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The map label for this gene is ispF

Identifier: 239618230

GI number: 239618230

Start: 2003488

End: 2003958

Strand: Reverse

Name: ispF

Synonym: Kole_1867

Alternate gene names: 239618230

Gene position: 2003958-2003488 (Counterclockwise)

Preceding gene: 239618231

Following gene: 239618229

Centisome position: 87.05

GC content: 36.52

Gene sequence:

>471_bases
ATGTATCGGGTAGGAATTGGATTCGATGCTCACCCTTTAGAAGCAAACAAAAAAGGATTATATCTTGGGGGTGTAAAAGT
ATCTGAGGAATATTCCTCAATTGGCCATTCCGATGGCGATGCATTGATTCATGCCATTGTAGATGCAATATTAGGAGCCA
CGAATGCCGGCAATATAGGTATCTGGTTTCCGGAAAATGAAGAAAATCGCGGAAGGAGAAGTACTGAGTTCCTTGAAATA
ATACGAAAAAAGATATTAGCAGGAAAATACGAGATACTGAATATCGATTCTGTGATTATTATAGACAAAGTCAGAATGAA
TCCTCATATAGAAAACATACGACTAAAACTTGCAAGAATTCTTGGCATAAGTAAAGGGAATATAAATGTGAAACCAAAAT
CTGGAAATACTTTGTATGGAAATTCAGTGTCTGTATATGCTGTGTGTATGTTAAAAAAGGTGGGGACATAG

Upstream 100 bases:

>100_bases
AGTGGAACTCAACTTTGTGGATTCTAACAGTCCGTTGCAGATGAATCCTGTAGATGTTGAAGGATATCTATACATTATAA
TGCCAATAAGGCTGATCTAA

Downstream 100 bases:

>100_bases
TATGCCGGAATATAGGTACGAAATAATCACCAAAGATGGAAAAACAGAAAGAGGAAAAATTAGCGCAGCTTCCCAACTTG
AAGCGTTGCAACTATTGAAT

Product: 2C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Products: NA

Alternate protein names: MECDP-synthase; MECPS

Number of amino acids: Translated: 156; Mature: 156

Protein sequence:

>156_residues
MYRVGIGFDAHPLEANKKGLYLGGVKVSEEYSSIGHSDGDALIHAIVDAILGATNAGNIGIWFPENEENRGRRSTEFLEI
IRKKILAGKYEILNIDSVIIIDKVRMNPHIENIRLKLARILGISKGNINVKPKSGNTLYGNSVSVYAVCMLKKVGT

Sequences:

>Translated_156_residues
MYRVGIGFDAHPLEANKKGLYLGGVKVSEEYSSIGHSDGDALIHAIVDAILGATNAGNIGIWFPENEENRGRRSTEFLEI
IRKKILAGKYEILNIDSVIIIDKVRMNPHIENIRLKLARILGISKGNINVKPKSGNTLYGNSVSVYAVCMLKKVGT
>Mature_156_residues
MYRVGIGFDAHPLEANKKGLYLGGVKVSEEYSSIGHSDGDALIHAIVDAILGATNAGNIGIWFPENEENRGRRSTEFLEI
IRKKILAGKYEILNIDSVIIIDKVRMNPHIENIRLKLARILGISKGNINVKPKSGNTLYGNSVSVYAVCMLKKVGT

Specific function: Converts 4-diphosphocytidyl-2-C-methyl-D-erythritol 2- phosphate into 2-C-methyl-D-erythritol 2,4-cyclodiphosphate (MECDP) and CMP

COG id: COG0245

COG function: function code I; 2C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispF family

Homologues:

Organism=Escherichia coli, GI1789103, Length=157, Percent_Identity=35.6687898089172, Blast_Score=87, Evalue=4e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPF_KOSOT (C5CGG7)

Other databases:

- EMBL:   CP001634
- RefSeq:   YP_002941552.1
- GeneID:   7967789
- GenomeReviews:   CP001634_GR
- KEGG:   kol:Kole_1867
- HAMAP:   MF_00107
- InterPro:   IPR003526
- InterPro:   IPR020555
- Gene3D:   G3DSA:3.30.1330.50
- TIGRFAMs:   TIGR00151

Pfam domain/function: PF02542 YgbB; SSF69765 YgbB_synth

EC number: =4.6.1.12

Molecular weight: Translated: 17106; Mature: 17106

Theoretical pI: Translated: 9.78; Mature: 9.78

Prosite motif: PS01350 ISPF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYRVGIGFDAHPLEANKKGLYLGGVKVSEEYSSIGHSDGDALIHAIVDAILGATNAGNIG
CEEECCCCCCCCCCCCCCEEEECCEEECHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCEE
IWFPENEENRGRRSTEFLEIIRKKILAGKYEILNIDSVIIIDKVRMNPHIENIRLKLARI
EECCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEECCEEEEEEEEECCCCHHHHHHHHHHH
LGISKGNINVKPKSGNTLYGNSVSVYAVCMLKKVGT
HCCCCCCEEEEECCCCEEECCCHHHHHHEEHHHCCC
>Mature Secondary Structure
MYRVGIGFDAHPLEANKKGLYLGGVKVSEEYSSIGHSDGDALIHAIVDAILGATNAGNIG
CEEECCCCCCCCCCCCCCEEEECCEEECHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCEE
IWFPENEENRGRRSTEFLEIIRKKILAGKYEILNIDSVIIIDKVRMNPHIENIRLKLARI
EECCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEECCEEEEEEEEECCCCHHHHHHHHHHH
LGISKGNINVKPKSGNTLYGNSVSVYAVCMLKKVGT
HCCCCCCEEEEECCCCEEECCCHHHHHHEEHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA