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The map label for this gene is murE [H]

Identifier: 239617758

GI number: 239617758

Start: 1470338

End: 1471831

Strand: Reverse

Name: murE [H]

Synonym: Kole_1383

Alternate gene names: 239617758

Gene position: 1471831-1470338 (Counterclockwise)

Preceding gene: 239617765

Following gene: 239617757

Centisome position: 63.93

GC content: 42.9

Gene sequence:

>1494_bases
GTGAAACTTTCCCATCTCATCGAGCTTCTCGGTAATAACCTCGTTGAGGTTACCAACCTCAACGGGAAAGACCCCGAGGT
CAATGATGTGGTGAGTAACTCCCGCCTTCTCAAGCCCGGAAACCTCTTTATCTGTCTTCAAGGTACTTCTTTTGACTCCC
ATTTGCTCGCTCCTGAACTTCAAGAAAGAGGTGCAGCTGCTTTAATTGCTGAGAAAAAAATCGATGTTGATCTTGATATT
CCTATTATATACGTTAAAAGCAGCAGGCTTGCAGAAGCACATTTGTACATGGAAATCAACGACCATCCCTACAAAAAACT
CACGACAATCGGAGTTACTGGAACGAACGGAAAGACCACGGTAACCACTCTGATAAACCACATTCTCGAAACCTTTCAAA
GAAAATCTGCTCTCGTGGGAACCGTAAAGAACGTGATCGGTGACTATGTCTCGACAAATCCCAAAAACACCACTCCCGGA
CCAGCTACTCTGGCGAAATACATGAAGATGGCTGTAGAGAAAAATCTAGAATACTTCGTTATGGAAGTTTCTTCTCACGG
TCTTGCAATGCACAGAGTCGATGGAATGCGCTTTGATGTTGCTGTGCTTACAAACGTAACACGTGATCACCTCGATTTTC
ACGCTTCTTTTGAAGATTATTACAGGACTAAGTTGCGGATCTTTAATTTACTCAAACCTGTCGGAAAAGCTGTGGTGAAT
TCGGATAACGTAAATATTGGAGATATAAAGCTCCCGAGGGATAAAATAATAACCTATGGCTTTGGAAACGAATCTGACTT
TCGCATGGAAAACCTGGAGATCTCCAGAACAGGTATGTATTTCACCGTTGAGACACCGTTTGGTTCCAAACATAAAGTTT
ACACGCGCCTCGTTGGCGAACACAATGCTTATAACGTAACCGCCACAATCGCTACAATGGAATCTTTGAATTACGACACC
GAACACATCCTGGAAGCAATTTCCACTTTCAGTGGTGTCCCGGGAAGATTCGAATTCGTTGAGGAAGCTACAAAATACGG
CTTCGATGTCCTTGTAGATTTTGCTCATACTCCAGACGCTCTGGAAAAACTTCTGAAAACTGCAAAAAAACTCTCAGAGG
GAAGAATCATTATTGTCTTTGGCGCCGGTGGAGACGCCGATAAAGGTAAAAGACCGTTAATGGGAGAAATCGCCTCCAGA
TTCGCTGATGTTGTTATCTTAACGTCTGATGATCCCAAAAAAGAAGATCCCGAAGAAATCCTGAATGACATAGAAGCAGG
AATAAGCAAAACAAAGCCGTACCTTGTCATTCCGGATAGAAGAGAGGCTATATCTGTAGCTCTTACGCTGGCAAATCGAC
AGGACACCGTTTTAATAGCGGGAAGAGGACATGAAGATTATCAGGCCTTTGCGGATTATGTTGTAGCTTTTAATGATAAA
AGAGTCGTTAAAGAGATTCTGGATACAAAATTTCGGAGGCATGCTAAAAGGTGA

Upstream 100 bases:

>100_bases
CCTCCCTGGAGACTGCTAATTTAATATTAGCATGTAGTATACCTGTTGTCAAGTCGAAATTGCGATGATACAATAGTTTC
AGCGAAAGGGGGGGAGGCAT

Downstream 100 bases:

>100_bases
GCTCATCTAAACAACTTGACGTGAAACGTTTTCTGAAACTTACTGACGGAAGAAAAATCAGAATCGACTCGCGAGTGGTT
AAAATCGGGGACGTATTCAT

Product: UDP-N-acetylmuramyl-tripeptide synthetase

Products: NA

Alternate protein names: D-lysine-adding enzyme; L-lysine-adding enzyme; UDP-MurNAc-L-Ala-D-Glu:LD-Lys ligase; UDP-MurNAc-tripeptide synthetase; UDP-N-acetylmuramyl-tripeptide synthetase [H]

Number of amino acids: Translated: 497; Mature: 497

Protein sequence:

>497_residues
MKLSHLIELLGNNLVEVTNLNGKDPEVNDVVSNSRLLKPGNLFICLQGTSFDSHLLAPELQERGAAALIAEKKIDVDLDI
PIIYVKSSRLAEAHLYMEINDHPYKKLTTIGVTGTNGKTTVTTLINHILETFQRKSALVGTVKNVIGDYVSTNPKNTTPG
PATLAKYMKMAVEKNLEYFVMEVSSHGLAMHRVDGMRFDVAVLTNVTRDHLDFHASFEDYYRTKLRIFNLLKPVGKAVVN
SDNVNIGDIKLPRDKIITYGFGNESDFRMENLEISRTGMYFTVETPFGSKHKVYTRLVGEHNAYNVTATIATMESLNYDT
EHILEAISTFSGVPGRFEFVEEATKYGFDVLVDFAHTPDALEKLLKTAKKLSEGRIIIVFGAGGDADKGKRPLMGEIASR
FADVVILTSDDPKKEDPEEILNDIEAGISKTKPYLVIPDRREAISVALTLANRQDTVLIAGRGHEDYQAFADYVVAFNDK
RVVKEILDTKFRRHAKR

Sequences:

>Translated_497_residues
MKLSHLIELLGNNLVEVTNLNGKDPEVNDVVSNSRLLKPGNLFICLQGTSFDSHLLAPELQERGAAALIAEKKIDVDLDI
PIIYVKSSRLAEAHLYMEINDHPYKKLTTIGVTGTNGKTTVTTLINHILETFQRKSALVGTVKNVIGDYVSTNPKNTTPG
PATLAKYMKMAVEKNLEYFVMEVSSHGLAMHRVDGMRFDVAVLTNVTRDHLDFHASFEDYYRTKLRIFNLLKPVGKAVVN
SDNVNIGDIKLPRDKIITYGFGNESDFRMENLEISRTGMYFTVETPFGSKHKVYTRLVGEHNAYNVTATIATMESLNYDT
EHILEAISTFSGVPGRFEFVEEATKYGFDVLVDFAHTPDALEKLLKTAKKLSEGRIIIVFGAGGDADKGKRPLMGEIASR
FADVVILTSDDPKKEDPEEILNDIEAGISKTKPYLVIPDRREAISVALTLANRQDTVLIAGRGHEDYQAFADYVVAFNDK
RVVKEILDTKFRRHAKR
>Mature_497_residues
MKLSHLIELLGNNLVEVTNLNGKDPEVNDVVSNSRLLKPGNLFICLQGTSFDSHLLAPELQERGAAALIAEKKIDVDLDI
PIIYVKSSRLAEAHLYMEINDHPYKKLTTIGVTGTNGKTTVTTLINHILETFQRKSALVGTVKNVIGDYVSTNPKNTTPG
PATLAKYMKMAVEKNLEYFVMEVSSHGLAMHRVDGMRFDVAVLTNVTRDHLDFHASFEDYYRTKLRIFNLLKPVGKAVVN
SDNVNIGDIKLPRDKIITYGFGNESDFRMENLEISRTGMYFTVETPFGSKHKVYTRLVGEHNAYNVTATIATMESLNYDT
EHILEAISTFSGVPGRFEFVEEATKYGFDVLVDFAHTPDALEKLLKTAKKLSEGRIIIVFGAGGDADKGKRPLMGEIASR
FADVVILTSDDPKKEDPEEILNDIEAGISKTKPYLVIPDRREAISVALTLANRQDTVLIAGRGHEDYQAFADYVVAFNDK
RVVKEILDTKFRRHAKR

Specific function: Catalyzes the addition of both L- and D-lysine to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan. Is also able to use meso-diaminopimelate as the amino acid substrate in v

COG id: COG0769

COG function: function code M; UDP-N-acetylmuramyl tripeptide synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family. MurE subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786273, Length=473, Percent_Identity=34.6723044397463, Blast_Score=228, Evalue=6e-61,
Organism=Escherichia coli, GI1786274, Length=339, Percent_Identity=28.023598820059, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1786276, Length=234, Percent_Identity=27.7777777777778, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI1786279, Length=270, Percent_Identity=27.4074074074074, Blast_Score=66, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018109
- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005761 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.7 [H]

Molecular weight: Translated: 55485; Mature: 55485

Theoretical pI: Translated: 6.69; Mature: 6.69

Prosite motif: PS01011 FOLYLPOLYGLU_SYNT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLSHLIELLGNNLVEVTNLNGKDPEVNDVVSNSRLLKPGNLFICLQGTSFDSHLLAPEL
CCHHHHHHHHCCCEEEEECCCCCCCCHHHHHCCCEEECCCCEEEEEECCCCCCCCCCHHH
QERGAAALIAEKKIDVDLDIPIIYVKSSRLAEAHLYMEINDHPYKKLTTIGVTGTNGKTT
HHCCCEEEEEECCCCEEECCEEEEEECCCCEEEEEEEEECCCCCCEEEEEEEECCCCCHH
VTTLINHILETFQRKSALVGTVKNVIGDYVSTNPKNTTPGPATLAKYMKMAVEKNLEYFV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCEEEE
MEVSSHGLAMHRVDGMRFDVAVLTNVTRDHLDFHASFEDYYRTKLRIFNLLKPVGKAVVN
EEECCCCEEEEECCCCEEEHHHHHHCHHHHHHEECCHHHHHHHHHHHHHHHHHHHHHHCC
SDNVNIGDIKLPRDKIITYGFGNESDFRMENLEISRTGMYFTVETPFGSKHKVYTRLVGE
CCCCEECCEECCHHHEEEEECCCCCCCEEECEEEEECCEEEEEECCCCCCCHHHHHHHCC
HNAYNVTATIATMESLNYDTEHILEAISTFSGVPGRFEFVEEATKYGFDVLVDFAHTPDA
CCCEEEEEEEEEHHHCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHCCCEEEEECCCCHHH
LEKLLKTAKKLSEGRIIIVFGAGGDADKGKRPLMGEIASRFADVVILTSDDPKKEDPEEI
HHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCHHHHHHHHHEEEEEEECCCCCCCCHHHH
LNDIEAGISKTKPYLVIPDRREAISVALTLANRQDTVLIAGRGHEDYQAFADYVVAFNDK
HHHHHHHHHCCCCEEEECCCCCEEEEEEEEECCCCEEEEEECCCHHHHHHHHHHEEECCH
RVVKEILDTKFRRHAKR
HHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKLSHLIELLGNNLVEVTNLNGKDPEVNDVVSNSRLLKPGNLFICLQGTSFDSHLLAPEL
CCHHHHHHHHCCCEEEEECCCCCCCCHHHHHCCCEEECCCCEEEEEECCCCCCCCCCHHH
QERGAAALIAEKKIDVDLDIPIIYVKSSRLAEAHLYMEINDHPYKKLTTIGVTGTNGKTT
HHCCCEEEEEECCCCEEECCEEEEEECCCCEEEEEEEEECCCCCCEEEEEEEECCCCCHH
VTTLINHILETFQRKSALVGTVKNVIGDYVSTNPKNTTPGPATLAKYMKMAVEKNLEYFV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCEEEE
MEVSSHGLAMHRVDGMRFDVAVLTNVTRDHLDFHASFEDYYRTKLRIFNLLKPVGKAVVN
EEECCCCEEEEECCCCEEEHHHHHHCHHHHHHEECCHHHHHHHHHHHHHHHHHHHHHHCC
SDNVNIGDIKLPRDKIITYGFGNESDFRMENLEISRTGMYFTVETPFGSKHKVYTRLVGE
CCCCEECCEECCHHHEEEEECCCCCCCEEECEEEEECCEEEEEECCCCCCCHHHHHHHCC
HNAYNVTATIATMESLNYDTEHILEAISTFSGVPGRFEFVEEATKYGFDVLVDFAHTPDA
CCCEEEEEEEEEHHHCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHCCCEEEEECCCCHHH
LEKLLKTAKKLSEGRIIIVFGAGGDADKGKRPLMGEIASRFADVVILTSDDPKKEDPEEI
HHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCHHHHHHHHHEEEEEEECCCCCCCCHHHH
LNDIEAGISKTKPYLVIPDRREAISVALTLANRQDTVLIAGRGHEDYQAFADYVVAFNDK
HHHHHHHHHCCCCEEEECCCCCEEEEEEEEECCCCEEEEEECCCHHHHHHHHHHEEECCH
RVVKEILDTKFRRHAKR
HHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10360571 [H]