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The map label for this gene is yfkC

Identifier: 16077861

GI number: 16077861

Start: 867164

End: 868006

Strand: Reverse

Name: yfkC

Synonym: BSU07940

Alternate gene names: 16077861

Gene position: 868006-867164 (Counterclockwise)

Preceding gene: 255767189

Following gene: 16077858

Centisome position: 20.59

GC content: 45.2

Gene sequence:

>843_bases
ATGCGGATGAAAGAAACACTTACGGAGATTTTTCAAAATAAAATCGTCGATATTCTCCTCGTTGCCGTTATCCTTTGGAT
CGGTGTCTTTATCATCAACCGGCTGGTACAGCTATTTTTTAAACGGACGGACTTTATTGAAGAAAAAAAGGAAAAAACGA
TTGAAAGCCTTGTCCGTTCTGTCACCCAGTATACGGCAACAATTGGTTTTATCTTTTACGTTATTTCTTTATTTGTGCAT
GATTTCGGCAAGATATTGGCCGGGGCGGGCGTAGCAGGTATCGTGATCGGTTTTGGCGCCCAATCGCTGATCAAAGATGT
GCTGGCTGGTGTATTCTTAATATATGAACGCCAGCTGCACAAAGGCGATTATGTCACCGTCAACAACTTGTTCAACGGGA
CAGTGGAGGAAATCGGCCTCCGCTCGCTGCAAATCCGGGAATGGAGCGGGAAGCTGCTCACCATCAGCAACGGCGAGGTC
AGACAAATAGAGAACTATAATATTGATTTTATGCGGATTACCGAATCCTTTCTTATCAGCTTTAAAGAAGATCCGGACCG
CGTGTACAGCGTGTTGGAAGAGGCATGTGACATGCTGAATGAAGAGCTTCGTGATTCATTGAAGCGGGATGAATTCGGCA
ATCCGACAGAACCGTTTCAAATCCACGGCATCACCGCATTGAATAAAATCAATCGCGGCGTCGAATTTACGGTTAAAGGC
ATGGTGAAAGATGATGATTATTTCAGTGCCAGCCTTGCAGTAAGACGGGTTCTCGTTCGCCAGCTCTACCAAAACAATGT
GCAAATGCTTGAGGAAGCGGTCCGAATCGAGAGAACCCAATAA

Upstream 100 bases:

>100_bases
TTGCCACTGCCCGAATGTCCGGTGCCTCGGACCGAATGTTCTTGTCAAAAACAGTTATTATCAGGATACAGACTTCACAA
AACGTCAAGCCAGGAGTTAG

Downstream 100 bases:

>100_bases
ACAAAAAAACTGATTCCGAGTTGGAATCAGTTTTTTATTTATCTTGGCAGTGAGGAAAGCTGAAAGCCAAACGTGACATG
ATCTTGAACCGGAATCCACG

Product: mechanosensitive ion channel

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 280; Mature: 280

Protein sequence:

>280_residues
MRMKETLTEIFQNKIVDILLVAVILWIGVFIINRLVQLFFKRTDFIEEKKEKTIESLVRSVTQYTATIGFIFYVISLFVH
DFGKILAGAGVAGIVIGFGAQSLIKDVLAGVFLIYERQLHKGDYVTVNNLFNGTVEEIGLRSLQIREWSGKLLTISNGEV
RQIENYNIDFMRITESFLISFKEDPDRVYSVLEEACDMLNEELRDSLKRDEFGNPTEPFQIHGITALNKINRGVEFTVKG
MVKDDDYFSASLAVRRVLVRQLYQNNVQMLEEAVRIERTQ

Sequences:

>Translated_280_residues
MRMKETLTEIFQNKIVDILLVAVILWIGVFIINRLVQLFFKRTDFIEEKKEKTIESLVRSVTQYTATIGFIFYVISLFVH
DFGKILAGAGVAGIVIGFGAQSLIKDVLAGVFLIYERQLHKGDYVTVNNLFNGTVEEIGLRSLQIREWSGKLLTISNGEV
RQIENYNIDFMRITESFLISFKEDPDRVYSVLEEACDMLNEELRDSLKRDEFGNPTEPFQIHGITALNKINRGVEFTVKG
MVKDDDYFSASLAVRRVLVRQLYQNNVQMLEEAVRIERTQ
>Mature_280_residues
MRMKETLTEIFQNKIVDILLVAVILWIGVFIINRLVQLFFKRTDFIEEKKEKTIESLVRSVTQYTATIGFIFYVISLFVH
DFGKILAGAGVAGIVIGFGAQSLIKDVLAGVFLIYERQLHKGDYVTVNNLFNGTVEEIGLRSLQIREWSGKLLTISNGEV
RQIENYNIDFMRITESFLISFKEDPDRVYSVLEEACDMLNEELRDSLKRDEFGNPTEPFQIHGITALNKINRGVEFTVKG
MVKDDDYFSASLAVRRVLVRQLYQNNVQMLEEAVRIERTQ

Specific function: May play a role in resistance to osmotic downshock

COG id: COG0668

COG function: function code M; Small-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscS (TC 1.A.23) family

Homologues:

Organism=Escherichia coli, GI87081787, Length=202, Percent_Identity=30.6930693069307, Blast_Score=66, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): YFKC_BACSU (O35043)

Other databases:

- EMBL:   D83967
- EMBL:   AL009126
- PIR:   G69807
- RefSeq:   NP_388675.1
- ProteinModelPortal:   O35043
- EnsemblBacteria:   EBBACT00000003290
- GeneID:   936137
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU07940
- NMPDR:   fig|224308.1.peg.794
- GenoList:   BSU07940
- GeneTree:   EBGT00050000001807
- HOGENOM:   HBG339648
- OMA:   MPAANMV
- ProtClustDB:   CLSK886875
- BioCyc:   BSUB:BSU07940-MONOMER
- InterPro:   IPR010920
- InterPro:   IPR006685
- InterPro:   IPR011014

Pfam domain/function: PF00924 MS_channel; SSF82861 MscS_transmembr; SSF50182 Sm_like_riboprot

EC number: NA

Molecular weight: Translated: 32207; Mature: 32207

Theoretical pI: Translated: 5.26; Mature: 5.26

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0xd781fdc)-; HASH(0xd222748)-; HASH(0xcc53c64)-;

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRMKETLTEIFQNKIVDILLVAVILWIGVFIINRLVQLFFKRTDFIEEKKEKTIESLVRS
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VTQYTATIGFIFYVISLFVHDFGKILAGAGVAGIVIGFGAQSLIKDVLAGVFLIYERQLH
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHC
KGDYVTVNNLFNGTVEEIGLRSLQIREWSGKLLTISNGEVRQIENYNIDFMRITESFLIS
CCCEEEECCHHCCCHHHHCCHHEEEEECCCCEEEECCCCEEECCCCCCHHHHHHHHHHHH
FKEDPDRVYSVLEEACDMLNEELRDSLKRDEFGNPTEPFQIHGITALNKINRGVEFTVKG
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECHHHHHHHCCCCEEEEEE
MVKDDDYFSASLAVRRVLVRQLYQNNVQMLEEAVRIERTQ
EECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCH
>Mature Secondary Structure
MRMKETLTEIFQNKIVDILLVAVILWIGVFIINRLVQLFFKRTDFIEEKKEKTIESLVRS
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VTQYTATIGFIFYVISLFVHDFGKILAGAGVAGIVIGFGAQSLIKDVLAGVFLIYERQLH
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHC
KGDYVTVNNLFNGTVEEIGLRSLQIREWSGKLLTISNGEVRQIENYNIDFMRITESFLIS
CCCEEEECCHHCCCHHHHCCHHEEEEECCCCEEEECCCCEEECCCCCCHHHHHHHHHHHH
FKEDPDRVYSVLEEACDMLNEELRDSLKRDEFGNPTEPFQIHGITALNKINRGVEFTVKG
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECHHHHHHHCCCCEEEEEE
MVKDDDYFSASLAVRRVLVRQLYQNNVQMLEEAVRIERTQ
EECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8969503; 9384377