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The map label for this gene is yfkA

Identifier: 255767189

GI number: 255767189

Start: 868007

End: 869128

Strand: Reverse

Name: yfkA

Synonym: BSU07955

Alternate gene names: 255767189

Gene position: 869128-868007 (Counterclockwise)

Preceding gene: 255767190

Following gene: 16077861

Centisome position: 20.62

GC content: 48.75

Gene sequence:

>1122_bases
ATGACACAAAACACGAAGCTGCGTCCGATTACGCCCGAGTTTGACCCTTGGGAAGCCTATATGGATGTTGACCAATACGG
AGACATGCAGCTGACAAACGTTGAATTCACAACAACGACACTGTGCAATATGAGATGCGAGCATTGTGCTGTAGGCTATA
CATTGCAGCCTAAAGACCCGAATGCTCTGCCAATAGATCTTCTGTTAAAACGGCTGGAGGAAATCCCGCGCCTCAGATCC
ATCAGCATCACAGGCGGAGAGCCGATGCTTTCGTTGAAATCAGTAAAAGAATATGTTGTTCCTTTATTGAAATATGCCCA
TGAACGGGGTGTGCGGACACAAATTAATTCAAATCTGACCCTCGACATCGAGCGGTACGAATGGATCATTCCGTATCTCG
ATGTGCTACATATTTCACATAACTGGGGAACAGTTGAAGATTTTGCTGAAATCGGCTTTGCCATGATGGACAGAAAACCG
ACCTTCGAACAGCGCGCGCGCTATTTTGAAAAAATGATCGAAAACAGCCGCACGCTCGTGGATGCCGGAGTAATGGTATC
TGCGGAAACGATGCTGAACAAACGAACACTTCCGCATATTGAGCATATTCACCGCCAAATCACAGAAGATATGAAATGTC
AGCGTCACGAGGTCCACCCAATGTATCCAAGCGATTTCGCAAGCGCTCTTGAATCCCTCAGCCTGAAAGAGATGAGACAG
GCCATTCACCGCTTGCTGGATATTCGTGATGAAAATACGTGGATGCTGTTCGGAACGCTGCCGTTTTATGCCTGCAGTCC
GGACGAAGACGACCAAAAGCTGCTTCGCCGGCTTCGCGCGGCGAAAAACGTCACAGTGCGAAATGACCCTGACGGCCGAT
CCCGCCTGAATGTGAACATTTTCGACGGAAACATTATCGTGACCGATTTCGGTGATACCCCGCCGCTTGGCAACATTCAG
ACGGACAGCCTCCCTTCCGCCTATGAAAAGTGGAGAGAGACCAAGCTTGCGAAAGAACTGAGTTGCCACTGCCCGAATGT
CCGGTGCCTCGGACCGAATGTTCTTGTCAAAAACAGTTATTATCAGGATACAGACTTCACAAAACGTCAAGCCAGGAGTT
AG

Upstream 100 bases:

>100_bases
CGTTTTTCAATCGCCGCGCTACTTGCGATCTTGGCAAATCAGGTCTAAAATTAACTGTTGGCAGCGTTAGTATGCCCATT
TATAAAGGAGTTAGATAGTC

Downstream 100 bases:

>100_bases
ATGCGGATGAAAGAAACACTTACGGAGATTTTTCAAAATAAAATCGTCGATATTCTCCTCGTTGCCGTTATCCTTTGGAT
CGGTGTCTTTATCATCAACC

Product: Fe-S oxidoreductase, radical SAM superfamily

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 373; Mature: 372

Protein sequence:

>373_residues
MTQNTKLRPITPEFDPWEAYMDVDQYGDMQLTNVEFTTTTLCNMRCEHCAVGYTLQPKDPNALPIDLLLKRLEEIPRLRS
ISITGGEPMLSLKSVKEYVVPLLKYAHERGVRTQINSNLTLDIERYEWIIPYLDVLHISHNWGTVEDFAEIGFAMMDRKP
TFEQRARYFEKMIENSRTLVDAGVMVSAETMLNKRTLPHIEHIHRQITEDMKCQRHEVHPMYPSDFASALESLSLKEMRQ
AIHRLLDIRDENTWMLFGTLPFYACSPDEDDQKLLRRLRAAKNVTVRNDPDGRSRLNVNIFDGNIIVTDFGDTPPLGNIQ
TDSLPSAYEKWRETKLAKELSCHCPNVRCLGPNVLVKNSYYQDTDFTKRQARS

Sequences:

>Translated_373_residues
MTQNTKLRPITPEFDPWEAYMDVDQYGDMQLTNVEFTTTTLCNMRCEHCAVGYTLQPKDPNALPIDLLLKRLEEIPRLRS
ISITGGEPMLSLKSVKEYVVPLLKYAHERGVRTQINSNLTLDIERYEWIIPYLDVLHISHNWGTVEDFAEIGFAMMDRKP
TFEQRARYFEKMIENSRTLVDAGVMVSAETMLNKRTLPHIEHIHRQITEDMKCQRHEVHPMYPSDFASALESLSLKEMRQ
AIHRLLDIRDENTWMLFGTLPFYACSPDEDDQKLLRRLRAAKNVTVRNDPDGRSRLNVNIFDGNIIVTDFGDTPPLGNIQ
TDSLPSAYEKWRETKLAKELSCHCPNVRCLGPNVLVKNSYYQDTDFTKRQARS
>Mature_372_residues
TQNTKLRPITPEFDPWEAYMDVDQYGDMQLTNVEFTTTTLCNMRCEHCAVGYTLQPKDPNALPIDLLLKRLEEIPRLRSI
SITGGEPMLSLKSVKEYVVPLLKYAHERGVRTQINSNLTLDIERYEWIIPYLDVLHISHNWGTVEDFAEIGFAMMDRKPT
FEQRARYFEKMIENSRTLVDAGVMVSAETMLNKRTLPHIEHIHRQITEDMKCQRHEVHPMYPSDFASALESLSLKEMRQA
IHRLLDIRDENTWMLFGTLPFYACSPDEDDQKLLRRLRAAKNVTVRNDPDGRSRLNVNIFDGNIIVTDFGDTPPLGNIQT
DSLPSAYEKWRETKLAKELSCHCPNVRCLGPNVLVKNSYYQDTDFTKRQARS

Specific function: Unknown

COG id: COG0535

COG function: function code R; Predicted Fe-S oxidoreductases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): YFKA_BACSU (O34400)

Other databases:

- EMBL:   D83967
- EMBL:   D83967
- EMBL:   AL009126
- PIR:   F69807
- RefSeq:   NP_388677.2
- ProteinModelPortal:   O34400
- EnsemblBacteria:   EBBACT00000082638
- GeneID:   938812
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU07955
- GenoList:   BSU07955
- GeneTree:   EBGT00050000001240
- ProtClustDB:   CLSK872858
- BioCyc:   BSUB:BSU07955-MONOMER
- InterPro:   IPR007197
- InterPro:   IPR014866

Pfam domain/function: PF04055 Radical_SAM; PF08756 YfkB

EC number: NA

Molecular weight: Translated: 43258; Mature: 43127

Theoretical pI: Translated: 6.29; Mature: 6.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQNTKLRPITPEFDPWEAYMDVDQYGDMQLTNVEFTTTTLCNMRCEHCAVGYTLQPKDP
CCCCCCCCCCCCCCCCHHHHCCHHHCCCEEEEEEEEEHHHHHHCCHHHEEECCEECCCCC
NALPIDLLLKRLEEIPRLRSISITGGEPMLSLKSVKEYVVPLLKYAHERGVRTQINSNLT
CCCCHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEECCCEE
LDIERYEWIIPYLDVLHISHNWGTVEDFAEIGFAMMDRKPTFEQRARYFEKMIENSRTLV
EEEHHHHHHHHHHHHEEEECCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCEEE
DAGVMVSAETMLNKRTLPHIEHIHRQITEDMKCQRHEVHPMYPSDFASALESLSLKEMRQ
ECCCEEEHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCHHHHHH
AIHRLLDIRDENTWMLFGTLPFYACSPDEDDQKLLRRLRAAKNVTVRNDPDGRSRLNVNI
HHHHHHCCCCCCEEEEEECCCEEECCCCCHHHHHHHHHHHHCCCEECCCCCCCCEEEEEE
FDGNIIVTDFGDTPPLGNIQTDSLPSAYEKWRETKLAKELSCHCPNVRCLGPNVLVKNSY
EECEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEECCCEEEECCC
YQDTDFTKRQARS
CCCCCHHHHHCCC
>Mature Secondary Structure 
TQNTKLRPITPEFDPWEAYMDVDQYGDMQLTNVEFTTTTLCNMRCEHCAVGYTLQPKDP
CCCCCCCCCCCCCCCHHHHCCHHHCCCEEEEEEEEEHHHHHHCCHHHEEECCEECCCCC
NALPIDLLLKRLEEIPRLRSISITGGEPMLSLKSVKEYVVPLLKYAHERGVRTQINSNLT
CCCCHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEECCCEE
LDIERYEWIIPYLDVLHISHNWGTVEDFAEIGFAMMDRKPTFEQRARYFEKMIENSRTLV
EEEHHHHHHHHHHHHEEEECCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCEEE
DAGVMVSAETMLNKRTLPHIEHIHRQITEDMKCQRHEVHPMYPSDFASALESLSLKEMRQ
ECCCEEEHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCHHHHHH
AIHRLLDIRDENTWMLFGTLPFYACSPDEDDQKLLRRLRAAKNVTVRNDPDGRSRLNVNI
HHHHHHCCCCCCEEEEEECCCEEECCCCCHHHHHHHHHHHHCCCEECCCCCCCCEEEEEE
FDGNIIVTDFGDTPPLGNIQTDSLPSAYEKWRETKLAKELSCHCPNVRCLGPNVLVKNSY
EECEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEECCCEEEECCC
YQDTDFTKRQARS
CCCCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969503; 9384377