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The map label for this gene is splB [H]

Identifier: 238923622

GI number: 238923622

Start: 1142146

End: 1143147

Strand: Direct

Name: splB [H]

Synonym: EUBREC_1244

Alternate gene names: 238923622

Gene position: 1142146-1143147 (Clockwise)

Preceding gene: 238923621

Following gene: 238923623

Centisome position: 33.11

GC content: 42.91

Gene sequence:

>1002_bases
ATGTGCCAATACAATAAAATGTTTTCACATGTATATGTGGAAAAAGGCGTGGCCGAGCATCCACGTACAAAGCAGATTTT
AGAAAAGCTGAATAATACAACAGTCATAGAAATTGACCACTACAAGGATGTTTTCTGCCGCAAAAAGCAGAGTATTTCTG
CTCAAAGCAATGCCAAGGCGCTCATTCTGGCAGAGAATACAAGCGGCTGCATATATGAAGGTGCACCGGTGTGTCAGAGC
TTTGGAAATGAGAATTTCTACTATTGCTCATGCATGATGAACTGTATATTTGACTGCGAATACTGCTATTTAAAGGGCAT
GTATCCGTCTGGTAATCTTGTGGTGTTTGTAAATTTGGAGGATATTTTTGCGGAGCTTGAAGCACTTCTGGCGCAAAAAA
GCATATATCTTTGCGTGTCATATGATGCAGACCTCGTGGCCATAGAGTCGCTTACCGGCTTTGTGAGGGAATGGATTGCT
TTTACAAAAAAGCATGAGAATCTTACAATTGAAATCCGCACCAAGAGTGGCAGGTGTGATTTGTGGAGCAATTACGAAGC
CTGTGACAGGGTAATTATTGCCTATACAGTTTCGCCTCAAAGGATTGCAGCAGAGTATGAGCATTTTGCGTCCGCACCTA
TGGAGCGGATTCAGGCAGCAAAATGTGCTATGGATGGTGGATTTCCAGTGCGACTGTGCTTTGATCCGATGATATACTGC
AAGGACTGGAGAGGTGAATACAGCAGGCTGGTTGATGACGTATTTTCACAGATAGACGGTTTTACGCTGTGGGATGTGAG
TATCGGAAGCTTCCGCATATCGCAGGATTATCTGAAGAAAATGCGAAAGGACATGCCTCGTAGTGCTGTGGTGAATTTTC
CATACGACAATGTGAATGGGTATTACCAGTATCCGGAAAATATACGAAGTGATATGGAAGAGTTTATGGTACAGGCTGTA
TCCGAATATGTGGATAAGGACAGGATATTTATGTGGAAGTGA

Upstream 100 bases:

>100_bases
AGTACTATACAAATGGCTTGCTGCCGTGTGAGAGCAAGCGTGAAGGAAAGAAATGTCTGTTTGAATTGAAACAGAGATTG
TTGTAAGAAGGAAATGTCAA

Downstream 100 bases:

>100_bases
TATTATTGCAATACATTTTCAATATATATATCACATGGTATGATTAAAGGGTCAAAGTATAAAGGAGCAATCAGATGAAC
AAAGCAATTGTAACCGGGGC

Product: spore photoproduct

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 333; Mature: 333

Protein sequence:

>333_residues
MCQYNKMFSHVYVEKGVAEHPRTKQILEKLNNTTVIEIDHYKDVFCRKKQSISAQSNAKALILAENTSGCIYEGAPVCQS
FGNENFYYCSCMMNCIFDCEYCYLKGMYPSGNLVVFVNLEDIFAELEALLAQKSIYLCVSYDADLVAIESLTGFVREWIA
FTKKHENLTIEIRTKSGRCDLWSNYEACDRVIIAYTVSPQRIAAEYEHFASAPMERIQAAKCAMDGGFPVRLCFDPMIYC
KDWRGEYSRLVDDVFSQIDGFTLWDVSIGSFRISQDYLKKMRKDMPRSAVVNFPYDNVNGYYQYPENIRSDMEEFMVQAV
SEYVDKDRIFMWK

Sequences:

>Translated_333_residues
MCQYNKMFSHVYVEKGVAEHPRTKQILEKLNNTTVIEIDHYKDVFCRKKQSISAQSNAKALILAENTSGCIYEGAPVCQS
FGNENFYYCSCMMNCIFDCEYCYLKGMYPSGNLVVFVNLEDIFAELEALLAQKSIYLCVSYDADLVAIESLTGFVREWIA
FTKKHENLTIEIRTKSGRCDLWSNYEACDRVIIAYTVSPQRIAAEYEHFASAPMERIQAAKCAMDGGFPVRLCFDPMIYC
KDWRGEYSRLVDDVFSQIDGFTLWDVSIGSFRISQDYLKKMRKDMPRSAVVNFPYDNVNGYYQYPENIRSDMEEFMVQAV
SEYVDKDRIFMWK
>Mature_333_residues
MCQYNKMFSHVYVEKGVAEHPRTKQILEKLNNTTVIEIDHYKDVFCRKKQSISAQSNAKALILAENTSGCIYEGAPVCQS
FGNENFYYCSCMMNCIFDCEYCYLKGMYPSGNLVVFVNLEDIFAELEALLAQKSIYLCVSYDADLVAIESLTGFVREWIA
FTKKHENLTIEIRTKSGRCDLWSNYEACDRVIIAYTVSPQRIAAEYEHFASAPMERIQAAKCAMDGGFPVRLCFDPMIYC
KDWRGEYSRLVDDVFSQIDGFTLWDVSIGSFRISQDYLKKMRKDMPRSAVVNFPYDNVNGYYQYPENIRSDMEEFMVQAV
SEYVDKDRIFMWK

Specific function: Involved in repair of UV radiation-induced DNA damage during spore germination. Can repair thymine dimer 5-thyminyl-5,6- dihydrothymine (known as spore photoproduct (SP)) by in situ monomerization of SP to two thymines [H]

COG id: COG1533

COG function: function code L; DNA repair photolyase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. SPL family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =4.1.99.14 [H]

Molecular weight: Translated: 38619; Mature: 38619

Theoretical pI: Translated: 5.01; Mature: 5.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.5 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
8.4 %Cys+Met (Translated Protein)
4.5 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
8.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCQYNKMFSHVYVEKGVAEHPRTKQILEKLNNTTVIEIDHYKDVFCRKKQSISAQSNAKA
CCCHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCEEEEECCHHHHHHHHHHCCCCCCCCCE
LILAENTSGCIYEGAPVCQSFGNENFYYCSCMMNCIFDCEYCYLKGMYPSGNLVVFVNLE
EEEEECCCCCEECCCHHHHHCCCCCEEEHHHHHHHHHCCCCCEEEECCCCCCEEEEEEHH
DIFAELEALLAQKSIYLCVSYDADLVAIESLTGFVREWIAFTKKHENLTIEIRTKSGRCD
HHHHHHHHHHHCCCEEEEEECCCCEEHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCC
LWSNYEACDRVIIAYTVSPQRIAAEYEHFASAPMERIQAAKCAMDGGFPVRLCFDPMIYC
CCCCHHHHCEEEEEEECCHHHHHHHHHHHHHCHHHHHHHHHHHCCCCCEEEEEECCCEEE
KDWRGEYSRLVDDVFSQIDGFTLWDVSIGSFRISQDYLKKMRKDMPRSAVVNFPYDNVNG
ECCCCHHHHHHHHHHHHHCCEEEEEEECCCEEECHHHHHHHHHCCCCHHEEECCCCCCCC
YYQYPENIRSDMEEFMVQAVSEYVDKDRIFMWK
EEECCHHHHHHHHHHHHHHHHHHHCCCCEEEEC
>Mature Secondary Structure
MCQYNKMFSHVYVEKGVAEHPRTKQILEKLNNTTVIEIDHYKDVFCRKKQSISAQSNAKA
CCCHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCEEEEECCHHHHHHHHHHCCCCCCCCCE
LILAENTSGCIYEGAPVCQSFGNENFYYCSCMMNCIFDCEYCYLKGMYPSGNLVVFVNLE
EEEEECCCCCEECCCHHHHHCCCCCEEEHHHHHHHHHCCCCCEEEECCCCCCEEEEEEHH
DIFAELEALLAQKSIYLCVSYDADLVAIESLTGFVREWIAFTKKHENLTIEIRTKSGRCD
HHHHHHHHHHHCCCEEEEEECCCCEEHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCC
LWSNYEACDRVIIAYTVSPQRIAAEYEHFASAPMERIQAAKCAMDGGFPVRLCFDPMIYC
CCCCHHHHCEEEEEEECCHHHHHHHHHHHHHCHHHHHHHHHHHCCCCCEEEEEECCCEEE
KDWRGEYSRLVDDVFSQIDGFTLWDVSIGSFRISQDYLKKMRKDMPRSAVVNFPYDNVNG
ECCCCHHHHHHHHHHHHHCCEEEEEEECCCEEECHHHHHHHHHCCCCHHEEECCCCCCCC
YYQYPENIRSDMEEFMVQAVSEYVDKDRIFMWK
EEECCHHHHHHHHHHHHHHHHHHHCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11466286 [H]