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The map label for this gene is glpK

Identifier: 238924938

GI number: 238924938

Start: 2461399

End: 2462895

Strand: Reverse

Name: glpK

Synonym: EUBREC_2589

Alternate gene names: 238924938

Gene position: 2462895-2461399 (Counterclockwise)

Preceding gene: 238924939

Following gene: 238924937

Centisome position: 71.39

GC content: 49.5

Gene sequence:

>1497_bases
ATGGCAAAGTATATTATGGCATTAGATGCGGGCACGACCAGCAACAGATGTATACTTTTCAATGAGAAGGGCGAGATGTG
CAGCGTGGCACAGAAGGAATTTACGCAGTTTTTCCCGAAGCCAGGCTGGGTGGAGCACGATGCAGAGGAAATCTGGGCCA
CACAGCTTGAGGTGGCAAAGGAGGCAATGGCAAATATACAGGCTACGGCAGCCGACATATGTGCGATAGGCATTACTAAC
CAGAGAGAGACCACGATTGTGTGGGACAAAAATACGGGAGAGCCGGTTTATCACGCAATAGTATGGCAGTGCAGGCGCAC
GGCAGAGTATGCGGATTCACTGAAGGAAAAGGGGCTTACCGAGACCTTCAGGAAAAAGACAGGTCTTGTGATAGATGCTT
ATTTTTCGGCAACTAAGCTCAAGTGGCTCCTTGACAATGTGCCGGGAGCAAGGGAGAGAGCCGAAAGAGGCGAGCTTTTG
TTTGGAACAGTCGAGACATGGCTTATATGGAAGCTCACTCAGGGGCAGGTGCATGTTACGGATTATTCCAATGCATCAAG
AACCATGATGTTTAATATCAATACCTTAAAGTGGGATGATGAGATACTTAAGGAGCTTGACATACCAAAGAGCATGCTTC
CAAAGCCTATGCCGTCAAGCTGTGTGTATGGTGAGGTGAATCCGGTGTACTTCGGGGGACCGATTCCTATCGCGGGAGCG
GCAGGAGACCAGCAGGCAGCGCTTTTTGGTCAGACCTGCTTTAGGGCAGGAGAAGCCAAAAATACATACGGTACAGGTTG
TTTTCTGCTGATGAATACAGGTGAGATGCCTGTTTCATCAAAGAACGGACTTGTTACCACAATAGCCTGGGGCATAGACG
GCAAGGTGGTATATGCGCTGGAGGGCTCAATATTTGTGGCAGGAGCCTCAATACAGTGGCTCCGCGATGAGATGAAATTT
ATCGATTCCTCAACTGATTCAGAGTACATGGCGAGAAAGGTAAAGGATACAAACGGCTGTTATGTGGTGCCGGCATTTAC
AGGGCTTGGGGCACCGTACTGGGATCAGTATGCGAGAGGAACTATTGTGGGTCTTACGAGAGGTGTAAACAAGTACCATG
TCATAAGGGCCACTCTGGAATCAATGGCATTTCAGGTAAATGATGTGCTTGAGGCAATGAAGGCTGATTCGGGCATCAAT
CTCACATCGCTTAAGGTGGACGGCGGCGCCAGTGCCAACAATCTGCTCATGCAGATGCAGGCTGATATCAGCAATGCACC
GGTCAATAGGCCTGTGTGTGTGGAGACAACAGCCATGGGCGCAGCGTACCTCGCAGGGCTTGCAGTGGGCTATTGGGACA
GCATGGATGATATAAAGAGAAACTGGGCTATTGACCGTGTGTTTGAGCCGGAGATAGCGGATGATATGAGAGAAAAGAAG
CGTAAAATGTGGAAAAAGGCTGTCGCCTGCGCATTTAACTGGGCGAAGGATGACTGA

Upstream 100 bases:

>100_bases
ATCGAAGTCTGTGAGTACTGATTTTATGGAAATAGCTTAAATATCATGCTATAATAATAGTAGTATTTAGGTACCGAATA
ATTGGTTGGAGGATTTTTAC

Downstream 100 bases:

>100_bases
AAGTAAAAATATATATGGAAATTGGTAGGAGAAAATAACATGTATGATGTAGTAATTATAGGAGCGGGAGTATCGGGAAG
CGCCTGTGCAAGAGAGCTAT

Product: glycerol kinase

Products: NA

Alternate protein names: ATP:glycerol 3-phosphotransferase; Glycerokinase; GK

Number of amino acids: Translated: 498; Mature: 497

Protein sequence:

>498_residues
MAKYIMALDAGTTSNRCILFNEKGEMCSVAQKEFTQFFPKPGWVEHDAEEIWATQLEVAKEAMANIQATAADICAIGITN
QRETTIVWDKNTGEPVYHAIVWQCRRTAEYADSLKEKGLTETFRKKTGLVIDAYFSATKLKWLLDNVPGARERAERGELL
FGTVETWLIWKLTQGQVHVTDYSNASRTMMFNINTLKWDDEILKELDIPKSMLPKPMPSSCVYGEVNPVYFGGPIPIAGA
AGDQQAALFGQTCFRAGEAKNTYGTGCFLLMNTGEMPVSSKNGLVTTIAWGIDGKVVYALEGSIFVAGASIQWLRDEMKF
IDSSTDSEYMARKVKDTNGCYVVPAFTGLGAPYWDQYARGTIVGLTRGVNKYHVIRATLESMAFQVNDVLEAMKADSGIN
LTSLKVDGGASANNLLMQMQADISNAPVNRPVCVETTAMGAAYLAGLAVGYWDSMDDIKRNWAIDRVFEPEIADDMREKK
RKMWKKAVACAFNWAKDD

Sequences:

>Translated_498_residues
MAKYIMALDAGTTSNRCILFNEKGEMCSVAQKEFTQFFPKPGWVEHDAEEIWATQLEVAKEAMANIQATAADICAIGITN
QRETTIVWDKNTGEPVYHAIVWQCRRTAEYADSLKEKGLTETFRKKTGLVIDAYFSATKLKWLLDNVPGARERAERGELL
FGTVETWLIWKLTQGQVHVTDYSNASRTMMFNINTLKWDDEILKELDIPKSMLPKPMPSSCVYGEVNPVYFGGPIPIAGA
AGDQQAALFGQTCFRAGEAKNTYGTGCFLLMNTGEMPVSSKNGLVTTIAWGIDGKVVYALEGSIFVAGASIQWLRDEMKF
IDSSTDSEYMARKVKDTNGCYVVPAFTGLGAPYWDQYARGTIVGLTRGVNKYHVIRATLESMAFQVNDVLEAMKADSGIN
LTSLKVDGGASANNLLMQMQADISNAPVNRPVCVETTAMGAAYLAGLAVGYWDSMDDIKRNWAIDRVFEPEIADDMREKK
RKMWKKAVACAFNWAKDD
>Mature_497_residues
AKYIMALDAGTTSNRCILFNEKGEMCSVAQKEFTQFFPKPGWVEHDAEEIWATQLEVAKEAMANIQATAADICAIGITNQ
RETTIVWDKNTGEPVYHAIVWQCRRTAEYADSLKEKGLTETFRKKTGLVIDAYFSATKLKWLLDNVPGARERAERGELLF
GTVETWLIWKLTQGQVHVTDYSNASRTMMFNINTLKWDDEILKELDIPKSMLPKPMPSSCVYGEVNPVYFGGPIPIAGAA
GDQQAALFGQTCFRAGEAKNTYGTGCFLLMNTGEMPVSSKNGLVTTIAWGIDGKVVYALEGSIFVAGASIQWLRDEMKFI
DSSTDSEYMARKVKDTNGCYVVPAFTGLGAPYWDQYARGTIVGLTRGVNKYHVIRATLESMAFQVNDVLEAMKADSGINL
TSLKVDGGASANNLLMQMQADISNAPVNRPVCVETTAMGAAYLAGLAVGYWDSMDDIKRNWAIDRVFEPEIADDMREKKR
KMWKKAVACAFNWAKDD

Specific function: Key enzyme in the regulation of glycerol uptake and metabolism

COG id: COG0554

COG function: function code C; Glycerol kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FGGY kinase family

Homologues:

Organism=Homo sapiens, GI189409120, Length=508, Percent_Identity=46.0629921259843, Blast_Score=428, Evalue=1e-120,
Organism=Homo sapiens, GI4504007, Length=507, Percent_Identity=46.1538461538462, Blast_Score=428, Evalue=1e-120,
Organism=Homo sapiens, GI41393575, Length=501, Percent_Identity=46.3073852295409, Blast_Score=427, Evalue=1e-119,
Organism=Homo sapiens, GI42794763, Length=512, Percent_Identity=45.8984375, Blast_Score=425, Evalue=1e-119,
Organism=Homo sapiens, GI88196792, Length=507, Percent_Identity=34.1222879684418, Blast_Score=322, Evalue=7e-88,
Organism=Escherichia coli, GI1790361, Length=497, Percent_Identity=63.7826961770624, Blast_Score=657, Evalue=0.0,
Organism=Escherichia coli, GI1787789, Length=517, Percent_Identity=27.2727272727273, Blast_Score=116, Evalue=4e-27,
Organism=Escherichia coli, GI1790005, Length=516, Percent_Identity=23.062015503876, Blast_Score=98, Evalue=1e-21,
Organism=Escherichia coli, GI1789987, Length=498, Percent_Identity=23.2931726907631, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1789168, Length=495, Percent_Identity=22.8282828282828, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1789138, Length=490, Percent_Identity=20.6122448979592, Blast_Score=81, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17535599, Length=503, Percent_Identity=42.7435387673956, Blast_Score=392, Evalue=1e-109,
Organism=Caenorhabditis elegans, GI71986140, Length=514, Percent_Identity=25.6809338521401, Blast_Score=181, Evalue=9e-46,
Organism=Caenorhabditis elegans, GI71986147, Length=268, Percent_Identity=30.2238805970149, Blast_Score=128, Evalue=7e-30,
Organism=Saccharomyces cerevisiae, GI6321755, Length=562, Percent_Identity=35.5871886120996, Blast_Score=306, Evalue=7e-84,
Organism=Drosophila melanogaster, GI17864214, Length=505, Percent_Identity=43.1683168316832, Blast_Score=389, Evalue=1e-108,
Organism=Drosophila melanogaster, GI24655555, Length=476, Percent_Identity=40.9663865546218, Blast_Score=368, Evalue=1e-102,
Organism=Drosophila melanogaster, GI24655560, Length=476, Percent_Identity=40.9663865546218, Blast_Score=368, Evalue=1e-102,
Organism=Drosophila melanogaster, GI24655570, Length=476, Percent_Identity=40.9663865546218, Blast_Score=368, Evalue=1e-102,
Organism=Drosophila melanogaster, GI24655574, Length=476, Percent_Identity=40.9663865546218, Blast_Score=368, Evalue=1e-102,
Organism=Drosophila melanogaster, GI24655565, Length=476, Percent_Identity=40.9663865546218, Blast_Score=368, Evalue=1e-102,
Organism=Drosophila melanogaster, GI24654573, Length=433, Percent_Identity=45.0346420323326, Blast_Score=364, Evalue=1e-101,
Organism=Drosophila melanogaster, GI19922058, Length=528, Percent_Identity=31.8181818181818, Blast_Score=265, Evalue=5e-71,
Organism=Drosophila melanogaster, GI24652871, Length=495, Percent_Identity=32.5252525252525, Blast_Score=254, Evalue=1e-67,
Organism=Drosophila melanogaster, GI24656367, Length=513, Percent_Identity=30.6042884990253, Blast_Score=242, Evalue=4e-64,
Organism=Drosophila melanogaster, GI21357867, Length=529, Percent_Identity=29.6786389413989, Blast_Score=239, Evalue=3e-63,
Organism=Drosophila melanogaster, GI24656372, Length=361, Percent_Identity=31.3019390581717, Blast_Score=187, Evalue=1e-47,
Organism=Drosophila melanogaster, GI45552909, Length=289, Percent_Identity=29.0657439446367, Blast_Score=129, Evalue=3e-30,

Paralogues:

None

Copy number: 2,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): GLPK_EUBR3 (C4ZGB4)

Other databases:

- EMBL:   CP001107
- RefSeq:   YP_002938454.1
- ProteinModelPortal:   C4ZGB4
- GeneID:   7966374
- GenomeReviews:   CP001107_GR
- KEGG:   ere:EUBREC_2589
- ProtClustDB:   CLSK2548484
- HAMAP:   MF_00186
- InterPro:   IPR000577
- InterPro:   IPR018485
- InterPro:   IPR018483
- InterPro:   IPR018484
- InterPro:   IPR005999
- PANTHER:   PTHR10196
- PANTHER:   PTHR10196:SF9
- TIGRFAMs:   TIGR01311

Pfam domain/function: PF02782 FGGY_C; PF00370 FGGY_N

EC number: =2.7.1.30

Molecular weight: Translated: 55215; Mature: 55083

Theoretical pI: Translated: 5.15; Mature: 5.15

Prosite motif: PS00933 FGGY_KINASES_1; PS00445 FGGY_KINASES_2

Important sites: BINDING 12-12 BINDING 16-16 BINDING 82-82 BINDING 134-134 BINDING 243-243 BINDING 265-265 BINDING 308-308

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKYIMALDAGTTSNRCILFNEKGEMCSVAQKEFTQFFPKPGWVEHDAEEIWATQLEVAK
CCCEEEEEECCCCCCEEEEECCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHH
EAMANIQATAADICAIGITNQRETTIVWDKNTGEPVYHAIVWQCRRTAEYADSLKEKGLT
HHHHHHHHHHHHEEEEECCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCH
ETFRKKTGLVIDAYFSATKLKWLLDNVPGARERAERGELLFGTVETWLIWKLTQGQVHVT
HHHHHHCCEEEEEECCHHHHHHHHHCCCCHHHHHHCCCEEEEEECEEEEEEEECCEEEEE
DYSNASRTMMFNINTLKWDDEILKELDIPKSMLPKPMPSSCVYGEVNPVYFGGPIPIAGA
ECCCCCEEEEEEEEEECCCHHHHHHCCCCHHHCCCCCCCCCCCCCCCEEEECCCCCCCCC
AGDQQAALFGQTCFRAGEAKNTYGTGCFLLMNTGEMPVSSKNGLVTTIAWGIDGKVVYAL
CCCCHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCCCCCCCEEEEEEECCCCEEEEEE
EGSIFVAGASIQWLRDEMKFIDSSTDSEYMARKVKDTNGCYVVPAFTGLGAPYWDQYARG
ECCEEEECCCHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCEEEECHHCCCCCHHHHHHCC
TIVGLTRGVNKYHVIRATLESMAFQVNDVLEAMKADSGINLTSLKVDGGASANNLLMQMQ
EEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCEEEEEE
ADISNAPVNRPVCVETTAMGAAYLAGLAVGYWDSMDDIKRNWAIDRVFEPEIADDMREKK
CCCCCCCCCCCEEEEHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCEECCHHHHHHHHHH
RKMWKKAVACAFNWAKDD
HHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
AKYIMALDAGTTSNRCILFNEKGEMCSVAQKEFTQFFPKPGWVEHDAEEIWATQLEVAK
CCEEEEEECCCCCCEEEEECCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHH
EAMANIQATAADICAIGITNQRETTIVWDKNTGEPVYHAIVWQCRRTAEYADSLKEKGLT
HHHHHHHHHHHHEEEEECCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCH
ETFRKKTGLVIDAYFSATKLKWLLDNVPGARERAERGELLFGTVETWLIWKLTQGQVHVT
HHHHHHCCEEEEEECCHHHHHHHHHCCCCHHHHHHCCCEEEEEECEEEEEEEECCEEEEE
DYSNASRTMMFNINTLKWDDEILKELDIPKSMLPKPMPSSCVYGEVNPVYFGGPIPIAGA
ECCCCCEEEEEEEEEECCCHHHHHHCCCCHHHCCCCCCCCCCCCCCCEEEECCCCCCCCC
AGDQQAALFGQTCFRAGEAKNTYGTGCFLLMNTGEMPVSSKNGLVTTIAWGIDGKVVYAL
CCCCHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCCCCCCCEEEEEEECCCCEEEEEE
EGSIFVAGASIQWLRDEMKFIDSSTDSEYMARKVKDTNGCYVVPAFTGLGAPYWDQYARG
ECCEEEECCCHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCEEEECHHCCCCCHHHHHHCC
TIVGLTRGVNKYHVIRATLESMAFQVNDVLEAMKADSGINLTSLKVDGGASANNLLMQMQ
EEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCEEEEEE
ADISNAPVNRPVCVETTAMGAAYLAGLAVGYWDSMDDIKRNWAIDRVFEPEIADDMREKK
CCCCCCCCCCCEEEEHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCEECCHHHHHHHHHH
RKMWKKAVACAFNWAKDD
HHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA