Click here to switch to the map view.

The map label for this gene is dfrA [H]

Identifier: 238923627

GI number: 238923627

Start: 1146228

End: 1146719

Strand: Direct

Name: dfrA [H]

Synonym: EUBREC_1249

Alternate gene names: 238923627

Gene position: 1146228-1146719 (Clockwise)

Preceding gene: 238923626

Following gene: 238923628

Centisome position: 33.23

GC content: 42.07

Gene sequence:

>492_bases
ATGAATTTAATAGCAGCAGTTGACAATAACTGGGCAATTGGAAAAAATAACCAGTTGCTCGTTCGGATACCTATGGACCA
GAAGTTTTTCCGCGAGATGACTACAGGAAAGGTTGTAGTCATGGGCAGAAAGACACTTGAGAGCTTTCCAAACAGCAGAC
CTCTTAAGAACAGAACCAACATAGTGCTCACACATAACCCATCCTATGAGGTGGAGGGTGCTGTCGTGGTTCACTCACTT
GATGAGCTTCACAAGGAGCTTGAAAAATACAATTCTGAGGATATTTATATCATTGGTGGACAGAAAATTTATGAGCAGCT
CGTGGATGAATGCGATGTAGCCCACATCACAAAGGTGGACTTCGAGTATGATGCGGATGCATATTTTCCAAATCTTGACG
AGAAGCCTGAGTGGCAGATTACGGGAGACAGCGAAGAGCAGACATACTTTGATTTGGAGTTTTATTTCCTTCGATATGAG
AAAGTAAAATAA

Upstream 100 bases:

>100_bases
TACCTATTGCGGTTTAAAGAATGTGGATATGAAGCAGTTATAAGAAGGTTATTGTTCACTACTTTGTGTGAACAGCAACG
ATTACAACAGGGAGAAAATC

Downstream 100 bases:

>100_bases
ACTATTAAATAGAAACGAGGATGACAGCAGTATGGACATCACAGGAAGAAAATTATTTGTAAAAGCATTGGAGGAGGAAG
GCGTAGATACTATTTTTGCC

Product: dihydrofolate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 163; Mature: 163

Protein sequence:

>163_residues
MNLIAAVDNNWAIGKNNQLLVRIPMDQKFFREMTTGKVVVMGRKTLESFPNSRPLKNRTNIVLTHNPSYEVEGAVVVHSL
DELHKELEKYNSEDIYIIGGQKIYEQLVDECDVAHITKVDFEYDADAYFPNLDEKPEWQITGDSEEQTYFDLEFYFLRYE
KVK

Sequences:

>Translated_163_residues
MNLIAAVDNNWAIGKNNQLLVRIPMDQKFFREMTTGKVVVMGRKTLESFPNSRPLKNRTNIVLTHNPSYEVEGAVVVHSL
DELHKELEKYNSEDIYIIGGQKIYEQLVDECDVAHITKVDFEYDADAYFPNLDEKPEWQITGDSEEQTYFDLEFYFLRYE
KVK
>Mature_163_residues
MNLIAAVDNNWAIGKNNQLLVRIPMDQKFFREMTTGKVVVMGRKTLESFPNSRPLKNRTNIVLTHNPSYEVEGAVVVHSL
DELHKELEKYNSEDIYIIGGQKIYEQLVDECDVAHITKVDFEYDADAYFPNLDEKPEWQITGDSEEQTYFDLEFYFLRYE
KVK

Specific function: Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis [H]

COG id: COG0262

COG function: function code H; Dihydrofolate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DHFR (dihydrofolate reductase) domain [H]

Homologues:

Organism=Homo sapiens, GI28827793, Length=152, Percent_Identity=35.5263157894737, Blast_Score=70, Evalue=9e-13,
Organism=Homo sapiens, GI4503323, Length=150, Percent_Identity=34, Blast_Score=66, Evalue=1e-11,
Organism=Escherichia coli, GI1786233, Length=139, Percent_Identity=33.0935251798561, Blast_Score=77, Evalue=7e-16,
Organism=Caenorhabditis elegans, GI17505899, Length=151, Percent_Identity=37.7483443708609, Blast_Score=77, Evalue=3e-15,
Organism=Drosophila melanogaster, GI116008026, Length=146, Percent_Identity=36.986301369863, Blast_Score=76, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24647458, Length=146, Percent_Identity=36.986301369863, Blast_Score=76, Evalue=1e-14,

Paralogues:

None

Copy number: 640 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 10,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012259
- InterPro:   IPR017925
- InterPro:   IPR001796 [H]

Pfam domain/function: PF00186 DHFR_1 [H]

EC number: =1.5.1.3 [H]

Molecular weight: Translated: 19090; Mature: 19090

Theoretical pI: Translated: 4.53; Mature: 4.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLIAAVDNNWAIGKNNQLLVRIPMDQKFFREMTTGKVVVMGRKTLESFPNSRPLKNRTN
CCEEEEECCCEEECCCCEEEEEECCCHHHHHHHCCCEEEEEECHHHHHCCCCCCCCCCCC
IVLTHNPSYEVEGAVVVHSLDELHKELEKYNSEDIYIIGGQKIYEQLVDECDVAHITKVD
EEEEECCCCCCCCEEEEECHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHCCCEEEEEEC
FEYDADAYFPNLDEKPEWQITGDSEEQTYFDLEFYFLRYEKVK
CEECCCCCCCCCCCCCCEEEECCCCCCEEEEEEEEEEEEEECC
>Mature Secondary Structure
MNLIAAVDNNWAIGKNNQLLVRIPMDQKFFREMTTGKVVVMGRKTLESFPNSRPLKNRTN
CCEEEEECCCEEECCCCEEEEEECCCHHHHHHHCCCEEEEEECHHHHHCCCCCCCCCCCC
IVLTHNPSYEVEGAVVVHSLDELHKELEKYNSEDIYIIGGQKIYEQLVDECDVAHITKVD
EEEEECCCCCCCCEEEEECHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHCCCEEEEEEC
FEYDADAYFPNLDEKPEWQITGDSEEQTYFDLEFYFLRYEKVK
CEECCCCCCCCCCCCCCEEEECCCCCCEEEEEEEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2840350; 9384377 [H]