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The map label for this gene is nasB

Identifier: 255767098

GI number: 255767098

Start: 360442

End: 362757

Strand: Reverse

Name: nasB

Synonym: BSU03320

Alternate gene names: 255767098

Gene position: 362757-360442 (Counterclockwise)

Preceding gene: 16077406

Following gene: 255767097

Centisome position: 8.61

GC content: 49.44

Gene sequence:

>2316_bases
ATGAAGAAACAAAGATTAGTGTTAGCGGGAAACGGCATGGCCGGAATCCGCTGTATTGAAGAAGTATTAAAGCTGAATCG
CCACATGTTTGAGATTGTCATTTTCGGAAGCGAGCCGCATCCCAATTACAATCGCATTCTCTTATCCTCTGTATTGCAGG
GTGAAGCGTCACTCGATGATATTACACTGAACAGCAAGGATTGGTACGACAAGCACGGGATCACGCTTTACACAGGCGAA
ACGGTTATTCAGATTGATACAGATCAGCAGCAGGTCATCACAGATCGGAAACGCACTCTGTCGTATGACAAATTAATAGT
GGCAACAGGCTCCTCCCCTCATATTCTCCCTATCCCCGGGGCAGACAAAAAAGGTGTCTATGGATTTCGGACAATAGAAG
ACTGCCAAGCGCTGATGAACATGGCCCAGCACTTTCAAAAGGCTGCGGTAATCGGAGCCGGCTTATTAGGACTGGAAGCT
GCAGTAGGTTTACAGCATCTCGGTATGGATGTCAGCGTCATTCACCACTCCGCCGGCATTATGCAAAAACAGCTCGATCA
AACAGCGGCGCGGCTGCTGCAAACAGAGCTGGAACAAAAAGGCCTAACCTTTCTTTTGGAGAAAGACACCGTCTCCATTT
CCGGCGCCACGAAGGCAGACCGAATCCATTTTAAAGATGGCTCTTCATTAAAAGCAGACTTGATTGTGATGGCGGCCGGC
GTCAAACCGAATATTGAATTGGCTGTATCCGCTGGAATCAAAGTGAACCGCGGGATCATCGTGAATGACTTCATGCAGAC
AAGCGAACCGAATATCTACGCCGTTGGTGAATGCGCTGAGCATAACGGAACGGTGTACGGATTGGTGGCTCCGCTTTATG
AGCAGGGAAAAGCGCTCGCCAGTCATATTTGCGGCGTTCCATGTGAGGAATATCAAGGCTCAGCACCATCTGCGGCGTTA
AAAATAGCTGGAATCGATGTGTGGTCAGCAGGAAAGATCCAAGAGGATGAACGCACGACGAGCATCAAAATCTACGACGA
ACAAGCAGGCGTTTATAAAAAAGCGCTTTTTGTAGATGACAAACTGGCCGGCGTTATTTTATTCGGAGACACCCGTGACA
AGCAGCGGCTTCTCGACAGCCTGCTTAAACAACGAGACATCTCCATTGCCAAAAAACAGATCATCGAACCGGAGACAAGC
GGCCCTTTATTTGAATCCATGCCTTCCAGTGAAACGATTTGCCAATGCAACACAGTGACGAAAGGCGCGATTGAAGACGC
GGTGCACACGAATAGCTTAACAACTGTTGAGGAAGTCAAGCACTGCACCAAAGCCACTGGGTCCTGCGGAGGATGCAAAC
CGCTCGTTGAAGACCTTTTGAGATACATGACCAACAGCGAATATACGAAGCCCGCCAGCACACCTTCGTTTTGCAGCTGT
ACTGATTTTACAGAAGACGACATCATCGCTGAGCTTCAGCGCAGGCCATTCACTAATCCGGCAGAAGTGATGAACCAGCT
TGACTGGAAAACAAAAAACGGATGCAGTACTTGTGTTCCTGCGATTCAATACTATCTGGAAATGCTGTACCCTGGGTTTG
TCCAGCCTGAGCCAGCCACTGAAGAGACATGCATCCTGATTCCGCAAATGTACGGCGGGCGGACAAACGCGGAGCAGCTA
AGAACCATCGCCAACATCATTGAGGCATACAGCATTCCCGACGTATCCATCACTCACGGCCAAAGGCTGAAGCTTTCCGG
GATCAAGCCTGCGGATCTCCCGAACATGAAAAAAGACCTGAAAATGCCCGTTTACACTAATGAACACCGACACGCGCTGC
AAAGCATCAAAGCATGTACATGCGGGCAAAATCGATCGATTCAGCAGCTGGCAGCCCAAATCGAAAGACAACTTGAGATG
CTTCCCTTACCCGCTCCCATTTCTATCAGCTTATCATGTGAAACAGACTGCACCGAGGCCGCCCTGCAAGATGTCGGCGC
AATTCGGACACAAGCTGGCTGGGACATTCACATCGGCGGCGTCCGGGGAACTCATGCCCGTTCAGGAGCTTTGTTTTGCG
TGACTGAGAACGAAGACAGCACTGCCGGCATGATCAAAGGACTGATCCAGTATTATCGGGAAACGGCTCATTACTTAGAA
GGTGTTCATCAGTGGATAGACCGTCTCGGGATCGTTCATATCAGAGAAGTGCTATTCGAAGAAGACCTAAGGGCACAGCT
GCTGGAAAGCCTCCAAACAGATCTGTCACTGATTCAAAACCCGACTGTAGAGACGGGCGCATATAAGAAAGGATGA

Upstream 100 bases:

>100_bases
TGTGTAAGTTTTTGTGACACGTTTAATGCGTTAACAATGCATTGTGACATAATTTTTAATAGGAGAAAACTTACGAGCTT
CGCCAAAGGAGGTCTGATAC

Downstream 100 bases:

>100_bases
GGCATTTTGACTGAACGACTGCTTAGATATTTCCGTGATAAACAGCAAGACGTCCAATCAGAAAAAACATATGACACTCA
ATGCCCGTTTTGCAGCATGC

Product: assimilatory nitrate reductase electron transfer subunit

Products: nitrite; NADH; NADPH; H+

Alternate protein names: NA

Number of amino acids: Translated: 771; Mature: 771

Protein sequence:

>771_residues
MKKQRLVLAGNGMAGIRCIEEVLKLNRHMFEIVIFGSEPHPNYNRILLSSVLQGEASLDDITLNSKDWYDKHGITLYTGE
TVIQIDTDQQQVITDRKRTLSYDKLIVATGSSPHILPIPGADKKGVYGFRTIEDCQALMNMAQHFQKAAVIGAGLLGLEA
AVGLQHLGMDVSVIHHSAGIMQKQLDQTAARLLQTELEQKGLTFLLEKDTVSISGATKADRIHFKDGSSLKADLIVMAAG
VKPNIELAVSAGIKVNRGIIVNDFMQTSEPNIYAVGECAEHNGTVYGLVAPLYEQGKALASHICGVPCEEYQGSAPSAAL
KIAGIDVWSAGKIQEDERTTSIKIYDEQAGVYKKALFVDDKLAGVILFGDTRDKQRLLDSLLKQRDISIAKKQIIEPETS
GPLFESMPSSETICQCNTVTKGAIEDAVHTNSLTTVEEVKHCTKATGSCGGCKPLVEDLLRYMTNSEYTKPASTPSFCSC
TDFTEDDIIAELQRRPFTNPAEVMNQLDWKTKNGCSTCVPAIQYYLEMLYPGFVQPEPATEETCILIPQMYGGRTNAEQL
RTIANIIEAYSIPDVSITHGQRLKLSGIKPADLPNMKKDLKMPVYTNEHRHALQSIKACTCGQNRSIQQLAAQIERQLEM
LPLPAPISISLSCETDCTEAALQDVGAIRTQAGWDIHIGGVRGTHARSGALFCVTENEDSTAGMIKGLIQYYRETAHYLE
GVHQWIDRLGIVHIREVLFEEDLRAQLLESLQTDLSLIQNPTVETGAYKKG

Sequences:

>Translated_771_residues
MKKQRLVLAGNGMAGIRCIEEVLKLNRHMFEIVIFGSEPHPNYNRILLSSVLQGEASLDDITLNSKDWYDKHGITLYTGE
TVIQIDTDQQQVITDRKRTLSYDKLIVATGSSPHILPIPGADKKGVYGFRTIEDCQALMNMAQHFQKAAVIGAGLLGLEA
AVGLQHLGMDVSVIHHSAGIMQKQLDQTAARLLQTELEQKGLTFLLEKDTVSISGATKADRIHFKDGSSLKADLIVMAAG
VKPNIELAVSAGIKVNRGIIVNDFMQTSEPNIYAVGECAEHNGTVYGLVAPLYEQGKALASHICGVPCEEYQGSAPSAAL
KIAGIDVWSAGKIQEDERTTSIKIYDEQAGVYKKALFVDDKLAGVILFGDTRDKQRLLDSLLKQRDISIAKKQIIEPETS
GPLFESMPSSETICQCNTVTKGAIEDAVHTNSLTTVEEVKHCTKATGSCGGCKPLVEDLLRYMTNSEYTKPASTPSFCSC
TDFTEDDIIAELQRRPFTNPAEVMNQLDWKTKNGCSTCVPAIQYYLEMLYPGFVQPEPATEETCILIPQMYGGRTNAEQL
RTIANIIEAYSIPDVSITHGQRLKLSGIKPADLPNMKKDLKMPVYTNEHRHALQSIKACTCGQNRSIQQLAAQIERQLEM
LPLPAPISISLSCETDCTEAALQDVGAIRTQAGWDIHIGGVRGTHARSGALFCVTENEDSTAGMIKGLIQYYRETAHYLE
GVHQWIDRLGIVHIREVLFEEDLRAQLLESLQTDLSLIQNPTVETGAYKKG
>Mature_771_residues
MKKQRLVLAGNGMAGIRCIEEVLKLNRHMFEIVIFGSEPHPNYNRILLSSVLQGEASLDDITLNSKDWYDKHGITLYTGE
TVIQIDTDQQQVITDRKRTLSYDKLIVATGSSPHILPIPGADKKGVYGFRTIEDCQALMNMAQHFQKAAVIGAGLLGLEA
AVGLQHLGMDVSVIHHSAGIMQKQLDQTAARLLQTELEQKGLTFLLEKDTVSISGATKADRIHFKDGSSLKADLIVMAAG
VKPNIELAVSAGIKVNRGIIVNDFMQTSEPNIYAVGECAEHNGTVYGLVAPLYEQGKALASHICGVPCEEYQGSAPSAAL
KIAGIDVWSAGKIQEDERTTSIKIYDEQAGVYKKALFVDDKLAGVILFGDTRDKQRLLDSLLKQRDISIAKKQIIEPETS
GPLFESMPSSETICQCNTVTKGAIEDAVHTNSLTTVEEVKHCTKATGSCGGCKPLVEDLLRYMTNSEYTKPASTPSFCSC
TDFTEDDIIAELQRRPFTNPAEVMNQLDWKTKNGCSTCVPAIQYYLEMLYPGFVQPEPATEETCILIPQMYGGRTNAEQL
RTIANIIEAYSIPDVSITHGQRLKLSGIKPADLPNMKKDLKMPVYTNEHRHALQSIKACTCGQNRSIQQLAAQIERQLEM
LPLPAPISISLSCETDCTEAALQDVGAIRTQAGWDIHIGGVRGTHARSGALFCVTENEDSTAGMIKGLIQYYRETAHYLE
GVHQWIDRLGIVHIREVLFEEDLRAQLLESLQTDLSLIQNPTVETGAYKKG

Specific function: Required for nitrate assimilation

COG id: COG1251

COG function: function code C; NAD(P)H-nitrite reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI21389617, Length=276, Percent_Identity=28.2608695652174, Blast_Score=111, Evalue=2e-24,
Organism=Homo sapiens, GI226437568, Length=276, Percent_Identity=28.2608695652174, Blast_Score=111, Evalue=2e-24,
Organism=Homo sapiens, GI65787454, Length=276, Percent_Identity=28.2608695652174, Blast_Score=111, Evalue=2e-24,
Organism=Homo sapiens, GI4757732, Length=218, Percent_Identity=28.4403669724771, Blast_Score=87, Evalue=6e-17,
Organism=Homo sapiens, GI22202629, Length=218, Percent_Identity=28.4403669724771, Blast_Score=87, Evalue=8e-17,
Organism=Escherichia coli, GI1789765, Length=786, Percent_Identity=31.4249363867684, Blast_Score=323, Evalue=2e-89,
Organism=Escherichia coli, GI1789065, Length=326, Percent_Identity=24.8466257668712, Blast_Score=125, Evalue=9e-30,
Organism=Escherichia coli, GI1788892, Length=279, Percent_Identity=25.089605734767, Blast_Score=102, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI17559934, Length=278, Percent_Identity=28.4172661870504, Blast_Score=106, Evalue=6e-23,
Organism=Caenorhabditis elegans, GI32565766, Length=211, Percent_Identity=27.0142180094787, Blast_Score=66, Evalue=6e-11,
Organism=Drosophila melanogaster, GI281359715, Length=288, Percent_Identity=24.3055555555556, Blast_Score=100, Evalue=3e-21,
Organism=Drosophila melanogaster, GI281359713, Length=288, Percent_Identity=24.3055555555556, Blast_Score=100, Evalue=3e-21,
Organism=Drosophila melanogaster, GI24639250, Length=288, Percent_Identity=24.3055555555556, Blast_Score=100, Evalue=3e-21,
Organism=Drosophila melanogaster, GI18543267, Length=288, Percent_Identity=24.3055555555556, Blast_Score=100, Evalue=3e-21,
Organism=Drosophila melanogaster, GI24639252, Length=288, Percent_Identity=24.3055555555556, Blast_Score=100, Evalue=3e-21,
Organism=Drosophila melanogaster, GI24639257, Length=288, Percent_Identity=24.3055555555556, Blast_Score=100, Evalue=4e-21,
Organism=Drosophila melanogaster, GI24585130, Length=282, Percent_Identity=23.0496453900709, Blast_Score=97, Evalue=4e-20,
Organism=Drosophila melanogaster, GI28573993, Length=265, Percent_Identity=25.6603773584906, Blast_Score=81, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24581020, Length=265, Percent_Identity=25.6603773584906, Blast_Score=81, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NASB_BACSU (P42433)

Other databases:

- EMBL:   D30689
- EMBL:   D50453
- EMBL:   AL009126
- PIR:   I40027
- RefSeq:   NP_388214.2
- ProteinModelPortal:   P42433
- SMR:   P42433
- EnsemblBacteria:   EBBACT00000001901
- GeneID:   938328
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU03320
- NMPDR:   fig|224308.1.peg.333
- GenoList:   BSU03320
- GeneTree:   EBGT00070000031876
- HOGENOM:   HBG285339
- ProtClustDB:   CLSK886669
- BioCyc:   BSUB:BSU03320-MONOMER
- GO:   GO:0006810
- InterPro:   IPR007419
- InterPro:   IPR013027
- InterPro:   IPR005117
- InterPro:   IPR012744
- InterPro:   IPR017121
- InterPro:   IPR006067
- InterPro:   IPR001327
- PIRSF:   PIRSF037149
- PRINTS:   PR00368
- TIGRFAMs:   TIGR02374

Pfam domain/function: PF04324 Fer2_BFD; PF01077 NIR_SIR; PF03460 NIR_SIR_ferr; PF00070 Pyr_redox; PF07992 Pyr_redox_2; SSF55124 NiR_SiRalpha_1/3

EC number: 1.7.1.4

Molecular weight: Translated: 84683; Mature: 84683

Theoretical pI: Translated: 5.71; Mature: 5.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKQRLVLAGNGMAGIRCIEEVLKLNRHMFEIVIFGSEPHPNYNRILLSSVLQGEASLDD
CCCCEEEEECCCCHHHHHHHHHHHHCCCEEEEEEECCCCCCCHHHHHHHHHHCCCCCCCC
ITLNSKDWYDKHGITLYTGETVIQIDTDQQQVITDRKRTLSYDKLIVATGSSPHILPIPG
EEECCCCCCCCCCEEEEECCEEEEECCCHHHHHHHHHHHCCCCEEEEEECCCCCEEECCC
ADKKGVYGFRTIEDCQALMNMAQHFQKAAVIGAGLLGLEAAVGLQHLGMDVSVIHHSAGI
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHH
MQKQLDQTAARLLQTELEQKGLTFLLEKDTVSISGATKADRIHFKDGSSLKADLIVMAAG
HHHHHHHHHHHHHHHHHHHCCCEEEEECCEEEECCCCCCCEEEECCCCCCCEEEEEEEEC
VKPNIELAVSAGIKVNRGIIVNDFMQTSEPNIYAVGECAEHNGTVYGLVAPLYEQGKALA
CCCCEEEEEECCEEECCCEEEECHHHCCCCCEEEEEEHHCCCCEEEEEEHHHHHHHHHHH
SHICGVPCEEYQGSAPSAALKIAGIDVWSAGKIQEDERTTSIKIYDEQAGVYKKALFVDD
HHHCCCCHHHHCCCCCCCEEEEEEEEEECCCCCCCCCCCEEEEEEECCCCHHHHHEEECC
KLAGVILFGDTRDKQRLLDSLLKQRDISIAKKQIIEPETSGPLFESMPSSETICQCNTVT
CCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCCCCCCEEEECCCC
KGAIEDAVHTNSLTTVEEVKHCTKATGSCGGCKPLVEDLLRYMTNSEYTKPASTPSFCSC
CHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCC
TDFTEDDIIAELQRRPFTNPAEVMNQLDWKTKNGCSTCVPAIQYYLEMLYPGFVQPEPAT
CCCCHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCC
EETCILIPQMYGGRTNAEQLRTIANIIEAYSIPDVSITHGQRLKLSGIKPADLPNMKKDL
CCEEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCEEECCCCEEEECCCCCCCCCCCHHHC
KMPVYTNEHRHALQSIKACTCGQNRSIQQLAAQIERQLEMLPLPAPISISLSCETDCTEA
CCCCEECHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEECCCCHHHH
ALQDVGAIRTQAGWDIHIGGVRGTHARSGALFCVTENEDSTAGMIKGLIQYYRETAHYLE
HHHHHHHHHCCCCCEEEECCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHH
GVHQWIDRLGIVHIREVLFEEDLRAQLLESLQTDLSLIQNPTVETGAYKKG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure
MKKQRLVLAGNGMAGIRCIEEVLKLNRHMFEIVIFGSEPHPNYNRILLSSVLQGEASLDD
CCCCEEEEECCCCHHHHHHHHHHHHCCCEEEEEEECCCCCCCHHHHHHHHHHCCCCCCCC
ITLNSKDWYDKHGITLYTGETVIQIDTDQQQVITDRKRTLSYDKLIVATGSSPHILPIPG
EEECCCCCCCCCCEEEEECCEEEEECCCHHHHHHHHHHHCCCCEEEEEECCCCCEEECCC
ADKKGVYGFRTIEDCQALMNMAQHFQKAAVIGAGLLGLEAAVGLQHLGMDVSVIHHSAGI
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHH
MQKQLDQTAARLLQTELEQKGLTFLLEKDTVSISGATKADRIHFKDGSSLKADLIVMAAG
HHHHHHHHHHHHHHHHHHHCCCEEEEECCEEEECCCCCCCEEEECCCCCCCEEEEEEEEC
VKPNIELAVSAGIKVNRGIIVNDFMQTSEPNIYAVGECAEHNGTVYGLVAPLYEQGKALA
CCCCEEEEEECCEEECCCEEEECHHHCCCCCEEEEEEHHCCCCEEEEEEHHHHHHHHHHH
SHICGVPCEEYQGSAPSAALKIAGIDVWSAGKIQEDERTTSIKIYDEQAGVYKKALFVDD
HHHCCCCHHHHCCCCCCCEEEEEEEEEECCCCCCCCCCCEEEEEEECCCCHHHHHEEECC
KLAGVILFGDTRDKQRLLDSLLKQRDISIAKKQIIEPETSGPLFESMPSSETICQCNTVT
CCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCCCCCCEEEECCCC
KGAIEDAVHTNSLTTVEEVKHCTKATGSCGGCKPLVEDLLRYMTNSEYTKPASTPSFCSC
CHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCC
TDFTEDDIIAELQRRPFTNPAEVMNQLDWKTKNGCSTCVPAIQYYLEMLYPGFVQPEPAT
CCCCHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCC
EETCILIPQMYGGRTNAEQLRTIANIIEAYSIPDVSITHGQRLKLSGIKPADLPNMKKDL
CCEEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCEEECCCCEEEECCCCCCCCCCCHHHC
KMPVYTNEHRHALQSIKACTCGQNRSIQQLAAQIERQLEMLPLPAPISISLSCETDCTEA
CCCCEECHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEECCCCHHHH
ALQDVGAIRTQAGWDIHIGGVRGTHARSGALFCVTENEDSTAGMIKGLIQYYRETAHYLE
HHHHHHHHHCCCCCEEEECCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHH
GVHQWIDRLGIVHIREVLFEEDLRAQLLESLQTDLSLIQNPTVETGAYKKG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD; NAD+; NADH; Siroheme [C]

Metal ions: Iron [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.4 {NO2-}} 0.005 {NO2-}} 0.016 {NADH}} 5.3 {hydroxylamine}} [C]

Substrates: ammonium hydroxide; NAD+; NADP+; H2O

Specific reaction: ammonium hydroxide + 3 NAD(P)+ + H2O = nitrite + 3 NAD(P)H + 3 H+

General reaction: Redox reaction [C]

Inhibitor: 1, 10-Phenanthroline; 2, 2'-Bipyridine; Arsenite; CN-; NAD+; NADP+; NaN3; p-Chloromercuribenzoate; SO32- [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7868621; 8969502; 9384377