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The map label for this gene is dnaN [H]

Identifier: 239618231

GI number: 239618231

Start: 2003959

End: 2005062

Strand: Reverse

Name: dnaN [H]

Synonym: Kole_1868

Alternate gene names: 239618231

Gene position: 2005062-2003959 (Counterclockwise)

Preceding gene: 239618233

Following gene: 239618230

Centisome position: 87.1

GC content: 42.39

Gene sequence:

>1104_bases
ATGAGATTTTCAATACCAAGGAATGAATTTTTGACACGCCTCGAATCCGTGTCAACCGCTGTTGCTTCAAAGACCGTTAA
GCCTATTCTTTCTGGTATACTCTTCAGAACAGCAGAAGACAGCAAGTTAGAACTTATCGCAACTGACCTGGAGACCTCTA
TAAAGACTTATGTTCAACCAAGCGAAATCTCCGGAACCGGGCAATTTGTCGTTGACGCGAAGGTTTTGCTTGAAATAATG
CGTAATTTACCGGATGGAAAGGTTTTCTTTGAGTTTCAGGACACGAACCTCGTCATAAGAATGGAAAAGAGTCGTTTCGT
TCTACCCACAATGTCTGGCGAAGAATTTCCAGAGGTAGAACCCGCTCAGGGTGGATGGGAACTGGAAACTTCCGTTACCG
CGCTTGAAATAATGATAGATAGGGTGCTCTTTTGTGCGGCAAAAGATGAATTTATGAGAAATCTCAACGGTGTGTACTGG
GAACTCGAAGGTGGTTATCTCCGGCTCGTAGCCGCAGATGGATTCAGACTGGCACTCGCTGAGGAAAAAACAAATCAAAA
CCTCGAAGATCATTTTCTGTTGACACTTAAAAGTATGAAAGACTTGCAATCAAGCATAAAAACAGCTGGATCAGATGACA
TGAAGATCGTTTATGATGGCACCCGGGTAGGATTCTATTTTGATGGCACAGAGATGGTGGCGAGAGTTGTTGAAGCCGAT
TTCCCGGATTATAAAAAAGTGCTTCCTAAAGCCTTCAAAACACGTGTTGTAGCTAACACCTCATCATTATCTGATGCTAT
CAGACGCGCTTCTATCGCCGCCAGGCTCGGAAGTGATTCTATTAAGATCGAGATCAAAGAGGATAACATGAAACTCATTG
CTAAGAGCCCAGATCACGGTGAATCCTCTGAAGAGCTTGAAATAAAGAAAGACGGAGAAGACCTTATCATTGCTTTCAAT
CCAAAATTCTTGCTGGAAGCAACAAAGAAGATAGATACAGAAGAAGTGGAACTCAACTTTGTGGATTCTAACAGTCCGTT
GCAGATGAATCCTGTAGATGTTGAAGGATATCTATACATTATAATGCCAATAAGGCTGATCTAA

Upstream 100 bases:

>100_bases
CGAATTTTCATGCTAACCTCCTTTATAAGGGTTCGTTGGAATTATACCCTCTGTTAGGTTAAAATATAAAGGTAGAAGAT
GGACGTTTGGAGGTGTCTAA

Downstream 100 bases:

>100_bases
ATGTATCGGGTAGGAATTGGATTCGATGCTCACCCTTTAGAAGCAAACAAAAAAGGATTATATCTTGGGGGTGTAAAAGT
ATCTGAGGAATATTCCTCAA

Product: DNA polymerase III, beta subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 367; Mature: 367

Protein sequence:

>367_residues
MRFSIPRNEFLTRLESVSTAVASKTVKPILSGILFRTAEDSKLELIATDLETSIKTYVQPSEISGTGQFVVDAKVLLEIM
RNLPDGKVFFEFQDTNLVIRMEKSRFVLPTMSGEEFPEVEPAQGGWELETSVTALEIMIDRVLFCAAKDEFMRNLNGVYW
ELEGGYLRLVAADGFRLALAEEKTNQNLEDHFLLTLKSMKDLQSSIKTAGSDDMKIVYDGTRVGFYFDGTEMVARVVEAD
FPDYKKVLPKAFKTRVVANTSSLSDAIRRASIAARLGSDSIKIEIKEDNMKLIAKSPDHGESSEELEIKKDGEDLIIAFN
PKFLLEATKKIDTEEVELNFVDSNSPLQMNPVDVEGYLYIIMPIRLI

Sequences:

>Translated_367_residues
MRFSIPRNEFLTRLESVSTAVASKTVKPILSGILFRTAEDSKLELIATDLETSIKTYVQPSEISGTGQFVVDAKVLLEIM
RNLPDGKVFFEFQDTNLVIRMEKSRFVLPTMSGEEFPEVEPAQGGWELETSVTALEIMIDRVLFCAAKDEFMRNLNGVYW
ELEGGYLRLVAADGFRLALAEEKTNQNLEDHFLLTLKSMKDLQSSIKTAGSDDMKIVYDGTRVGFYFDGTEMVARVVEAD
FPDYKKVLPKAFKTRVVANTSSLSDAIRRASIAARLGSDSIKIEIKEDNMKLIAKSPDHGESSEELEIKKDGEDLIIAFN
PKFLLEATKKIDTEEVELNFVDSNSPLQMNPVDVEGYLYIIMPIRLI
>Mature_367_residues
MRFSIPRNEFLTRLESVSTAVASKTVKPILSGILFRTAEDSKLELIATDLETSIKTYVQPSEISGTGQFVVDAKVLLEIM
RNLPDGKVFFEFQDTNLVIRMEKSRFVLPTMSGEEFPEVEPAQGGWELETSVTALEIMIDRVLFCAAKDEFMRNLNGVYW
ELEGGYLRLVAADGFRLALAEEKTNQNLEDHFLLTLKSMKDLQSSIKTAGSDDMKIVYDGTRVGFYFDGTEMVARVVEAD
FPDYKKVLPKAFKTRVVANTSSLSDAIRRASIAARLGSDSIKIEIKEDNMKLIAKSPDHGESSEELEIKKDGEDLIIAFN
PKFLLEATKKIDTEEVELNFVDSNSPLQMNPVDVEGYLYIIMPIRLI

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The beta chain is required for initiation of replication once it is clamped

COG id: COG0592

COG function: function code L; DNA polymerase sliding clamp subunit (PCNA homolog)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790136, Length=371, Percent_Identity=27.7628032345013, Blast_Score=162, Evalue=3e-41,

Paralogues:

None

Copy number: 5954 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001001
- InterPro:   IPR022635
- InterPro:   IPR022637
- InterPro:   IPR022634 [H]

Pfam domain/function: PF00712 DNA_pol3_beta; PF02767 DNA_pol3_beta_2; PF02768 DNA_pol3_beta_3 [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 41255; Mature: 41255

Theoretical pI: Translated: 4.51; Mature: 4.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRFSIPRNEFLTRLESVSTAVASKTVKPILSGILFRTAEDSKLELIATDLETSIKTYVQP
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCEEEEEECHHHHHHHHCCC
SEISGTGQFVVDAKVLLEIMRNLPDGKVFFEFQDTNLVIRMEKSRFVLPTMSGEEFPEVE
CCCCCCCEEEEEHHHHHHHHHCCCCCEEEEEEECCEEEEEEECCEEEEECCCCCCCCCCC
PAQGGWELETSVTALEIMIDRVLFCAAKDEFMRNLNGVYWELEGGYLRLVAADGFRLALA
CCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCEEEEEEECCEEEEEE
EEKTNQNLEDHFLLTLKSMKDLQSSIKTAGSDDMKIVYDGTRVGFYFDGTEMVARVVEAD
CCCCCCCCCHHHEEHHHHHHHHHHHHHHCCCCCEEEEEECEEEEEEECCHHHHHHHHHCC
FPDYKKVLPKAFKTRVVANTSSLSDAIRRASIAARLGSDSIKIEIKEDNMKLIAKSPDHG
CCHHHHHHHHHHHHEEEECCHHHHHHHHHHHHHHHCCCCEEEEEEEECCEEEEEECCCCC
ESSEELEIKKDGEDLIIAFNPKFLLEATKKIDTEEVELNFVDSNSPLQMNPVDVEGYLYI
CCCCCEEEEECCCEEEEEECCHHHHHHHHCCCCCEEEEEEECCCCCEEECCCCCCCEEEE
IMPIRLI
EEEEEEC
>Mature Secondary Structure
MRFSIPRNEFLTRLESVSTAVASKTVKPILSGILFRTAEDSKLELIATDLETSIKTYVQP
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCEEEEEECHHHHHHHHCCC
SEISGTGQFVVDAKVLLEIMRNLPDGKVFFEFQDTNLVIRMEKSRFVLPTMSGEEFPEVE
CCCCCCCEEEEEHHHHHHHHHCCCCCEEEEEEECCEEEEEEECCEEEEECCCCCCCCCCC
PAQGGWELETSVTALEIMIDRVLFCAAKDEFMRNLNGVYWELEGGYLRLVAADGFRLALA
CCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCEEEEEEECCEEEEEE
EEKTNQNLEDHFLLTLKSMKDLQSSIKTAGSDDMKIVYDGTRVGFYFDGTEMVARVVEAD
CCCCCCCCCHHHEEHHHHHHHHHHHHHHCCCCCEEEEEECEEEEEEECCHHHHHHHHHCC
FPDYKKVLPKAFKTRVVANTSSLSDAIRRASIAARLGSDSIKIEIKEDNMKLIAKSPDHG
CCHHHHHHHHHHHHEEEECCHHHHHHHHHHHHHHHCCCCEEEEEEEECCEEEEEECCCCC
ESSEELEIKKDGEDLIIAFNPKFLLEATKKIDTEEVELNFVDSNSPLQMNPVDVEGYLYI
CCCCCEEEEECCCEEEEEECCHHHHHHHHCCCCCEEEEEEECCCCCEEECCCCCCCEEEE
IMPIRLI
EEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]