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The map label for this gene is nasC

Identifier: 255767097

GI number: 255767097

Start: 358303

End: 360435

Strand: Reverse

Name: nasC

Synonym: BSU03310

Alternate gene names: 255767097

Gene position: 360435-358303 (Counterclockwise)

Preceding gene: 255767098

Following gene: 16077399

Centisome position: 8.55

GC content: 48.43

Gene sequence:

>2133_bases
TTGACTGAACGACTGCTTAGATATTTCCGTGATAAACAGCAAGACGTCCAATCAGAAAAAACATATGACACTCAATGCCC
GTTTTGCAGCATGCAGTGCAAAATGCAGCTCGTGGAACAAACCATCGTCACGCGCAAAAAGTACACTGCGATCGGGATTG
ATAATCCTACGACACAGGGTCGGCTATGCATCAAGGGCATGAATGCTCACCAGCATGCCTTGAACTCCTCCCGCATCACC
CGGCCGCTGCTGAAGAAAAACGGCGAGTTTATGCCTGTTTCCTGGGAAGAAGCACTGAATCATATCAAAGACCAAGTGAC
AATGATCCAAACTGAACACGGACATGACGCCATGGCGGTATACGGAAGCGCCTCGATTACAAATGAAGAGGCGTATTTGT
TAGGGAAGTTCGCACGAGTAGGCTTACAGACAAAATACATCGACTACAACGGGAGACTTTGTATGTCCGCAGCAGCCACC
GCAGCCAACCAAACATTCGGCGCGGACAGAGGCTTAACGAATCCTTTATCAGACATCCCCCACACCCGTGTGATCATTCT
GGCCGGCACCAATATTGCGGAATGCCAGCCTACGATTATGCCGTATTTTGAAAAAGCGAAAGAAAACGGAGCTTACTTCA
TTGCCATTGATCCTCGTGAAACAGCGACAACGAAAATCGCCGATCTTCATCTGAAAATCAAACCCGGCACAGACGCCGCC
CTTGCCAACGGTCTCGTCAAAATCATCATTGACGAACAGCTTATAAACGAGGATTTCATTCAATCACGAACAAATGGATT
CGAAGAGCTGAAGCAGCATACTGACTCGTTGGACCTGAATGACATCGCCGAACAGACGAGTGTCTCGCTGGTGGATATAA
GAAAAGCTGCTGTGAAGTTTGCCAAGGAAACGTCCGGCATGCTGTTTACAGCCCGCGGGATTGAACAGCAAACCGATGGA
ACAGCGGCTGTGAAAGGCTTCTTAAACATGGTGCTGATCACAGGAAAAATCGGCAAACCTTACTCGGGCTACGGGGCGAT
CACCGGACAAGGCAACGGGCAAGGCGCCAGAGAGCATGGCCAAAAAGCCGATCAGCTTCCAGGCTACCGTTCTATCGAAA
ACGAAGAACACCGGGCGCATATTGCGAAAGTGTGGGGAATCCATCAGGATGAACTCCCGCGAAAAGGTGTCTCAGCCTAT
GAAATGATGGAAAAAATCAATGACGGCGACATCAAAGGACTGTTCCTCATGTGCTCCAACCCTGCTGTTTCCAGTCCAAA
TGCAAATCTCGTGAAAAAGGCTTTAAGAAGACTGACATTCTTTGTCGCAATCGATTTGTTTATTTCTGAGACTGCGAAAT
ACGCAGATGTGATTTTACCTGCCTCCTCTTACTTAGAAGATGAAGGCACCATGACAAATGTAGAAGGACGCGTGACATTA
AGAGAAGCAAGCCGGCCATGCCCCGGCGAAGCAAAGCATGATTGGCAGATCATTTGTGATCTCGCATCCGCTCTCGGCAA
GGGCCGCTACTTTTCTTATACGTCAGCGGAGGATATTTTCAATGAATTGAGAGAAGCGAGCCGAGGCGGGATTGCCGATT
ATTCAGGCATCTCATACGGCAGGCTCAGACGTGAAGGCGGCATTCATTGGCCGTGTCCGGAATCTGATCATCCCGGAACT
GGGCGCTTGTTTACAGAATCATTTGCTCATCCGGATCAAAAAGCGGCATTAAGTGTGATTCCAAACGAACCACCTGTTCC
AAAAGAGAAGCCGACAGCCGATTATCCGCTTTATTTAACGACAGGCAGAGTCATGTCTCACTACTTAACAGGCGTCCAAA
CAAGAAAAAGCGCCGCCCTTGCCGCGAGACATTTTGAATCGTTTATGGAGATCCATCCGCAAACAGCAGCGACCTACAAT
ATCGAGGACCGTGTACTGGTAAAAATAGAATCGCCGCGGGGAAGCATCACCGTCCGCAGCAAATTATCAGAACAAATCAG
AAAAGATACCGTTTTTGTTCCCATCCATTGGGCAGACGCTCAAAATGTGAATGATTTAATCGGGGAAGCCTTAGATCCAG
CCTGTAAAATGCCCGGTTTTAAGGTATGCGCCGTCCGAATCATACCTATTTAA

Upstream 100 bases:

>100_bases
AGACCTAAGGGCACAGCTGCTGGAAAGCCTCCAAACAGATCTGTCACTGATTCAAAACCCGACTGTAGAGACGGGCGCAT
ATAAGAAAGGATGAGGCATT

Downstream 100 bases:

>100_bases
ATTTTTCATAAAATTTTTAGAACTTTTCGTATATTTTGTTACATTTTATAACATGTATCTTAAATATTCCTTTTTGCTGT
GCTACGCTGGAAAAAACATT

Product: assimilatory nitrate reductase catalytic subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 710; Mature: 709

Protein sequence:

>710_residues
MTERLLRYFRDKQQDVQSEKTYDTQCPFCSMQCKMQLVEQTIVTRKKYTAIGIDNPTTQGRLCIKGMNAHQHALNSSRIT
RPLLKKNGEFMPVSWEEALNHIKDQVTMIQTEHGHDAMAVYGSASITNEEAYLLGKFARVGLQTKYIDYNGRLCMSAAAT
AANQTFGADRGLTNPLSDIPHTRVIILAGTNIAECQPTIMPYFEKAKENGAYFIAIDPRETATTKIADLHLKIKPGTDAA
LANGLVKIIIDEQLINEDFIQSRTNGFEELKQHTDSLDLNDIAEQTSVSLVDIRKAAVKFAKETSGMLFTARGIEQQTDG
TAAVKGFLNMVLITGKIGKPYSGYGAITGQGNGQGAREHGQKADQLPGYRSIENEEHRAHIAKVWGIHQDELPRKGVSAY
EMMEKINDGDIKGLFLMCSNPAVSSPNANLVKKALRRLTFFVAIDLFISETAKYADVILPASSYLEDEGTMTNVEGRVTL
REASRPCPGEAKHDWQIICDLASALGKGRYFSYTSAEDIFNELREASRGGIADYSGISYGRLRREGGIHWPCPESDHPGT
GRLFTESFAHPDQKAALSVIPNEPPVPKEKPTADYPLYLTTGRVMSHYLTGVQTRKSAALAARHFESFMEIHPQTAATYN
IEDRVLVKIESPRGSITVRSKLSEQIRKDTVFVPIHWADAQNVNDLIGEALDPACKMPGFKVCAVRIIPI

Sequences:

>Translated_710_residues
MTERLLRYFRDKQQDVQSEKTYDTQCPFCSMQCKMQLVEQTIVTRKKYTAIGIDNPTTQGRLCIKGMNAHQHALNSSRIT
RPLLKKNGEFMPVSWEEALNHIKDQVTMIQTEHGHDAMAVYGSASITNEEAYLLGKFARVGLQTKYIDYNGRLCMSAAAT
AANQTFGADRGLTNPLSDIPHTRVIILAGTNIAECQPTIMPYFEKAKENGAYFIAIDPRETATTKIADLHLKIKPGTDAA
LANGLVKIIIDEQLINEDFIQSRTNGFEELKQHTDSLDLNDIAEQTSVSLVDIRKAAVKFAKETSGMLFTARGIEQQTDG
TAAVKGFLNMVLITGKIGKPYSGYGAITGQGNGQGAREHGQKADQLPGYRSIENEEHRAHIAKVWGIHQDELPRKGVSAY
EMMEKINDGDIKGLFLMCSNPAVSSPNANLVKKALRRLTFFVAIDLFISETAKYADVILPASSYLEDEGTMTNVEGRVTL
REASRPCPGEAKHDWQIICDLASALGKGRYFSYTSAEDIFNELREASRGGIADYSGISYGRLRREGGIHWPCPESDHPGT
GRLFTESFAHPDQKAALSVIPNEPPVPKEKPTADYPLYLTTGRVMSHYLTGVQTRKSAALAARHFESFMEIHPQTAATYN
IEDRVLVKIESPRGSITVRSKLSEQIRKDTVFVPIHWADAQNVNDLIGEALDPACKMPGFKVCAVRIIPI
>Mature_709_residues
TERLLRYFRDKQQDVQSEKTYDTQCPFCSMQCKMQLVEQTIVTRKKYTAIGIDNPTTQGRLCIKGMNAHQHALNSSRITR
PLLKKNGEFMPVSWEEALNHIKDQVTMIQTEHGHDAMAVYGSASITNEEAYLLGKFARVGLQTKYIDYNGRLCMSAAATA
ANQTFGADRGLTNPLSDIPHTRVIILAGTNIAECQPTIMPYFEKAKENGAYFIAIDPRETATTKIADLHLKIKPGTDAAL
ANGLVKIIIDEQLINEDFIQSRTNGFEELKQHTDSLDLNDIAEQTSVSLVDIRKAAVKFAKETSGMLFTARGIEQQTDGT
AAVKGFLNMVLITGKIGKPYSGYGAITGQGNGQGAREHGQKADQLPGYRSIENEEHRAHIAKVWGIHQDELPRKGVSAYE
MMEKINDGDIKGLFLMCSNPAVSSPNANLVKKALRRLTFFVAIDLFISETAKYADVILPASSYLEDEGTMTNVEGRVTLR
EASRPCPGEAKHDWQIICDLASALGKGRYFSYTSAEDIFNELREASRGGIADYSGISYGRLRREGGIHWPCPESDHPGTG
RLFTESFAHPDQKAALSVIPNEPPVPKEKPTADYPLYLTTGRVMSHYLTGVQTRKSAALAARHFESFMEIHPQTAATYNI
EDRVLVKIESPRGSITVRSKLSEQIRKDTVFVPIHWADAQNVNDLIGEALDPACKMPGFKVCAVRIIPI

Specific function: Nitrate reductase is a key enzyme involved in the first step of nitrate assimilation in plants, fungi and bacteria

COG id: COG0243

COG function: function code C; Anaerobic dehydrogenases, typically selenocysteine-containing

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic molybdopterin-containing oxidoreductase family

Homologues:

Organism=Escherichia coli, GI3868721, Length=700, Percent_Identity=32.2857142857143, Blast_Score=374, Evalue=1e-105,
Organism=Escherichia coli, GI1788534, Length=789, Percent_Identity=27.2496831432193, Blast_Score=240, Evalue=2e-64,
Organism=Escherichia coli, GI3868720, Length=623, Percent_Identity=23.1139646869984, Blast_Score=135, Evalue=1e-32,
Organism=Escherichia coli, GI3868719, Length=614, Percent_Identity=23.4527687296417, Blast_Score=132, Evalue=5e-32,
Organism=Escherichia coli, GI1787778, Length=520, Percent_Identity=24.2307692307692, Blast_Score=114, Evalue=2e-26,
Organism=Escherichia coli, GI171474008, Length=554, Percent_Identity=23.826714801444, Blast_Score=109, Evalue=5e-25,
Organism=Escherichia coli, GI1787870, Length=544, Percent_Identity=23.1617647058824, Blast_Score=102, Evalue=1e-22,
Organism=Escherichia coli, GI87081797, Length=743, Percent_Identity=23.2839838492598, Blast_Score=101, Evalue=2e-22,
Organism=Escherichia coli, GI87081994, Length=694, Percent_Identity=23.7752161383285, Blast_Score=94, Evalue=4e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NASC_BACSU (P42434)

Other databases:

- EMBL:   D50453
- EMBL:   AL009126
- EMBL:   D30689
- PIR:   E69665
- RefSeq:   NP_388213.2
- ProteinModelPortal:   P42434
- SMR:   P42434
- EnsemblBacteria:   EBBACT00000002620
- GeneID:   938321
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU03310
- NMPDR:   fig|224308.1.peg.332
- GenoList:   BSU03310
- GeneTree:   EBGT00070000031948
- HOGENOM:   HBG715033
- ProtClustDB:   CLSK872771
- BioCyc:   BSUB:BSU03310-MONOMER
- BRENDA:   1.7.99.4
- InterPro:   IPR009010
- InterPro:   IPR006657
- InterPro:   IPR006656
- InterPro:   IPR006963
- InterPro:   IPR006655
- Gene3D:   G3DSA:2.40.40.20
- SMART:   SM00926

Pfam domain/function: PF04879 Molybdop_Fe4S4; PF00384 Molybdopterin; PF01568 Molydop_binding; SSF50692 Asp_decarb_fold

EC number: =1.7.99.4

Molecular weight: Translated: 78622; Mature: 78491

Theoretical pI: Translated: 7.17; Mature: 7.17

Prosite motif: PS00551 MOLYBDOPTERIN_PROK_1; PS00490 MOLYBDOPTERIN_PROK_2; PS00932 MOLYBDOPTERIN_PROK_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTERLLRYFRDKQQDVQSEKTYDTQCPFCSMQCKMQLVEQTIVTRKKYTAIGIDNPTTQG
CHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCC
RLCIKGMNAHQHALNSSRITRPLLKKNGEFMPVSWEEALNHIKDQVTMIQTEHGHDAMAV
EEEEECCCHHHHHHCHHHHHHHHHHCCCCEEEECHHHHHHHHHHHEEEEEECCCCCEEEE
YGSASITNEEAYLLGKFARVGLQTKYIDYNGRLCMSAAATAANQTFGADRGLTNPLSDIP
ECCCCCCCCHHHHHHHHHHHCCEEEEEECCCCEEEHHHHHHHCCCCCCCCCCCCCHHHCC
HTRVIILAGTNIAECQPTIMPYFEKAKENGAYFIAIDPRETATTKIADLHLKIKPGTDAA
CCEEEEEECCCHHCCCCHHHHHHHHHHCCCCEEEEECCCCCCCEEEEEEEEEECCCCCHH
LANGLVKIIIDEQLINEDFIQSRTNGFEELKQHTDSLDLNDIAEQTSVSLVDIRKAAVKF
HHCCEEEEEECHHHHHHHHHHHHCCCHHHHHHHCCCCCHHHHHHHHCCHHHHHHHHHHHH
AKETSGMLFTARGIEQQTDGTAAVKGFLNMVLITGKIGKPYSGYGAITGQGNGQGAREHG
HHHCCCEEEEECCCCCCCCCHHHHHHHHHEEEEECCCCCCCCCCEEEECCCCCCCHHHHC
QKADQLPGYRSIENEEHRAHIAKVWGIHQDELPRKGVSAYEMMEKINDGDIKGLFLMCSN
CHHHHCCCCCCCCCHHHHHHHHHHHCCCHHHCCCCCCHHHHHHHHCCCCCCEEEEEEECC
PAVSSPNANLVKKALRRLTFFVAIDLFISETAKYADVILPASSYLEDEGTMTNVEGRVTL
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCHHHHCCCCCEECCCCEEEE
REASRPCPGEAKHDWQIICDLASALGKGRYFSYTSAEDIFNELREASRGGIADYSGISYG
ECCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHCCCCCCCCCCCHH
RLRREGGIHWPCPESDHPGTGRLFTESFAHPDQKAALSVIPNEPPVPKEKPTADYPLYLT
HHHHCCCCCCCCCCCCCCCCCCEEHHHHCCCCCCCEEEECCCCCCCCCCCCCCCCCEEEE
TGRVMSHYLTGVQTRKSAALAARHFESFMEIHPQTAATYNIEDRVLVKIESPRGSITVRS
HHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCEEEEEEECCCCCEEEHH
KLSEQIRKDTVFVPIHWADAQNVNDLIGEALDPACKMPGFKVCAVRIIPI
HHHHHHHHCEEEEEEEECCCCCHHHHHHHHCCHHHCCCCCEEEEEEEECC
>Mature Secondary Structure 
TERLLRYFRDKQQDVQSEKTYDTQCPFCSMQCKMQLVEQTIVTRKKYTAIGIDNPTTQG
HHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCC
RLCIKGMNAHQHALNSSRITRPLLKKNGEFMPVSWEEALNHIKDQVTMIQTEHGHDAMAV
EEEEECCCHHHHHHCHHHHHHHHHHCCCCEEEECHHHHHHHHHHHEEEEEECCCCCEEEE
YGSASITNEEAYLLGKFARVGLQTKYIDYNGRLCMSAAATAANQTFGADRGLTNPLSDIP
ECCCCCCCCHHHHHHHHHHHCCEEEEEECCCCEEEHHHHHHHCCCCCCCCCCCCCHHHCC
HTRVIILAGTNIAECQPTIMPYFEKAKENGAYFIAIDPRETATTKIADLHLKIKPGTDAA
CCEEEEEECCCHHCCCCHHHHHHHHHHCCCCEEEEECCCCCCCEEEEEEEEEECCCCCHH
LANGLVKIIIDEQLINEDFIQSRTNGFEELKQHTDSLDLNDIAEQTSVSLVDIRKAAVKF
HHCCEEEEEECHHHHHHHHHHHHCCCHHHHHHHCCCCCHHHHHHHHCCHHHHHHHHHHHH
AKETSGMLFTARGIEQQTDGTAAVKGFLNMVLITGKIGKPYSGYGAITGQGNGQGAREHG
HHHCCCEEEEECCCCCCCCCHHHHHHHHHEEEEECCCCCCCCCCEEEECCCCCCCHHHHC
QKADQLPGYRSIENEEHRAHIAKVWGIHQDELPRKGVSAYEMMEKINDGDIKGLFLMCSN
CHHHHCCCCCCCCCHHHHHHHHHHHCCCHHHCCCCCCHHHHHHHHCCCCCCEEEEEEECC
PAVSSPNANLVKKALRRLTFFVAIDLFISETAKYADVILPASSYLEDEGTMTNVEGRVTL
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCHHHHCCCCCEECCCCEEEE
REASRPCPGEAKHDWQIICDLASALGKGRYFSYTSAEDIFNELREASRGGIADYSGISYG
ECCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHCCCCCCCCCCCHH
RLRREGGIHWPCPESDHPGTGRLFTESFAHPDQKAALSVIPNEPPVPKEKPTADYPLYLT
HHHHCCCCCCCCCCCCCCCCCCEEHHHHCCCCCCCEEEECCCCCCCCCCCCCCCCCEEEE
TGRVMSHYLTGVQTRKSAALAARHFESFMEIHPQTAATYNIEDRVLVKIESPRGSITVRS
HHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCEEEEEEECCCCCEEEHH
KLSEQIRKDTVFVPIHWADAQNVNDLIGEALDPACKMPGFKVCAVRIIPI
HHHHHHHHCEEEEEEEECCCCCHHHHHHHHCCHHHCCCCCEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969502; 9384377; 7868621