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The map label for this gene is leuD [H]

Identifier: 239617607

GI number: 239617607

Start: 1304702

End: 1305205

Strand: Reverse

Name: leuD [H]

Synonym: Kole_1228

Alternate gene names: 239617607

Gene position: 1305205-1304702 (Counterclockwise)

Preceding gene: 239617608

Following gene: 239617606

Centisome position: 56.7

GC content: 37.3

Gene sequence:

>504_bases
TTGATTATTAAAGGGAAAGTGTGGAAATTCGGTGATGATATTTCAACAGATCATATAGCGCCTGGAAGATATTTTCATTT
ACGCACCAATCTTCCAGAACTCGCAAAACATGTACTTGAAGATGCGAGAGAAGATTTTCCAAAAATAGTAAAAAAAGGGG
ACATTATAGTCGCTGGAAAAAACTTTGGAATGGGATCTTCCAGGGAACATGCGCCCAGGATAATAAAACTTGCCGGAATT
TCGTGCATAATAGCGAAATCCTTTGCCAGGATATTTTACAGGAACTCTATAAATATAGGACTTCCTTTGGTAGAAATTGA
GGAAACCGATGAAATAGACGAAGGCGATGTAATAGAGGTCGATCTTAGAAATGGAATCGTCAAGAACCTCACCAAAGGGA
AAACTTACAGATTTAACCCCATGCCAGATTTTATATTGAAGATCGTAGAAAAAGGTGGAATAAGAAATTACATAAAAGAG
TTTGGAGACTACTTTGTGGAGTGA

Upstream 100 bases:

>100_bases
GCTGAAATATTTCTTGCTTCTCCAGCAACCGCTGCAGCTTCAGCTATAACCGGCAAAATCACCGACCCAAGGAAGTTCCT
TGCATAAGGGGGAGATACAA

Downstream 100 bases:

>100_bases
TTCGCCTTGGAAGGACAGAAAATAAAGGTAGAAAACAACAGTATTTTGGTTCCAAATAATCCCATAATCCCATATATAGC
AGGTGATGGAATAGGGCCCG

Product: 3-isopropylmalate dehydratase small subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase 1; IPMI 1; Isopropylmalate isomerase 1 [H]

Number of amino acids: Translated: 167; Mature: 167

Protein sequence:

>167_residues
MIIKGKVWKFGDDISTDHIAPGRYFHLRTNLPELAKHVLEDAREDFPKIVKKGDIIVAGKNFGMGSSREHAPRIIKLAGI
SCIIAKSFARIFYRNSINIGLPLVEIEETDEIDEGDVIEVDLRNGIVKNLTKGKTYRFNPMPDFILKIVEKGGIRNYIKE
FGDYFVE

Sequences:

>Translated_167_residues
MIIKGKVWKFGDDISTDHIAPGRYFHLRTNLPELAKHVLEDAREDFPKIVKKGDIIVAGKNFGMGSSREHAPRIIKLAGI
SCIIAKSFARIFYRNSINIGLPLVEIEETDEIDEGDVIEVDLRNGIVKNLTKGKTYRFNPMPDFILKIVEKGGIRNYIKE
FGDYFVE
>Mature_167_residues
MIIKGKVWKFGDDISTDHIAPGRYFHLRTNLPELAKHVLEDAREDFPKIVKKGDIIVAGKNFGMGSSREHAPRIIKLAGI
SCIIAKSFARIFYRNSINIGLPLVEIEETDEIDEGDVIEVDLRNGIVKNLTKGKTYRFNPMPDFILKIVEKGGIRNYIKE
FGDYFVE

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate [H]

COG id: COG0066

COG function: function code E; 3-isopropylmalate dehydratase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leuD family. LeuD type 2 subfamily [H]

Homologues:

Organism=Saccharomyces cerevisiae, GI6320440, Length=98, Percent_Identity=41.8367346938776, Blast_Score=75, Evalue=6e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012305
- InterPro:   IPR015937
- InterPro:   IPR015928
- InterPro:   IPR000573
- InterPro:   IPR011827 [H]

Pfam domain/function: PF00694 Aconitase_C [H]

EC number: =4.2.1.33 [H]

Molecular weight: Translated: 18980; Mature: 18980

Theoretical pI: Translated: 8.44; Mature: 8.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIIKGKVWKFGDDISTDHIAPGRYFHLRTNLPELAKHVLEDAREDFPKIVKKGDIIVAGK
CEECCCCEECCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEC
NFGMGSSREHAPRIIKLAGISCIIAKSFARIFYRNSINIGLPLVEIEETDEIDEGDVIEV
CCCCCCCCCCCCEEEEHHCHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCCEEEE
DLRNGIVKNLTKGKTYRFNPMPDFILKIVEKGGIRNYIKEFGDYFVE
ECCCCHHHHHCCCCCEEECCCHHHHHHHHHCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MIIKGKVWKFGDDISTDHIAPGRYFHLRTNLPELAKHVLEDAREDFPKIVKKGDIIVAGK
CEECCCCEECCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEC
NFGMGSSREHAPRIIKLAGISCIIAKSFARIFYRNSINIGLPLVEIEETDEIDEGDVIEV
CCCCCCCCCCCCEEEEHHCHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCCEEEE
DLRNGIVKNLTKGKTYRFNPMPDFILKIVEKGGIRNYIKEFGDYFVE
ECCCCHHHHHCCCCCEEECCCHHHHHHHHHCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10360571 [H]