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The map label for this gene is rnjB

Identifier: 255767378

GI number: 255767378

Start: 1749418

End: 1751085

Strand: Direct

Name: rnjB

Synonym: BSU16780

Alternate gene names: 255767378

Gene position: 1749418-1751085 (Clockwise)

Preceding gene: 16078740

Following gene: 255767379

Centisome position: 41.5

GC content: 42.75

Gene sequence:

>1668_bases
TTGAAAAAGAAAAATACAGAAAACGTTAGAATTATCGCCCTTGGCGGTGTCGGAGAGATTGGGAAGAACCTTTATGTCAT
TGAAATTGACTCAGACATATTTGTCGTTGATGCCGGCCTTATGCATCCAGAAAACGAAATGCTCGGTATTGATGTAGTGA
TTCCTGACATATCATATTTGATTGAACGGGCTGACCGCGTCAAGGCAATCTTTTTAACGCACGGGCACGATGAAAATATC
GGCGGCGTTTTTTACTTATTAAACAAGCTGTCCGTTCCAGTGTACGGAACAAAACTGACGCTTGCACTGTTAAGAGAGAA
ATTAAAACAATACGGACATAACCGAAAAACTGATTTAAGAGAAATTCACTCCAAATCTGTCATTACATTTGAATCAACAA
AGGTATCGTTCTTTAGAACGATTCACAGCATTCCTGATTCTGTAGGTGTGAGCTTTAAAACATCACTTGGATCTATTGTA
TGCACGGGAGACTTCAAATTTGACCAAACACCTGCACTTAATCAGACGTGCGACATTGGCGAGATCGCCAAGATCGGCAA
TAGCGGCGTGCTCGCTCTGCTTTCTGACAGTGCAAATGCGGAACGGCCGGGTTACACCCCTTCGGAAGCGGCTGTCAGCG
GAGAAATTTCGGATGCGCTGTATAATTCACAGAACCGGGTGATTATTGCTGTTTTCGCATCCAATATTAACCGGATTCAG
CAAGTCATTCATGCAGCGGCTCAAAATGGCAGAAAGATTGCCGTAGCCGGAAAAAATCTTCAATCTGTCCTGCAGCTGGC
GAGAAAGTTAGGATATATTGAAGCAGATGATGAATTGTTTATTTCTGTACAGGATGTCAAAAAATATCCGAAGCGCGAAG
TGGCCATTATTACAGCCGGAAGCCAAGGTGAACCATTAGCTGCTTTGACAAGAATGGCAAACAAAGCTCACAAACAGCTG
AACATTGAAGAAGGGGATACGGTGGTCATTGCGTCAACTCCGATTCCCGGGCAGGAACTCATTTACTCTAAAACAGTAGA
TCTTCTTGCGAGAGCCGGTGCACAAGTCATCTTTGCCCAAAAGCGTGTTCATGTATCAGGCCACGGCTCACAAGAAGAGT
TAAAGCTGATGATCAACCTGCTTAAACCAAAATATTTGATTCCTGTAAACGGCGAATACAGAATGCAAAAAGCTCATTCA
AAAATCGCTGAAGAAACCGGCATGAAGCGCAGTGACATCTTCTTAATTGAAAAAGGCGACGTCGTTGAATTCCGCGGCCA
AAACGTAAAAATTGGGGATAAAGTTCCTTACGGCAATATTTTAATTGATGGTTTAGGTGTCGGTGATATCGGCAATATCG
TATTGAGAGACCGCCGCTTGCTTTCACAGGACGGAATTTTAATCGTTGTCATTACACTTGATAAACAAAAGAAACATCTT
GTATCAGGACCAGAAATTATCACTCGCGGCTTTGTTTATGTAAGAGAGTCTGAAGGTCTTATCGTACAGGCTACTGAGCT
TGTACGGTCCATCGTGACAGAAGCGACTGAAACATCAAATGTCGAATGGTCAACGCTGAAGCAGGCAATGCGGGATGCTT
TGAACCAATTCCTTTATGAAAAAACGAAACGTAAACCGATGATCATCCCAATTATTATGGAAGTATAA

Upstream 100 bases:

>100_bases
TTCCCTAAACCTTCTGCTTTATCTTGTTTTCTAAATTTACTTTCAGTTATAATAGGAACAAGTGATGATGGACGGGTATG
AGACTAGGAGGATATAGATT

Downstream 100 bases:

>100_bases
TGACTGACTAAAGACCGGAGCTGCTCCGGTCTTTATTTTTTGTCCTTCAAGCCGGGATTGAATGAAGTATCTTTGTTCAT
AATACAAAGAGAAACTCGGA

Product: ribonuclease J2

Products: NA

Alternate protein names: RNase J2

Number of amino acids: Translated: 555; Mature: 555

Protein sequence:

>555_residues
MKKKNTENVRIIALGGVGEIGKNLYVIEIDSDIFVVDAGLMHPENEMLGIDVVIPDISYLIERADRVKAIFLTHGHDENI
GGVFYLLNKLSVPVYGTKLTLALLREKLKQYGHNRKTDLREIHSKSVITFESTKVSFFRTIHSIPDSVGVSFKTSLGSIV
CTGDFKFDQTPALNQTCDIGEIAKIGNSGVLALLSDSANAERPGYTPSEAAVSGEISDALYNSQNRVIIAVFASNINRIQ
QVIHAAAQNGRKIAVAGKNLQSVLQLARKLGYIEADDELFISVQDVKKYPKREVAIITAGSQGEPLAALTRMANKAHKQL
NIEEGDTVVIASTPIPGQELIYSKTVDLLARAGAQVIFAQKRVHVSGHGSQEELKLMINLLKPKYLIPVNGEYRMQKAHS
KIAEETGMKRSDIFLIEKGDVVEFRGQNVKIGDKVPYGNILIDGLGVGDIGNIVLRDRRLLSQDGILIVVITLDKQKKHL
VSGPEIITRGFVYVRESEGLIVQATELVRSIVTEATETSNVEWSTLKQAMRDALNQFLYEKTKRKPMIIPIIMEV

Sequences:

>Translated_555_residues
MKKKNTENVRIIALGGVGEIGKNLYVIEIDSDIFVVDAGLMHPENEMLGIDVVIPDISYLIERADRVKAIFLTHGHDENI
GGVFYLLNKLSVPVYGTKLTLALLREKLKQYGHNRKTDLREIHSKSVITFESTKVSFFRTIHSIPDSVGVSFKTSLGSIV
CTGDFKFDQTPALNQTCDIGEIAKIGNSGVLALLSDSANAERPGYTPSEAAVSGEISDALYNSQNRVIIAVFASNINRIQ
QVIHAAAQNGRKIAVAGKNLQSVLQLARKLGYIEADDELFISVQDVKKYPKREVAIITAGSQGEPLAALTRMANKAHKQL
NIEEGDTVVIASTPIPGQELIYSKTVDLLARAGAQVIFAQKRVHVSGHGSQEELKLMINLLKPKYLIPVNGEYRMQKAHS
KIAEETGMKRSDIFLIEKGDVVEFRGQNVKIGDKVPYGNILIDGLGVGDIGNIVLRDRRLLSQDGILIVVITLDKQKKHL
VSGPEIITRGFVYVRESEGLIVQATELVRSIVTEATETSNVEWSTLKQAMRDALNQFLYEKTKRKPMIIPIIMEV
>Mature_555_residues
MKKKNTENVRIIALGGVGEIGKNLYVIEIDSDIFVVDAGLMHPENEMLGIDVVIPDISYLIERADRVKAIFLTHGHDENI
GGVFYLLNKLSVPVYGTKLTLALLREKLKQYGHNRKTDLREIHSKSVITFESTKVSFFRTIHSIPDSVGVSFKTSLGSIV
CTGDFKFDQTPALNQTCDIGEIAKIGNSGVLALLSDSANAERPGYTPSEAAVSGEISDALYNSQNRVIIAVFASNINRIQ
QVIHAAAQNGRKIAVAGKNLQSVLQLARKLGYIEADDELFISVQDVKKYPKREVAIITAGSQGEPLAALTRMANKAHKQL
NIEEGDTVVIASTPIPGQELIYSKTVDLLARAGAQVIFAQKRVHVSGHGSQEELKLMINLLKPKYLIPVNGEYRMQKAHS
KIAEETGMKRSDIFLIEKGDVVEFRGQNVKIGDKVPYGNILIDGLGVGDIGNIVLRDRRLLSQDGILIVVITLDKQKKHL
VSGPEIITRGFVYVRESEGLIVQATELVRSIVTEATETSNVEWSTLKQAMRDALNQFLYEKTKRKPMIIPIIMEV

Specific function: Cleaves the 5'-leader sequence of certain mRNAs. May play a role in maturation and stability of specific mRNAs. Appears to have a limited effect on 16S rRNA maturation, despite its similarity to rnjA

COG id: COG0595

COG function: function code R; Predicted hydrolase of the metallo-beta-lactamase superfamily

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the metallo-beta-lactamase superfamily. RNA-metabolizing metallo-beta-lactamase-like family. RNase J subfamily

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RNJ2_BACSU (O31760)

Other databases:

- EMBL:   AL009126
- PIR:   H69884
- RefSeq:   NP_389560.2
- ProteinModelPortal:   O31760
- SMR:   O31760
- EnsemblBacteria:   EBBACT00000001147
- GeneID:   938463
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU16780
- NMPDR:   fig|224308.1.peg.1681
- GenoList:   BSU16780
- GeneTree:   EBGT00050000000394
- HOGENOM:   HBG497378
- ProtClustDB:   CLSK887316
- BioCyc:   BSUB:BSU16780-MONOMER
- GO:   GO:0005737
- InterPro:   IPR001279
- InterPro:   IPR011108
- InterPro:   IPR004613
- SMART:   SM00849
- TIGRFAMs:   TIGR00649

Pfam domain/function: PF00753 Lactamase_B; PF07521 RMMBL

EC number: NA

Molecular weight: Translated: 61176; Mature: 61176

Theoretical pI: Translated: 8.80; Mature: 8.80

Prosite motif: PS01292 UPF0036

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKKNTENVRIIALGGVGEIGKNLYVIEIDSDIFVVDAGLMHPENEMLGIDVVIPDISYL
CCCCCCCCEEEEEECCCHHHCCCEEEEEECCCEEEEECCCCCCCCCEEEEEEEECCHHHH
IERADRVKAIFLTHGHDENIGGVFYLLNKLSVPVYGTKLTLALLREKLKQYGHNRKTDLR
HHHCCCEEEEEEECCCCCCCCHHHHHHHHCCCCEECHHHHHHHHHHHHHHHCCCCCCHHH
EIHSKSVITFESTKVSFFRTIHSIPDSVGVSFKTSLGSIVCTGDFKFDQTPALNQTCDIG
HHHCCCEEEEECCHHHHHHHHHHCCHHCCCEEEECCCCEEEECCCCCCCCCCCCCCCCCH
EIAKIGNSGVLALLSDSANAERPGYTPSEAAVSGEISDALYNSQNRVIIAVFASNINRIQ
HHHHCCCCCEEEEEECCCCCCCCCCCCCCHHCCCCHHHHHCCCCCCEEEEEECCCHHHHH
QVIHAAAQNGRKIAVAGKNLQSVLQLARKLGYIEADDELFISVQDVKKYPKREVAIITAG
HHHHHHHHCCCEEEEECCCHHHHHHHHHHCCCEECCCCEEEEEHHHHHCCCCEEEEEEEC
SQGEPLAALTRMANKAHKQLNIEEGDTVVIASTPIPGQELIYSKTVDLLARAGAQVIFAQ
CCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCEEEEEE
KRVHVSGHGSQEELKLMINLLKPKYLIPVNGEYRMQKAHSKIAEETGMKRSDIFLIEKGD
EEEEECCCCCHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHHHCCCCCCEEEEECCC
VVEFRGQNVKIGDKVPYGNILIDGLGVGDIGNIVLRDRRLLSQDGILIVVITLDKQKKHL
EEEECCCEEEECCCCCCCCEEEECCCCCCCCHHHHHCCHHHCCCCEEEEEEEECCCCHHH
VSGPEIITRGFVYVRESEGLIVQATELVRSIVTEATETSNVEWSTLKQAMRDALNQFLYE
CCCCCEEECCEEEEECCCCEEEEHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHH
KTKRKPMIIPIIMEV
HHCCCCEEEEEEEEC
>Mature Secondary Structure
MKKKNTENVRIIALGGVGEIGKNLYVIEIDSDIFVVDAGLMHPENEMLGIDVVIPDISYL
CCCCCCCCEEEEEECCCHHHCCCEEEEEECCCEEEEECCCCCCCCCEEEEEEEECCHHHH
IERADRVKAIFLTHGHDENIGGVFYLLNKLSVPVYGTKLTLALLREKLKQYGHNRKTDLR
HHHCCCEEEEEEECCCCCCCCHHHHHHHHCCCCEECHHHHHHHHHHHHHHHCCCCCCHHH
EIHSKSVITFESTKVSFFRTIHSIPDSVGVSFKTSLGSIVCTGDFKFDQTPALNQTCDIG
HHHCCCEEEEECCHHHHHHHHHHCCHHCCCEEEECCCCEEEECCCCCCCCCCCCCCCCCH
EIAKIGNSGVLALLSDSANAERPGYTPSEAAVSGEISDALYNSQNRVIIAVFASNINRIQ
HHHHCCCCCEEEEEECCCCCCCCCCCCCCHHCCCCHHHHHCCCCCCEEEEEECCCHHHHH
QVIHAAAQNGRKIAVAGKNLQSVLQLARKLGYIEADDELFISVQDVKKYPKREVAIITAG
HHHHHHHHCCCEEEEECCCHHHHHHHHHHCCCEECCCCEEEEEHHHHHCCCCEEEEEEEC
SQGEPLAALTRMANKAHKQLNIEEGDTVVIASTPIPGQELIYSKTVDLLARAGAQVIFAQ
CCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCEEEEEE
KRVHVSGHGSQEELKLMINLLKPKYLIPVNGEYRMQKAHSKIAEETGMKRSDIFLIEKGD
EEEEECCCCCHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHHHCCCCCCEEEEECCC
VVEFRGQNVKIGDKVPYGNILIDGLGVGDIGNIVLRDRRLLSQDGILIVVITLDKQKKHL
EEEECCCEEEECCCCCCCCEEEECCCCCCCCHHHHHCCHHHCCCCEEEEEEEECCCCHHH
VSGPEIITRGFVYVRESEGLIVQATELVRSIVTEATETSNVEWSTLKQAMRDALNQFLYE
CCCCCEEECCEEEEECCCCEEEEHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHH
KTKRKPMIIPIIMEV
HHCCCCEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377