Click here to switch to the map view.

The map label for this gene is purU

Identifier: 255767307

GI number: 255767307

Start: 1377243

End: 1378145

Strand: Direct

Name: purU

Synonym: BSU13110

Alternate gene names: 255767307

Gene position: 1377243-1378145 (Clockwise)

Preceding gene: 16078375

Following gene: 255767308

Centisome position: 32.67

GC content: 48.06

Gene sequence:

>903_bases
ATGAAATCATATATGACTCAGCGGTTGGACGAATACCGTGACGGAAATGAGGATAAAGGTCGGCTCTTGGTCAGCTGCCC
CGATCAGCCGGGTATCGTCTCTGCAGTTTCCGCGTTTTTATTTGAACACGGTGCCAATATTATAGAATCAAATCAATATA
CGACAGACCCTGAAGGCGGCCGGTTCTTCCTGAGAATCGAATTCGACTGCGCAGGCATTCGTGAAAAAAAATCATCACTT
CAGGCAGCGTTTGCCTCTGTTGCGGAAAAATTCGACATGACATGGAGCTTAACTTTGGCGAGCGAACTGAAGCGTGTCGC
CATTTTCGTTTCAAAGGAGCTCCACTGCCTGCATGAGCTGATTTGGGAATGGCAAACCGGCAACCTGATGGCGGAGATCG
CTGTTGTCATCAGTAACCATGAGGAAGCGAGAGAGCTGGTTGAGCGCCTGAACATTCCATTCCACTATATGAAAGCGAAC
AAAGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTGGAGCAGTACGATGTTGATGTGATCGTGCTCGCACG
CTATATGCAGATTCTAACTCCTGATTTTGTTTCGGCTCATCCGAATCGCATCATCAATATCCACCATTCATTCCTGCCAG
CTTTTATCGGTGCGAATCCGTACAAACGGGCCTACGAGCGCGGCGTGAAACTGATCGGTGCGACATCTCATTATGTGACA
AACGATCTTGATGAAGGGCCGATCATTGAACAGGATATTGAGCGTGTGGACCACCGCGATAATGCGGAAGCGCTGAAAAA
CATCGGAAGAACAATTGAGCGCAGCGTGCTTGCCCGTGCTGTGAAATGGCATTTGGAAGACCGTGTCATCGTTCATGAAA
ATAAAACAATCGTCTTTAACTAG

Upstream 100 bases:

>100_bases
TCAGCAGTTGGTTTAAAAATTCTGTCATAAATTGATTTTTATCAAATCTTCAGTATAATGGATAAATAGTTTAGACTTAC
AAAGATAAGAGGGATTATTC

Downstream 100 bases:

>100_bases
ACTGCAAGAGGCCCGCGCAATGCGGGCTATTTTTGATGACAAAAAACCTTGACAAGTGTCTTTTTTCTTTGCATAATATA
AAAAAATCATTGAGCGTTGA

Product: formyltetrahydrofolate deformylase

Products: NA

Alternate protein names: Formyl-FH(4) hydrolase

Number of amino acids: Translated: 300; Mature: 300

Protein sequence:

>300_residues
MKSYMTQRLDEYRDGNEDKGRLLVSCPDQPGIVSAVSAFLFEHGANIIESNQYTTDPEGGRFFLRIEFDCAGIREKKSSL
QAAFASVAEKFDMTWSLTLASELKRVAIFVSKELHCLHELIWEWQTGNLMAEIAVVISNHEEARELVERLNIPFHYMKAN
KDIRAEVEKKQLELLEQYDVDVIVLARYMQILTPDFVSAHPNRIINIHHSFLPAFIGANPYKRAYERGVKLIGATSHYVT
NDLDEGPIIEQDIERVDHRDNAEALKNIGRTIERSVLARAVKWHLEDRVIVHENKTIVFN

Sequences:

>Translated_300_residues
MKSYMTQRLDEYRDGNEDKGRLLVSCPDQPGIVSAVSAFLFEHGANIIESNQYTTDPEGGRFFLRIEFDCAGIREKKSSL
QAAFASVAEKFDMTWSLTLASELKRVAIFVSKELHCLHELIWEWQTGNLMAEIAVVISNHEEARELVERLNIPFHYMKAN
KDIRAEVEKKQLELLEQYDVDVIVLARYMQILTPDFVSAHPNRIINIHHSFLPAFIGANPYKRAYERGVKLIGATSHYVT
NDLDEGPIIEQDIERVDHRDNAEALKNIGRTIERSVLARAVKWHLEDRVIVHENKTIVFN
>Mature_300_residues
MKSYMTQRLDEYRDGNEDKGRLLVSCPDQPGIVSAVSAFLFEHGANIIESNQYTTDPEGGRFFLRIEFDCAGIREKKSSL
QAAFASVAEKFDMTWSLTLASELKRVAIFVSKELHCLHELIWEWQTGNLMAEIAVVISNHEEARELVERLNIPFHYMKAN
KDIRAEVEKKQLELLEQYDVDVIVLARYMQILTPDFVSAHPNRIINIHHSFLPAFIGANPYKRAYERGVKLIGATSHYVT
NDLDEGPIIEQDIERVDHRDNAEALKNIGRTIERSVLARAVKWHLEDRVIVHENKTIVFN

Specific function: Produces Formate From Formyl-Tetrahydrofolate. Provides The Major Source Of Formate For The Purt-Dependent Synthesis Of 5'-Phosphoribosyl-N-Formylglycinamide (Fgar) During Aerobic Growth. Has A Role In Regulating The One-Carbon Pool. [C]

COG id: COG0788

COG function: function code F; Formyltetrahydrofolate hydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ACT domain

Homologues:

Organism=Homo sapiens, GI4503915, Length=221, Percent_Identity=30.7692307692308, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI209869995, Length=221, Percent_Identity=30.7692307692308, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI209869993, Length=221, Percent_Identity=30.7692307692308, Blast_Score=100, Evalue=2e-21,
Organism=Escherichia coli, GI1787483, Length=274, Percent_Identity=45.2554744525547, Blast_Score=242, Evalue=2e-65,
Organism=Escherichia coli, GI1788846, Length=192, Percent_Identity=29.6875, Blast_Score=94, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17567511, Length=164, Percent_Identity=34.1463414634146, Blast_Score=97, Evalue=7e-21,
Organism=Drosophila melanogaster, GI24582400, Length=188, Percent_Identity=31.3829787234043, Blast_Score=69, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PURU_BACSU (O34990)

Other databases:

- EMBL:   AJ002571
- EMBL:   AL009126
- PIR:   C69857
- RefSeq:   NP_389194.2
- HSSP:   P22102
- ProteinModelPortal:   O34990
- SMR:   O34990
- EnsemblBacteria:   EBBACT00000001307
- GeneID:   939854
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU13110
- NMPDR:   fig|224308.1.peg.1313
- GenoList:   BSU13110
- GeneTree:   EBGT00050000001190
- HOGENOM:   HBG286016
- ProtClustDB:   PRK06027
- BioCyc:   BSUB:BSU13110-MONOMER
- InterPro:   IPR002912
- InterPro:   IPR002376
- InterPro:   IPR004810
- Gene3D:   G3DSA:3.40.50.170
- PIRSF:   PIRSF036480
- PRINTS:   PR01575
- TIGRFAMs:   TIGR00655

Pfam domain/function: PF01842 ACT; PF00551 Formyl_trans_N; SSF53328 formyl_transf

EC number: =3.5.1.10

Molecular weight: Translated: 34478; Mature: 34478

Theoretical pI: Translated: 5.93; Mature: 5.93

Prosite motif: NA

Important sites: ACT_SITE 244-244

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSYMTQRLDEYRDGNEDKGRLLVSCPDQPGIVSAVSAFLFEHGANIIESNQYTTDPEGG
CCHHHHHHHHHHCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCEECCCCCCCCCCC
RFFLRIEFDCAGIREKKSSLQAAFASVAEKFDMTWSLTLASELKRVAIFVSKELHCLHEL
EEEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCEEEEHHHHHHHHHHHHHHHHHHHHHHH
IWEWQTGNLMAEIAVVISNHEEARELVERLNIPFHYMKANKDIRAEVEKKQLELLEQYDV
HHHCCCCCHHHHHHHHHCCCHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHCCC
DVIVLARYMQILTPDFVSAHPNRIINIHHSFLPAFIGANPYKRAYERGVKLIGATSHYVT
CHHHHHHHHHHHCCHHHCCCCCCEEEEHHHHHHHHHCCCHHHHHHHCCCEEEECCHHHHC
NDLDEGPIIEQDIERVDHRDNAEALKNIGRTIERSVLARAVKWHLEDRVIVHENKTIVFN
CCCCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCEEEEC
>Mature Secondary Structure
MKSYMTQRLDEYRDGNEDKGRLLVSCPDQPGIVSAVSAFLFEHGANIIESNQYTTDPEGG
CCHHHHHHHHHHCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCEECCCCCCCCCCC
RFFLRIEFDCAGIREKKSSLQAAFASVAEKFDMTWSLTLASELKRVAIFVSKELHCLHEL
EEEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCEEEEHHHHHHHHHHHHHHHHHHHHHHH
IWEWQTGNLMAEIAVVISNHEEARELVERLNIPFHYMKANKDIRAEVEKKQLELLEQYDV
HHHCCCCCHHHHHHHHHCCCHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHCCC
DVIVLARYMQILTPDFVSAHPNRIINIHHSFLPAFIGANPYKRAYERGVKLIGATSHYVT
CHHHHHHHHHHHCCHHHCCCCCCEEEEHHHHHHHHHCCCHHHHHHHCCCEEEECCHHHHC
NDLDEGPIIEQDIERVDHRDNAEALKNIGRTIERSVLARAVKWHLEDRVIVHENKTIVFN
CCCCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377