Click here to switch to the map view.

The map label for this gene is leuC1 [H]

Identifier: 239617608

GI number: 239617608

Start: 1305219

End: 1306472

Strand: Reverse

Name: leuC1 [H]

Synonym: Kole_1229

Alternate gene names: 239617608

Gene position: 1306472-1305219 (Counterclockwise)

Preceding gene: 239617609

Following gene: 239617607

Centisome position: 56.75

GC content: 42.98

Gene sequence:

>1254_bases
ATGGGGAAAACCATCGCCGAAAAAATAATATCCGAACATGTTGGAAGAGACGTGAATTCTGGAGATATCGTTGTTGCTAA
GGTAGATATAGCCATGGTTCAAGATGGAACCGGTCCTCTGGCTGTTGATGAGTTCTGCACAATGGGGTTTGACGTTCTTA
AAGCCCCGAATTCCATTCTTTTTATAGACCATGCCTCACCGAGTCCGCGAAAAGAATTGTCAAACGCACAAAAGAAATTG
AGAGATTTTTCGAAAAAGACAGGAGCCATTCTTTCGGATATAGGTGAGGGAGTATCGCACCAAATACTTGTTGAGAAGTA
TGTGAAGCCTGGTGACCTTGTTGTTGGTGCCGATTCTCATACATGCACTTCTGGTGCTCTTGGAGCGTTTGCTACCGGGA
TGGGATCAACAGATGTTGCACTCGCTTTCGGACTAGGGAAAGTATGGCTAAAAGTCCCGGCGAGCTTGAAATTTGTTTTG
AATGGAAAAATTCCTGATGGAGTTTTTTCAAAGGATATAATTTTACACATAATTGGTCTCATTGGAGCTGACGGTGCGAA
TTACAAAGTAATGGAATTTGAGGGAGAGGTTATAGAGCAGATCTCACAGGAAGCAAGATTCACCATAACCAATATGGCAA
TTGAAGCAGGAGCGAAATCAGGAATAATAGCCCCGGATGAGAAAACAAGAGCATTCCTAAGAGCTTATGGTAGGGAATCA
GATTACAGAGAAATAAAATCTGATCCTGATGCGGTTTACGAAAAAATCTATGAAATAGATGTATCCAATCTTGAACCGGC
GGTTGCACTTCCTCACACTGTCGATAACACTAAGAGTATCAGCGAAGTTGAAGGTATCAAGATAGATCAGGTATTCATAG
GTACCTGTACAAATGGAAGACTCGAAGATTTGAGGGTGGCAGCTTCGATTTTAAAGGGCAGAGAAAAGCATCCTGATGTG
AGGTTAATAGTTATACCTGCATCAAAAGAGGTCTATCTAAAAGCCTTAGAAGAAGGTCTGATTAAGATCTTTGTGGAAAG
CGGCGCAACAGTGCTTGCACCAGGATGCGGACCCTGTGTGGGGGTTCATCAGGGGGTTCTTGGGGATGGAGAAAGGGTTC
TATCGACCCAAAATAGAAATTTCAAAGGAAGAATGGGAAATCCAAAAGCTGAAATATTTCTTGCTTCTCCAGCAACCGCT
GCAGCTTCAGCTATAACCGGCAAAATCACCGACCCAAGGAAGTTCCTTGCATAA

Upstream 100 bases:

>100_bases
TATCGAATATACCAAATCAAATAAGATAATAAGACCGTGCGGCGTCTATGTTGGACCTATGAACGCTGAATACATACCTT
TGGAAGCGAGGGAGTGAAAA

Downstream 100 bases:

>100_bases
GGGGGAGATACAATTGATTATTAAAGGGAAAGTGTGGAAATTCGGTGATGATATTTCAACAGATCATATAGCGCCTGGAA
GATATTTTCATTTACGCACC

Product: 3-isopropylmalate dehydratase large subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase 1; IPMI 1; Isopropylmalate isomerase 1 [H]

Number of amino acids: Translated: 417; Mature: 416

Protein sequence:

>417_residues
MGKTIAEKIISEHVGRDVNSGDIVVAKVDIAMVQDGTGPLAVDEFCTMGFDVLKAPNSILFIDHASPSPRKELSNAQKKL
RDFSKKTGAILSDIGEGVSHQILVEKYVKPGDLVVGADSHTCTSGALGAFATGMGSTDVALAFGLGKVWLKVPASLKFVL
NGKIPDGVFSKDIILHIIGLIGADGANYKVMEFEGEVIEQISQEARFTITNMAIEAGAKSGIIAPDEKTRAFLRAYGRES
DYREIKSDPDAVYEKIYEIDVSNLEPAVALPHTVDNTKSISEVEGIKIDQVFIGTCTNGRLEDLRVAASILKGREKHPDV
RLIVIPASKEVYLKALEEGLIKIFVESGATVLAPGCGPCVGVHQGVLGDGERVLSTQNRNFKGRMGNPKAEIFLASPATA
AASAITGKITDPRKFLA

Sequences:

>Translated_417_residues
MGKTIAEKIISEHVGRDVNSGDIVVAKVDIAMVQDGTGPLAVDEFCTMGFDVLKAPNSILFIDHASPSPRKELSNAQKKL
RDFSKKTGAILSDIGEGVSHQILVEKYVKPGDLVVGADSHTCTSGALGAFATGMGSTDVALAFGLGKVWLKVPASLKFVL
NGKIPDGVFSKDIILHIIGLIGADGANYKVMEFEGEVIEQISQEARFTITNMAIEAGAKSGIIAPDEKTRAFLRAYGRES
DYREIKSDPDAVYEKIYEIDVSNLEPAVALPHTVDNTKSISEVEGIKIDQVFIGTCTNGRLEDLRVAASILKGREKHPDV
RLIVIPASKEVYLKALEEGLIKIFVESGATVLAPGCGPCVGVHQGVLGDGERVLSTQNRNFKGRMGNPKAEIFLASPATA
AASAITGKITDPRKFLA
>Mature_416_residues
GKTIAEKIISEHVGRDVNSGDIVVAKVDIAMVQDGTGPLAVDEFCTMGFDVLKAPNSILFIDHASPSPRKELSNAQKKLR
DFSKKTGAILSDIGEGVSHQILVEKYVKPGDLVVGADSHTCTSGALGAFATGMGSTDVALAFGLGKVWLKVPASLKFVLN
GKIPDGVFSKDIILHIIGLIGADGANYKVMEFEGEVIEQISQEARFTITNMAIEAGAKSGIIAPDEKTRAFLRAYGRESD
YREIKSDPDAVYEKIYEIDVSNLEPAVALPHTVDNTKSISEVEGIKIDQVFIGTCTNGRLEDLRVAASILKGREKHPDVR
LIVIPASKEVYLKALEEGLIKIFVESGATVLAPGCGPCVGVHQGVLGDGERVLSTQNRNFKGRMGNPKAEIFLASPATAA
ASAITGKITDPRKFLA

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate [H]

COG id: COG0065

COG function: function code E; 3-isopropylmalate dehydratase large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family. LeuC type 2 subfamily [H]

Homologues:

Organism=Homo sapiens, GI4501867, Length=444, Percent_Identity=29.2792792792793, Blast_Score=159, Evalue=6e-39,
Organism=Homo sapiens, GI8659555, Length=399, Percent_Identity=25.8145363408521, Blast_Score=86, Evalue=5e-17,
Organism=Escherichia coli, GI1786259, Length=466, Percent_Identity=33.2618025751073, Blast_Score=215, Evalue=3e-57,
Organism=Escherichia coli, GI87081781, Length=358, Percent_Identity=27.3743016759777, Blast_Score=124, Evalue=9e-30,
Organism=Escherichia coli, GI2367097, Length=458, Percent_Identity=27.9475982532751, Blast_Score=122, Evalue=3e-29,
Organism=Escherichia coli, GI1787531, Length=392, Percent_Identity=24.4897959183673, Blast_Score=85, Evalue=9e-18,
Organism=Caenorhabditis elegans, GI25149337, Length=443, Percent_Identity=31.3769751693002, Blast_Score=162, Evalue=3e-40,
Organism=Caenorhabditis elegans, GI32564738, Length=409, Percent_Identity=32.0293398533007, Blast_Score=157, Evalue=8e-39,
Organism=Caenorhabditis elegans, GI25149342, Length=284, Percent_Identity=33.0985915492958, Blast_Score=127, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI17568399, Length=400, Percent_Identity=25.5, Blast_Score=78, Evalue=7e-15,
Organism=Saccharomyces cerevisiae, GI6321429, Length=475, Percent_Identity=33.6842105263158, Blast_Score=216, Evalue=4e-57,
Organism=Saccharomyces cerevisiae, GI6320440, Length=451, Percent_Identity=33.2594235033259, Blast_Score=210, Evalue=3e-55,
Organism=Saccharomyces cerevisiae, GI6323335, Length=445, Percent_Identity=31.9101123595506, Blast_Score=193, Evalue=4e-50,
Organism=Saccharomyces cerevisiae, GI6322261, Length=453, Percent_Identity=30.242825607064, Blast_Score=183, Evalue=4e-47,
Organism=Drosophila melanogaster, GI28571643, Length=443, Percent_Identity=31.1512415349887, Blast_Score=169, Evalue=3e-42,
Organism=Drosophila melanogaster, GI281365315, Length=445, Percent_Identity=30.1123595505618, Blast_Score=163, Evalue=2e-40,
Organism=Drosophila melanogaster, GI17864292, Length=445, Percent_Identity=30.1123595505618, Blast_Score=163, Evalue=2e-40,
Organism=Drosophila melanogaster, GI161076999, Length=410, Percent_Identity=30.4878048780488, Blast_Score=157, Evalue=2e-38,
Organism=Drosophila melanogaster, GI17137564, Length=489, Percent_Identity=24.5398773006135, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24645686, Length=494, Percent_Identity=25.5060728744939, Blast_Score=73, Evalue=3e-13,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015932
- InterPro:   IPR018136
- InterPro:   IPR011826
- InterPro:   IPR015936
- InterPro:   IPR006251 [H]

Pfam domain/function: PF00330 Aconitase [H]

EC number: =4.2.1.33 [H]

Molecular weight: Translated: 44474; Mature: 44342

Theoretical pI: Translated: 6.13; Mature: 6.13

Prosite motif: PS00450 ACONITASE_1 ; PS01244 ACONITASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKTIAEKIISEHVGRDVNSGDIVVAKVDIAMVQDGTGPLAVDEFCTMGFDVLKAPNSIL
CCHHHHHHHHHHHHCCCCCCCCEEEEEEEEEEEECCCCCCHHHHHHHCCHHHHCCCCCEE
FIDHASPSPRKELSNAQKKLRDFSKKTGAILSDIGEGVSHQILVEKYVKPGDLVVGADSH
EEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCEEEECCCC
TCTSGALGAFATGMGSTDVALAFGLGKVWLKVPASLKFVLNGKIPDGVFSKDIILHIIGL
CCCCCCHHHHHCCCCCCHHHHHHHHHHEEEECCCEEEEEEECCCCCCCCHHHHHHHHHHH
IGADGANYKVMEFEGEVIEQISQEARFTITNMAIEAGAKSGIIAPDEKTRAFLRAYGRES
HCCCCCCEEEEEECCHHHHHHHHHHCEEEEHHEEECCCCCCCCCCCHHHHHHHHHHCCCC
DYREIKSDPDAVYEKIYEIDVSNLEPAVALPHTVDNTKSISEVEGIKIDQVFIGTCTNGR
HHHHHCCCHHHHHHHHHHCCCCCCCCCEECCCCCCCCCCHHHHCCEEEEEEEEEECCCCC
LEDLRVAASILKGREKHPDVRLIVIPASKEVYLKALEEGLIKIFVESGATVLAPGCGPCV
HHHHHHHHHHHCCCCCCCCEEEEEEECCHHHHHHHHHHHHEEEEEECCCEEEECCCCCHH
GVHQGVLGDGERVLSTQNRNFKGRMGNPKAEIFLASPATAAASAITGKITDPRKFLA
HHCCCCCCCCHHHHHCCCCCCCCCCCCCCEEEEEECCCHHHHHHHCCCCCCCHHHCC
>Mature Secondary Structure 
GKTIAEKIISEHVGRDVNSGDIVVAKVDIAMVQDGTGPLAVDEFCTMGFDVLKAPNSIL
CHHHHHHHHHHHHCCCCCCCCEEEEEEEEEEEECCCCCCHHHHHHHCCHHHHCCCCCEE
FIDHASPSPRKELSNAQKKLRDFSKKTGAILSDIGEGVSHQILVEKYVKPGDLVVGADSH
EEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCEEEECCCC
TCTSGALGAFATGMGSTDVALAFGLGKVWLKVPASLKFVLNGKIPDGVFSKDIILHIIGL
CCCCCCHHHHHCCCCCCHHHHHHHHHHEEEECCCEEEEEEECCCCCCCCHHHHHHHHHHH
IGADGANYKVMEFEGEVIEQISQEARFTITNMAIEAGAKSGIIAPDEKTRAFLRAYGRES
HCCCCCCEEEEEECCHHHHHHHHHHCEEEEHHEEECCCCCCCCCCCHHHHHHHHHHCCCC
DYREIKSDPDAVYEKIYEIDVSNLEPAVALPHTVDNTKSISEVEGIKIDQVFIGTCTNGR
HHHHHCCCHHHHHHHHHHCCCCCCCCCEECCCCCCCCCCHHHHCCEEEEEEEEEECCCCC
LEDLRVAASILKGREKHPDVRLIVIPASKEVYLKALEEGLIKIFVESGATVLAPGCGPCV
HHHHHHHHHHHCCCCCCCCEEEEEEECCHHHHHHHHHHHHEEEEEECCCEEEECCCCCHH
GVHQGVLGDGERVLSTQNRNFKGRMGNPKAEIFLASPATAAASAITGKITDPRKFLA
HHCCCCCCCCHHHHHCCCCCCCCCCCCCCEEEEEECCCHHHHHHHCCCCCCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10360571 [H]