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The map label for this gene is ilvB [H]

Identifier: 238923628

GI number: 238923628

Start: 1146751

End: 1148472

Strand: Direct

Name: ilvB [H]

Synonym: EUBREC_1250

Alternate gene names: 238923628

Gene position: 1146751-1148472 (Clockwise)

Preceding gene: 238923627

Following gene: 238923629

Centisome position: 33.24

GC content: 45.7

Gene sequence:

>1722_bases
ATGGACATCACAGGAAGAAAATTATTTGTAAAAGCATTGGAGGAGGAAGGCGTAGATACTATTTTTGCCTATCCGGGAGG
AACAGTTACAGATTTATTCGATGAGTTATACAAGACTAATTCAATCAATCTGGTTCTGCCGAGACATGAGCAGGGCCTGA
TTCATGAGGCTGAGGGCTATGCAAAGTCTACAGGCAAGGTGGGTGTCTGCCTTGTGACAAGCGGTCCCGGTGCTACCAAC
ACAGTTACAGGTCTTGCAGATGCGCATTATGACAATGTGCCACTTGTGTGCTTTACAGGGCAGGTACCGCTTCCACTTAT
CGGAAATGATGCCTTTCAGGAAGTGGATATAGTCGGAATCACCAGAAATATCACAAAGTATTCTGTTACAGTCCGTGACA
GAAAGGACCTTGGAAAAATCATAAAGATGGCATTTCATATTGCAAGGACCGGAAAGCCGGGCCCTGTACTGATTGATTTG
CCAAAGGATATACAGACTGCATCCGGTCCGGCAGAGTATCCGGACAAGGTGGAAATCAGAGGCTATCGTGTAAATGATAC
AGTACATGTCGGACAGCTTAAGCGCGCCTACAAGATGTTAAAGAGTGCAAAAAAACCGCTTATCCTTGCAGGAGGCGGTA
TAAATGTTGCCAGGGCAAATGAAAACCTGCGCCGTTTTGCGGAGGCAGAGAATGTGCCTGTTGTCACTACAATAATGGGT
AAGGGAGCTATACCTACCACCCATCCGCTTTATGTGGGAAACTGTGGTATGCACGGCAAATATGCGGCAAACAAGGCAGT
CAGTGAGTGTGATTTACTTTTTTCAATAGGTACACGATTTAATGACAGAATAACCGGTGACTTAAATGAGTTCGCTCCAA
AGGCAAAGATTGTACATATTGATGTGGATACTGCATCTATTTCACGAAATGTAGTGGTTGATGTGCCTATCGTTGCAGAT
GCCAATGTGGCGCTCGACAAGTTAAACGAGTGGGCTGAGCCGATAAAGACTGCTGAGTGGCAGAAGCAGATTAAGGCATG
GGATGAGGAAAATCCTCTTGGCATGAGAAGAGACAAGGGTATGACACCTCAGATGATTATGGAGCATATCAATAAGAACT
ATAAGGATGCAATCTACGTTACAGATGTAGGTCAGCATCAGATGTGGGCTACACAGTACTTAGAGCTTGACAGCAACAGC
CAGCTTCTTACATCGGGAGGTCTTGGAACCATGGGCTTTGGTCTTCCGGCAGCAATAGGTGCAAAGATTGCAAATCCTGA
CAAGGATGTTGTATGCATAAGCGGTGACGGTGGACTTCAGATGAATATACAGGAGCTTGCCACCTCAGTTGTACAGGGAG
CAGGAGTGACCATATGCGTGCTCAATAACTACTATCTGGGCATGGTGCGCCAGATGCAGCAGCTTTTTTATGGCAAGCGT
TATTCTGCAACTTGCCTTCGAAGAAGAAAGGGCTGCCCGGATGAGTGCAAGGGACCAAACGAGGCATGTCCGCCATACAC
ACCGGATTTTCTCAAGCTTGTTGAAAGCTATGGTGGACACGGAATCCGCGTGGAAAAGGAAGAGGATATCGATGCAGCCT
TTGCAGAGGCAAAGAAATATAAGGATGTACCTGTAATTATTGAGTTTATGATAGCTACGGATGAGATTGTTCTTCCAATG
GTCAAGAGCGGAAATCCTATGAGCGAAATGATTTTAAAGTAG

Upstream 100 bases:

>100_bases
GGAGACAGCGAAGAGCAGACATACTTTGATTTGGAGTTTTATTTCCTTCGATATGAGAAAGTAAAATAAACTATTAAATA
GAAACGAGGATGACAGCAGT

Downstream 100 bases:

>100_bases
GAGGTGCAGAAGATGAATAATATTGAAATGAAAAACAGATGGATTGCACTTTATGTTGAGAATGAGGTCGGTGTTTTAGC
AAAGGTTTCAGGACTTTTCT

Product: acetolactate synthase, large subunit, biosynthetic type

Products: NA

Alternate protein names: AHAS; Acetohydroxy-acid synthase large subunit; ALS; Vegetative protein 105; VEG105 [H]

Number of amino acids: Translated: 573; Mature: 573

Protein sequence:

>573_residues
MDITGRKLFVKALEEEGVDTIFAYPGGTVTDLFDELYKTNSINLVLPRHEQGLIHEAEGYAKSTGKVGVCLVTSGPGATN
TVTGLADAHYDNVPLVCFTGQVPLPLIGNDAFQEVDIVGITRNITKYSVTVRDRKDLGKIIKMAFHIARTGKPGPVLIDL
PKDIQTASGPAEYPDKVEIRGYRVNDTVHVGQLKRAYKMLKSAKKPLILAGGGINVARANENLRRFAEAENVPVVTTIMG
KGAIPTTHPLYVGNCGMHGKYAANKAVSECDLLFSIGTRFNDRITGDLNEFAPKAKIVHIDVDTASISRNVVVDVPIVAD
ANVALDKLNEWAEPIKTAEWQKQIKAWDEENPLGMRRDKGMTPQMIMEHINKNYKDAIYVTDVGQHQMWATQYLELDSNS
QLLTSGGLGTMGFGLPAAIGAKIANPDKDVVCISGDGGLQMNIQELATSVVQGAGVTICVLNNYYLGMVRQMQQLFYGKR
YSATCLRRRKGCPDECKGPNEACPPYTPDFLKLVESYGGHGIRVEKEEDIDAAFAEAKKYKDVPVIIEFMIATDEIVLPM
VKSGNPMSEMILK

Sequences:

>Translated_573_residues
MDITGRKLFVKALEEEGVDTIFAYPGGTVTDLFDELYKTNSINLVLPRHEQGLIHEAEGYAKSTGKVGVCLVTSGPGATN
TVTGLADAHYDNVPLVCFTGQVPLPLIGNDAFQEVDIVGITRNITKYSVTVRDRKDLGKIIKMAFHIARTGKPGPVLIDL
PKDIQTASGPAEYPDKVEIRGYRVNDTVHVGQLKRAYKMLKSAKKPLILAGGGINVARANENLRRFAEAENVPVVTTIMG
KGAIPTTHPLYVGNCGMHGKYAANKAVSECDLLFSIGTRFNDRITGDLNEFAPKAKIVHIDVDTASISRNVVVDVPIVAD
ANVALDKLNEWAEPIKTAEWQKQIKAWDEENPLGMRRDKGMTPQMIMEHINKNYKDAIYVTDVGQHQMWATQYLELDSNS
QLLTSGGLGTMGFGLPAAIGAKIANPDKDVVCISGDGGLQMNIQELATSVVQGAGVTICVLNNYYLGMVRQMQQLFYGKR
YSATCLRRRKGCPDECKGPNEACPPYTPDFLKLVESYGGHGIRVEKEEDIDAAFAEAKKYKDVPVIIEFMIATDEIVLPM
VKSGNPMSEMILK
>Mature_573_residues
MDITGRKLFVKALEEEGVDTIFAYPGGTVTDLFDELYKTNSINLVLPRHEQGLIHEAEGYAKSTGKVGVCLVTSGPGATN
TVTGLADAHYDNVPLVCFTGQVPLPLIGNDAFQEVDIVGITRNITKYSVTVRDRKDLGKIIKMAFHIARTGKPGPVLIDL
PKDIQTASGPAEYPDKVEIRGYRVNDTVHVGQLKRAYKMLKSAKKPLILAGGGINVARANENLRRFAEAENVPVVTTIMG
KGAIPTTHPLYVGNCGMHGKYAANKAVSECDLLFSIGTRFNDRITGDLNEFAPKAKIVHIDVDTASISRNVVVDVPIVAD
ANVALDKLNEWAEPIKTAEWQKQIKAWDEENPLGMRRDKGMTPQMIMEHINKNYKDAIYVTDVGQHQMWATQYLELDSNS
QLLTSGGLGTMGFGLPAAIGAKIANPDKDVVCISGDGGLQMNIQELATSVVQGAGVTICVLNNYYLGMVRQMQQLFYGKR
YSATCLRRRKGCPDECKGPNEACPPYTPDFLKLVESYGGHGIRVEKEEDIDAAFAEAKKYKDVPVIIEFMIATDEIVLPM
VKSGNPMSEMILK

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=573, Percent_Identity=24.6073298429319, Blast_Score=153, Evalue=6e-37,
Organism=Homo sapiens, GI21361361, Length=564, Percent_Identity=24.645390070922, Blast_Score=142, Evalue=9e-34,
Organism=Escherichia coli, GI87081685, Length=584, Percent_Identity=41.6095890410959, Blast_Score=471, Evalue=1e-134,
Organism=Escherichia coli, GI1790104, Length=573, Percent_Identity=39.4415357766143, Blast_Score=394, Evalue=1e-111,
Organism=Escherichia coli, GI1786717, Length=556, Percent_Identity=36.3309352517986, Blast_Score=323, Evalue=1e-89,
Organism=Escherichia coli, GI1787096, Length=571, Percent_Identity=27.6707530647986, Blast_Score=188, Evalue=1e-48,
Organism=Escherichia coli, GI1788716, Length=479, Percent_Identity=25.0521920668058, Blast_Score=135, Evalue=7e-33,
Organism=Caenorhabditis elegans, GI17531299, Length=576, Percent_Identity=26.5625, Blast_Score=157, Evalue=1e-38,
Organism=Caenorhabditis elegans, GI17531301, Length=576, Percent_Identity=26.5625, Blast_Score=157, Evalue=2e-38,
Organism=Caenorhabditis elegans, GI17542570, Length=502, Percent_Identity=25.0996015936255, Blast_Score=128, Evalue=6e-30,
Organism=Saccharomyces cerevisiae, GI6323755, Length=594, Percent_Identity=39.8989898989899, Blast_Score=419, Evalue=1e-118,
Organism=Saccharomyces cerevisiae, GI6320816, Length=486, Percent_Identity=24.0740740740741, Blast_Score=110, Evalue=7e-25,
Organism=Saccharomyces cerevisiae, GI6323163, Length=484, Percent_Identity=23.7603305785124, Blast_Score=100, Evalue=4e-22,
Organism=Saccharomyces cerevisiae, GI6321524, Length=483, Percent_Identity=21.1180124223602, Blast_Score=90, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6323073, Length=509, Percent_Identity=23.3791748526523, Blast_Score=89, Evalue=3e-18,
Organism=Drosophila melanogaster, GI19922626, Length=483, Percent_Identity=26.2939958592132, Blast_Score=154, Evalue=2e-37,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 62666; Mature: 62666

Theoretical pI: Translated: 6.59; Mature: 6.59

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDITGRKLFVKALEEEGVDTIFAYPGGTVTDLFDELYKTNSINLVLPRHEQGLIHEAEGY
CCCCCHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHCCCCEEEEECCCCCCCEECCCCC
AKSTGKVGVCLVTSGPGATNTVTGLADAHYDNVPLVCFTGQVPLPLIGNDAFQEVDIVGI
CCCCCCEEEEEEECCCCCCCCEECCCCCCCCCCCEEEEECCCCCCEECCCCCCEEEEEEE
TRNITKYSVTVRDRKDLGKIIKMAFHIARTGKPGPVLIDLPKDIQTASGPAEYPDKVEIR
EECCEEEEEEECCHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCCCCCCCCEEEE
GYRVNDTVHVGQLKRAYKMLKSAKKPLILAGGGINVARANENLRRFAEAENVPVVTTIMG
EEEECCEEEHHHHHHHHHHHHHCCCCEEEECCCEEEEECCHHHHHHHHHCCCCEEEEEEC
KGAIPTTHPLYVGNCGMHGKYAANKAVSECDLLFSIGTRFNDRITGDLNEFAPKAKIVHI
CCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHCCCCEEEEEE
DVDTASISRNVVVDVPIVADANVALDKLNEWAEPIKTAEWQKQIKAWDEENPLGMRRDKG
ECCHHHCCCCEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
MTPQMIMEHINKNYKDAIYVTDVGQHQMWATQYLELDSNSQLLTSGGLGTMGFGLPAAIG
CCHHHHHHHHCCCCCCEEEEEECCCCHHEEEHEEEECCCCEEEECCCCCCCCCCCHHHHC
AKIANPDKDVVCISGDGGLQMNIQELATSVVQGAGVTICVLNNYYLGMVRQMQQLFYGKR
CCCCCCCCCEEEEECCCCCEEEHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHCCCC
YSATCLRRRKGCPDECKGPNEACPPYTPDFLKLVESYGGHGIRVEKEEDIDAAFAEAKKY
CHHHHHHHHCCCCHHHCCCCCCCCCCCHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHHC
KDVPVIIEFMIATDEIVLPMVKSGNPMSEMILK
CCCCEEEEEEEECCCEEEEHCCCCCCHHHHHCC
>Mature Secondary Structure
MDITGRKLFVKALEEEGVDTIFAYPGGTVTDLFDELYKTNSINLVLPRHEQGLIHEAEGY
CCCCCHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHCCCCEEEEECCCCCCCEECCCCC
AKSTGKVGVCLVTSGPGATNTVTGLADAHYDNVPLVCFTGQVPLPLIGNDAFQEVDIVGI
CCCCCCEEEEEEECCCCCCCCEECCCCCCCCCCCEEEEECCCCCCEECCCCCCEEEEEEE
TRNITKYSVTVRDRKDLGKIIKMAFHIARTGKPGPVLIDLPKDIQTASGPAEYPDKVEIR
EECCEEEEEEECCHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCCCCCCCCEEEE
GYRVNDTVHVGQLKRAYKMLKSAKKPLILAGGGINVARANENLRRFAEAENVPVVTTIMG
EEEECCEEEHHHHHHHHHHHHHCCCCEEEECCCEEEEECCHHHHHHHHHCCCCEEEEEEC
KGAIPTTHPLYVGNCGMHGKYAANKAVSECDLLFSIGTRFNDRITGDLNEFAPKAKIVHI
CCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHCCCCEEEEEE
DVDTASISRNVVVDVPIVADANVALDKLNEWAEPIKTAEWQKQIKAWDEENPLGMRRDKG
ECCHHHCCCCEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
MTPQMIMEHINKNYKDAIYVTDVGQHQMWATQYLELDSNSQLLTSGGLGTMGFGLPAAIG
CCHHHHHHHHCCCCCCEEEEEECCCCHHEEEHEEEECCCCEEEECCCCCCCCCCCHHHHC
AKIANPDKDVVCISGDGGLQMNIQELATSVVQGAGVTICVLNNYYLGMVRQMQQLFYGKR
CCCCCCCCCEEEEECCCCCEEEHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHCCCC
YSATCLRRRKGCPDECKGPNEACPPYTPDFLKLVESYGGHGIRVEKEEDIDAAFAEAKKY
CHHHHHHHHCCCCHHHCCCCCCCCCCCHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHHC
KDVPVIIEFMIATDEIVLPMVKSGNPMSEMILK
CCCCEEEEEEEECCCEEEEHCCCCCCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969504; 9384377; 1577690; 9298659 [H]