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The map label for this gene is yfjR

Identifier: 255767190

GI number: 255767190

Start: 870388

End: 871248

Strand: Reverse

Name: yfjR

Synonym: BSU07990

Alternate gene names: 255767190

Gene position: 871248-870388 (Counterclockwise)

Preceding gene: 16077867

Following gene: 255767189

Centisome position: 20.67

GC content: 49.83

Gene sequence:

>861_bases
TTGAAAATTGCTGTCATCGGACTCGGCAATATGGGACAGCCCATTGCCCGAAATGTTCTTCAAGCAGGCTACGAATTGAC
TGTCTATAACCGGACGAAACAAAAGACAGAAGACCTTGTCACAGAAGGCGCACAGGCAGCTGATACGCCGCGGCTGGCGG
CAAAGTCCGCTGATATTGTCATCACAATGCTTGCAGATGATGATTCTGTCAGCACCGTGACATTCGGAGAAGACGGGCTG
CTTGAAGGATTAGCAGAGAACGGCATACACATCTCGATGAGCACAATCAGTGTTGAGTTCTCAGAAAAGCTCGCAGCGGC
TCATGCAGAGAAAGGACAATTTTTTCTCGCCGCTCCTGTCCTTGGCAGACCGGATGCCGCAGCCAAAGCCGCGCTCCGTA
TCATAACAGCCGGACCGGCGGAAGCAAAGCAAGCTGCCAAGCCTCTGCTCGACAGCCTGAGCCAGCAGATATTTGACGTC
GGCGAAGAAAGCAAGACGGCAAATGCAGCCAAAATCAGCATTAATTTCTTGCTTGTGTCCATGCTGGAGGCGTTATCTGA
ATCCTTTTTAATGATGGAGAAATACGGCTTAGAACAAAAACAATTTTTAGAAATCGCCAGCGCGCTCTTTGGTTCTCCAG
TCTATCAAAATTACGGAACCATTATGGCCGAGCAGAAATTTGAGCCGGCCGGCTTCAAAATGTCTTTAGGGCTGAAGGAT
ACCAACCTGGCACTCGCCGCCGCAAAACGGGTTTCTGCAAATCTTCCTCTTGCCGAGCTGGCCAAGAGCCATTTTGAAAG
CGGGATTGAGAAAGGCTTCGGGGATCTGGACTGGGCCGCACTGATTAAATGTATAAAATAA

Upstream 100 bases:

>100_bases
GAGGACAGCGAGCAGCTGTTCTTTTTTGTTGTAAGCCGATCGCTATGCATATTCTGTTCATATTGTTTATAATGGAAAAA
ATATTCAGGGAGTGATTAGA

Downstream 100 bases:

>100_bases
AGAAAAGCCTCTCCGTTTAAGGAGAGGTCTTTCTCTTTTACAAAGACGGCAGCCTCATTTCTTTCTCAAACATCAGATCA
TCAATGCTTTTAAAAGTGTA

Product: beta-hydroxyacid dehydrogenase

Products: 2-methyl-3-oxopropanoate; NADH; H+

Alternate protein names: NA

Number of amino acids: Translated: 286; Mature: 286

Protein sequence:

>286_residues
MKIAVIGLGNMGQPIARNVLQAGYELTVYNRTKQKTEDLVTEGAQAADTPRLAAKSADIVITMLADDDSVSTVTFGEDGL
LEGLAENGIHISMSTISVEFSEKLAAAHAEKGQFFLAAPVLGRPDAAAKAALRIITAGPAEAKQAAKPLLDSLSQQIFDV
GEESKTANAAKISINFLLVSMLEALSESFLMMEKYGLEQKQFLEIASALFGSPVYQNYGTIMAEQKFEPAGFKMSLGLKD
TNLALAAAKRVSANLPLAELAKSHFESGIEKGFGDLDWAALIKCIK

Sequences:

>Translated_286_residues
MKIAVIGLGNMGQPIARNVLQAGYELTVYNRTKQKTEDLVTEGAQAADTPRLAAKSADIVITMLADDDSVSTVTFGEDGL
LEGLAENGIHISMSTISVEFSEKLAAAHAEKGQFFLAAPVLGRPDAAAKAALRIITAGPAEAKQAAKPLLDSLSQQIFDV
GEESKTANAAKISINFLLVSMLEALSESFLMMEKYGLEQKQFLEIASALFGSPVYQNYGTIMAEQKFEPAGFKMSLGLKD
TNLALAAAKRVSANLPLAELAKSHFESGIEKGFGDLDWAALIKCIK
>Mature_286_residues
MKIAVIGLGNMGQPIARNVLQAGYELTVYNRTKQKTEDLVTEGAQAADTPRLAAKSADIVITMLADDDSVSTVTFGEDGL
LEGLAENGIHISMSTISVEFSEKLAAAHAEKGQFFLAAPVLGRPDAAAKAALRIITAGPAEAKQAAKPLLDSLSQQIFDV
GEESKTANAAKISINFLLVSMLEALSESFLMMEKYGLEQKQFLEIASALFGSPVYQNYGTIMAEQKFEPAGFKMSLGLKD
TNLALAAAKRVSANLPLAELAKSHFESGIEKGFGDLDWAALIKCIK

Specific function: D-galactarate metabolism; third step. [C]

COG id: COG2084

COG function: function code I; 3-hydroxyisobutyrate dehydrogenase and related beta-hydroxyacid dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 3-hydroxyisobutyrate dehydrogenase family

Homologues:

Organism=Homo sapiens, GI40556376, Length=289, Percent_Identity=26.643598615917, Blast_Score=100, Evalue=1e-21,
Organism=Homo sapiens, GI23308751, Length=292, Percent_Identity=27.3972602739726, Blast_Score=100, Evalue=2e-21,
Organism=Escherichia coli, GI145693186, Length=282, Percent_Identity=30.1418439716312, Blast_Score=126, Evalue=2e-30,
Organism=Escherichia coli, GI1786719, Length=295, Percent_Identity=31.5254237288136, Blast_Score=123, Evalue=1e-29,
Organism=Escherichia coli, GI1790315, Length=290, Percent_Identity=30.6896551724138, Blast_Score=123, Evalue=1e-29,
Organism=Escherichia coli, GI1789092, Length=284, Percent_Identity=23.943661971831, Blast_Score=62, Evalue=5e-11,
Organism=Caenorhabditis elegans, GI17557316, Length=269, Percent_Identity=28.2527881040892, Blast_Score=75, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24655230, Length=292, Percent_Identity=28.0821917808219, Blast_Score=106, Evalue=1e-23,
Organism=Drosophila melanogaster, GI19922568, Length=292, Percent_Identity=28.0821917808219, Blast_Score=106, Evalue=1e-23,
Organism=Drosophila melanogaster, GI28574115, Length=281, Percent_Identity=23.4875444839858, Blast_Score=85, Evalue=4e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): YFJR_BACSU (O34969)

Other databases:

- EMBL:   D83967
- EMBL:   D78509
- EMBL:   AL009126
- PIR:   A69807
- RefSeq:   NP_388680.2
- ProteinModelPortal:   O34969
- SMR:   O34969
- EnsemblBacteria:   EBBACT00000002573
- GeneID:   939198
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU07990
- NMPDR:   fig|224308.1.peg.799
- GenoList:   BSU07990
- GeneTree:   EBGT00050000001992
- HOGENOM:   HBG729179
- ProtClustDB:   CLSK886877
- BioCyc:   BSUB:BSU07990-MONOMER
- InterPro:   IPR015815
- InterPro:   IPR008927
- InterPro:   IPR006115
- InterPro:   IPR013328
- InterPro:   IPR016040
- Gene3D:   G3DSA:3.40.50.720
- Gene3D:   G3DSA:1.10.1040.10
- PANTHER:   PTHR22981

Pfam domain/function: PF03446 NAD_binding_2; SSF48179 6DGDH_C_like

EC number: 1.1.1.31

Molecular weight: Translated: 30474; Mature: 30474

Theoretical pI: Translated: 4.81; Mature: 4.81

Prosite motif: PS00895 3_HYDROXYISOBUT_DH

Important sites: ACT_SITE 171-171 BINDING 95-95 BINDING 239-239

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIAVIGLGNMGQPIARNVLQAGYELTVYNRTKQKTEDLVTEGAQAADTPRLAAKSADIV
CEEEEEECCCCCCHHHHHHHHCCCEEEEECCHHHHHHHHHHCCCCCCCCCHHHCCCCCEE
ITMLADDDSVSTVTFGEDGLLEGLAENGIHISMSTISVEFSEKLAAAHAEKGQFFLAAPV
EEEEECCCCCEEEEECCCHHHHHHHHCCEEEEEEEEEEHHHHHHHHHHHCCCCEEEECCC
LGRPDAAAKAALRIITAGPAEAKQAAKPLLDSLSQQIFDVGEESKTANAAKISINFLLVS
CCCCCHHHHHHEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEHHHHHHHH
MLEALSESFLMMEKYGLEQKQFLEIASALFGSPVYQNYGTIMAEQKFEPAGFKMSLGLKD
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHHHCCCEEECCCCCCCCEEEEECCCC
TNLALAAAKRVSANLPLAELAKSHFESGIEKGFGDLDWAALIKCIK
CHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHCC
>Mature Secondary Structure
MKIAVIGLGNMGQPIARNVLQAGYELTVYNRTKQKTEDLVTEGAQAADTPRLAAKSADIV
CEEEEEECCCCCCHHHHHHHHCCCEEEEECCHHHHHHHHHHCCCCCCCCCHHHCCCCCEE
ITMLADDDSVSTVTFGEDGLLEGLAENGIHISMSTISVEFSEKLAAAHAEKGQFFLAAPV
EEEEECCCCCEEEEECCCHHHHHHHHCCEEEEEEEEEEHHHHHHHHHHHCCCCEEEECCC
LGRPDAAAKAALRIITAGPAEAKQAAKPLLDSLSQQIFDVGEESKTANAAKISINFLLVS
CCCCCHHHHHHEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEHHHHHHHH
MLEALSESFLMMEKYGLEQKQFLEIASALFGSPVYQNYGTIMAEQKFEPAGFKMSLGLKD
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHHHCCCEEECCCCCCCCEEEEECCCC
TNLALAAAKRVSANLPLAELAKSHFESGIEKGFGDLDWAALIKCIK
CHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 3-hydroxy-2-methylpropanoate; NAD+

Specific reaction: 3-hydroxy-2-methylpropanoate + NAD+ = 2-methyl-3-oxopropanoate + NADH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969503; 9384377