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The map label for this gene is proB

Identifier: 239617794

GI number: 239617794

Start: 1512325

End: 1513404

Strand: Direct

Name: proB

Synonym: Kole_1419

Alternate gene names: 239617794

Gene position: 1512325-1513404 (Clockwise)

Preceding gene: 239617793

Following gene: 239617798

Centisome position: 65.69

GC content: 43.43

Gene sequence:

>1080_bases
ATGGCAAAGATAACCATAAAAGTTGGTAGCAACCTGTTAGTGCAACAAGACGGTCAGCTAGATAAACGATATATAGTAGA
ACTCTGTCGTGAAATCGGTAATTTAATGTCTGAAGGACATCAGGTAGTTCTAGTGTCTTCAGGTGCACGTGCCGCAGGGT
ATGGGTATCTCAACAAGAGCAATGCACAGGAAGCGGATCTCTACATGAAGCAAGCCTTGTGCGCTGTTGGACAGGTGCAG
CTTATGAAACTCTATGAAAGTGCTATGAGCTTTTACGGGATCAAAGTAGCCCAAATCCTGCTTACACGCGAAGACTTCAG
TCATCGCAAACGTTTTTTGAATCTTCGCAACACGTTAATCGGTCTCACAGAAATGGGAATTCTGCCCATAGTAAATGAGA
ACGACTCTGTGGCAACCGAGGAAATCATGTTCGGGGATAACGATGTTCTTGCCTCAATGTTTGCAATTGGATGGAATGCC
GATTATCTCTTATTGATGACCTCCGTAGATGGAGTTATAGACCAAAATGGGAAAGTAATACCTTTTTACCGTGAGGATAC
TACAAACATGGCGATTGCCAAAAACGCCTCTTCACGCTGGGGATCTGGGGGAATCACTTCCAAAATCCGTGCAGCCAGAG
CTGCAGCCGCAGCGGGGATACAAACTTCTATTTGTAATGGGAAAAAACTAGAAAATATTGCACAATTCGTCCTCACCGGC
CAAACTGGAACCGTATTCGAAAGGGTTGGGCCTATAAAAGCCAAAAAAGCGTGGATTGGATTCCTTTCAAAACCAAAAGG
CAGTATCTTCATCAATGAAGGAGCAAAAATAGCAATCGAAAACAACCGCAGCCTCCTTCCCGTTGGTGTTGTTCACATAG
AAGGGGACTTTCAAGCCGGGGATGTTGTAGAAATCAGATTAGATGATGGTACGTTCCTCGGAAAAGGAATTATAAATTTC
TCATCCGCAGAAGCAAAAAAAATAGTTGGTCTAAAGAGCGACCAACTGGAAGATGTGCTCGGATATTCTTGCAGTAAGGT
TCTTATACATATCGATAACCTATGGAAACGGGATAATTGA

Upstream 100 bases:

>100_bases
TCAGTACGCAGAGACTCCACGCAAGAGGGCCTGTAGGACTCGAAGGCTTGACCACTTATAAATACGTGATTCTTGGTGAT
TACAACGTGAGGAGATAATT

Downstream 100 bases:

>100_bases
TAATTGCGATGTTGGAATCCAGTAGAGCTGACCGATGCTAGGATTGCCTTCGACAATGATGTTACTTCTCGCTTCGCTCA
TCATCGTTGTCGGTTGTGGG

Product: gamma-glutamyl kinase

Products: NA

Alternate protein names: Gamma-glutamyl kinase; GK

Number of amino acids: Translated: 359; Mature: 358

Protein sequence:

>359_residues
MAKITIKVGSNLLVQQDGQLDKRYIVELCREIGNLMSEGHQVVLVSSGARAAGYGYLNKSNAQEADLYMKQALCAVGQVQ
LMKLYESAMSFYGIKVAQILLTREDFSHRKRFLNLRNTLIGLTEMGILPIVNENDSVATEEIMFGDNDVLASMFAIGWNA
DYLLLMTSVDGVIDQNGKVIPFYREDTTNMAIAKNASSRWGSGGITSKIRAARAAAAAGIQTSICNGKKLENIAQFVLTG
QTGTVFERVGPIKAKKAWIGFLSKPKGSIFINEGAKIAIENNRSLLPVGVVHIEGDFQAGDVVEIRLDDGTFLGKGIINF
SSAEAKKIVGLKSDQLEDVLGYSCSKVLIHIDNLWKRDN

Sequences:

>Translated_359_residues
MAKITIKVGSNLLVQQDGQLDKRYIVELCREIGNLMSEGHQVVLVSSGARAAGYGYLNKSNAQEADLYMKQALCAVGQVQ
LMKLYESAMSFYGIKVAQILLTREDFSHRKRFLNLRNTLIGLTEMGILPIVNENDSVATEEIMFGDNDVLASMFAIGWNA
DYLLLMTSVDGVIDQNGKVIPFYREDTTNMAIAKNASSRWGSGGITSKIRAARAAAAAGIQTSICNGKKLENIAQFVLTG
QTGTVFERVGPIKAKKAWIGFLSKPKGSIFINEGAKIAIENNRSLLPVGVVHIEGDFQAGDVVEIRLDDGTFLGKGIINF
SSAEAKKIVGLKSDQLEDVLGYSCSKVLIHIDNLWKRDN
>Mature_358_residues
AKITIKVGSNLLVQQDGQLDKRYIVELCREIGNLMSEGHQVVLVSSGARAAGYGYLNKSNAQEADLYMKQALCAVGQVQL
MKLYESAMSFYGIKVAQILLTREDFSHRKRFLNLRNTLIGLTEMGILPIVNENDSVATEEIMFGDNDVLASMFAIGWNAD
YLLLMTSVDGVIDQNGKVIPFYREDTTNMAIAKNASSRWGSGGITSKIRAARAAAAAGIQTSICNGKKLENIAQFVLTGQ
TGTVFERVGPIKAKKAWIGFLSKPKGSIFINEGAKIAIENNRSLLPVGVVHIEGDFQAGDVVEIRLDDGTFLGKGIINFS
SAEAKKIVGLKSDQLEDVLGYSCSKVLIHIDNLWKRDN

Specific function: Catalyzes the transfer of a phosphate group to glutamate to form glutamate 5-phosphate which rapidly cyclizes to 5- oxoproline

COG id: COG0263

COG function: function code E; Glutamate 5-kinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain

Homologues:

Organism=Homo sapiens, GI62912457, Length=288, Percent_Identity=29.8611111111111, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI21361368, Length=290, Percent_Identity=29.6551724137931, Blast_Score=100, Evalue=4e-21,
Organism=Escherichia coli, GI1786437, Length=363, Percent_Identity=38.2920110192837, Blast_Score=231, Evalue=4e-62,
Organism=Caenorhabditis elegans, GI17569969, Length=263, Percent_Identity=30.7984790874525, Blast_Score=114, Evalue=6e-26,
Organism=Caenorhabditis elegans, GI17569967, Length=263, Percent_Identity=30.7984790874525, Blast_Score=114, Evalue=7e-26,
Organism=Saccharomyces cerevisiae, GI6320506, Length=415, Percent_Identity=32.5301204819277, Blast_Score=178, Evalue=1e-45,
Organism=Saccharomyces cerevisiae, GI6321822, Length=414, Percent_Identity=31.6425120772947, Blast_Score=163, Evalue=3e-41,
Organism=Drosophila melanogaster, GI21357643, Length=280, Percent_Identity=31.0714285714286, Blast_Score=121, Evalue=8e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PROB_KOSOT (C5CE10)

Other databases:

- EMBL:   CP001634
- RefSeq:   YP_002941116.1
- GeneID:   7968736
- GenomeReviews:   CP001634_GR
- KEGG:   kol:Kole_1419
- ProtClustDB:   PRK05429
- GO:   GO:0005737
- HAMAP:   MF_00456
- InterPro:   IPR001048
- InterPro:   IPR001057
- InterPro:   IPR011529
- InterPro:   IPR005715
- InterPro:   IPR019797
- InterPro:   IPR002478
- InterPro:   IPR015947
- Gene3D:   G3DSA:3.40.1160.10
- PIRSF:   PIRSF000729
- PRINTS:   PR00474
- SMART:   SM00359
- TIGRFAMs:   TIGR01027

Pfam domain/function: PF00696 AA_kinase; PF01472 PUA; SSF53633 Aa_kinase; SSF88697 PUA-like

EC number: =2.7.2.11

Molecular weight: Translated: 39175; Mature: 39043

Theoretical pI: Translated: 8.29; Mature: 8.29

Prosite motif: PS00902 GLUTAMATE_5_KINASE; PS50890 PUA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKITIKVGSNLLVQQDGQLDKRYIVELCREIGNLMSEGHQVVLVSSGARAAGYGYLNKS
CEEEEEEECCCEEEECCCCCCHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCC
NAQEADLYMKQALCAVGQVQLMKLYESAMSFYGIKVAQILLTREDFSHRKRFLNLRNTLI
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GLTEMGILPIVNENDSVATEEIMFGDNDVLASMFAIGWNADYLLLMTSVDGVIDQNGKVI
HHHHCCEEEEECCCCCCEEHEEEECCCHHHHHHHHHCCCCCEEEEEEECCCEECCCCCEE
PFYREDTTNMAIAKNASSRWGSGGITSKIRAARAAAAAGIQTSICNGKKLENIAQFVLTG
EEEECCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHCCCHHCCCCCHHHHHHHHHEEEC
QTGTVFERVGPIKAKKAWIGFLSKPKGSIFINEGAKIAIENNRSLLPVGVVHIEGDFQAG
CCCHHHHHCCCCHHHHHHHHHHCCCCCCEEEECCCEEEEECCCCEEEEEEEEEECCCCCC
DVVEIRLDDGTFLGKGIINFSSAEAKKIVGLKSDQLEDVLGYSCSKVLIHIDNLWKRDN
CEEEEEECCCCEEECCEEECCCCCHHHEECCCCHHHHHHHCCCHHEEEEEEHHHHCCCC
>Mature Secondary Structure 
AKITIKVGSNLLVQQDGQLDKRYIVELCREIGNLMSEGHQVVLVSSGARAAGYGYLNKS
EEEEEEECCCEEEECCCCCCHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCC
NAQEADLYMKQALCAVGQVQLMKLYESAMSFYGIKVAQILLTREDFSHRKRFLNLRNTLI
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GLTEMGILPIVNENDSVATEEIMFGDNDVLASMFAIGWNADYLLLMTSVDGVIDQNGKVI
HHHHCCEEEEECCCCCCEEHEEEECCCHHHHHHHHHCCCCCEEEEEEECCCEECCCCCEE
PFYREDTTNMAIAKNASSRWGSGGITSKIRAARAAAAAGIQTSICNGKKLENIAQFVLTG
EEEECCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHCCCHHCCCCCHHHHHHHHHEEEC
QTGTVFERVGPIKAKKAWIGFLSKPKGSIFINEGAKIAIENNRSLLPVGVVHIEGDFQAG
CCCHHHHHCCCCHHHHHHHHHHCCCCCCEEEECCCEEEEECCCCEEEEEEEEEECCCCCC
DVVEIRLDDGTFLGKGIINFSSAEAKKIVGLKSDQLEDVLGYSCSKVLIHIDNLWKRDN
CEEEEEECCCCEEECCEEECCCCCHHHEECCCCHHHHHHHCCCHHEEEEEEHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA