACCTTAATGACCTAAGTGTACCGCCTGGCGTTAAAATCAATGACCCAGTTCGTATGTATTTAAA
GGAAATCGGTCGGGTTAACCTTCTTTCTGCAAAAGAAGAAATCGCCTACGCTCAAAAGATTGAAGAAGGTGACGAAGAAT
CTAAACGCAGATTGGCTGAAGCGAACCTGCGGCTTGTTGTCAGTATCGCAAAACGGTATGTCGGACGCGGTATGCTGTTC
CTTGATCTGATCCAGGAAGGAAACATGGGCCTGATGAAAGCCGTTGAAAAATTTGATTATCGCAAAGGTTATAAATTCAG
TACGTATGCTACGTGGTGGATCAGACAGGCGATTACACGCGCCATTGCCGATCAGGCGAGAACGATCCGGATTCCCGTTC
ATATGGTTGAAACCATTAATAAATTAATCCGTGTGCAGCGTCAATTACTGCAAGACTTAGGCAGAGAACCAACACCTGAA
GAAATTGCGGAAGATATGGATTTAACGCCTGAAAAAGTACGCGAAATCTTAAAGATTGCTCAAGAGCCGGTATCTCTGGA
AACACCGATCGGTGAAGAGGATGACTCGCACCTTGGTGATTTCATTGAAGACCAAGAAGCAACTTCACCTTCTGACCACG
CCGCATACGAGCTATTGAAAGAGCAGCTGGAAGATGTGCTTGATACGTTAACTGATCGTGAAGAAAATGTATTGCGTCTT
CGATTCGGTCTTGATGACGGCCGTACAAGAACATTAGAAGAGGTCGGCAAAGTATTTGGAGTAACGAGAGAGCGTATTCG
ACAAATCGAAGCCAAAGCGTTGCGGAAACTAAGACATCCTAGCAGAAGTAAACGTTTGAAAGATTTCCTTGAATAA

Upstream 100 bases:

>100_bases
CATTGTTAAAGGCAGTTCACATAGAAAACGCCTGAATGGACCGAATAAGAATCATACCGCTTATAGAATTCGTTGCAAGC
TTTGGAAGGAGGGATCCATA

Downstream 100 bases:

>100_bases
GATGGAACGGGTCTTGAAGATCCGTTCTTCTTTTTTTAAAAAGATATATGGATAATATGCCTTTATTTTACTGAAAAATG
ATGTCATTTGCAAATGAACA

Product: RNA polymerase sigma factor RpoD

Products: NA

Alternate protein names: Sigma-43; Sigma-A

Number of amino acids: Translated: 371; Mature: 370

Protein sequence:

>371_residues
MADKQTHETELTFDQVKEQLTESGKKRGVLTYEEIAERMSSFEIESDQMDEYYEFLGEQGVELISENEETEDPNIQQLAK
AEEEFDLNDLSVPPGVKINDPVRMYLKEIGRVNLLSAKEEIAYAQKIEEGDEESKRRLAEANLRLVVSIAKRYVGRGMLF
LDLIQEGNMGLMKAVEKFDYRKGYKFSTYATWWIRQAITRAIADQARTIRIPVHMVETINKLIRVQRQLLQDLGREPTPE
EIAEDMDLTPEKVREILKIAQEPVSLETPIGEEDDSHLGDFIEDQEATSPSDHAAYELLKEQLEDVLDTLTDREENVLRL
RFGLDDGRTRTLEEVGKVFGVTRERIRQIEAKALRKLRHPSRSKRLKDFLE

Sequences:

>Translated_371_residues
MADKQTHETELTFDQVKEQLTESGKKRGVLTYEEIAERMSSFEIESDQMDEYYEFLGEQGVELISENEETEDPNIQQLAK
AEEEFDLNDLSVPPGVKINDPVRMYLKEIGRVNLLSAKEEIAYAQKIEEGDEESKRRLAEANLRLVVSIAKRYVGRGMLF
LDLIQEGNMGLMKAVEKFDYRKGYKFSTYATWWIRQAITRAIADQARTIRIPVHMVETINKLIRVQRQLLQDLGREPTPE
EIAEDMDLTPEKVREILKIAQEPVSLETPIGEEDDSHLGDFIEDQEATSPSDHAAYELLKEQLEDVLDTLTDREENVLRL
RFGLDDGRTRTLEEVGKVFGVTRERIRQIEAKALRKLRHPSRSKRLKDFLE
>Mature_370_residues
ADKQTHETELTFDQVKEQLTESGKKRGVLTYEEIAERMSSFEIESDQMDEYYEFLGEQGVELISENEETEDPNIQQLAKA
EEEFDLNDLSVPPGVKINDPVRMYLKEIGRVNLLSAKEEIAYAQKIEEGDEESKRRLAEANLRLVVSIAKRYVGRGMLFL
DLIQEGNMGLMKAVEKFDYRKGYKFSTYATWWIRQAITRAIADQARTIRIPVHMVETINKLIRVQRQLLQDLGREPTPEE
IAEDMDLTPEKVREILKIAQEPVSLETPIGEEDDSHLGDFIEDQEATSPSDHAAYELLKEQLEDVLDTLTDREENVLRLR
FGLDDGRTRTLEEVGKVFGVTRERIRQIEAKALRKLRHPSRSKRLKDFLE

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This is the primary sigma factor of this bacterium

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family

Homologues:

Organism=Escherichia coli, GI1789448, Length=238, Percent_Identity=69.327731092437, Blast_Score=347, Evalue=1e-96,
Organism=Escherichia coli, GI1789098, Length=321, Percent_Identity=42.9906542056075, Blast_Score=243, Evalue=1e-65,
Organism=Escherichia coli, GI1789871, Length=272, Percent_Identity=31.25, Blast_Score=114, Evalue=1e-26,
Organism=Escherichia coli, GI1788231, Length=202, Percent_Identity=29.7029702970297, Blast_Score=69, Evalue=6e-13,

Paralogues:

None

Copy number: 700 (log & stationary phase) [C]

Swissprot (AC and ID): RPOD_BACSU (P06224)

Other databases:

- EMBL:   M10089
- EMBL:   X03897
- EMBL:   D84432
- EMBL:   AL009126
- EMBL:   J05569
- PIR:   A22626
- RefSeq:   NP_390399.2
- ProteinModelPortal:   P06224
- SMR:   P06224
- EnsemblBacteria:   EBBACT00000003565
- GeneID:   937897
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU25200
- NMPDR:   fig|224308.1.peg.2524
- GenoList:   BSU25200
- GeneTree:   EBGT00070000031774
- HOGENOM:   HBG745096
- PhylomeDB:   P06224
- ProtClustDB:   PRK09210
- BioCyc:   BSUB:BSU25200-MONOMER
- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR007127
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012760
- InterPro:   IPR011991
- Gene3D:   G3DSA:1.10.10.10
- PRINTS:   PR00046
- TIGRFAMs:   TIGR02393
- TIGRFAMs:   TIGR02937

Pfam domain/function: PF03979 Sigma70_r1_1; PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4; SSF88946 Sigma_r2; SSF88659 Sigma_r3_r4

EC number: NA

Molecular weight: Translated: 42949; Mature: 42818

Theoretical pI: Translated: 4.51; Mature: 4.51

Prosite motif: PS00715 SIGMA70_1; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADKQTHETELTFDQVKEQLTESGKKRGVLTYEEIAERMSSFEIESDQMDEYYEFLGEQG
CCCCCCCHHHCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
VELISENEETEDPNIQQLAKAEEEFDLNDLSVPPGVKINDPVRMYLKEIGRVNLLSAKEE
HHHHHCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHH
IAYAQKIEEGDEESKRRLAEANLRLVVSIAKRYVGRGMLFLDLIQEGNMGLMKAVEKFDY
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHH
RKGYKFSTYATWWIRQAITRAIADQARTIRIPVHMVETINKLIRVQRQLLQDLGREPTPE
HCCCCCHHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCHH
EIAEDMDLTPEKVREILKIAQEPVSLETPIGEEDDSHLGDFIEDQEATSPSDHAAYELLK
HHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHH
EQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVGKVFGVTRERIRQIEAKALRKLRHP
HHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
SRSKRLKDFLE
HHHHHHHHHHC
>Mature Secondary Structure 
ADKQTHETELTFDQVKEQLTESGKKRGVLTYEEIAERMSSFEIESDQMDEYYEFLGEQG
CCCCCCHHHCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
VELISENEETEDPNIQQLAKAEEEFDLNDLSVPPGVKINDPVRMYLKEIGRVNLLSAKEE
HHHHHCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHH
IAYAQKIEEGDEESKRRLAEANLRLVVSIAKRYVGRGMLFLDLIQEGNMGLMKAVEKFDY
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHH
RKGYKFSTYATWWIRQAITRAIADQARTIRIPVHMVETINKLIRVQRQLLQDLGREPTPE
HCCCCCHHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCHH
EIAEDMDLTPEKVREILKIAQEPVSLETPIGEEDDSHLGDFIEDQEATSPSDHAAYELLK
HHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHH
EQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVGKVFGVTRERIRQIEAKALRKLRHP
HHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
SRSKRLKDFLE
HHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2987246; 3086839; 8969508; 9384377; 2166045; 8363585