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The map label for this gene is proA

Identifier: 239617793

GI number: 239617793

Start: 1511078

End: 1512322

Strand: Direct

Name: proA

Synonym: Kole_1418

Alternate gene names: 239617793

Gene position: 1511078-1512322 (Clockwise)

Preceding gene: 239617792

Following gene: 239617794

Centisome position: 65.64

GC content: 44.9

Gene sequence:

>1245_bases
ATGGAAGAATTAATCCAAAAAGCACGTTCGGTGAAAAAAGCCAGCATAAAACTTCAATCCGTTTCAACCGTCCAGAAGAA
TGAAGCGTTACTTCAGATTGGGGCTGCGCTCAAAAGAAAGGAAGATGCCATTCTTAAAGCCAACAAAATGGACGTAAAGG
AAGCACGAGATAAGGGAATCAGAGATTCCTTAATTGACCGTCTCGCTTTGAATCAAAAACGAATCGATGACATGATTCAA
TCATGTCACCGTGTTGCAGGTCTTAAAGATCCCGTAGGTGAGGTTATCGCTTCCTGGGTGCAAAGTGATGGTTTACGTAT
AAGTAAGGTCAGGGTTCCCATCGGGGCGATTGGAATAATATATGAATCCAGGCCAAATGTTACCGTAGAAGCTTCTATCC
TGGCCCTAAAAACAGGTAATTCAATCCTTTTGCGTGGTGGCTCGGACGCATTTCACTCCAATATGGCTATCGTAAAAGCG
ATCAAAGAGGGACTTTCTCGCAGTTCTCTTCCCGTGGAATCAATTGAAATTGTTGAAAGCACAGACAGAAAACTGGTAGA
ACAGATGTTGAAACTTCGGGAGTATCTTTCGCTTATCGTTCCCCGCGGTAGTAACAGGTTGATTCAATACGTCGTTGAAA
ATTCCTCCATACCGGTACTTGAAACGGGTGTTGGTAACTGCCACATATTCGTAGACGATTCTGCGGATCTTGAAAAAGCC
TTAGAAATTGTGGACAATGCAAAAACCCAGCGCCCGGGAACTTGCAATGCTGTTGAGACACTTCTGGTGCACCAAAGTAT
TGCAGCAGCCTTCCTCCCTATGATGGCAGATCGCCTTTCAAAGAAAGGTGTCGAAATTAGGGGATGTAGCCAGACACAGA
AATTTATAAAAGTTAAACCCGCAACCGATTCTGATTGGGAAACCGAATTTCTCGACCTCATTTTGGCCGTTCGCGTGGTC
AATTCACTGGATGAAGCGATAGCCCATATACAGAAATATTCAAGCGGTCACTCTGAGGCGATCCTTACGGAGAACTATTT
CAACGCAAAAAAATTCGTTGAGAATATCGATGCCGCTGCGGTTTACGTGAACGCATCCACCAGATTCACTGACGGTGGGC
AGTTTGGCTTCGGCGGTGAAATCGGAATCAGTACGCAGAGACTCCACGCAAGAGGGCCTGTAGGACTCGAAGGCTTGACC
ACTTATAAATACGTGATTCTTGGTGATTACAACGTGAGGAGATAA

Upstream 100 bases:

>100_bases
GCAATTTCATAAAAAAACTGTAAAATTCAAAAAATTCCAAGATCTCGTATTGATTAAACAGTTGATAAATCCCGAGAATG
GGATAAAGGGGGATGAAAAA

Downstream 100 bases:

>100_bases
TTATGGCAAAGATAACCATAAAAGTTGGTAGCAACCTGTTAGTGCAACAAGACGGTCAGCTAGATAAACGATATATAGTA
GAACTCTGTCGTGAAATCGG

Product: gamma-glutamyl phosphate reductase

Products: NA

Alternate protein names: GPR; Glutamate-5-semialdehyde dehydrogenase; Glutamyl-gamma-semialdehyde dehydrogenase; GSA dehydrogenase

Number of amino acids: Translated: 414; Mature: 414

Protein sequence:

>414_residues
MEELIQKARSVKKASIKLQSVSTVQKNEALLQIGAALKRKEDAILKANKMDVKEARDKGIRDSLIDRLALNQKRIDDMIQ
SCHRVAGLKDPVGEVIASWVQSDGLRISKVRVPIGAIGIIYESRPNVTVEASILALKTGNSILLRGGSDAFHSNMAIVKA
IKEGLSRSSLPVESIEIVESTDRKLVEQMLKLREYLSLIVPRGSNRLIQYVVENSSIPVLETGVGNCHIFVDDSADLEKA
LEIVDNAKTQRPGTCNAVETLLVHQSIAAAFLPMMADRLSKKGVEIRGCSQTQKFIKVKPATDSDWETEFLDLILAVRVV
NSLDEAIAHIQKYSSGHSEAILTENYFNAKKFVENIDAAAVYVNASTRFTDGGQFGFGGEIGISTQRLHARGPVGLEGLT
TYKYVILGDYNVRR

Sequences:

>Translated_414_residues
MEELIQKARSVKKASIKLQSVSTVQKNEALLQIGAALKRKEDAILKANKMDVKEARDKGIRDSLIDRLALNQKRIDDMIQ
SCHRVAGLKDPVGEVIASWVQSDGLRISKVRVPIGAIGIIYESRPNVTVEASILALKTGNSILLRGGSDAFHSNMAIVKA
IKEGLSRSSLPVESIEIVESTDRKLVEQMLKLREYLSLIVPRGSNRLIQYVVENSSIPVLETGVGNCHIFVDDSADLEKA
LEIVDNAKTQRPGTCNAVETLLVHQSIAAAFLPMMADRLSKKGVEIRGCSQTQKFIKVKPATDSDWETEFLDLILAVRVV
NSLDEAIAHIQKYSSGHSEAILTENYFNAKKFVENIDAAAVYVNASTRFTDGGQFGFGGEIGISTQRLHARGPVGLEGLT
TYKYVILGDYNVRR
>Mature_414_residues
MEELIQKARSVKKASIKLQSVSTVQKNEALLQIGAALKRKEDAILKANKMDVKEARDKGIRDSLIDRLALNQKRIDDMIQ
SCHRVAGLKDPVGEVIASWVQSDGLRISKVRVPIGAIGIIYESRPNVTVEASILALKTGNSILLRGGSDAFHSNMAIVKA
IKEGLSRSSLPVESIEIVESTDRKLVEQMLKLREYLSLIVPRGSNRLIQYVVENSSIPVLETGVGNCHIFVDDSADLEKA
LEIVDNAKTQRPGTCNAVETLLVHQSIAAAFLPMMADRLSKKGVEIRGCSQTQKFIKVKPATDSDWETEFLDLILAVRVV
NSLDEAIAHIQKYSSGHSEAILTENYFNAKKFVENIDAAAVYVNASTRFTDGGQFGFGGEIGISTQRLHARGPVGLEGLT
TYKYVILGDYNVRR

Specific function: Catalyzes the NADPH dependent reduction of L-gamma- glutamyl 5-phosphate into L-glutamate 5-semialdehyde and phosphate. The product spontaneously undergoes cyclization to form 1-pyrroline-5-carboxylate

COG id: COG0014

COG function: function code E; Gamma-glutamyl phosphate reductase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gamma-glutamyl phosphate reductase family

Homologues:

Organism=Homo sapiens, GI21361368, Length=410, Percent_Identity=34.8780487804878, Blast_Score=258, Evalue=6e-69,
Organism=Homo sapiens, GI62912457, Length=410, Percent_Identity=34.8780487804878, Blast_Score=258, Evalue=7e-69,
Organism=Escherichia coli, GI1786438, Length=415, Percent_Identity=45.3012048192771, Blast_Score=395, Evalue=1e-111,
Organism=Caenorhabditis elegans, GI17569969, Length=413, Percent_Identity=35.5932203389831, Blast_Score=275, Evalue=4e-74,
Organism=Caenorhabditis elegans, GI17569967, Length=413, Percent_Identity=35.5932203389831, Blast_Score=275, Evalue=4e-74,
Organism=Saccharomyces cerevisiae, GI6324899, Length=429, Percent_Identity=38.4615384615385, Blast_Score=290, Evalue=2e-79,
Organism=Drosophila melanogaster, GI21357643, Length=404, Percent_Identity=37.1287128712871, Blast_Score=274, Evalue=6e-74,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PROA_KOSOT (C5CE09)

Other databases:

- EMBL:   CP001634
- RefSeq:   YP_002941115.1
- ProteinModelPortal:   C5CE09
- GeneID:   7968735
- GenomeReviews:   CP001634_GR
- KEGG:   kol:Kole_1418
- ProtClustDB:   PRK00197
- GO:   GO:0005737
- HAMAP:   MF_00412
- InterPro:   IPR016161
- InterPro:   IPR016163
- InterPro:   IPR016162
- InterPro:   IPR015590
- InterPro:   IPR000965
- InterPro:   IPR020593
- InterPro:   IPR012134
- Gene3D:   G3DSA:3.40.309.10
- Gene3D:   G3DSA:3.40.605.10
- PANTHER:   PTHR11063:SF1
- PIRSF:   PIRSF000151
- TIGRFAMs:   TIGR00407

Pfam domain/function: PF00171 Aldedh; SSF53720 Aldehyde_DH/Histidinol_DH

EC number: =1.2.1.41

Molecular weight: Translated: 45487; Mature: 45487

Theoretical pI: Translated: 8.81; Mature: 8.81

Prosite motif: PS01223 PROA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEELIQKARSVKKASIKLQSVSTVQKNEALLQIGAALKRKEDAILKANKMDVKEARDKGI
CHHHHHHHHHHHHHHEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCH
RDSLIDRLALNQKRIDDMIQSCHRVAGLKDPVGEVIASWVQSDGLRISKVRVPIGAIGII
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCEEEEEECCEEEEEEE
YESRPNVTVEASILALKTGNSILLRGGSDAFHSNMAIVKAIKEGLSRSSLPVESIEIVES
EECCCCEEEEEEEEEEECCCEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHH
TDRKLVEQMLKLREYLSLIVPRGSNRLIQYVVENSSIPVLETGVGNCHIFVDDSADLEKA
HHHHHHHHHHHHHHHHHHHCCCCCCCEEHEEECCCCCCEEECCCCEEEEEECCCCCHHHH
LEIVDNAKTQRPGTCNAVETLLVHQSIAAAFLPMMADRLSKKGVEIRGCSQTQKFIKVKP
HHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCEEEEEC
ATDSDWETEFLDLILAVRVVNSLDEAIAHIQKYSSGHSEAILTENYFNAKKFVENIDAAA
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCHHHHHHHHHCCCCEE
VYVNASTRFTDGGQFGFGGEIGISTQRLHARGPVGLEGLTTYKYVILGDYNVRR
EEEECCCEECCCCCCCCCCCCCCCHHHEECCCCCCCCCCCEEEEEEEECCCCCC
>Mature Secondary Structure
MEELIQKARSVKKASIKLQSVSTVQKNEALLQIGAALKRKEDAILKANKMDVKEARDKGI
CHHHHHHHHHHHHHHEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCH
RDSLIDRLALNQKRIDDMIQSCHRVAGLKDPVGEVIASWVQSDGLRISKVRVPIGAIGII
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCEEEEEECCEEEEEEE
YESRPNVTVEASILALKTGNSILLRGGSDAFHSNMAIVKAIKEGLSRSSLPVESIEIVES
EECCCCEEEEEEEEEEECCCEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHH
TDRKLVEQMLKLREYLSLIVPRGSNRLIQYVVENSSIPVLETGVGNCHIFVDDSADLEKA
HHHHHHHHHHHHHHHHHHHCCCCCCCEEHEEECCCCCCEEECCCCEEEEEECCCCCHHHH
LEIVDNAKTQRPGTCNAVETLLVHQSIAAAFLPMMADRLSKKGVEIRGCSQTQKFIKVKP
HHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCEEEEEC
ATDSDWETEFLDLILAVRVVNSLDEAIAHIQKYSSGHSEAILTENYFNAKKFVENIDAAA
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCHHHHHHHHHCCCCEE
VYVNASTRFTDGGQFGFGGEIGISTQRLHARGPVGLEGLTTYKYVILGDYNVRR
EEEECCCEECCCCCCCCCCCCCCCHHHEECCCCCCCCCCCEEEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA