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The map label for this gene is yfiU [H]

Identifier: 239616539

GI number: 239616539

Start: 145355

End: 146737

Strand: Reverse

Name: yfiU [H]

Synonym: Kole_0131

Alternate gene names: 239616539

Gene position: 146737-145355 (Counterclockwise)

Preceding gene: 239616540

Following gene: 239616538

Centisome position: 6.37

GC content: 40.42

Gene sequence:

>1383_bases
ATGTCCATGTTCACGATACTTTTTGTAAACTTCATATCAGCGGTTGGCATAACGATTGTGGCACCTATTCTTGGAATAGC
AGGACAGATATTTGAGGTCGATCAGGGCTACGCTATGTGGTTGATGACGGGTTTTATGATGGCGTACGCCTCTTTCATGC
CAATGATCGGAAGAATGAGTGATAATTTTGGGAGAAAAAAGGGTTTCATAGCTTCGGCATCGATTTTTTCTTTTGGTCTT
CTCCTGAGCTTCTTATCTAATGACTTCAATATCGTTATTCTTGGGAGGATGATCCAGGGTTTCGGTGCCGGTGGGATTCT
GCCGGTGGCGAATGCCATGGCTGTTGAGCTTTACCAGGATTCTAAAGGTAAAGCCCTCGCGCTCGTTAATGCTACTTACG
GGCTTGGTGTTGTTGTCGGAGTAAACCTCGGAGGTATTATCTATGATCATCTTGGCTGGAAGTGGATGTTCATAGTTCCT
TTCATCCTGACGCTGATCGTTATTCCGATAGCTGTTTCTTTCTTGAAGGAGACATTAAAGTTTAAAAAGCATGAGGCTAT
AGATTACATTGGAAGTATTTTATTTGCGGTTGCTATAATTTCATTTATGTTTATGATGAAATACCTTGCGGTGGCTCCCT
TCTTTTCACCTGAGGTTTTAATTCCGATGATTGGCATGGTTATATCTTTTATTCTCTTTGTTATCAGGGAATTAAAGGTG
GAGAATCCGGTCATAGATATAAAGGATTTTAAGAATCCATCGTTCTTGATTTACAGCCTGATTGCTTTCTTCTTTGGTTT
TTCTATGTTCCTTTTTGTTACTTTCCTGCCTTCGTTTACCCAGACATTGCTTGGATACGGGGTCTCAGAATCTGTTTACG
CTATTGATCCCTTTGCGATAGCGATGATTTTCTTCGTCATGCTCGGAGGGATCTTAATAAAAAGATTTGGTGCAAGGAAA
TCAATGGTTATAGGGGCGCTTCTTTTTTCGATCTTCTCCTATCTTTTCGTAAAAATGGTAGATGATGGGCCTTCATTCTA
CATCTACTCTATACTCCTCGCATCGGGACTCGGAATTTCAATGACGCCGATGAATTACGTGATCATGGAAGAAGGTGGAA
AAGAAAAACAGGGTGTCTCTGCGGGAATAGCAAGTGTTATGAGGAGCCTTGGAGGAATCGTTGGACCTACAATAGCCGGG
ATAATAATAGCAAATGTCGATTTTAGCTCTATCTTTGTTATGGACAATCTTATAGAGGCTTATATGAAAATCTTCAGCAT
ATCGTTTTATTCAACGCTGATACAGGTGGCCTTGAGTATTTTGGGATTGTTTGCGTTTAGAAAAATATTGATAAAAGGAG
GTATCCGCAATGAGAAAATTTAA

Upstream 100 bases:

>100_bases
ATTACATATCTGCATTTTTTTCAATCGCCACAATTGACATGTAATATATAAAGGTGTATCATAATACCGACTGGTCGGTA
TTATGAAGGAGGTTGAAAGA

Downstream 100 bases:

>100_bases
GATGGTCACGGGGCTTTTTTTGGTAATGGTGATAGTGGCGAGTGCCGTGCTTTCGGTTTCACTCAACGATATATTGGTAA
CATCAGAAAATATGAAGACA

Product: major facilitator superfamily MFS_1

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 460; Mature: 459

Protein sequence:

>460_residues
MSMFTILFVNFISAVGITIVAPILGIAGQIFEVDQGYAMWLMTGFMMAYASFMPMIGRMSDNFGRKKGFIASASIFSFGL
LLSFLSNDFNIVILGRMIQGFGAGGILPVANAMAVELYQDSKGKALALVNATYGLGVVVGVNLGGIIYDHLGWKWMFIVP
FILTLIVIPIAVSFLKETLKFKKHEAIDYIGSILFAVAIISFMFMMKYLAVAPFFSPEVLIPMIGMVISFILFVIRELKV
ENPVIDIKDFKNPSFLIYSLIAFFFGFSMFLFVTFLPSFTQTLLGYGVSESVYAIDPFAIAMIFFVMLGGILIKRFGARK
SMVIGALLFSIFSYLFVKMVDDGPSFYIYSILLASGLGISMTPMNYVIMEEGGKEKQGVSAGIASVMRSLGGIVGPTIAG
IIIANVDFSSIFVMDNLIEAYMKIFSISFYSTLIQVALSILGLFAFRKILIKGGIRNEKI

Sequences:

>Translated_460_residues
MSMFTILFVNFISAVGITIVAPILGIAGQIFEVDQGYAMWLMTGFMMAYASFMPMIGRMSDNFGRKKGFIASASIFSFGL
LLSFLSNDFNIVILGRMIQGFGAGGILPVANAMAVELYQDSKGKALALVNATYGLGVVVGVNLGGIIYDHLGWKWMFIVP
FILTLIVIPIAVSFLKETLKFKKHEAIDYIGSILFAVAIISFMFMMKYLAVAPFFSPEVLIPMIGMVISFILFVIRELKV
ENPVIDIKDFKNPSFLIYSLIAFFFGFSMFLFVTFLPSFTQTLLGYGVSESVYAIDPFAIAMIFFVMLGGILIKRFGARK
SMVIGALLFSIFSYLFVKMVDDGPSFYIYSILLASGLGISMTPMNYVIMEEGGKEKQGVSAGIASVMRSLGGIVGPTIAG
IIIANVDFSSIFVMDNLIEAYMKIFSISFYSTLIQVALSILGLFAFRKILIKGGIRNEKI
>Mature_459_residues
SMFTILFVNFISAVGITIVAPILGIAGQIFEVDQGYAMWLMTGFMMAYASFMPMIGRMSDNFGRKKGFIASASIFSFGLL
LSFLSNDFNIVILGRMIQGFGAGGILPVANAMAVELYQDSKGKALALVNATYGLGVVVGVNLGGIIYDHLGWKWMFIVPF
ILTLIVIPIAVSFLKETLKFKKHEAIDYIGSILFAVAIISFMFMMKYLAVAPFFSPEVLIPMIGMVISFILFVIRELKVE
NPVIDIKDFKNPSFLIYSLIAFFFGFSMFLFVTFLPSFTQTLLGYGVSESVYAIDPFAIAMIFFVMLGGILIKRFGARKS
MVIGALLFSIFSYLFVKMVDDGPSFYIYSILLASGLGISMTPMNYVIMEEGGKEKQGVSAGIASVMRSLGGIVGPTIAGI
IIANVDFSSIFVMDNLIEAYMKIFSISFYSTLIQVALSILGLFAFRKILIKGGIRNEKI

Specific function: Translocase That Confers Resistance To Substances Of High Hydrophobicity. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. TCR/tet family [H]

Homologues:

Organism=Escherichia coli, GI1789042, Length=391, Percent_Identity=24.5524296675192, Blast_Score=105, Evalue=6e-24,
Organism=Escherichia coli, GI1788710, Length=326, Percent_Identity=27.3006134969325, Blast_Score=96, Evalue=4e-21,
Organism=Escherichia coli, GI87081983, Length=383, Percent_Identity=21.9321148825065, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI1790195, Length=295, Percent_Identity=26.4406779661017, Blast_Score=79, Evalue=5e-16,
Organism=Saccharomyces cerevisiae, GI6323735, Length=259, Percent_Identity=25.8687258687259, Blast_Score=87, Evalue=6e-18,
Organism=Saccharomyces cerevisiae, GI6319770, Length=384, Percent_Identity=23.1770833333333, Blast_Score=79, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6325455, Length=127, Percent_Identity=32.2834645669291, Blast_Score=74, Evalue=7e-14,
Organism=Saccharomyces cerevisiae, GI6321663, Length=121, Percent_Identity=31.404958677686, Blast_Score=64, Evalue=5e-11,
Organism=Saccharomyces cerevisiae, GI6324954, Length=409, Percent_Identity=22.4938875305623, Blast_Score=63, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR001411 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 50548; Mature: 50416

Theoretical pI: Translated: 9.48; Mature: 9.48

Prosite motif: PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
6.1 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
5.9 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSMFTILFVNFISAVGITIVAPILGIAGQIFEVDQGYAMWLMTGFMMAYASFMPMIGRMS
CCHHHHHHHHHHHHHHHHHHHHHHHHCCHHEECCCCHHHHHHHHHHHHHHHHHHHHHHHH
DNFGRKKGFIASASIFSFGLLLSFLSNDFNIVILGRMIQGFGAGGILPVANAMAVELYQD
HCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEHHHHCCCCCCCHHHHHHHHHHHEEC
SKGKALALVNATYGLGVVVGVNLGGIIYDHLGWKWMFIVPFILTLIVIPIAVSFLKETLK
CCCCEEEEEECCCCCCEEEECCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
FKKHEAIDYIGSILFAVAIISFMFMMKYLAVAPFFSPEVLIPMIGMVISFILFVIRELKV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
ENPVIDIKDFKNPSFLIYSLIAFFFGFSMFLFVTFLPSFTQTLLGYGVSESVYAIDPFAI
CCCEEEHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECHHHH
AMIFFVMLGGILIKRFGARKSMVIGALLFSIFSYLFVKMVDDGPSFYIYSILLASGLGIS
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCE
MTPMNYVIMEEGGKEKQGVSAGIASVMRSLGGIVGPTIAGIIIANVDFSSIFVMDNLIEA
ECCCEEEEEECCCCCCCHHHHHHHHHHHHHCCHHCHHHHHHHEECCCHHHHHHHHHHHHH
YMKIFSISFYSTLIQVALSILGLFAFRKILIKGGIRNEKI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
SMFTILFVNFISAVGITIVAPILGIAGQIFEVDQGYAMWLMTGFMMAYASFMPMIGRMS
CHHHHHHHHHHHHHHHHHHHHHHHHCCHHEECCCCHHHHHHHHHHHHHHHHHHHHHHHH
DNFGRKKGFIASASIFSFGLLLSFLSNDFNIVILGRMIQGFGAGGILPVANAMAVELYQD
HCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEHHHHCCCCCCCHHHHHHHHHHHEEC
SKGKALALVNATYGLGVVVGVNLGGIIYDHLGWKWMFIVPFILTLIVIPIAVSFLKETLK
CCCCEEEEEECCCCCCEEEECCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
FKKHEAIDYIGSILFAVAIISFMFMMKYLAVAPFFSPEVLIPMIGMVISFILFVIRELKV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
ENPVIDIKDFKNPSFLIYSLIAFFFGFSMFLFVTFLPSFTQTLLGYGVSESVYAIDPFAI
CCCEEEHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECHHHH
AMIFFVMLGGILIKRFGARKSMVIGALLFSIFSYLFVKMVDDGPSFYIYSILLASGLGIS
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCE
MTPMNYVIMEEGGKEKQGVSAGIASVMRSLGGIVGPTIAGIIIANVDFSSIFVMDNLIEA
ECCCEEEEEECCCCCCCHHHHHHHHHHHHHCCHHCHHHHHHHEECCCHHHHHHHHHHHHH
YMKIFSISFYSTLIQVALSILGLFAFRKILIKGGIRNEKI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8946165; 9384377 [H]