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The map label for this gene is lytF

Identifier: 255767220

GI number: 255767220

Start: 1011792

End: 1013258

Strand: Reverse

Name: lytF

Synonym: BSU09370

Alternate gene names: 255767220

Gene position: 1013258-1011792 (Counterclockwise)

Preceding gene: 16078003

Following gene: 16078000

Centisome position: 24.04

GC content: 42.88

Gene sequence:

>1467_bases
ATGAAAAAGAAATTAGCAGCAGGGCTGACAGCATCTGCGATTGTCGGCACAACTTTAGTAGTGACACCAGCTGAAGCAGC
AACGATTAAGGTCAAAAGCGGAGATTCTTTATGGAAACTGGCCCAAACCTATAACACAAGTGTGGCGGCCCTCACATCCG
CCAATCATCTTTCGACTACTGTTCTGTCGATCGGCCAGACGCTTACTATTCCAGGCAGCAAATCCAGCACTTCTTCATCT
ACTTCAAGCTCAACAACCAAGAAGAGCGGAAGTTCTGTTTACACAGTGAAAAGCGGAGATTCACTTTGGCTGATCGCAAA
TGAGTTTAAAATGACGGTGCAGGAACTGAAAAAATTAAACGGACTAAGCAGCGATTTAATTCGTGCTGGGCAGAAATTAA
AGGTGTCCGGTACTGTTTCTTCAAGCTCTAGTTCCAGTAAAAAAAGCAACTCTAATAAGAGTTCAAGCTCCAGTTCAAAA
AGCAGTTCTAATAAGAGTTCAAGTTCTTCATCTTCTACGGGAACATATAAGGTGCAGCTTGGAGATTCACTTTGGAAAAT
TGCAAACAAAGTCAATATGTCCATCGCTGAATTGAAGGTCTTGAACAACTTAAAATCAGACACCATTTACGTGAATCAGG
TGTTGAAAACAAAATCAAGCGGCTCTGATACGTCTTCTAAAGACAATTCATCCAAATCGAACCAAACTTCAGCAACAACT
AAATATACCGTTAAAAGCGGTGATTCACTTTGGAAAATCGCAAACAACTATAACCTGACTGTACAGCAAATCCGAAATAT
CAACAATCTGAAATCAGATGTGCTTTACGTTGGACAAGTATTGAAGCTGACAGGTAAAGCATCTTCAGGCTCTTCATCAT
CGTCGTCTTCTTCGTCAAATGCAAGCTCCGGCACGACGACTACATACACGGTGAAAAGCGGTGATTCCTTATGGGTGATT
GCTCAAAAATTTAATGTAACAGCTCAGCAGATTCGTGAGAAAAACAATTTAAAAACGGATGTCTTGCAGGTTGGCCAAAA
GCTGGTCATTTCCGGAAAAGCTTCATCATCGTCTTCATCCGGTTCTTCAAACACCACCAGCTCAACGAGTGCGAAGATTA
ACACAATGATTTCGGCCGCTAAAGCGCAGCTTGGGGTTCCGTATCGCTGGGGAGGCACGACACCGTCCGGGTTTGACTGC
AGCGGATTCATTTACTATGTACTGAACAAAGTCACATCCGTATCTAGATTAACGGCAGCGGGATATTGGAACACAATGAA
ATCAGTTAGCCAGCCTGCTGTCGGAGATTTCGTTTTCTTTTCAACATATAAAGCCGGCCCTTCCCACGTCGGAATTTACC
TAGGCAACGGGGAATTCATAAATGCCAACGATTCCGGAGTTGTCATTTCCAATATGAACAACTCTTACTGGAAACAGCGC
TATCTCGGTGCAAAACGATATTTCTAA

Upstream 100 bases:

>100_bases
TTTGGCTATTTTTATAAAAAGGCAAAACGATGAGACAAATGTGCCGATATAAAAAAGAAACAGCAAATGCAAAATCATTG
GGGGAAAAAGGAGAGCTAAG

Downstream 100 bases:

>100_bases
AAACAGAAACTGTGCGGCCTTAACGGCTGTACAGTTTTTTATTAGAGCCTAAAGACGTTGATGCTGATCATATGATTTGT
CACCGGATGAAAATAGACTT

Product: gamma-D-glutamate-meso-diaminopimelate muropeptidase

Products: NA

Alternate protein names: Cell wall-associated polypeptide CWBP49; Gamma-D-glutamate-meso-diaminopimelate muropeptidase lytF

Number of amino acids: Translated: 488; Mature: 488

Protein sequence:

>488_residues
MKKKLAAGLTASAIVGTTLVVTPAEAATIKVKSGDSLWKLAQTYNTSVAALTSANHLSTTVLSIGQTLTIPGSKSSTSSS
TSSSTTKKSGSSVYTVKSGDSLWLIANEFKMTVQELKKLNGLSSDLIRAGQKLKVSGTVSSSSSSSKKSNSNKSSSSSSK
SSSNKSSSSSSSTGTYKVQLGDSLWKIANKVNMSIAELKVLNNLKSDTIYVNQVLKTKSSGSDTSSKDNSSKSNQTSATT
KYTVKSGDSLWKIANNYNLTVQQIRNINNLKSDVLYVGQVLKLTGKASSGSSSSSSSSSNASSGTTTTYTVKSGDSLWVI
AQKFNVTAQQIREKNNLKTDVLQVGQKLVISGKASSSSSSGSSNTTSSTSAKINTMISAAKAQLGVPYRWGGTTPSGFDC
SGFIYYVLNKVTSVSRLTAAGYWNTMKSVSQPAVGDFVFFSTYKAGPSHVGIYLGNGEFINANDSGVVISNMNNSYWKQR
YLGAKRYF

Sequences:

>Translated_488_residues
MKKKLAAGLTASAIVGTTLVVTPAEAATIKVKSGDSLWKLAQTYNTSVAALTSANHLSTTVLSIGQTLTIPGSKSSTSSS
TSSSTTKKSGSSVYTVKSGDSLWLIANEFKMTVQELKKLNGLSSDLIRAGQKLKVSGTVSSSSSSSKKSNSNKSSSSSSK
SSSNKSSSSSSSTGTYKVQLGDSLWKIANKVNMSIAELKVLNNLKSDTIYVNQVLKTKSSGSDTSSKDNSSKSNQTSATT
KYTVKSGDSLWKIANNYNLTVQQIRNINNLKSDVLYVGQVLKLTGKASSGSSSSSSSSSNASSGTTTTYTVKSGDSLWVI
AQKFNVTAQQIREKNNLKTDVLQVGQKLVISGKASSSSSSGSSNTTSSTSAKINTMISAAKAQLGVPYRWGGTTPSGFDC
SGFIYYVLNKVTSVSRLTAAGYWNTMKSVSQPAVGDFVFFSTYKAGPSHVGIYLGNGEFINANDSGVVISNMNNSYWKQR
YLGAKRYF
>Mature_488_residues
MKKKLAAGLTASAIVGTTLVVTPAEAATIKVKSGDSLWKLAQTYNTSVAALTSANHLSTTVLSIGQTLTIPGSKSSTSSS
TSSSTTKKSGSSVYTVKSGDSLWLIANEFKMTVQELKKLNGLSSDLIRAGQKLKVSGTVSSSSSSSKKSNSNKSSSSSSK
SSSNKSSSSSSSTGTYKVQLGDSLWKIANKVNMSIAELKVLNNLKSDTIYVNQVLKTKSSGSDTSSKDNSSKSNQTSATT
KYTVKSGDSLWKIANNYNLTVQQIRNINNLKSDVLYVGQVLKLTGKASSGSSSSSSSSSNASSGTTTTYTVKSGDSLWVI
AQKFNVTAQQIREKNNLKTDVLQVGQKLVISGKASSSSSSGSSNTTSSTSAKINTMISAAKAQLGVPYRWGGTTPSGFDC
SGFIYYVLNKVTSVSRLTAAGYWNTMKSVSQPAVGDFVFFSTYKAGPSHVGIYLGNGEFINANDSGVVISNMNNSYWKQR
YLGAKRYF

Specific function: Cleaves gamma-D-glutamate-meso-diaminopimelate bonds. Cell wall hydrolase involved in cell autolysis

COG id: COG1388

COG function: function code M; FOG: LysM repeat

Gene ontology:

Cell location: Secreted, cell wall. Note=LysM repeats are thought to be involved in peptidoglycan binding

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 5 LysM repeats

Homologues:

Organism=Escherichia coli, GI1787944, Length=113, Percent_Identity=38.0530973451327, Blast_Score=96, Evalue=6e-21,
Organism=Escherichia coli, GI1786421, Length=122, Percent_Identity=35.2459016393443, Blast_Score=89, Evalue=5e-19,
Organism=Escherichia coli, GI1788501, Length=106, Percent_Identity=43.3962264150943, Blast_Score=81, Evalue=2e-16,
Organism=Escherichia coli, GI1788001, Length=121, Percent_Identity=34.7107438016529, Blast_Score=62, Evalue=8e-11,
Organism=Caenorhabditis elegans, GI17559362, Length=350, Percent_Identity=25.1428571428571, Blast_Score=75, Evalue=7e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LYTF_BACSU (O07532)

Other databases:

- EMBL:   Y14079
- EMBL:   AL009126
- PIR:   B69825
- RefSeq:   NP_388818.2
- ProteinModelPortal:   O07532
- MEROPS:   C40.002
- EnsemblBacteria:   EBBACT00000003797
- GeneID:   939271
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU09370
- NMPDR:   fig|224308.1.peg.937
- GenoList:   BSU09370
- GeneTree:   EBGT00070000031872
- HOGENOM:   HBG362075
- ProtClustDB:   CLSK886953
- BioCyc:   BSUB:BSU09370-MONOMER
- InterPro:   IPR000064
- InterPro:   IPR018392
- InterPro:   IPR002482
- SMART:   SM00257

Pfam domain/function: PF01476 LysM; PF00877 NLPC_P60

EC number: NA

Molecular weight: Translated: 51395; Mature: 51395

Theoretical pI: Translated: 10.64; Mature: 10.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKKLAAGLTASAIVGTTLVVTPAEAATIKVKSGDSLWKLAQTYNTSVAALTSANHLSTT
CCCCHHCCCCHHHHHCEEEEEECCCCEEEEEECCCHHHHHHHHCCCCEEEEECCCHHHHH
VLSIGQTLTIPGSKSSTSSSTSSSTTKKSGSSVYTVKSGDSLWLIANEFKMTVQELKKLN
HHHCCCEEECCCCCCCCCCCCCCCCCCCCCCEEEEEECCCEEEEEEHHHHHHHHHHHHHC
GLSSDLIRAGQKLKVSGTVSSSSSSSKKSNSNKSSSSSSKSSSNKSSSSSSSTGTYKVQL
CCCHHHHHCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEE
GDSLWKIANKVNMSIAELKVLNNLKSDTIYVNQVLKTKSSGSDTSSKDNSSKSNQTSATT
CHHHHHHHHHHCCHHHHHHHHHCCCCCEEEEEHHHHCCCCCCCCCCCCCCCCCCCCCCEE
KYTVKSGDSLWKIANNYNLTVQQIRNINNLKSDVLYVGQVLKLTGKASSGSSSSSSSSSN
EEEEECCCCEEEEECCCCEEHHHHHCHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCC
ASSGTTTTYTVKSGDSLWVIAQKFNVTAQQIREKNNLKTDVLQVGQKLVISGKASSSSSS
CCCCCEEEEEEECCCEEEEEEEECCCHHHHHHHHCCCHHHHHHCCCEEEEECCCCCCCCC
GSSNTTSSTSAKINTMISAAKAQLGVPYRWGGTTPSGFDCSGFIYYVLNKVTSVSRLTAA
CCCCCCCCCCHHHHHHHHHHHHHCCCCEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
GYWNTMKSVSQPAVGDFVFFSTYKAGPSHVGIYLGNGEFINANDSGVVISNMNNSYWKQR
HHHHHHHHHCCCCCCCEEEEEEECCCCCEEEEEECCCCEECCCCCEEEEECCCCHHHHHH
YLGAKRYF
HHHHHCCC
>Mature Secondary Structure
MKKKLAAGLTASAIVGTTLVVTPAEAATIKVKSGDSLWKLAQTYNTSVAALTSANHLSTT
CCCCHHCCCCHHHHHCEEEEEECCCCEEEEEECCCHHHHHHHHCCCCEEEEECCCHHHHH
VLSIGQTLTIPGSKSSTSSSTSSSTTKKSGSSVYTVKSGDSLWLIANEFKMTVQELKKLN
HHHCCCEEECCCCCCCCCCCCCCCCCCCCCCEEEEEECCCEEEEEEHHHHHHHHHHHHHC
GLSSDLIRAGQKLKVSGTVSSSSSSSKKSNSNKSSSSSSKSSSNKSSSSSSSTGTYKVQL
CCCHHHHHCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEE
GDSLWKIANKVNMSIAELKVLNNLKSDTIYVNQVLKTKSSGSDTSSKDNSSKSNQTSATT
CHHHHHHHHHHCCHHHHHHHHHCCCCCEEEEEHHHHCCCCCCCCCCCCCCCCCCCCCCEE
KYTVKSGDSLWKIANNYNLTVQQIRNINNLKSDVLYVGQVLKLTGKASSGSSSSSSSSSN
EEEEECCCCEEEEECCCCEEHHHHHCHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCC
ASSGTTTTYTVKSGDSLWVIAQKFNVTAQQIREKNNLKTDVLQVGQKLVISGKASSSSSS
CCCCCEEEEEEECCCEEEEEEEECCCHHHHHHHHCCCHHHHHHCCCEEEEECCCCCCCCC
GSSNTTSSTSAKINTMISAAKAQLGVPYRWGGTTPSGFDCSGFIYYVLNKVTSVSRLTAA
CCCCCCCCCCHHHHHHHHHHHHHCCCCEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
GYWNTMKSVSQPAVGDFVFFSTYKAGPSHVGIYLGNGEFINANDSGVVISNMNNSYWKQR
HHHHHHHHHCCCCCCCEEEEEEECCCCCEEEEEECCCCEECCCCCEEEEECCCCHHHHHH
YLGAKRYF
HHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377; 10206711