Click here to switch to the map view.

The map label for this gene is glyA

Identifier: 239617830

GI number: 239617830

Start: 1554554

End: 1555822

Strand: Direct

Name: glyA

Synonym: Kole_1456

Alternate gene names: 239617830

Gene position: 1554554-1555822 (Clockwise)

Preceding gene: 239617829

Following gene: 239617831

Centisome position: 67.53

GC content: 43.74

Gene sequence:

>1269_bases
GTGTGGGAATTGCTTGCAAAGGGCGATCCCGAGGTTTATGAGATCGTTATGAAGGAGCTTGGCAGGCAGGAGGAGGGTTT
AGAGCTCATTGCTTCGGAGAATTTTGTTTCTCCTGCAGTTATGGAGGCAATGGGTTCGACTTTAACGAACAAATACGCTG
AGGGATACCCAAGGAGGCGTTATTATGGAGGTTGTAAGTTTGTTGACGAAGCTGAACAACTTGCCAGAGAACGTGTGAAG
AAACTTTTTAATTGTAAATACGCGAATGTTCAGCCTCACTCAGGTTCTCAAGCAAACATGGCAGCGTATCTTGCTGTGGC
GAATCCCGGTGATACTATTATGGGAATGTCGTTGAGCCATGGCGGACATCTAACCCACGGTTCACCGGTAAATTTTTCAG
GGAAGCTTTTCAATATAGTATCCTATGGGGTTGATCTCGAAACAGAGGTTCTCGATTATGATGAGATAGAGAGACTGGCG
CTTGAACATAAACCAAAGATCATTATTGCCGGTGGAAGTGCTTATTCAAGGATTATTGATTTTAAGCGCTTCAGGGAAAT
AGCTGATAAAGTCGGAGCTTATCTTATCGTTGATATGGCCCATTTTGCTGGGTTGGTTGCAGCCGGTCTTTATCCCAATC
CAGTGGATCATGCTCATATAGTTACTTCTACCACGCATAAGACCTTAAGGGGACCTCGAGGTGGTTTGATACTTACCAAT
GACGAGGAACTTTATAAAGCGATAAACAAAGCGGTATTTCCGGGAATTCAAGGGGGACCGTTAATGCATGTGATAGCGGC
CAAAGCGGTTGCTTTTGGTGAAGCTTTAAAGGATGAATTCAAAGAGTATCAAATGAGAATTATTACAAACGCCAAAGCCC
TGGCAAAAGAGCTGGAAAACCTTGGATTGAGAATCGTTTCCGGTGGAACAGATACCCATCTGTTCCTTGTTGATCTTAAT
CCGAAGAATGTAACCGGTAAAGCAGCCGAAAAAGCTCTTGAAAGTGCGGATATAACTGTCAACAAGAATACTATCCCGAA
GGAAACGCGTTCCCCGTTTGTTACGAGCGGTATCAGAATTGGAACGCCTGCTGTTACTACCCGAGGCATGGGTGAAAGTG
AGATGAAGGTTATTGCGGAGCTCATAGTGAAGGTTATAGATAATATTCAGGATGAAAAGGGAACAATACCGGAAGAAATA
AGAGAAGAGGTAAAAAAAGCTGTCCATGAACTCACTGAAAAGTTCCCGTTGTACAAAGATCTTACGTGA

Upstream 100 bases:

>100_bases
ACGCGCTAGCGTGCATACCGGATCTCGGATTCTCGGATCTCGGACTCTCGGTGTTTTTCCGCACAACCTACAACCAATTT
TAAATAAGGAGGTAATACCC

Downstream 100 bases:

>100_bases
ATTGAGGCGAGAGTATGAGAAACCTTATGGTTGTTAATCATCCTTTGATTCAGCATAAACTTTCTATTCTAAGGAACAAG
GATACAGGACCGAAGGAGTT

Product: serine hydroxymethyltransferase

Products: NA

Alternate protein names: SHMT; Serine methylase

Number of amino acids: Translated: 422; Mature: 422

Protein sequence:

>422_residues
MWELLAKGDPEVYEIVMKELGRQEEGLELIASENFVSPAVMEAMGSTLTNKYAEGYPRRRYYGGCKFVDEAEQLARERVK
KLFNCKYANVQPHSGSQANMAAYLAVANPGDTIMGMSLSHGGHLTHGSPVNFSGKLFNIVSYGVDLETEVLDYDEIERLA
LEHKPKIIIAGGSAYSRIIDFKRFREIADKVGAYLIVDMAHFAGLVAAGLYPNPVDHAHIVTSTTHKTLRGPRGGLILTN
DEELYKAINKAVFPGIQGGPLMHVIAAKAVAFGEALKDEFKEYQMRIITNAKALAKELENLGLRIVSGGTDTHLFLVDLN
PKNVTGKAAEKALESADITVNKNTIPKETRSPFVTSGIRIGTPAVTTRGMGESEMKVIAELIVKVIDNIQDEKGTIPEEI
REEVKKAVHELTEKFPLYKDLT

Sequences:

>Translated_422_residues
MWELLAKGDPEVYEIVMKELGRQEEGLELIASENFVSPAVMEAMGSTLTNKYAEGYPRRRYYGGCKFVDEAEQLARERVK
KLFNCKYANVQPHSGSQANMAAYLAVANPGDTIMGMSLSHGGHLTHGSPVNFSGKLFNIVSYGVDLETEVLDYDEIERLA
LEHKPKIIIAGGSAYSRIIDFKRFREIADKVGAYLIVDMAHFAGLVAAGLYPNPVDHAHIVTSTTHKTLRGPRGGLILTN
DEELYKAINKAVFPGIQGGPLMHVIAAKAVAFGEALKDEFKEYQMRIITNAKALAKELENLGLRIVSGGTDTHLFLVDLN
PKNVTGKAAEKALESADITVNKNTIPKETRSPFVTSGIRIGTPAVTTRGMGESEMKVIAELIVKVIDNIQDEKGTIPEEI
REEVKKAVHELTEKFPLYKDLT
>Mature_422_residues
MWELLAKGDPEVYEIVMKELGRQEEGLELIASENFVSPAVMEAMGSTLTNKYAEGYPRRRYYGGCKFVDEAEQLARERVK
KLFNCKYANVQPHSGSQANMAAYLAVANPGDTIMGMSLSHGGHLTHGSPVNFSGKLFNIVSYGVDLETEVLDYDEIERLA
LEHKPKIIIAGGSAYSRIIDFKRFREIADKVGAYLIVDMAHFAGLVAAGLYPNPVDHAHIVTSTTHKTLRGPRGGLILTN
DEELYKAINKAVFPGIQGGPLMHVIAAKAVAFGEALKDEFKEYQMRIITNAKALAKELENLGLRIVSGGTDTHLFLVDLN
PKNVTGKAAEKALESADITVNKNTIPKETRSPFVTSGIRIGTPAVTTRGMGESEMKVIAELIVKVIDNIQDEKGTIPEEI
REEVKKAVHELTEKFPLYKDLT

Specific function: Interconversion of serine and glycine

COG id: COG0112

COG function: function code E; Glycine/serine hydroxymethyltransferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the SHMT family

Homologues:

Organism=Homo sapiens, GI19923315, Length=403, Percent_Identity=47.1464019851117, Blast_Score=359, Evalue=2e-99,
Organism=Homo sapiens, GI261862352, Length=403, Percent_Identity=47.1464019851117, Blast_Score=359, Evalue=3e-99,
Organism=Homo sapiens, GI261862350, Length=403, Percent_Identity=47.1464019851117, Blast_Score=359, Evalue=3e-99,
Organism=Homo sapiens, GI261862348, Length=403, Percent_Identity=47.1464019851117, Blast_Score=359, Evalue=3e-99,
Organism=Homo sapiens, GI22547186, Length=416, Percent_Identity=48.3173076923077, Blast_Score=355, Evalue=6e-98,
Organism=Homo sapiens, GI261862346, Length=403, Percent_Identity=46.8982630272953, Blast_Score=346, Evalue=3e-95,
Organism=Homo sapiens, GI22547189, Length=402, Percent_Identity=46.0199004975124, Blast_Score=319, Evalue=2e-87,
Organism=Escherichia coli, GI1788902, Length=416, Percent_Identity=60.8173076923077, Blast_Score=497, Evalue=1e-142,
Organism=Caenorhabditis elegans, GI25144732, Length=439, Percent_Identity=45.5580865603645, Blast_Score=362, Evalue=1e-100,
Organism=Caenorhabditis elegans, GI25144729, Length=439, Percent_Identity=45.5580865603645, Blast_Score=362, Evalue=1e-100,
Organism=Saccharomyces cerevisiae, GI6319739, Length=438, Percent_Identity=45.662100456621, Blast_Score=381, Evalue=1e-106,
Organism=Saccharomyces cerevisiae, GI6323087, Length=418, Percent_Identity=46.88995215311, Blast_Score=372, Evalue=1e-104,
Organism=Drosophila melanogaster, GI24640005, Length=452, Percent_Identity=46.6814159292035, Blast_Score=392, Evalue=1e-109,
Organism=Drosophila melanogaster, GI221329721, Length=452, Percent_Identity=46.6814159292035, Blast_Score=390, Evalue=1e-109,

Paralogues:

None

Copy number: 3180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 12,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): GLYA_KOSOT (C5CEA8)

Other databases:

- EMBL:   CP001634
- RefSeq:   YP_002941152.1
- ProteinModelPortal:   C5CEA8
- GeneID:   7969098
- GenomeReviews:   CP001634_GR
- KEGG:   kol:Kole_1456
- ProtClustDB:   PRK00011
- GO:   GO:0005737
- HAMAP:   MF_00051_B
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- InterPro:   IPR001085
- InterPro:   IPR019798
- Gene3D:   G3DSA:3.40.640.10
- Gene3D:   G3DSA:3.90.1150.10
- PANTHER:   PTHR11680
- PIRSF:   PIRSF000412

Pfam domain/function: PF00464 SHMT; SSF53383 PyrdxlP-dep_Trfase_major

EC number: =2.1.2.1

Molecular weight: Translated: 46404; Mature: 46404

Theoretical pI: Translated: 6.39; Mature: 6.39

Prosite motif: PS00096 SHMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MWELLAKGDPEVYEIVMKELGRQEEGLELIASENFVSPAVMEAMGSTLTNKYAEGYPRRR
CCCCCCCCCHHHHHHHHHHHCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCH
YYGGCKFVDEAEQLARERVKKLFNCKYANVQPHSGSQANMAAYLAVANPGDTIMGMSLSH
HCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEEECCCCCEEEEEECC
GGHLTHGSPVNFSGKLFNIVSYGVDLETEVLDYDEIERLALEHKPKIIIAGGSAYSRIID
CCCCCCCCCCCCCCHHEEHHHHCCCCCHHHCCHHHHHHHHHHCCCEEEEECCHHHHHHHH
FKRFREIADKVGAYLIVDMAHFAGLVAAGLYPNPVDHAHIVTSTTHKTLRGPRGGLILTN
HHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCHHHHCCCCCCEEEEC
DEELYKAINKAVFPGIQGGPLMHVIAAKAVAFGEALKDEFKEYQMRIITNAKALAKELEN
CHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LGLRIVSGGTDTHLFLVDLNPKNVTGKAAEKALESADITVNKNTIPKETRSPFVTSGIRI
CCEEEEECCCCEEEEEEECCCCCCCCHHHHHHHHHCCEEECCCCCCCCCCCCCHHCCEEE
GTPAVTTRGMGESEMKVIAELIVKVIDNIQDEKGTIPEEIREEVKKAVHELTEKFPLYKD
CCCCCEECCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCC
LT
CC
>Mature Secondary Structure
MWELLAKGDPEVYEIVMKELGRQEEGLELIASENFVSPAVMEAMGSTLTNKYAEGYPRRR
CCCCCCCCCHHHHHHHHHHHCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCH
YYGGCKFVDEAEQLARERVKKLFNCKYANVQPHSGSQANMAAYLAVANPGDTIMGMSLSH
HCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEEECCCCCEEEEEECC
GGHLTHGSPVNFSGKLFNIVSYGVDLETEVLDYDEIERLALEHKPKIIIAGGSAYSRIID
CCCCCCCCCCCCCCHHEEHHHHCCCCCHHHCCHHHHHHHHHHCCCEEEEECCHHHHHHHH
FKRFREIADKVGAYLIVDMAHFAGLVAAGLYPNPVDHAHIVTSTTHKTLRGPRGGLILTN
HHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCHHHHCCCCCCEEEEC
DEELYKAINKAVFPGIQGGPLMHVIAAKAVAFGEALKDEFKEYQMRIITNAKALAKELEN
CHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LGLRIVSGGTDTHLFLVDLNPKNVTGKAAEKALESADITVNKNTIPKETRSPFVTSGIRI
CCEEEEECCCCEEEEEEECCCCCCCCHHHHHHHHHCCEEECCCCCCCCCCCCCHHCCEEE
GTPAVTTRGMGESEMKVIAELIVKVIDNIQDEKGTIPEEIREEVKKAVHELTEKFPLYKD
CCCCCEECCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCC
LT
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA