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The map label for this gene is rsuA [C]

Identifier: 239617259

GI number: 239617259

Start: 912330

End: 912947

Strand: Direct

Name: rsuA [C]

Synonym: Kole_0867

Alternate gene names: 239617259

Gene position: 912330-912947 (Clockwise)

Preceding gene: 239617258

Following gene: 239617260

Centisome position: 39.63

GC content: 43.37

Gene sequence:

>618_bases
GTGACGGAACCATGGTTTGAAATTGATCCCGTAAAAGATTCGATCACACTCGACGGGAAGAAAGTAAGGCCCAGAAAGCA
ACAGCGAGTGGTTTATGCTTTGAACAAGCCGCGAGGTTATCTCAGTAGTATGAGCGATGATAGAGGTCGGAAGACAATTG
CCGATCTTATAAAGGGGAAAATACCGGAAAAAGTCTTTCACGTTGGCAGGCTCGATATGGATACCGAAGGGTTGATAATC
CTCACCAATGACGGTGAGCTGGCCAGAAAACTCTCGCATCCTTCTTCTAAAATAAGGAAGACCTATGTTGCCAGGGTTAC
TGGAAAGCTGAGCCACCACGAAATAAAACGTTTGGAGAATGGCGTGGTTTTGAGAGACGGATTTAAAACTTCCGGGGCTT
CTATCAGGGTTATTCGGGCTTCACTAAGAGATACCCTAGTCGAAATAATCATTCATGAAGGGCACAAAAGAGAAATCAGG
GAAATGTTTAAAGTGTTCGACAAACACGTCCTTTACCTCAAAAGAACGGCTATTGGCAGCTTGAAGGTAGATATCGTTCC
AAACCCCGGAGACATAAAAAAACTAACTGGACAAGAAATACAAAAACTGCTAAAATAA

Upstream 100 bases:

>100_bases
TCTGAAACTTCAGAGGTTTCTTCAACTGAACAGCCACCTGTCGAGGCGGAAAGCGGGGGAAGCGATAAAGGGAAATAGGG
TTCTCATAAACGGTAAGCCC

Downstream 100 bases:

>100_bases
AGCGCAATACTAGTTAAAAGCTGGTGAAAAGATGCATAAAACCATCTCCTTTATTCCCACAAATTAATAACTTCTAGTAC
AGACCCTTAACTTTTATTCT

Product: pseudouridine synthase

Products: pseudouridine 5'-phosphate; H2O [C]

Alternate protein names: RNA pseudouridylate synthase; RNA-uridine isomerase [H]

Number of amino acids: Translated: 205; Mature: 204

Protein sequence:

>205_residues
MTEPWFEIDPVKDSITLDGKKVRPRKQQRVVYALNKPRGYLSSMSDDRGRKTIADLIKGKIPEKVFHVGRLDMDTEGLII
LTNDGELARKLSHPSSKIRKTYVARVTGKLSHHEIKRLENGVVLRDGFKTSGASIRVIRASLRDTLVEIIIHEGHKREIR
EMFKVFDKHVLYLKRTAIGSLKVDIVPNPGDIKKLTGQEIQKLLK

Sequences:

>Translated_205_residues
MTEPWFEIDPVKDSITLDGKKVRPRKQQRVVYALNKPRGYLSSMSDDRGRKTIADLIKGKIPEKVFHVGRLDMDTEGLII
LTNDGELARKLSHPSSKIRKTYVARVTGKLSHHEIKRLENGVVLRDGFKTSGASIRVIRASLRDTLVEIIIHEGHKREIR
EMFKVFDKHVLYLKRTAIGSLKVDIVPNPGDIKKLTGQEIQKLLK
>Mature_204_residues
TEPWFEIDPVKDSITLDGKKVRPRKQQRVVYALNKPRGYLSSMSDDRGRKTIADLIKGKIPEKVFHVGRLDMDTEGLIIL
TNDGELARKLSHPSSKIRKTYVARVTGKLSHHEIKRLENGVVLRDGFKTSGASIRVIRASLRDTLVEIIIHEGHKREIRE
MFKVFDKHVLYLKRTAIGSLKVDIVPNPGDIKKLTGQEIQKLLK

Specific function: Responsible For Synthesis Of Pseudouridine From Uracil-516 In 16s Ribosomal RNA. [C]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788510, Length=200, Percent_Identity=29.5, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI87081838, Length=189, Percent_Identity=31.2169312169312, Blast_Score=91, Evalue=6e-20,
Organism=Escherichia coli, GI1787524, Length=154, Percent_Identity=33.7662337662338, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1790453, Length=194, Percent_Identity=30.9278350515464, Blast_Score=75, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: 4.2.1.70 [C]

Molecular weight: Translated: 23382; Mature: 23251

Theoretical pI: Translated: 10.76; Mature: 10.76

Prosite motif: PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEPWFEIDPVKDSITLDGKKVRPRKQQRVVYALNKPRGYLSSMSDDRGRKTIADLIKGK
CCCCCCEECCCCCCEEECCCCCCCCCCCEEEEEECCCCHHHHCCCCCCHHHHHHHHHHCC
IPEKVFHVGRLDMDTEGLIILTNDGELARKLSHPSSKIRKTYVARVTGKLSHHEIKRLEN
CCHHHHHHCCCCCCCCCEEEEECCHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHHC
GVVLRDGFKTSGASIRVIRASLRDTLVEIIIHEGHKREIREMFKVFDKHVLYLKRTAIGS
CEEEECCCCCCCCEEEEHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCC
LKVDIVPNPGDIKKLTGQEIQKLLK
EEEEEECCCCCHHHCCHHHHHHHHC
>Mature Secondary Structure 
TEPWFEIDPVKDSITLDGKKVRPRKQQRVVYALNKPRGYLSSMSDDRGRKTIADLIKGK
CCCCCEECCCCCCEEECCCCCCCCCCCEEEEEECCCCHHHHCCCCCCHHHHHHHHHHCC
IPEKVFHVGRLDMDTEGLIILTNDGELARKLSHPSSKIRKTYVARVTGKLSHHEIKRLEN
CCHHHHHHCCCCCCCCCEEEEECCHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHHC
GVVLRDGFKTSGASIRVIRASLRDTLVEIIIHEGHKREIREMFKVFDKHVLYLKRTAIGS
CEEEECCCCCCCCEEEEHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCC
LKVDIVPNPGDIKKLTGQEIQKLLK
EEEEEECCCCCHHHCCHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose phosphate [C]

Specific reaction: uracil + D-ribose phosphate = pseudouridine 5'-phosphate + H2O [C]

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9537320 [H]