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The map label for this gene is truA [H]

Identifier: 238923689

GI number: 238923689

Start: 1188242

End: 1189072

Strand: Direct

Name: truA [H]

Synonym: EUBREC_1311

Alternate gene names: 238923689

Gene position: 1188242-1189072 (Clockwise)

Preceding gene: 238923688

Following gene: 238923690

Centisome position: 34.44

GC content: 43.08

Gene sequence:

>831_bases
GTGATATTATATAAAATAAAAGTAACTATTCACAACCCTATTTTTCAGATAAAGAAAGACAATTTTATGAATACAGAAAC
CCAAAATTACAAGATAATTGTGGCATACGACGGCACACGCTACAAAGGCTGGCAGGTGCAGAAATCCACAGATGATACAA
TACAGGGAAAGCTTCAGCATGTGCTTTCCACACTGGCAGGAAAGCCGGTTGAGGTCATAGGCTCAGGGCGCACGGATGCG
GGAGTGCATGCGGTGGGGCAGGTGGCAAGCTTTCACATGCCGAAGCATTTTTCAAAGGATGAGATATTTATATGGCTGAA
TGAGCATCTGCCGGCAGATATTGCGGTGACAGATATTTCAAATGTGCCTGACAGATTTCATGCCAGGTACAATGCCGTCT
CAAAGACATATGTGTATACAATACACACCGGTATTGTGTCAGATGTGTTCAGGAGAAAATATGTATACGACTATGATAAG
CCGCTTGACACAGACCGGATGAAAAAAGCAGCAGCGTATCTTCTCGGAGAGCATGATTTTAAAGCCTTTTGTGGCAATAG
ACATATGAAAAAGTCAACGGTCAGGACGATTTTTTCTATTGATATAGAAAAAACAGGCGCTGATATTGTTATCAGCTACA
CCGGAGATGGATTTTTACAGAATATGATAAGAATCATCACAGGAACGCTTATCGAGGTGGGCGATGGAAGGAAAAATCCG
GATGCTGTGATGGAAATTTTAGCTTCAAAGGATAGGGCACAGGCAGGCTACACAGCACCGGCGTGTGGGCTGAGACTGGA
AAGGGTAGATTATGGCAAAGTGGTATGTTAG

Upstream 100 bases:

>100_bases
GGTATGAGATATCAAATATATATGTCGCTGTGACACGTATTGCGGAGCTTGCTTAATTGGATTAAAGGAGCAAAACAGTG
CTGGCAAGAGAAAAAATGAT

Downstream 100 bases:

>100_bases
TGCGAAGAGGGCTGATTTTGCGGCAATCGGAGCGAAGTTTGGAATAGATCAGGTGACGGCGCGGATAATCAGAAACAGAG
GTGTTATAGGAGATGACGCG

Product: tRNA pseudouridine synthase A

Products: NA

Alternate protein names: tRNA pseudouridylate synthase I; tRNA-uridine isomerase I [H]

Number of amino acids: Translated: 276; Mature: 276

Protein sequence:

>276_residues
MILYKIKVTIHNPIFQIKKDNFMNTETQNYKIIVAYDGTRYKGWQVQKSTDDTIQGKLQHVLSTLAGKPVEVIGSGRTDA
GVHAVGQVASFHMPKHFSKDEIFIWLNEHLPADIAVTDISNVPDRFHARYNAVSKTYVYTIHTGIVSDVFRRKYVYDYDK
PLDTDRMKKAAAYLLGEHDFKAFCGNRHMKKSTVRTIFSIDIEKTGADIVISYTGDGFLQNMIRIITGTLIEVGDGRKNP
DAVMEILASKDRAQAGYTAPACGLRLERVDYGKVVC

Sequences:

>Translated_276_residues
MILYKIKVTIHNPIFQIKKDNFMNTETQNYKIIVAYDGTRYKGWQVQKSTDDTIQGKLQHVLSTLAGKPVEVIGSGRTDA
GVHAVGQVASFHMPKHFSKDEIFIWLNEHLPADIAVTDISNVPDRFHARYNAVSKTYVYTIHTGIVSDVFRRKYVYDYDK
PLDTDRMKKAAAYLLGEHDFKAFCGNRHMKKSTVRTIFSIDIEKTGADIVISYTGDGFLQNMIRIITGTLIEVGDGRKNP
DAVMEILASKDRAQAGYTAPACGLRLERVDYGKVVC
>Mature_276_residues
MILYKIKVTIHNPIFQIKKDNFMNTETQNYKIIVAYDGTRYKGWQVQKSTDDTIQGKLQHVLSTLAGKPVEVIGSGRTDA
GVHAVGQVASFHMPKHFSKDEIFIWLNEHLPADIAVTDISNVPDRFHARYNAVSKTYVYTIHTGIVSDVFRRKYVYDYDK
PLDTDRMKKAAAYLLGEHDFKAFCGNRHMKKSTVRTIFSIDIEKTGADIVISYTGDGFLQNMIRIITGTLIEVGDGRKNP
DAVMEILASKDRAQAGYTAPACGLRLERVDYGKVVC

Specific function: Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs [H]

COG id: COG0101

COG function: function code J; Pseudouridylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA pseudouridine synthase truA family [H]

Homologues:

Organism=Homo sapiens, GI23503259, Length=275, Percent_Identity=32, Blast_Score=101, Evalue=9e-22,
Organism=Homo sapiens, GI199559785, Length=252, Percent_Identity=26.5873015873016, Blast_Score=69, Evalue=4e-12,
Organism=Escherichia coli, GI1788657, Length=245, Percent_Identity=39.1836734693878, Blast_Score=171, Evalue=3e-44,
Organism=Caenorhabditis elegans, GI71985046, Length=304, Percent_Identity=23.6842105263158, Blast_Score=73, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6321112, Length=253, Percent_Identity=28.0632411067194, Blast_Score=79, Evalue=5e-16,
Organism=Drosophila melanogaster, GI116007752, Length=300, Percent_Identity=26.6666666666667, Blast_Score=76, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR001406
- InterPro:   IPR020097
- InterPro:   IPR020095
- InterPro:   IPR020094 [H]

Pfam domain/function: PF01416 PseudoU_synth_1 [H]

EC number: =5.4.99.12 [H]

Molecular weight: Translated: 31116; Mature: 31116

Theoretical pI: Translated: 9.29; Mature: 9.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MILYKIKVTIHNPIFQIKKDNFMNTETQNYKIIVAYDGTRYKGWQVQKSTDDTIQGKLQH
CEEEEEEEEEECCEEEEECCCCCCCCCCCEEEEEEECCCCCCCEEEECCCCHHHHHHHHH
VLSTLAGKPVEVIGSGRTDAGVHAVGQVASFHMPKHFSKDEIFIWLNEHLPADIAVTDIS
HHHHHCCCCEEEEECCCCCCHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCEEEECCC
NVPDRFHARYNAVSKTYVYTIHTGIVSDVFRRKYVYDYDKPLDTDRMKKAAAYLLGEHDF
CCCHHHHHHHHCCCEEEEEEEEHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCH
KAFCGNRHMKKSTVRTIFSIDIEKTGADIVISYTGDGFLQNMIRIITGTLIEVGDGRKNP
HHHHCCCCCHHHHEEEEEEEEEECCCCEEEEEECCCHHHHHHHHHHHHHEEEECCCCCCH
DAVMEILASKDRAQAGYTAPACGLRLERVDYGKVVC
HHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCCCC
>Mature Secondary Structure
MILYKIKVTIHNPIFQIKKDNFMNTETQNYKIIVAYDGTRYKGWQVQKSTDDTIQGKLQH
CEEEEEEEEEECCEEEEECCCCCCCCCCCEEEEEEECCCCCCCEEEECCCCHHHHHHHHH
VLSTLAGKPVEVIGSGRTDAGVHAVGQVASFHMPKHFSKDEIFIWLNEHLPADIAVTDIS
HHHHHCCCCEEEEECCCCCCHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCEEEECCC
NVPDRFHARYNAVSKTYVYTIHTGIVSDVFRRKYVYDYDKPLDTDRMKKAAAYLLGEHDF
CCCHHHHHHHHCCCEEEEEEEEHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCH
KAFCGNRHMKKSTVRTIFSIDIEKTGADIVISYTGDGFLQNMIRIITGTLIEVGDGRKNP
HHHHCCCCCHHHHEEEEEEEEEECCCCEEEEEECCCHHHHHHHHHHHHHEEEECCCCCCH
DAVMEILASKDRAQAGYTAPACGLRLERVDYGKVVC
HHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA