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The map label for this gene is iolD [H]

Identifier: 239617379

GI number: 239617379

Start: 1044174

End: 1046045

Strand: Reverse

Name: iolD [H]

Synonym: Kole_0989

Alternate gene names: 239617379

Gene position: 1046045-1044174 (Counterclockwise)

Preceding gene: 239617380

Following gene: 239617378

Centisome position: 45.44

GC content: 44.5

Gene sequence:

>1872_bases
TTGAAAACAATTCGGTTGACTACGGCTCAAGCCTTACTGAAATTTCTTGATGCTCAGTATGTTGAATTGGACGGGGAAGA
ACACAAATTTGTCAAAGGGGTTTACGGTATATTTGGCCACGGTAACCTTCTTGGTATCGGTGAAGCTCTGGAAAATATGA
AGGATCTTTCTTTAACGTTCTACCAGGGACACAACGAACAGGGCATTGTTCATGCCGCTACCGCCTATGCAAAACAAAAA
AACCGCCTCGAAATATTCGCCTGTACCAGCTCCATTGGTCCTGGAGCACTTAATATGGTTGTCGGAGCTGCAACGGCCAC
CGTAAATAGGATTCCTGTTTTGTTGTTACCGGGAGATACTTTCGCTGACAGACAACCCGATCCCGTTCTTCAACAAATTG
AAATGCCTTACAATCCAACAATAACGTCTAATGATGCTTTTAAGGCTGTAAGCAAATACTGGGACAGGATAGAAAGGCCT
GAGCAGCTTATGTCCGCTGCTCTGAATGCAATGAGGGTTCTTACAGATCCAGCCGAAACAGGAGCGGTTACCCTCTGTCT
TCCACAGGACGTTCAGGGAGAAGCTTACGATTACCCGGTCGAATTTTTCGAAAAGAGGGTTCATCATATAGACCGCCGTC
CACCTTCTCTACCCGCTCTTGAGAGAGCTGTCGATTTGATCCTTCAAAAGAAGAAACCTTTGATCATAGCCGGTGGAGGT
GTGCATTATTCACTAGCAGTAGAAGAGCTTCGAGAATTTGCGGAAGCCTTCAACATTCCGGTGAGCGTAACGCAGGCCGG
TAAGAGCGTTATGTTGTGGGATCATCCTTTAAATATGGGAGGCGTCGGAACTACCGGTACCCTGGCTGCAAATATTCTTG
CAAAAGAAGCAGACCTGATTATAGCTATCGGTACGCGCCTCATGGATTTCCCAACTGCTTCCAAAACAGCTTTCCAGAAT
CCGGATGTCAAAATTTTGAGCATAAACGTAAATCCATTCGATGGTCTGAAAATGGATGCAGAGTATTTGAATGCTGATGC
AAAACTTGCCCTTAAAGAACTCAAAAGAATCCTTAAGGAGAAAAGCTATCGCTCCGGACATTCCGAAGAAAGGCTGAAAG
AACTCAAAGCTCAGTGGGATCGTGAGGTAGATAGACTTTATTCTTTGAGATCTGATAAAGGAATTCCTCAAACAGCCGCA
CTTGGTGTCATAAATGAATTCGCGACAGAAAATGACATCTTTGTTGCTGCCGCTGGCAGTCTACCGGGAGATCTCCACAG
ACTCTGGAGATGTAAGGGAATCAAGACGTATCACATGGAATACGGTTTTTCATGTATGGGTTACGAGGTTTCCGGTACCT
TCGGTGTTAAGATGGCTGAACCCGAAAAAGAGGCATTCGCTCTTCTGAGCGATGGAAGCTTCCTCATGCTCCATTCCGAA
CTCATCACCAGTCTTCAAGAAGGCAAAAAGATAAACGTGATAATTCTCGACAACGGAGGCTTCCAGAGTATAAACAGCTT
GCAAAAAGGACATGGAAGTAAAACAGGTTTTGGAAACGAATTACGTTATCGTTCTAAAACCACCGGAAAACTTGATGGAG
ACTATATGAAGATCGATTTCGCAGGTATAGCAAAAAGTCTTGGCGCAAAAGCCTACACGGCAAGAACTATTGAAGAACTG
AAAGACGCTCTCGAAAAAGCTAGAAAAGAAGACATAAGTGTTCTTATAGACGTTAAGGTACTTCCCGGCACGCAATCAGG
AGGTTATGAGTCCTGGTGGAGAGTTGGCGTTGCAGAGGTTTCAGAAAGTGATACCGTAAGGAAAGCCTTTGAAGAAAACC
AGAAATACATGAAGAAAGCCAGAAAATGGTAA

Upstream 100 bases:

>100_bases
TCTTGATGATAATCCTTACATAACACCGGTTTTCGAACCAGAACATACATGGGTCATGAACAAGAATGCGCCCATATGGA
CAAAGAGAGGTGAATGATCC

Downstream 100 bases:

>100_bases
TCGAGGGGGAGTTCTTGTGGTCGTAAGAGAGGATATAGGGTTAGGAATTGCTCCGATTGGATGGACCAACGACGACATGC
CAGAACTTGGTGGAGATATT

Product: thiamine pyrophosphate protein central region

Products: pyruvate; 2-aceto-2-hydroxy-butyrate; CO2 [C]

Alternate protein names: THcHDO hydrolase [H]

Number of amino acids: Translated: 623; Mature: 623

Protein sequence:

>623_residues
MKTIRLTTAQALLKFLDAQYVELDGEEHKFVKGVYGIFGHGNLLGIGEALENMKDLSLTFYQGHNEQGIVHAATAYAKQK
NRLEIFACTSSIGPGALNMVVGAATATVNRIPVLLLPGDTFADRQPDPVLQQIEMPYNPTITSNDAFKAVSKYWDRIERP
EQLMSAALNAMRVLTDPAETGAVTLCLPQDVQGEAYDYPVEFFEKRVHHIDRRPPSLPALERAVDLILQKKKPLIIAGGG
VHYSLAVEELREFAEAFNIPVSVTQAGKSVMLWDHPLNMGGVGTTGTLAANILAKEADLIIAIGTRLMDFPTASKTAFQN
PDVKILSINVNPFDGLKMDAEYLNADAKLALKELKRILKEKSYRSGHSEERLKELKAQWDREVDRLYSLRSDKGIPQTAA
LGVINEFATENDIFVAAAGSLPGDLHRLWRCKGIKTYHMEYGFSCMGYEVSGTFGVKMAEPEKEAFALLSDGSFLMLHSE
LITSLQEGKKINVIILDNGGFQSINSLQKGHGSKTGFGNELRYRSKTTGKLDGDYMKIDFAGIAKSLGAKAYTARTIEEL
KDALEKARKEDISVLIDVKVLPGTQSGGYESWWRVGVAEVSESDTVRKAFEENQKYMKKARKW

Sequences:

>Translated_623_residues
MKTIRLTTAQALLKFLDAQYVELDGEEHKFVKGVYGIFGHGNLLGIGEALENMKDLSLTFYQGHNEQGIVHAATAYAKQK
NRLEIFACTSSIGPGALNMVVGAATATVNRIPVLLLPGDTFADRQPDPVLQQIEMPYNPTITSNDAFKAVSKYWDRIERP
EQLMSAALNAMRVLTDPAETGAVTLCLPQDVQGEAYDYPVEFFEKRVHHIDRRPPSLPALERAVDLILQKKKPLIIAGGG
VHYSLAVEELREFAEAFNIPVSVTQAGKSVMLWDHPLNMGGVGTTGTLAANILAKEADLIIAIGTRLMDFPTASKTAFQN
PDVKILSINVNPFDGLKMDAEYLNADAKLALKELKRILKEKSYRSGHSEERLKELKAQWDREVDRLYSLRSDKGIPQTAA
LGVINEFATENDIFVAAAGSLPGDLHRLWRCKGIKTYHMEYGFSCMGYEVSGTFGVKMAEPEKEAFALLSDGSFLMLHSE
LITSLQEGKKINVIILDNGGFQSINSLQKGHGSKTGFGNELRYRSKTTGKLDGDYMKIDFAGIAKSLGAKAYTARTIEEL
KDALEKARKEDISVLIDVKVLPGTQSGGYESWWRVGVAEVSESDTVRKAFEENQKYMKKARKW
>Mature_623_residues
MKTIRLTTAQALLKFLDAQYVELDGEEHKFVKGVYGIFGHGNLLGIGEALENMKDLSLTFYQGHNEQGIVHAATAYAKQK
NRLEIFACTSSIGPGALNMVVGAATATVNRIPVLLLPGDTFADRQPDPVLQQIEMPYNPTITSNDAFKAVSKYWDRIERP
EQLMSAALNAMRVLTDPAETGAVTLCLPQDVQGEAYDYPVEFFEKRVHHIDRRPPSLPALERAVDLILQKKKPLIIAGGG
VHYSLAVEELREFAEAFNIPVSVTQAGKSVMLWDHPLNMGGVGTTGTLAANILAKEADLIIAIGTRLMDFPTASKTAFQN
PDVKILSINVNPFDGLKMDAEYLNADAKLALKELKRILKEKSYRSGHSEERLKELKAQWDREVDRLYSLRSDKGIPQTAA
LGVINEFATENDIFVAAAGSLPGDLHRLWRCKGIKTYHMEYGFSCMGYEVSGTFGVKMAEPEKEAFALLSDGSFLMLHSE
LITSLQEGKKINVIILDNGGFQSINSLQKGHGSKTGFGNELRYRSKTTGKLDGDYMKIDFAGIAKSLGAKAYTARTIEEL
KDALEKARKEDISVLIDVKVLPGTQSGGYESWWRVGVAEVSESDTVRKAFEENQKYMKKARKW

Specific function: Involved in the cleavage of the C1-C2 bond of 3D- (3,5/4)-trihydroxycyclohexane-1,2-dione (THcHDO) to yield 5-deoxy- glucuronate (5DG) [H]

COG id: COG3962

COG function: function code E; Acetolactate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=588, Percent_Identity=20.7482993197279, Blast_Score=72, Evalue=2e-12,
Organism=Escherichia coli, GI87081685, Length=574, Percent_Identity=26.4808362369338, Blast_Score=130, Evalue=2e-31,
Organism=Escherichia coli, GI1790104, Length=586, Percent_Identity=22.1843003412969, Blast_Score=117, Evalue=3e-27,
Organism=Escherichia coli, GI1787096, Length=503, Percent_Identity=25.2485089463221, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1786717, Length=512, Percent_Identity=23.2421875, Blast_Score=96, Evalue=6e-21,
Organism=Escherichia coli, GI1788716, Length=522, Percent_Identity=22.9885057471264, Blast_Score=80, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI17531299, Length=509, Percent_Identity=23.5756385068762, Blast_Score=87, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17531301, Length=509, Percent_Identity=23.5756385068762, Blast_Score=87, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6323755, Length=599, Percent_Identity=23.0383973288815, Blast_Score=109, Evalue=1e-24,
Organism=Drosophila melanogaster, GI19922626, Length=574, Percent_Identity=22.6480836236934, Blast_Score=91, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: 2.2.1.6 [C]

Molecular weight: Translated: 68892; Mature: 68892

Theoretical pI: Translated: 6.85; Mature: 6.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTIRLTTAQALLKFLDAQYVELDGEEHKFVKGVYGIFGHGNLLGIGEALENMKDLSLTF
CCEEEEHHHHHHHHHHCCCEEECCCCHHHHHHHHHHHCCCCCEEEHHHHHHHHHHCEEEE
YQGHNEQGIVHAATAYAKQKNRLEIFACTSSIGPGALNMVVGAATATVNRIPVLLLPGDT
EECCCCCCEEEHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHHHHHHCCCEEEEECCCC
FADRQPDPVLQQIEMPYNPTITSNDAFKAVSKYWDRIERPEQLMSAALNAMRVLTDPAET
CCCCCCCHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCC
GAVTLCLPQDVQGEAYDYPVEFFEKRVHHIDRRPPSLPALERAVDLILQKKKPLIIAGGG
CEEEEEECCCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCEEEECCC
VHYSLAVEELREFAEAFNIPVSVTQAGKSVMLWDHPLNMGGVGTTGTLAANILAKEADLI
EEEHHHHHHHHHHHHHHCCCEEEECCCCEEEEEECCCCCCCCCCHHHHHHHHHHCCCCEE
IAIGTRLMDFPTASKTAFQNPDVKILSINVNPFDGLKMDAEYLNADAKLALKELKRILKE
EEECCHHHCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCHHHHCCHHHHHHHHHHHHHHH
KSYRSGHSEERLKELKAQWDREVDRLYSLRSDKGIPQTAALGVINEFATENDIFVAAAGS
HHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCEEEEECCC
LPGDLHRLWRCKGIKTYHMEYGFSCMGYEVSGTFGVKMAEPEKEAFALLSDGSFLMLHSE
CCHHHHHHHHHCCCEEEEEECCCEEEEEEECCEEEEEECCCCHHHEEEECCCCEEEEHHH
LITSLQEGKKINVIILDNGGFQSINSLQKGHGSKTGFGNELRYRSKTTGKLDGDYMKIDF
HHHHHHCCCEEEEEEECCCCCHHHHHHHHCCCCCCCCCCCEEECCCCCCCCCCCEEEEEH
AGIAKSLGAKAYTARTIEELKDALEKARKEDISVLIDVKVLPGTQSGGYESWWRVGVAEV
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEECCCCCCCCHHHHHHHHHHC
SESDTVRKAFEENQKYMKKARKW
CCCHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKTIRLTTAQALLKFLDAQYVELDGEEHKFVKGVYGIFGHGNLLGIGEALENMKDLSLTF
CCEEEEHHHHHHHHHHCCCEEECCCCHHHHHHHHHHHCCCCCEEEHHHHHHHHHHCEEEE
YQGHNEQGIVHAATAYAKQKNRLEIFACTSSIGPGALNMVVGAATATVNRIPVLLLPGDT
EECCCCCCEEEHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHHHHHHCCCEEEEECCCC
FADRQPDPVLQQIEMPYNPTITSNDAFKAVSKYWDRIERPEQLMSAALNAMRVLTDPAET
CCCCCCCHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCC
GAVTLCLPQDVQGEAYDYPVEFFEKRVHHIDRRPPSLPALERAVDLILQKKKPLIIAGGG
CEEEEEECCCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCEEEECCC
VHYSLAVEELREFAEAFNIPVSVTQAGKSVMLWDHPLNMGGVGTTGTLAANILAKEADLI
EEEHHHHHHHHHHHHHHCCCEEEECCCCEEEEEECCCCCCCCCCHHHHHHHHHHCCCCEE
IAIGTRLMDFPTASKTAFQNPDVKILSINVNPFDGLKMDAEYLNADAKLALKELKRILKE
EEECCHHHCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCHHHHCCHHHHHHHHHHHHHHH
KSYRSGHSEERLKELKAQWDREVDRLYSLRSDKGIPQTAALGVINEFATENDIFVAAAGS
HHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCEEEEECCC
LPGDLHRLWRCKGIKTYHMEYGFSCMGYEVSGTFGVKMAEPEKEAFALLSDGSFLMLHSE
CCHHHHHHHHHCCCEEEEEECCCEEEEEEECCEEEEEECCCCHHHEEEECCCCEEEEHHH
LITSLQEGKKINVIILDNGGFQSINSLQKGHGSKTGFGNELRYRSKTTGKLDGDYMKIDF
HHHHHHCCCEEEEEEECCCCCHHHHHHHHCCCCCCCCCCCEEECCCCCCCCCCCEEEEEH
AGIAKSLGAKAYTARTIEELKDALEKARKEDISVLIDVKVLPGTQSGGYESWWRVGVAEV
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEECCCCCCCCHHHHHHHHHHC
SESDTVRKAFEENQKYMKKARKW
CCCHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD [C]

Metal ions: Mg2+ [C]

Kcat value (1/min): 4002 [C]

Specific activity: 53

Km value (mM): 7.6 {pyruvate}} 7 {pyruvate}} 2.9 {pyruvate}} 2.6 {pyruvate}} 1.3 {pyruvate}} 1 {pyruvate}} 5 {2-oxobutanoate}} 1 {2-oxobutanoate}} [C]

Substrates: acetolactate; CO2; pyruvate; 2-oxobutanoate [C]

Specific reaction: acetolactate + CO2 = 2 pyruvate pyruvate + 2-oxobutanoate = 2-aceto-2-hydroxy-butyrate + CO2 [C]

General reaction: C-C bond formation; decarboxylation [C]

Inhibitor: @OXOB02.txt*2-oxobutanoate!; chlorimuronethyl; chlorsulphuron; Ile; imazapyr; Leu; Val [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA