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The map label for this gene is pulA [H]

Identifier: 239617829

GI number: 239617829

Start: 1552136

End: 1554217

Strand: Direct

Name: pulA [H]

Synonym: Kole_1455

Alternate gene names: 239617829

Gene position: 1552136-1554217 (Clockwise)

Preceding gene: 239617828

Following gene: 239617830

Centisome position: 67.42

GC content: 40.59

Gene sequence:

>2082_bases
GTGGCGGACTTTGCGGTAATAAATTCACGTAGAGAAATACGCCTTTTTTCTTCTTCGATACCTTCTATTCTTCTGATAAA
CGGCAGGAGAGCTTCTTTCATTATTGATGAGTTTAATGGAGTGAAATTGATAAAAGTCGATTTTGATCTCTCCGTTTATG
ACGAAGTTATCATCGAACTGTCCAATGGTTTATCGGTACCAGCTTATCCTGTTGGGTTAATTGATGAATCCGAAATTACT
TATGATGATTGGCTCGGTTATAAAATTGAGGATGACAGCATTGTTTTAAAGGTCTGGGCACCGGGACCCCAGAGGCTTTT
TATCGAAATTTTCGAATCTGAGAGTAGCGCAGATTTGCCAGTTGTTGTTCCTATGGAACTCGATAAAGAAACTGGTGTCT
GGTCTGTGAAAATCAGTAAAAAATTTCTTAATTCCGCTTATCGTTATAGGATAGAGCGTTTTGGAAAAATCCTTTACTCT
CCGGATCCATATGCCCCAGCTGCTACGGTTAACGGTAAACTTTCCTATATCCTCAAATTTGATGAATTTGTTCCGGAAGG
TTGGGAAAGTGATGAAGGTCCGAAATTGGATTCTCCTGTCGATGCAATTATCTATGAAGCTCATGTCAAAGACCTTTCGA
CTTCCTGGACCGCTAACTTTCAAGACCGTGGAAAATTTGTGTCTCTGTTAGAAGCTGACGCTTCCACTGTTTTGGGTGAT
AAAGCAGGTATAGCTCACATTCGCGAACTTGGTGCGACCCATATCCAGTTTCTGCCTCTTCAGGACTTTGGAAGTGTTGA
TGAGGTTGATGACAACGCTTATAACTGGGGTTATGACCCAATGCTTTATAACGTTCCAGAAGGTTCGTATTCAACCGATC
CATGCTCTCCTGAAAGAAGAATCCTGGAATTTAGAAAACTGGTAAGTGCAGTACACAAAAACGGTCTAGGTGTAATACTC
GACGTGGTTTACAACCACACTTATGTTATTGATACTCCTTTCAACAGACTTGTTCCCTATTACTATTACAGACTCAAGGA
AAATGGCGAGTTTTCGAATGGTTCTGGTTGTGGCAATGAACTTGCGACAGAAAGAACTATGGTCAGAAAATTCATAATCG
ATTCATTGAAGCACTGGGTAAGGAATTATCATGTAGATGGTTTTCGTTTTGATCTAATGGCCCTTCTGGGAAAAAGTACT
GTACTAGAAATTGAGCGAGAATTGAGACAGGTAAAAGAAGATCTTCTTCTTTACGGTGAACCATGGTCAGCTGCTTCGAG
TTCTATAAAAGATGTGTGTCTCACAAAGGGTGAGCAAAGAAATATCAACATTGCGGTTTTCAACGATGATCTAAGAAATG
CTCTCAAAGGCTATCCTGATGATGAATCAAAAGGATTTGTTTCCGGCGAACGCGGTTGGGAAACGGGCATTGTGATAGGT
ATGCTGGGGGAGATCGATTACTCGCATGTTTTCAAGGGGTTTACTGCACACCCCACAGAAACCATTAATTATGCTTCTGC
TCATGATAACCTCACCCTTTACGATAAACTTATCAAAAGCGTTCCAAAAATATCTTTCGAAGAAAGAAAAAGAATGGCAG
CTCTGGCCCTCAGTATAATACTCACTTCTCAGGGAATAGCATTCATACATGCAGGTTCTGAGATGCTCAGAACAAAGTTT
TTAGAAGAAAACAGTTATCGCTCTGGTAGCTTGATCAACGAACTGAATTATCTTCAAAAACGGATATACAGGGATTTTCA
CGAATATATACGGGGGTTGATTCTGCTCAGGAAGAACCATCTTCTTTTCAGACTTCGTACTGCAGATGAGGTAAGAAGAC
ATGTTGAAGTACTCCACATTGCCGATGGTTTTGTTTCAGTAAGATTGAAAGATGATCAAGAAGAGATTTTAGTAGCGCAC
AACGCTAGAAATGAAGCGGTTTCTTTAAACATTAGCGGGACTTGGAAGTTGCTGGTGTACGATTGTAAAATTGATCTGGA
TGGACAAAGAAAGGTACTGGATACCATTAAGGTGGAGAGATTGTCTACGACAGTGGCGATCAGGATGAAAGATGAGAGAT
GA

Upstream 100 bases:

>100_bases
ATACGATGTGGAATTAAAAAAACTTTTTGAATGAGTGTTGGGATTTGATGTTCAATGTTATCTGACCCCCGTAAAGGGGG
TCAGTTTTAGGAGGAATTCT

Downstream 100 bases:

>100_bases
GAGGCCGAGAACCGAGAGGGTGAGAAGGCGAGAGAGCGAGATCCGAGAATCCGAGAATCGGTATGCTTGCAAGTGCGTAT
CAACTGCGAAGCAGTTCGAA

Product: pullulanase, type I

Products: NA

Alternate protein names: Alpha-dextrin endo-1,6-alpha-glucosidase; Pullulan 6-glucanohydrolase [H]

Number of amino acids: Translated: 693; Mature: 692

Protein sequence:

>693_residues
MADFAVINSRREIRLFSSSIPSILLINGRRASFIIDEFNGVKLIKVDFDLSVYDEVIIELSNGLSVPAYPVGLIDESEIT
YDDWLGYKIEDDSIVLKVWAPGPQRLFIEIFESESSADLPVVVPMELDKETGVWSVKISKKFLNSAYRYRIERFGKILYS
PDPYAPAATVNGKLSYILKFDEFVPEGWESDEGPKLDSPVDAIIYEAHVKDLSTSWTANFQDRGKFVSLLEADASTVLGD
KAGIAHIRELGATHIQFLPLQDFGSVDEVDDNAYNWGYDPMLYNVPEGSYSTDPCSPERRILEFRKLVSAVHKNGLGVIL
DVVYNHTYVIDTPFNRLVPYYYYRLKENGEFSNGSGCGNELATERTMVRKFIIDSLKHWVRNYHVDGFRFDLMALLGKST
VLEIERELRQVKEDLLLYGEPWSAASSSIKDVCLTKGEQRNINIAVFNDDLRNALKGYPDDESKGFVSGERGWETGIVIG
MLGEIDYSHVFKGFTAHPTETINYASAHDNLTLYDKLIKSVPKISFEERKRMAALALSIILTSQGIAFIHAGSEMLRTKF
LEENSYRSGSLINELNYLQKRIYRDFHEYIRGLILLRKNHLLFRLRTADEVRRHVEVLHIADGFVSVRLKDDQEEILVAH
NARNEAVSLNISGTWKLLVYDCKIDLDGQRKVLDTIKVERLSTTVAIRMKDER

Sequences:

>Translated_693_residues
MADFAVINSRREIRLFSSSIPSILLINGRRASFIIDEFNGVKLIKVDFDLSVYDEVIIELSNGLSVPAYPVGLIDESEIT
YDDWLGYKIEDDSIVLKVWAPGPQRLFIEIFESESSADLPVVVPMELDKETGVWSVKISKKFLNSAYRYRIERFGKILYS
PDPYAPAATVNGKLSYILKFDEFVPEGWESDEGPKLDSPVDAIIYEAHVKDLSTSWTANFQDRGKFVSLLEADASTVLGD
KAGIAHIRELGATHIQFLPLQDFGSVDEVDDNAYNWGYDPMLYNVPEGSYSTDPCSPERRILEFRKLVSAVHKNGLGVIL
DVVYNHTYVIDTPFNRLVPYYYYRLKENGEFSNGSGCGNELATERTMVRKFIIDSLKHWVRNYHVDGFRFDLMALLGKST
VLEIERELRQVKEDLLLYGEPWSAASSSIKDVCLTKGEQRNINIAVFNDDLRNALKGYPDDESKGFVSGERGWETGIVIG
MLGEIDYSHVFKGFTAHPTETINYASAHDNLTLYDKLIKSVPKISFEERKRMAALALSIILTSQGIAFIHAGSEMLRTKF
LEENSYRSGSLINELNYLQKRIYRDFHEYIRGLILLRKNHLLFRLRTADEVRRHVEVLHIADGFVSVRLKDDQEEILVAH
NARNEAVSLNISGTWKLLVYDCKIDLDGQRKVLDTIKVERLSTTVAIRMKDER
>Mature_692_residues
ADFAVINSRREIRLFSSSIPSILLINGRRASFIIDEFNGVKLIKVDFDLSVYDEVIIELSNGLSVPAYPVGLIDESEITY
DDWLGYKIEDDSIVLKVWAPGPQRLFIEIFESESSADLPVVVPMELDKETGVWSVKISKKFLNSAYRYRIERFGKILYSP
DPYAPAATVNGKLSYILKFDEFVPEGWESDEGPKLDSPVDAIIYEAHVKDLSTSWTANFQDRGKFVSLLEADASTVLGDK
AGIAHIRELGATHIQFLPLQDFGSVDEVDDNAYNWGYDPMLYNVPEGSYSTDPCSPERRILEFRKLVSAVHKNGLGVILD
VVYNHTYVIDTPFNRLVPYYYYRLKENGEFSNGSGCGNELATERTMVRKFIIDSLKHWVRNYHVDGFRFDLMALLGKSTV
LEIERELRQVKEDLLLYGEPWSAASSSIKDVCLTKGEQRNINIAVFNDDLRNALKGYPDDESKGFVSGERGWETGIVIGM
LGEIDYSHVFKGFTAHPTETINYASAHDNLTLYDKLIKSVPKISFEERKRMAALALSIILTSQGIAFIHAGSEMLRTKFL
EENSYRSGSLINELNYLQKRIYRDFHEYIRGLILLRKNHLLFRLRTADEVRRHVEVLHIADGFVSVRLKDDQEEILVAHN
ARNEAVSLNISGTWKLLVYDCKIDLDGQRKVLDTIKVERLSTTVAIRMKDER

Specific function: Hydrolyzes The Alpha-1,6-Glucosidic Linkages In Glycogen Which Has First Been Partially Depolymerized By Phosphorylase. Shows Only Very Little Activity With Native Glycogen. [C]

COG id: COG1523

COG function: function code G; Type II secretory pathway, pullulanase PulA and related glycosidases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Escherichia coli, GI2367229, Length=464, Percent_Identity=28.2327586206897, Blast_Score=132, Evalue=5e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006047
- InterPro:   IPR004193
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR013783
- InterPro:   IPR014756
- InterPro:   IPR005323
- InterPro:   IPR011840 [H]

Pfam domain/function: PF00128 Alpha-amylase; PF02922 CBM_48; PF03714 PUD [H]

EC number: =3.2.1.41 [H]

Molecular weight: Translated: 78921; Mature: 78789

Theoretical pI: Translated: 5.24; Mature: 5.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADFAVINSRREIRLFSSSIPSILLINGRRASFIIDEFNGVKLIKVDFDLSVYDEVIIEL
CCCCEEECCCCEEEEECCCCCEEEEECCCCEEEEEECCCCEEEEEEECCHHHHHHHHHHH
SNGLSVPAYPVGLIDESEITYDDWLGYKIEDDSIVLKVWAPGPQRLFIEIFESESSADLP
CCCCCCCCCEECCCCCCCCCHHHCCCEEECCCCEEEEEECCCCCEEEEEEEECCCCCCCC
VVVPMELDKETGVWSVKISKKFLNSAYRYRIERFGKILYSPDPYAPAATVNGKLSYILKF
EEEEEEECCCCCEEEEEEHHHHHHHHHHHHHHHHHHEEECCCCCCCCEEECCCEEEEEEE
DEFVPEGWESDEGPKLDSPVDAIIYEAHVKDLSTSWTANFQDRGKFVSLLEADASTVLGD
HHHCCCCCCCCCCCCCCCCHHHHEEHHHHHHCCCCCCCCCHHCCCEEEHHHCCCHHHCCC
KAGIAHIRELGATHIQFLPLQDFGSVDEVDDNAYNWGYDPMLYNVPEGSYSTDPCSPERR
CCHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCHHHH
ILEFRKLVSAVHKNGLGVILDVVYNHTYVIDTPFNRLVPYYYYRLKENGEFSNGSGCGNE
HHHHHHHHHHHHCCCCEEEEEEHHCCEEEEECCHHHCCCEEEEEEECCCCCCCCCCCCHH
LATERTMVRKFIIDSLKHWVRNYHVDGFRFDLMALLGKSTVLEIERELRQVKEDLLLYGE
HHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHEEECC
PWSAASSSIKDVCLTKGEQRNINIAVFNDDLRNALKGYPDDESKGFVSGERGWETGIVIG
CCCHHHHHHHHHHHCCCCCCEEEEEEECHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEE
MLGEIDYSHVFKGFTAHPTETINYASAHDNLTLYDKLIKSVPKISFEERKRMAALALSII
EECCCCHHHHHCCCCCCCCCCCEEECCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
LTSQGIAFIHAGSEMLRTKFLEENSYRSGSLINELNYLQKRIYRDFHEYIRGLILLRKNH
HCCCCEEEEECCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEECCC
LLFRLRTADEVRRHVEVLHIADGFVSVRLKDDQEEILVAHNARNEAVSLNISGTWKLLVY
EEEEECCHHHHHHHHEEEEEECCEEEEEEECCCCEEEEEECCCCCEEEEEECCEEEEEEE
DCKIDLDGQRKVLDTIKVERLSTTVAIRMKDER
EEEECCCCCHHHHHHHHHHEEEEEEEEEEECCC
>Mature Secondary Structure 
ADFAVINSRREIRLFSSSIPSILLINGRRASFIIDEFNGVKLIKVDFDLSVYDEVIIEL
CCCEEECCCCEEEEECCCCCEEEEECCCCEEEEEECCCCEEEEEEECCHHHHHHHHHHH
SNGLSVPAYPVGLIDESEITYDDWLGYKIEDDSIVLKVWAPGPQRLFIEIFESESSADLP
CCCCCCCCCEECCCCCCCCCHHHCCCEEECCCCEEEEEECCCCCEEEEEEEECCCCCCCC
VVVPMELDKETGVWSVKISKKFLNSAYRYRIERFGKILYSPDPYAPAATVNGKLSYILKF
EEEEEEECCCCCEEEEEEHHHHHHHHHHHHHHHHHHEEECCCCCCCCEEECCCEEEEEEE
DEFVPEGWESDEGPKLDSPVDAIIYEAHVKDLSTSWTANFQDRGKFVSLLEADASTVLGD
HHHCCCCCCCCCCCCCCCCHHHHEEHHHHHHCCCCCCCCCHHCCCEEEHHHCCCHHHCCC
KAGIAHIRELGATHIQFLPLQDFGSVDEVDDNAYNWGYDPMLYNVPEGSYSTDPCSPERR
CCHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCHHHH
ILEFRKLVSAVHKNGLGVILDVVYNHTYVIDTPFNRLVPYYYYRLKENGEFSNGSGCGNE
HHHHHHHHHHHHCCCCEEEEEEHHCCEEEEECCHHHCCCEEEEEEECCCCCCCCCCCCHH
LATERTMVRKFIIDSLKHWVRNYHVDGFRFDLMALLGKSTVLEIERELRQVKEDLLLYGE
HHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHEEECC
PWSAASSSIKDVCLTKGEQRNINIAVFNDDLRNALKGYPDDESKGFVSGERGWETGIVIG
CCCHHHHHHHHHHHCCCCCCEEEEEEECHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEE
MLGEIDYSHVFKGFTAHPTETINYASAHDNLTLYDKLIKSVPKISFEERKRMAALALSII
EECCCCHHHHHCCCCCCCCCCCEEECCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
LTSQGIAFIHAGSEMLRTKFLEENSYRSGSLINELNYLQKRIYRDFHEYIRGLILLRKNH
HCCCCEEEEECCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEECCC
LLFRLRTADEVRRHVEVLHIADGFVSVRLKDDQEEILVAHNARNEAVSLNISGTWKLLVY
EEEEECCHHHHHHHHEEEEEECCEEEEEEECCCCEEEEEECCCCCEEEEEECCEEEEEEE
DCKIDLDGQRKVLDTIKVERLSTTVAIRMKDER
EEEECCCCCHHHHHHHHHHEEEEEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9453151; 10360571 [H]