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The map label for this gene is hisJ [H]

Identifier: 239618042

GI number: 239618042

Start: 1805707

End: 1806486

Strand: Direct

Name: hisJ [H]

Synonym: Kole_1671

Alternate gene names: 239618042

Gene position: 1805707-1806486 (Clockwise)

Preceding gene: 239618039

Following gene: 239618043

Centisome position: 78.44

GC content: 43.08

Gene sequence:

>780_bases
GTGGTGAGGAGAACAACTTTCTTGGTAGTCGTTGTAAGTATTCTTTTGGTGAGCTTGACGTTCGGAAGCTTGATCGATGA
AATCAAAGAACGAGGTGTGTTGAGAGTAGGTCAGGACGCCGGGTATATGCCCTTGTATGGAACCGACATGTACGGTAACC
GTATAGGACTTGAAGTTGACGTTCTCAAGATTATGGCAACACTTCTTGGTGTTAGACTCGAATTCGTGGTTGTGAACTGG
GATGGCATTATTCCTGCGTTGCTGTCTGAAAAATTCGACATCATCTGGTCCGGGATGACAATAACAGATGAGCGGGCTAA
AAAAGTTGATTTCAGCGAGCCTTACCTTAAGATTGGTCAGGTTCTTGTCTACAACAACAGGAAAATTAGTTCAGTTCCTT
CTCTGGAAGAGCTGAATAATCCCTCTGTAAGAATTGCAGTGCAGCTAGGAACTACAGGAGATGAAGCGGCAAGGAGGTTG
TTCCCCAGAGCAAAAATTTTAACCTTTGACTCCATGGATGAAGCAGCTTTTCAGGTTGCTTCGGGTAGGGCTGACTTCAC
GGTCGTGGATTCTATATACGCACAGTACGTGGCGAAAAAATACGATCGTCTGAGTGTAGCTGATGGATTCTTGACCAGTG
AAGAACTTGGGGTTGCGATTAGAAAAAATGATCCTGAAACATTAAATTGGATCAACAATTTTATCGAAGGACTTAAATCA
TCAGGATTCATGGAACTCCTGGAAGAAAAGTGGTTTGTTGAATATGAACCAGAATTGTAA

Upstream 100 bases:

>100_bases
CGGGTTTTTCCGTCGGCAGCACAGTTTGAACGTGCTGTAGATAAAGGAGGAACGCCGGGCGAGAAATCGCCCGGCTTTTT
TTATATCAGAGGAGGTGTTT

Downstream 100 bases:

>100_bases
GTATGAGGTGAAAAGATGCTGAAGTTCATGTCCAGAGTGCTTTTAGTGATCACGGTAACTTTGATATTCTTTTCTATTTA
CGCCTCCCTTTCGAAGGTGT

Product: extracellular solute-binding protein family 3

Products: NA

Alternate protein names: HBP [H]

Number of amino acids: Translated: 259; Mature: 259

Protein sequence:

>259_residues
MVRRTTFLVVVVSILLVSLTFGSLIDEIKERGVLRVGQDAGYMPLYGTDMYGNRIGLEVDVLKIMATLLGVRLEFVVVNW
DGIIPALLSEKFDIIWSGMTITDERAKKVDFSEPYLKIGQVLVYNNRKISSVPSLEELNNPSVRIAVQLGTTGDEAARRL
FPRAKILTFDSMDEAAFQVASGRADFTVVDSIYAQYVAKKYDRLSVADGFLTSEELGVAIRKNDPETLNWINNFIEGLKS
SGFMELLEEKWFVEYEPEL

Sequences:

>Translated_259_residues
MVRRTTFLVVVVSILLVSLTFGSLIDEIKERGVLRVGQDAGYMPLYGTDMYGNRIGLEVDVLKIMATLLGVRLEFVVVNW
DGIIPALLSEKFDIIWSGMTITDERAKKVDFSEPYLKIGQVLVYNNRKISSVPSLEELNNPSVRIAVQLGTTGDEAARRL
FPRAKILTFDSMDEAAFQVASGRADFTVVDSIYAQYVAKKYDRLSVADGFLTSEELGVAIRKNDPETLNWINNFIEGLKS
SGFMELLEEKWFVEYEPEL
>Mature_259_residues
MVRRTTFLVVVVSILLVSLTFGSLIDEIKERGVLRVGQDAGYMPLYGTDMYGNRIGLEVDVLKIMATLLGVRLEFVVVNW
DGIIPALLSEKFDIIWSGMTITDERAKKVDFSEPYLKIGQVLVYNNRKISSVPSLEELNNPSVRIAVQLGTTGDEAARRL
FPRAKILTFDSMDEAAFQVASGRADFTVVDSIYAQYVAKKYDRLSVADGFLTSEELGVAIRKNDPETLNWINNFIEGLKS
SGFMELLEEKWFVEYEPEL

Specific function: Component of the high-affinity histidine permease, a binding-protein-dependent transport system. The other components are proteins hisQ, hisM, and hisP [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1788228, Length=257, Percent_Identity=31.9066147859922, Blast_Score=115, Evalue=2e-27,
Organism=Escherichia coli, GI1787085, Length=250, Percent_Identity=26.4, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1787031, Length=249, Percent_Identity=27.710843373494, Blast_Score=88, Evalue=5e-19,
Organism=Escherichia coli, GI1787088, Length=248, Percent_Identity=24.5967741935484, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI1788648, Length=230, Percent_Identity=26.9565217391304, Blast_Score=81, Evalue=8e-17,
Organism=Escherichia coli, GI1788649, Length=232, Percent_Identity=25.8620689655172, Blast_Score=79, Evalue=4e-16,

Paralogues:

None

Copy number: 1920 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1060 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 80 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015683
- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 29138; Mature: 29138

Theoretical pI: Translated: 4.52; Mature: 4.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVRRTTFLVVVVSILLVSLTFGSLIDEIKERGVLRVGQDAGYMPLYGTDMYGNRIGLEVD
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCEECCCCCCCCCCCCHH
VLKIMATLLGVRLEFVVVNWDGIIPALLSEKFDIIWSGMTITDERAKKVDFSEPYLKIGQ
HHHHHHHHHCCEEEEEEEECCCCHHHHHCCCCEEEECCCEECCHHHHCCCCCCCHHHHCC
VLVYNNRKISSVPSLEELNNPSVRIAVQLGTTGDEAARRLFPRAKILTFDSMDEAAFQVA
EEEECCCCCCCCCCHHHCCCCCEEEEEEECCCHHHHHHHHCCCCEEEEECCCHHHHHHHH
SGRADFTVVDSIYAQYVAKKYDRLSVADGFLTSEELGVAIRKNDPETLNWINNFIEGLKS
CCCCCCHHHHHHHHHHHHHHHCCHHHHCCCCCHHHHCEEEECCCCHHHHHHHHHHHHHCC
SGFMELLEEKWFVEYEPEL
CCHHHHHHHCCEEEECCCC
>Mature Secondary Structure
MVRRTTFLVVVVSILLVSLTFGSLIDEIKERGVLRVGQDAGYMPLYGTDMYGNRIGLEVD
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCEECCCCCCCCCCCCHH
VLKIMATLLGVRLEFVVVNWDGIIPALLSEKFDIIWSGMTITDERAKKVDFSEPYLKIGQ
HHHHHHHHHCCEEEEEEEECCCCHHHHHCCCCEEEECCCEECCHHHHCCCCCCCHHHHCC
VLVYNNRKISSVPSLEELNNPSVRIAVQLGTTGDEAARRLFPRAKILTFDSMDEAAFQVA
EEEECCCCCCCCCCHHHCCCCCEEEEEEECCCHHHHHHHHCCCCEEEEECCCHHHHHHHH
SGRADFTVVDSIYAQYVAKKYDRLSVADGFLTSEELGVAIRKNDPETLNWINNFIEGLKS
CCCCCCHHHHHHHHHHHHHHHCCHHHHCCCCCHHHHCEEEECCCCHHHHHHHHHHHHHCC
SGFMELLEEKWFVEYEPEL
CCHHHHHHHCCEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 1448614 [H]