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The map label for this gene is speE

Identifier: 238925687

GI number: 238925687

Start: 3166260

End: 3167117

Strand: Reverse

Name: speE

Synonym: EUBREC_3344

Alternate gene names: 238925687

Gene position: 3167117-3166260 (Counterclockwise)

Preceding gene: 238925688

Following gene: 238925686

Centisome position: 91.81

GC content: 43.12

Gene sequence:

>858_bases
ATGCAAATGTGGTTTTCACAATATCATACTGTCAATGTCAAGCTGGATGTAAGGATACAGGAGCAGCTTTTTTCAGGGCA
GAGTGATTATCAGAAAATAGATGTGTTTAAATCATATGAGTTTGGCAAAATGGTCGCGCTGGATGGCGAGATTGTTTTTT
CGGACACAGATGAGTTTATATATGATGAGATGGTCACACATGTGCCGATGGCGGTCCACCCGAACGCAAAAAGGATACTT
ATAATAGGTGGCGGCGACGGCGGCGTGGCAAAGGAGCTCATCAAATATCCGTCTGTCGAGCATATTGATGTGGTTGAGAC
AGACAAGATGTTTGTCGATGTATGCAGGCGGTTTTTCCCCGAGGTAGCATGTGGACTTGAGGATGAAAGAATTTCCATGT
TTTATGATGATGGTCTGCGATTCTTACGCAGAAAGCATGATGAATATGATCTGATAATCAACGATTCCACAGACCCGCTC
GGACATACGGAGGGACTTTTCACAAAAGAGTTTTATGGAAGCTGTTATAAGGCACTAAAGGATGATGGAATCATGGTTTA
CCAGCACGGCAGTCCTTTTTATGATGAGGATGAGCTTGAGTGCCGGAAAATGCACAGAAAGGTGTATAAATCATTTCCTA
TAAGCAGGGTATATCAGGCACATATTCCAACCTGTCCGTCAGGCTACTGGCTGTTTGGGTTTGCATCGAAGAAATATCAT
CCGCTTTATGATCTGGATGCAAAAAGATGGGATGAACTGGGGCTTAAAACCTGGTATTATACAACACATTTACACAGGGG
AGCCTTCATGCTTCCGAAATATGTGGAGGATTTACTCGAGGAAGAGGAAACAAAGTAA

Upstream 100 bases:

>100_bases
CATTGTCTACGCAAAGGAAAAGGGCTGTTCAATGCAGGGAACAGAGGATCCTGCGGTAGAATATCTTAAGGTATTGGAGC
AGTAAACAGGAGGAAAAGCT

Downstream 100 bases:

>100_bases
AACAAATTAGAAAAACTATATCATAACCAAAGCGAATCAGGAGGGACAAAAATGAGCAGATTACTTATTATCGGATGTGG
TGGAGTTGCGCAGGTGGCAA

Product: spermidine synthase

Products: NA

Alternate protein names: Putrescine aminopropyltransferase; PAPT; SPDSY

Number of amino acids: Translated: 285; Mature: 285

Protein sequence:

>285_residues
MQMWFSQYHTVNVKLDVRIQEQLFSGQSDYQKIDVFKSYEFGKMVALDGEIVFSDTDEFIYDEMVTHVPMAVHPNAKRIL
IIGGGDGGVAKELIKYPSVEHIDVVETDKMFVDVCRRFFPEVACGLEDERISMFYDDGLRFLRRKHDEYDLIINDSTDPL
GHTEGLFTKEFYGSCYKALKDDGIMVYQHGSPFYDEDELECRKMHRKVYKSFPISRVYQAHIPTCPSGYWLFGFASKKYH
PLYDLDAKRWDELGLKTWYYTTHLHRGAFMLPKYVEDLLEEEETK

Sequences:

>Translated_285_residues
MQMWFSQYHTVNVKLDVRIQEQLFSGQSDYQKIDVFKSYEFGKMVALDGEIVFSDTDEFIYDEMVTHVPMAVHPNAKRIL
IIGGGDGGVAKELIKYPSVEHIDVVETDKMFVDVCRRFFPEVACGLEDERISMFYDDGLRFLRRKHDEYDLIINDSTDPL
GHTEGLFTKEFYGSCYKALKDDGIMVYQHGSPFYDEDELECRKMHRKVYKSFPISRVYQAHIPTCPSGYWLFGFASKKYH
PLYDLDAKRWDELGLKTWYYTTHLHRGAFMLPKYVEDLLEEEETK
>Mature_285_residues
MQMWFSQYHTVNVKLDVRIQEQLFSGQSDYQKIDVFKSYEFGKMVALDGEIVFSDTDEFIYDEMVTHVPMAVHPNAKRIL
IIGGGDGGVAKELIKYPSVEHIDVVETDKMFVDVCRRFFPEVACGLEDERISMFYDDGLRFLRRKHDEYDLIINDSTDPL
GHTEGLFTKEFYGSCYKALKDDGIMVYQHGSPFYDEDELECRKMHRKVYKSFPISRVYQAHIPTCPSGYWLFGFASKKYH
PLYDLDAKRWDELGLKTWYYTTHLHRGAFMLPKYVEDLLEEEETK

Specific function: Catalyzes the production of spermidine from putrescine and decarboxylated S-adenosylmethionine (dcSAM), which serves as an aminopropyl donor

COG id: COG0421

COG function: function code E; Spermidine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the spermidine/spermine synthase family

Homologues:

Organism=Homo sapiens, GI63253298, Length=285, Percent_Identity=34.0350877192982, Blast_Score=186, Evalue=2e-47,
Organism=Escherichia coli, GI1786312, Length=266, Percent_Identity=37.9699248120301, Blast_Score=191, Evalue=5e-50,
Organism=Caenorhabditis elegans, GI25154106, Length=270, Percent_Identity=36.2962962962963, Blast_Score=167, Evalue=5e-42,
Organism=Caenorhabditis elegans, GI212645616, Length=226, Percent_Identity=37.6106194690265, Blast_Score=150, Evalue=6e-37,
Organism=Saccharomyces cerevisiae, GI6323175, Length=269, Percent_Identity=33.0855018587361, Blast_Score=157, Evalue=2e-39,
Organism=Saccharomyces cerevisiae, GI6325326, Length=278, Percent_Identity=31.294964028777, Blast_Score=155, Evalue=5e-39,
Organism=Drosophila melanogaster, GI24645443, Length=279, Percent_Identity=32.6164874551971, Blast_Score=174, Evalue=5e-44,
Organism=Drosophila melanogaster, GI21355941, Length=216, Percent_Identity=34.2592592592593, Blast_Score=145, Evalue=4e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SPEE_EUBR3 (C4ZDU4)

Other databases:

- EMBL:   CP001107
- RefSeq:   YP_002939204.1
- ProteinModelPortal:   C4ZDU4
- GeneID:   7964561
- GenomeReviews:   CP001107_GR
- KEGG:   ere:EUBREC_3344
- ProtClustDB:   PRK00811
- HAMAP:   MF_00198
- InterPro:   IPR001045
- PANTHER:   PTHR11558
- TIGRFAMs:   TIGR00417

Pfam domain/function: PF01564 Spermine_synth

EC number: =2.5.1.16

Molecular weight: Translated: 33502; Mature: 33502

Theoretical pI: Translated: 5.12; Mature: 5.12

Prosite motif: PS01330 SPERMIDINE_SYNTHASE_1; PS51006 SPERMIDINE_SYNTHASE_2

Important sites: BINDING 31-31 BINDING 86-86 BINDING 106-106 BINDING 155-155 BINDING 158-158

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQMWFSQYHTVNVKLDVRIQEQLFSGQSDYQKIDVFKSYEFGKMVALDGEIVFSDTDEFI
CCCCCCCEEEEEEEEEEEEHHHHHCCCCCHHHHHHHHCCCCCEEEEECCEEEEECCHHHH
YDEMVTHVPMAVHPNAKRILIIGGGDGGVAKELIKYPSVEHIDVVETDKMFVDVCRRFFP
HHHHHHHCCEEECCCCCEEEEEECCCCHHHHHHHHCCCCCCEEEECHHHHHHHHHHHHHH
EVACGLEDERISMFYDDGLRFLRRKHDEYDLIINDSTDPLGHTEGLFTKEFYGSCYKALK
HHHCCCCCCCEEEEHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHC
DDGIMVYQHGSPFYDEDELECRKMHRKVYKSFPISRVYQAHIPTCPSGYWLFGFASKKYH
CCCEEEEECCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCCCEEEEECCCCCC
PLYDLDAKRWDELGLKTWYYTTHLHRGAFMLPKYVEDLLEEEETK
CCCCCCHHHHHHCCCEEEEEEEECCCCCCHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MQMWFSQYHTVNVKLDVRIQEQLFSGQSDYQKIDVFKSYEFGKMVALDGEIVFSDTDEFI
CCCCCCCEEEEEEEEEEEEHHHHHCCCCCHHHHHHHHCCCCCEEEEECCEEEEECCHHHH
YDEMVTHVPMAVHPNAKRILIIGGGDGGVAKELIKYPSVEHIDVVETDKMFVDVCRRFFP
HHHHHHHCCEEECCCCCEEEEEECCCCHHHHHHHHCCCCCCEEEECHHHHHHHHHHHHHH
EVACGLEDERISMFYDDGLRFLRRKHDEYDLIINDSTDPLGHTEGLFTKEFYGSCYKALK
HHHCCCCCCCEEEEHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHC
DDGIMVYQHGSPFYDEDELECRKMHRKVYKSFPISRVYQAHIPTCPSGYWLFGFASKKYH
CCCEEEEECCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCCCEEEEECCCCCC
PLYDLDAKRWDELGLKTWYYTTHLHRGAFMLPKYVEDLLEEEETK
CCCCCCHHHHHHCCCEEEEEEEECCCCCCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA