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The map label for this gene is murF [H]

Identifier: 239617757

GI number: 239617757

Start: 1469001

End: 1470341

Strand: Reverse

Name: murF [H]

Synonym: Kole_1382

Alternate gene names: 239617757

Gene position: 1470341-1469001 (Counterclockwise)

Preceding gene: 239617758

Following gene: 239617756

Centisome position: 63.87

GC content: 40.87

Gene sequence:

>1341_bases
GTGAGCTCATCTAAACAACTTGACGTGAAACGTTTTCTGAAACTTACTGACGGAAGAAAAATCAGAATCGACTCGCGAGT
GGTTAAAATCGGGGACGTATTCATAGCCTTGAAGGGCACGAGGGTTGACGGAAATGATTTCATAGACGACGCTCTCAAAC
GTGGAGCAATTTTTGGCGTCTCAAACCGAAGTTCAGACAATCCTGCTGTTTTCTTTCATCCAGACCCGAAGAAACTGCTC
ATTGAAGCAGCTGCTTTTATTCTGAAGCACTCAAAAATCAAGCACAAAATCGCGATAACCGGATCTAACGGAAAAACAAC
GACCAAAGAAATTCTTGCTTCCATACTTTCTGACTATGGTAAGGTGTTCAAAACACCAGGTAACCTCAACACTGAAATAG
GTATTCCCATAGCTATTCTCGAAAACCGTTCACAGCTGGCAGAATGCGATTATGCCATCTTTGAATTCGGAACGAGCAAA
AAAGGGGATATCTCTTTTTTGACAGACCTTGTAAATCCAGATATTGCCGTTCTCTTGAACGTGGGCACGGCTCACCGGGG
GAACTTCAATGACGAAACCGAATTACTTCAGGAAAAGTGCTCGATATTCGAAACTCTTCAGAAAGATTCAACAGCAATCA
CAAACGGTGATGATAAAAGAATCAGGTCAATGATTTCGGAGCTCAGTTGCAAAAAGTTGTTTTTTGGACAGCGCAACGGA
GATTTGAAGATAATGAAATTTGACTATTCCAAAAACAATACCTTTGTCGCTTTCAAAGCTTTCGGAGAAATGAAGATGGC
AAAGCTCAAAGGGATCTGGAATTCCGGTCAATTACTGGACTTTGGTGCCGCTTATCTAACAGCTCTTTCTTTAAAAATTG
AAGAACCGCTCCTCAATATCAACAGAATTATCTTACCTTTCAACAATCGCTTCTCAGTGGAGAACATTGCGGGAATAATC
CTGATAAATGACTGTTACAACAGCAGTTTAGAATCTGTAGAGTCTGCCATAAAAAGTATAGCCAAGCTAAAAAAAGAGAG
AACTTTTGCTGTTGTAGGCTCTATACTTGAACAGGGAAAATATTCAAAAAAAACCCATGAAGAACTCGGAAGACTATTGA
ACTTTTTTGACCACATAGTTCTTTACACAAAGGACACCCAGGTTGAGGCAGTACAAAATACCGCAAACATTGATTTCAAA
AGTTCGAATCCGGAAGAAGTCGCCGAATGGCTGAAAAAAAACTGCCGCTATGGCGATCTGGTTTATTTCAAAGCATCAAG
AGGTGTTGCACTCGAAGAAGCTATCCTGAGTTTTCGTAGGAGGTTGCAGAGTGGAGAATAA

Upstream 100 bases:

>100_bases
ATGAAGATTATCAGGCCTTTGCGGATTATGTTGTAGCTTTTAATGATAAAAGAGTCGTTAAAGAGATTCTGGATACAAAA
TTTCGGAGGCATGCTAAAAG

Downstream 100 bases:

>100_bases
CGTAATTGTTTTTTTTCTTTCCTTTTTAAGTAGTGTCGTATTTATTGAGGGATTCAAACGTTTCCAGAAAAAACTTCGAA
TCGGACAATACATTCGCGAA

Product: UDP-N-acetylmuramoylalanyl-D-glutamyl-2, 6-diaminopimelate/D-alanyl-D-alanyl ligase

Products: NA

Alternate protein names: D-alanyl-D-alanine-adding enzyme; UDP-MurNAc-pentapeptide synthetase [H]

Number of amino acids: Translated: 446; Mature: 445

Protein sequence:

>446_residues
MSSSKQLDVKRFLKLTDGRKIRIDSRVVKIGDVFIALKGTRVDGNDFIDDALKRGAIFGVSNRSSDNPAVFFHPDPKKLL
IEAAAFILKHSKIKHKIAITGSNGKTTTKEILASILSDYGKVFKTPGNLNTEIGIPIAILENRSQLAECDYAIFEFGTSK
KGDISFLTDLVNPDIAVLLNVGTAHRGNFNDETELLQEKCSIFETLQKDSTAITNGDDKRIRSMISELSCKKLFFGQRNG
DLKIMKFDYSKNNTFVAFKAFGEMKMAKLKGIWNSGQLLDFGAAYLTALSLKIEEPLLNINRIILPFNNRFSVENIAGII
LINDCYNSSLESVESAIKSIAKLKKERTFAVVGSILEQGKYSKKTHEELGRLLNFFDHIVLYTKDTQVEAVQNTANIDFK
SSNPEEVAEWLKKNCRYGDLVYFKASRGVALEEAILSFRRRLQSGE

Sequences:

>Translated_446_residues
MSSSKQLDVKRFLKLTDGRKIRIDSRVVKIGDVFIALKGTRVDGNDFIDDALKRGAIFGVSNRSSDNPAVFFHPDPKKLL
IEAAAFILKHSKIKHKIAITGSNGKTTTKEILASILSDYGKVFKTPGNLNTEIGIPIAILENRSQLAECDYAIFEFGTSK
KGDISFLTDLVNPDIAVLLNVGTAHRGNFNDETELLQEKCSIFETLQKDSTAITNGDDKRIRSMISELSCKKLFFGQRNG
DLKIMKFDYSKNNTFVAFKAFGEMKMAKLKGIWNSGQLLDFGAAYLTALSLKIEEPLLNINRIILPFNNRFSVENIAGII
LINDCYNSSLESVESAIKSIAKLKKERTFAVVGSILEQGKYSKKTHEELGRLLNFFDHIVLYTKDTQVEAVQNTANIDFK
SSNPEEVAEWLKKNCRYGDLVYFKASRGVALEEAILSFRRRLQSGE
>Mature_445_residues
SSSKQLDVKRFLKLTDGRKIRIDSRVVKIGDVFIALKGTRVDGNDFIDDALKRGAIFGVSNRSSDNPAVFFHPDPKKLLI
EAAAFILKHSKIKHKIAITGSNGKTTTKEILASILSDYGKVFKTPGNLNTEIGIPIAILENRSQLAECDYAIFEFGTSKK
GDISFLTDLVNPDIAVLLNVGTAHRGNFNDETELLQEKCSIFETLQKDSTAITNGDDKRIRSMISELSCKKLFFGQRNGD
LKIMKFDYSKNNTFVAFKAFGEMKMAKLKGIWNSGQLLDFGAAYLTALSLKIEEPLLNINRIILPFNNRFSVENIAGIIL
INDCYNSSLESVESAIKSIAKLKKERTFAVVGSILEQGKYSKKTHEELGRLLNFFDHIVLYTKDTQVEAVQNTANIDFKS
SNPEEVAEWLKKNCRYGDLVYFKASRGVALEEAILSFRRRLQSGE

Specific function: Involved in cell wall formation. Catalyzes the final step in the synthesis of UDP-N-acetylmuramoyl-pentapeptide, the precursor of murein [H]

COG id: COG0770

COG function: function code M; UDP-N-acetylmuramyl pentapeptide synthase

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1786274, Length=358, Percent_Identity=27.9329608938547, Blast_Score=116, Evalue=3e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005863 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.10 [H]

Molecular weight: Translated: 49897; Mature: 49766

Theoretical pI: Translated: 9.57; Mature: 9.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSSKQLDVKRFLKLTDGRKIRIDSRVVKIGDVFIALKGTRVDGNDFIDDALKRGAIFGV
CCCCCCCCHHHHHHHCCCCEEEECCEEEEEEEEEEEEECCCCCCHHHHHHHHHCCEEEEE
SNRSSDNPAVFFHPDPKKLLIEAAAFILKHSKIKHKIAITGSNGKTTTKEILASILSDYG
CCCCCCCCEEEECCCHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCHHHHHHHHHHHHHH
KVFKTPGNLNTEIGIPIAILENRSQLAECDYAIFEFGTSKKGDISFLTDLVNPDIAVLLN
HHCCCCCCCCCCCCCEEEEECCCHHHHHCCCCEEECCCCCCCCHHHHHHHCCCCEEEEEE
VGTAHRGNFNDETELLQEKCSIFETLQKDSTAITNGDDKRIRSMISELSCKKLFFGQRNG
CCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEECCCCHHHHHHHHHHHHHHHHHCCCCCC
DLKIMKFDYSKNNTFVAFKAFGEMKMAKLKGIWNSGQLLDFGAAYLTALSLKIEEPLLNI
CEEEEEEEECCCCEEEEEEECCCHHHHHHHCCCCCCCEEHHHHHHHHHHHHEECCCCCCC
NRIILPFNNRFSVENIAGIILINDCYNSSLESVESAIKSIAKLKKERTFAVVGSILEQGK
CEEEEECCCCCCHHHCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
YSKKTHEELGRLLNFFDHIVLYTKDTQVEAVQNTANIDFKSSNPEEVAEWLKKNCRYGDL
CCHHHHHHHHHHHHHHHHEEEEECCCHHHHHHCCCCEEECCCCHHHHHHHHHHCCCCCCE
VYFKASRGVALEEAILSFRRRLQSGE
EEEECCCCCHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SSSKQLDVKRFLKLTDGRKIRIDSRVVKIGDVFIALKGTRVDGNDFIDDALKRGAIFGV
CCCCCCCHHHHHHHCCCCEEEECCEEEEEEEEEEEEECCCCCCHHHHHHHHHCCEEEEE
SNRSSDNPAVFFHPDPKKLLIEAAAFILKHSKIKHKIAITGSNGKTTTKEILASILSDYG
CCCCCCCCEEEECCCHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCHHHHHHHHHHHHHH
KVFKTPGNLNTEIGIPIAILENRSQLAECDYAIFEFGTSKKGDISFLTDLVNPDIAVLLN
HHCCCCCCCCCCCCCEEEEECCCHHHHHCCCCEEECCCCCCCCHHHHHHHCCCCEEEEEE
VGTAHRGNFNDETELLQEKCSIFETLQKDSTAITNGDDKRIRSMISELSCKKLFFGQRNG
CCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEECCCCHHHHHHHHHHHHHHHHHCCCCCC
DLKIMKFDYSKNNTFVAFKAFGEMKMAKLKGIWNSGQLLDFGAAYLTALSLKIEEPLLNI
CEEEEEEEECCCCEEEEEEECCCHHHHHHHCCCCCCCEEHHHHHHHHHHHHEECCCCCCC
NRIILPFNNRFSVENIAGIILINDCYNSSLESVESAIKSIAKLKKERTFAVVGSILEQGK
CEEEEECCCCCCHHHCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
YSKKTHEELGRLLNFFDHIVLYTKDTQVEAVQNTANIDFKSSNPEEVAEWLKKNCRYGDL
CCHHHHHHHHHHHHHHHHEEEEECCCHHHHHHCCCCEEECCCCHHHHHHHHHHCCCCCCE
VYFKASRGVALEEAILSFRRRLQSGE
EEEECCCCCHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9177243; 12060705 [H]