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The map label for this gene is hemK [C]

Identifier: 239617253

GI number: 239617253

Start: 907684

End: 908532

Strand: Direct

Name: hemK [C]

Synonym: Kole_0861

Alternate gene names: 239617253

Gene position: 907684-908532 (Clockwise)

Preceding gene: 239617252

Following gene: 239617256

Centisome position: 39.43

GC content: 41.46

Gene sequence:

>849_bases
ATGAAACTCAAAGAGTTGTTTGAAGGAATCACGTTCGAACTCAAAGAAGCGGGGATTGAAAATCCTCGCTTCGTTACTTT
TACTTTACTTGAAAGTATTGCAATGATAGAAAAACATAAAGTGTTTGTTGATACTGCCACAGAAATCGATACAAAAACCT
GTAAGCTTCTATTCGACGCGGTGAAAAGGCTAAAGGCTGGTGAACCGCTGGATTATATCATTGGCTGGAAGTATTTTCTT
GGAGCCAAGCTTAACCTTGATTCAAGGGTTCTAATCCCAAGGCCGGAAACAGAGGAACTCGTGGAGATGATCATAAACGA
GCATAAAGGTAAGAACGTAAAAGCCTTCGCAGACGTAGGCACCGGTAGCGGTGCCATAGCAATCGCTCTAGCAAAACACT
TCCCAGCATCAAAGATCTACGCCACCGACATCTCCAAACCAGCTTTAGAACTTGCATTCGAAAACGCGAAAATCAATGGA
GTAGAAGGAAGAATAGCCTTCCTTCACGGGAAAAATCTTAACCCCCTTGAAGCTTATATGGATGAAATAGAAATTATAGT
TTCAAATCCACCGTATGTGAAAACCACGGTCCTTGAATCACTCGATAAAAGGGTCAAAGACTATGAGCCTATAATCGCTT
TAGATGGCGGTGAAGATGGAATGAATTTTTTCAGGGAGTTTATAAAAGTTCTCCCAGAGGGAAAATTCGTGTATCTTGAA
ATTGCCACTTACTCCCGGAATCCGTTGAAAGACTTTCTTAAGAAATACCGGAAACGTTACACAGTCAAATTCAGAAGAGA
TCTCAGCGGTAAAATCCGGTTCGCTATACTTCGCCCAGAAGGGAAATAA

Upstream 100 bases:

>100_bases
TAGAAGGTGTCAACAAAGTTGATGTTGAACTGACTTTCGATCCACCATGGACACCCGAAAAAGCTTCTGACGAAGTAAAA
AAAATCTTTGGAATATAAGA

Downstream 100 bases:

>100_bases
TCCTTTCTGAAAACTCTGCTAAATCGCACTCGTACTTCTTGTCTGCTATATAATCAAGCCCTGTTTCACCCTGATTGATT
ATGAGCAATTTTGCTCCCTT

Product: modification methylase, HemK family

Products: NA

Alternate protein names: M.Ssp6803HemKP [H]

Number of amino acids: Translated: 282; Mature: 282

Protein sequence:

>282_residues
MKLKELFEGITFELKEAGIENPRFVTFTLLESIAMIEKHKVFVDTATEIDTKTCKLLFDAVKRLKAGEPLDYIIGWKYFL
GAKLNLDSRVLIPRPETEELVEMIINEHKGKNVKAFADVGTGSGAIAIALAKHFPASKIYATDISKPALELAFENAKING
VEGRIAFLHGKNLNPLEAYMDEIEIIVSNPPYVKTTVLESLDKRVKDYEPIIALDGGEDGMNFFREFIKVLPEGKFVYLE
IATYSRNPLKDFLKKYRKRYTVKFRRDLSGKIRFAILRPEGK

Sequences:

>Translated_282_residues
MKLKELFEGITFELKEAGIENPRFVTFTLLESIAMIEKHKVFVDTATEIDTKTCKLLFDAVKRLKAGEPLDYIIGWKYFL
GAKLNLDSRVLIPRPETEELVEMIINEHKGKNVKAFADVGTGSGAIAIALAKHFPASKIYATDISKPALELAFENAKING
VEGRIAFLHGKNLNPLEAYMDEIEIIVSNPPYVKTTVLESLDKRVKDYEPIIALDGGEDGMNFFREFIKVLPEGKFVYLE
IATYSRNPLKDFLKKYRKRYTVKFRRDLSGKIRFAILRPEGK
>Mature_282_residues
MKLKELFEGITFELKEAGIENPRFVTFTLLESIAMIEKHKVFVDTATEIDTKTCKLLFDAVKRLKAGEPLDYIIGWKYFL
GAKLNLDSRVLIPRPETEELVEMIINEHKGKNVKAFADVGTGSGAIAIALAKHFPASKIYATDISKPALELAFENAKING
VEGRIAFLHGKNLNPLEAYMDEIEIIVSNPPYVKTTVLESLDKRVKDYEPIIALDGGEDGMNFFREFIKVLPEGKFVYLE
IATYSRNPLKDFLKKYRKRYTVKFRRDLSGKIRFAILRPEGK

Specific function: Probable protein methyltransferase. May methylate a Gln residue in target proteins [H]

COG id: COG2890

COG function: function code J; Methylase of polypeptide chain release factors

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hemK family [H]

Homologues:

Organism=Homo sapiens, GI7705409, Length=230, Percent_Identity=32.1739130434783, Blast_Score=100, Evalue=2e-21,
Organism=Escherichia coli, GI1787463, Length=227, Percent_Identity=30.8370044052863, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI87082085, Length=184, Percent_Identity=28.804347826087, Blast_Score=81, Evalue=8e-17,
Organism=Drosophila melanogaster, GI24582226, Length=221, Percent_Identity=32.579185520362, Blast_Score=103, Evalue=1e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR004556
- InterPro:   IPR019874
- InterPro:   IPR007848 [H]

Pfam domain/function: PF05175 MTS [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 32055; Mature: 32055

Theoretical pI: Translated: 9.30; Mature: 9.30

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLKELFEGITFELKEAGIENPRFVTFTLLESIAMIEKHKVFVDTATEIDTKTCKLLFDA
CCHHHHHCCCCCHHHHCCCCCCCEEHHHHHHHHHHHHHCEEEEECCCCCCHHHHHHHHHH
VKRLKAGEPLDYIIGWKYFLGAKLNLDSRVLIPRPETEELVEMIINEHKGKNVKAFADVG
HHHHHCCCCHHHHCCHHHHHCCEECCCCEEEECCCCHHHHHHHHHHHCCCCCEEEEEECC
TGSGAIAIALAKHFPASKIYATDISKPALELAFENAKINGVEGRIAFLHGKNLNPLEAYM
CCCCEEEEEEECCCCCCCEEECCCCCHHHHHEECCCEEECCCCEEEEEECCCCCHHHHHH
DEIEIIVSNPPYVKTTVLESLDKRVKDYEPIIALDGGEDGMNFFREFIKVLPEGKFVYLE
HHEEEEECCCCCHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHCCCCCEEEEE
IATYSRNPLKDFLKKYRKRYTVKFRRDLSGKIRFAILRPEGK
EEECCCCHHHHHHHHHHHHEEEEEECCCCCEEEEEEECCCCC
>Mature Secondary Structure
MKLKELFEGITFELKEAGIENPRFVTFTLLESIAMIEKHKVFVDTATEIDTKTCKLLFDA
CCHHHHHCCCCCHHHHCCCCCCCEEHHHHHHHHHHHHHCEEEEECCCCCCHHHHHHHHHH
VKRLKAGEPLDYIIGWKYFLGAKLNLDSRVLIPRPETEELVEMIINEHKGKNVKAFADVG
HHHHHCCCCHHHHCCHHHHHCCEECCCCEEEECCCCHHHHHHHHHHHCCCCCEEEEEECC
TGSGAIAIALAKHFPASKIYATDISKPALELAFENAKINGVEGRIAFLHGKNLNPLEAYM
CCCCEEEEEEECCCCCCCEEECCCCCHHHHHEECCCEEECCCCEEEEEECCCCCHHHHHH
DEIEIIVSNPPYVKTTVLESLDKRVKDYEPIIALDGGEDGMNFFREFIKVLPEGKFVYLE
HHEEEEECCCCCHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHCCCCCEEEEE
IATYSRNPLKDFLKKYRKRYTVKFRRDLSGKIRFAILRPEGK
EEECCCCHHHHHHHHHHHHEEEEEECCCCCEEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]