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The map label for this gene is ymfH

Identifier: 255767384

GI number: 255767384

Start: 1758314

End: 1759600

Strand: Direct

Name: ymfH

Synonym: BSU16860

Alternate gene names: 255767384

Gene position: 1758314-1759600 (Clockwise)

Preceding gene: 255767383

Following gene: 255767385

Centisome position: 41.71

GC content: 42.27

Gene sequence:

>1287_bases
TTGATCAAACCAATCGAATATGAACAGCTTCAGGAGACGCTGTATCATGAAAAAATGTCCAACGGCCTTGATGTTTACGT
TTTGCCGAAAAAAGGCTTCAACAAGACATATGCGGTCTTTACAACAAAGTACGGCTCGATAGATAACCGGTTTGTCCCTT
TAGGTAAAAACGAGATGGTTCACGTGCCGGACGGGATTGCTCACTTTCTTGAGCACAAGCTGTTTGAGAAAGCGGACGGA
GACGTTTTTCAAGATTTCAGCAAACAGGGGGCTTCTGCCAATGCGTTTACGTCATTTACAAGAACGGCTTACCTTTTCTC
AAGCACATCAAATGTTGAACGCAATTTAGAGACGCTTATCGATTTCGTACAGGACCCATATTTTACTGAAAAAACGGTTG
AAAAGGAAAAAGGGATTATCGGGCAGGAGATTAATATGTACGACGATAACCCTGATTGGAGGCTTTACTTCGGGGTCATT
GAAAACATGTACAAAGAGCATCCTGTCAGAATTGACATAGCGGGAACAGTGGAAAGCATTTCACATATTACAAAAGACCT
TCTTTATGAATGCTATGAAACGTTTTATCACCCGAGTAACATGCTCCTTTTCATTGTCGGCCCTGTAGATCCTGAAGCGA
TTATTTCTCAGGTAAGAGAAAACCAGGGGAAAAAGCCGTATACTGATCAGCCGGAGATCAAACGAGAAGAAGTGAAAGAG
CAAGAAGCGGTTTTCCGAAAAGAAAAAGAGATCAAAATGAACGTGCAGGGACCGAAATGCCTTGTTGGGCTGAAATCCAA
AAATCCGTTTAAATTAGGCAAAGAGCTCTTAAAGCATGAACTTTCAATGAACTTATTGCTTGAAGCTCTTTTTGGCAAAA
GCTCTGCCCAGTATGAATCACTTTATGAAAAAGGATATATTGACGAAACGTTCAGCTTTGATTTTACTGCTGAATATGGG
TTCGGTTTTGCGGCGATCGGCGGAGATACGCCTGAGCCTGATCAATTGGCTGAAGACATTTCAAGCATGCTTTTGCGCGC
CGGTGAACTGATTACTGCTGAAAAGATTGAACTTGCCAGAAAGAAAAAGATCGGCACATTCTTAAAAGCGCTGAATTCCC
CTGAATACATCGCCAATCAATTTACCCGTTATGCGTTCTTGGATATGAGCCTGTTTGATGTTGTAACGGTACTCGAGCAG
ATTACCCTCGAGGATGTCCAGAACGTAATACAAGAGGAAATCGCTGCAGACAGACTGACTGTCTGCAAGGTTGTTCCTAA
ATCATAA

Upstream 100 bases:

>100_bases
TTGCCAATATTGAGAATGTCACAAAAGAGGATATCATTAAAGCCGGCGAAAAGATTCAGCTTGATACGACTTATTTCTTA
AAAGGGACGGAGGGTGCATC

Downstream 100 bases:

>100_bases
ACAAAACATCCCTCCAGTGTGAGGGGTGTTTTTCTGCGGAAAGAAGGAAAGAGGATGAACAAAACAGCACTAATCACCGG
AGCAAGCGGCGGCATTGGCA

Product: processing protease

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 428; Mature: 428

Protein sequence:

>428_residues
MIKPIEYEQLQETLYHEKMSNGLDVYVLPKKGFNKTYAVFTTKYGSIDNRFVPLGKNEMVHVPDGIAHFLEHKLFEKADG
DVFQDFSKQGASANAFTSFTRTAYLFSSTSNVERNLETLIDFVQDPYFTEKTVEKEKGIIGQEINMYDDNPDWRLYFGVI
ENMYKEHPVRIDIAGTVESISHITKDLLYECYETFYHPSNMLLFIVGPVDPEAIISQVRENQGKKPYTDQPEIKREEVKE
QEAVFRKEKEIKMNVQGPKCLVGLKSKNPFKLGKELLKHELSMNLLLEALFGKSSAQYESLYEKGYIDETFSFDFTAEYG
FGFAAIGGDTPEPDQLAEDISSMLLRAGELITAEKIELARKKKIGTFLKALNSPEYIANQFTRYAFLDMSLFDVVTVLEQ
ITLEDVQNVIQEEIAADRLTVCKVVPKS

Sequences:

>Translated_428_residues
MIKPIEYEQLQETLYHEKMSNGLDVYVLPKKGFNKTYAVFTTKYGSIDNRFVPLGKNEMVHVPDGIAHFLEHKLFEKADG
DVFQDFSKQGASANAFTSFTRTAYLFSSTSNVERNLETLIDFVQDPYFTEKTVEKEKGIIGQEINMYDDNPDWRLYFGVI
ENMYKEHPVRIDIAGTVESISHITKDLLYECYETFYHPSNMLLFIVGPVDPEAIISQVRENQGKKPYTDQPEIKREEVKE
QEAVFRKEKEIKMNVQGPKCLVGLKSKNPFKLGKELLKHELSMNLLLEALFGKSSAQYESLYEKGYIDETFSFDFTAEYG
FGFAAIGGDTPEPDQLAEDISSMLLRAGELITAEKIELARKKKIGTFLKALNSPEYIANQFTRYAFLDMSLFDVVTVLEQ
ITLEDVQNVIQEEIAADRLTVCKVVPKS
>Mature_428_residues
MIKPIEYEQLQETLYHEKMSNGLDVYVLPKKGFNKTYAVFTTKYGSIDNRFVPLGKNEMVHVPDGIAHFLEHKLFEKADG
DVFQDFSKQGASANAFTSFTRTAYLFSSTSNVERNLETLIDFVQDPYFTEKTVEKEKGIIGQEINMYDDNPDWRLYFGVI
ENMYKEHPVRIDIAGTVESISHITKDLLYECYETFYHPSNMLLFIVGPVDPEAIISQVRENQGKKPYTDQPEIKREEVKE
QEAVFRKEKEIKMNVQGPKCLVGLKSKNPFKLGKELLKHELSMNLLLEALFGKSSAQYESLYEKGYIDETFSFDFTAEYG
FGFAAIGGDTPEPDQLAEDISSMLLRAGELITAEKIELARKKKIGTFLKALNSPEYIANQFTRYAFLDMSLFDVVTVLEQ
ITLEDVQNVIQEEIAADRLTVCKVVPKS

Specific function: Unknown

COG id: COG0612

COG function: function code R; Predicted Zn-dependent peptidases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M16 family

Homologues:

Organism=Escherichia coli, GI1787770, Length=187, Percent_Identity=24.5989304812834, Blast_Score=68, Evalue=9e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): YMFH_BACSU (O31766)

Other databases:

- EMBL:   AL009126
- RefSeq:   NP_389568.2
- ProteinModelPortal:   O31766
- MEROPS:   M16.014
- EnsemblBacteria:   EBBACT00000000304
- GeneID:   939665
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU16860
- GenoList:   BSU16860
- GeneTree:   EBGT00050000001968
- HOGENOM:   HBG304809
- ProtClustDB:   CLSK2765278
- BioCyc:   BSUB:BSU16860-MONOMER
- GO:   GO:0006508
- InterPro:   IPR011249
- InterPro:   IPR011237
- InterPro:   IPR011765
- InterPro:   IPR007863
- Gene3D:   G3DSA:3.30.830.10

Pfam domain/function: PF00675 Peptidase_M16; PF05193 Peptidase_M16_C; SSF63411 Metalloenz_metal-bd

EC number: 3.4.99.- [C]

Molecular weight: Translated: 48949; Mature: 48949

Theoretical pI: Translated: 4.75; Mature: 4.75

Prosite motif: PS00143 INSULINASE

Important sites: ACT_SITE 71-71

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKPIEYEQLQETLYHEKMSNGLDVYVLPKKGFNKTYAVFTTKYGSIDNRFVPLGKNEMV
CCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCEEEEEEEECCCCCCCEEECCCCCEE
HVPDGIAHFLEHKLFEKADGDVFQDFSKQGASANAFTSFTRTAYLFSSTSNVERNLETLI
ECCHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCHHHHHHHHHHHEEECCCHHHHHHHHHH
DFVQDPYFTEKTVEKEKGIIGQEINMYDDNPDWRLYFGVIENMYKEHPVRIDIAGTVESI
HHHHCCCCCHHHHHHHCCCCCCCCCCCCCCCCCEEHHHHHHHHHHHCCEEEEECCHHHHH
SHITKDLLYECYETFYHPSNMLLFIVGPVDPEAIISQVRENQGKKPYTDQPEIKREEVKE
HHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHH
QEAVFRKEKEIKMNVQGPKCLVGLKSKNPFKLGKELLKHELSMNLLLEALFGKSSAQYES
HHHHHHHHHCEEEECCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
LYEKGYIDETFSFDFTAEYGFGFAAIGGDTPEPDQLAEDISSMLLRAGELITAEKIELAR
HHHCCCCCCCCCCEEEECCCCEEEECCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHH
KKKIGTFLKALNSPEYIANQFTRYAFLDMSLFDVVTVLEQITLEDVQNVIQEEIAADRLT
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VCKVVPKS
HHEECCCC
>Mature Secondary Structure
MIKPIEYEQLQETLYHEKMSNGLDVYVLPKKGFNKTYAVFTTKYGSIDNRFVPLGKNEMV
CCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCEEEEEEEECCCCCCCEEECCCCCEE
HVPDGIAHFLEHKLFEKADGDVFQDFSKQGASANAFTSFTRTAYLFSSTSNVERNLETLI
ECCHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCHHHHHHHHHHHEEECCCHHHHHHHHHH
DFVQDPYFTEKTVEKEKGIIGQEINMYDDNPDWRLYFGVIENMYKEHPVRIDIAGTVESI
HHHHCCCCCHHHHHHHCCCCCCCCCCCCCCCCCEEHHHHHHHHHHHCCEEEEECCHHHHH
SHITKDLLYECYETFYHPSNMLLFIVGPVDPEAIISQVRENQGKKPYTDQPEIKREEVKE
HHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHH
QEAVFRKEKEIKMNVQGPKCLVGLKSKNPFKLGKELLKHELSMNLLLEALFGKSSAQYES
HHHHHHHHHCEEEECCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
LYEKGYIDETFSFDFTAEYGFGFAAIGGDTPEPDQLAEDISSMLLRAGELITAEKIELAR
HHHCCCCCCCCCCEEEECCCCEEEECCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHH
KKKIGTFLKALNSPEYIANQFTRYAFLDMSLFDVVTVLEQITLEDVQNVIQEEIAADRLT
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VCKVVPKS
HHEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Zn [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on peptide bonds (Peptidases); Endopeptidases of unknown catalytic mechanism [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377