Definition Eubacterium rectale ATCC 33656, complete genome.
Accession NC_012781
Length 3,449,685

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The map label for this gene is murB

Identifier: 238923104

GI number: 238923104

Start: 639492

End: 640436

Strand: Direct

Name: murB

Synonym: EUBREC_0696

Alternate gene names: 238923104

Gene position: 639492-640436 (Clockwise)

Preceding gene: 238923103

Following gene: 238923105

Centisome position: 18.54

GC content: 50.58

Gene sequence:

>945_bases
ATGAATCAGACATTTGTAAAAAGTGTAACAGAACAATTGCCGCAGCTTGGTCTCCTGCAGGATGAGCCGATGAAAAAACA
CACGACCTTCCGCATAGGAGGCCCGGCAGATTACTATGCAGAGCCTGATATGAGCCGGATTTCAAAGCTTATAGAGATGG
CTAAGGCATGTGATATGCCGGTTACAGTGATAGGAAACGGAAGCAATCTGCTGGTAGGAGATAAGGGCATTAGAGGCCTT
GTAATTGGTATTGGAAAAGGCCTGTCCGAAATCGAGGTGACAGAAGCTGTGGCGCAGGATTTCACAGCACAAGACAACTG
TCATATCATCACAGCGGGCGCCGGGGCTATCCTTGCGGCAGTGGCGGCAAAGGCAGCGGAGGCATCGCTTTCAGGTCTTG
AGTTTGCATCGGGCATCCCGGGAAGTGTTGGCGGAGCGGTAGTCATGAACGCAGGAGCCTACGGCGGAGAGATAAAGGAT
GTCCTAATCGATGCCACAGTGCTTACAGCAGATGGCGAGCTAAAGACTGTCACAAGGGATGAGCTTGACTTATCCTACAG
GCACAGTATAGTCCCGGAAAAGGGCTATATAGTGCTGTCAGCCAGATTCAGGCTCACACCAAAGCCAAAGGATGAGATAA
AGTCATATATGGCAGAGCTTCGCGCAAAGAGAGTGGAGAAGCAGCCGCTTGAGTACCCAAGTGCAGGCAGCACCTTTAAG
AGACCGGAGGGCTATTTTGCAGGGAAGCTTATCATGGATGCAGGTCTTCGAGGCTACAGCGTGGGAGATGCACAGGTGTC
GGAGAAGCACTGCGGCTTTGTAGTCAATAAGGGCGAAGCCGCTGCAGCGGATGTCCTGACACTCATAAAAGACGTGCAGG
AAACAGTTTTAAAGCAGTTTGGAGTAAAGCTCGAACCGGAAGTTAAGATGATTGGAGAATTCTAG

Upstream 100 bases:

>100_bases
ATGCATGCGCCATGGGAAATCATCAGCAAGGTCGATTTATATGAGGCATTCAGAGGCTACATTGCATTTTTGAAGGAAGC
ATAGGCTAAAGGAATATAAG

Downstream 100 bases:

>100_bases
TGAAATTTGTAATAGTGACGGGCATGTCAGGTGCCGGAAAAAGCACAGCAATGAAAATGATGGAGGATATGGGATATTTC
TGTATAGACAATCTGCCTAT

Product: UDP-N-acetylenolpyruvoylglucosamine reductase

Products: NA

Alternate protein names: UDP-N-acetylmuramate dehydrogenase

Number of amino acids: Translated: 314; Mature: 314

Protein sequence:

>314_residues
MNQTFVKSVTEQLPQLGLLQDEPMKKHTTFRIGGPADYYAEPDMSRISKLIEMAKACDMPVTVIGNGSNLLVGDKGIRGL
VIGIGKGLSEIEVTEAVAQDFTAQDNCHIITAGAGAILAAVAAKAAEASLSGLEFASGIPGSVGGAVVMNAGAYGGEIKD
VLIDATVLTADGELKTVTRDELDLSYRHSIVPEKGYIVLSARFRLTPKPKDEIKSYMAELRAKRVEKQPLEYPSAGSTFK
RPEGYFAGKLIMDAGLRGYSVGDAQVSEKHCGFVVNKGEAAAADVLTLIKDVQETVLKQFGVKLEPEVKMIGEF

Sequences:

>Translated_314_residues
MNQTFVKSVTEQLPQLGLLQDEPMKKHTTFRIGGPADYYAEPDMSRISKLIEMAKACDMPVTVIGNGSNLLVGDKGIRGL
VIGIGKGLSEIEVTEAVAQDFTAQDNCHIITAGAGAILAAVAAKAAEASLSGLEFASGIPGSVGGAVVMNAGAYGGEIKD
VLIDATVLTADGELKTVTRDELDLSYRHSIVPEKGYIVLSARFRLTPKPKDEIKSYMAELRAKRVEKQPLEYPSAGSTFK
RPEGYFAGKLIMDAGLRGYSVGDAQVSEKHCGFVVNKGEAAAADVLTLIKDVQETVLKQFGVKLEPEVKMIGEF
>Mature_314_residues
MNQTFVKSVTEQLPQLGLLQDEPMKKHTTFRIGGPADYYAEPDMSRISKLIEMAKACDMPVTVIGNGSNLLVGDKGIRGL
VIGIGKGLSEIEVTEAVAQDFTAQDNCHIITAGAGAILAAVAAKAAEASLSGLEFASGIPGSVGGAVVMNAGAYGGEIKD
VLIDATVLTADGELKTVTRDELDLSYRHSIVPEKGYIVLSARFRLTPKPKDEIKSYMAELRAKRVEKQPLEYPSAGSTFK
RPEGYFAGKLIMDAGLRGYSVGDAQVSEKHCGFVVNKGEAAAADVLTLIKDVQETVLKQFGVKLEPEVKMIGEF

Specific function: Cell wall formation

COG id: COG0812

COG function: function code M; UDP-N-acetylmuramate dehydrogenase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding PCMH-type domain

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MURB_EUBR3 (C4ZEG7)

Other databases:

- EMBL:   CP001107
- RefSeq:   YP_002936617.1
- GeneID:   7965979
- GenomeReviews:   CP001107_GR
- KEGG:   ere:EUBREC_0696
- GO:   GO:0005737
- HAMAP:   MF_00037
- InterPro:   IPR016169
- InterPro:   IPR016166
- InterPro:   IPR016167
- InterPro:   IPR003170
- InterPro:   IPR011601
- InterPro:   IPR006094
- Gene3D:   G3DSA:3.30.465.10
- Gene3D:   G3DSA:3.30.43.10
- Gene3D:   G3DSA:3.90.78.10
- PANTHER:   PTHR21071
- TIGRFAMs:   TIGR00179

Pfam domain/function: PF01565 FAD_binding_4; PF02873 MurB_C; SSF56176 FAD-binding_2; SSF56194 MurB_C

EC number: =1.1.1.158

Molecular weight: Translated: 33468; Mature: 33468

Theoretical pI: Translated: 5.27; Mature: 5.27

Prosite motif: PS51387 FAD_PCMH

Important sites: ACT_SITE 187-187 ACT_SITE 237-237 ACT_SITE 307-307

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure: 

>Translated Secondary Structure
MNQTFVKSVTEQLPQLGLLQDEPMKKHTTFRIGGPADYYAEPDMSRISKLIEMAKACDMP
CCHHHHHHHHHHHHHCCCCCCCCCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHCCCC
VTVIGNGSNLLVGDKGIRGLVIGIGKGLSEIEVTEAVAQDFTAQDNCHIITAGAGAILAA
EEEEECCCEEEEECCCCCEEEEECCCCCCHHHHHHHHHHHCCCCCCCEEEEECHHHHHHH
VAAKAAEASLSGLEFASGIPGSVGGAVVMNAGAYGGEIKDVLIDATVLTADGELKTVTRD
HHHHHHHHHHCCCHHHCCCCCCCCCEEEEECCCCCCCHHHEEEEEEEEECCCCEEEEEHH
ELDLSYRHSIVPEKGYIVLSARFRLTPKPKDEIKSYMAELRAKRVEKQPLEYPSAGSTFK
HHCHHHHHCCCCCCCEEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
RPEGYFAGKLIMDAGLRGYSVGDAQVSEKHCGFVVNKGEAAAADVLTLIKDVQETVLKQF
CCCCCCHHHHHHHCCCCCCCCCCCHHCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHC
GVKLEPEVKMIGEF
CCCCCCCHHCCCCC
>Mature Secondary Structure
MNQTFVKSVTEQLPQLGLLQDEPMKKHTTFRIGGPADYYAEPDMSRISKLIEMAKACDMP
CCHHHHHHHHHHHHHCCCCCCCCCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHCCCC
VTVIGNGSNLLVGDKGIRGLVIGIGKGLSEIEVTEAVAQDFTAQDNCHIITAGAGAILAA
EEEEECCCEEEEECCCCCEEEEECCCCCCHHHHHHHHHHHCCCCCCCEEEEECHHHHHHH
VAAKAAEASLSGLEFASGIPGSVGGAVVMNAGAYGGEIKDVLIDATVLTADGELKTVTRD
HHHHHHHHHHCCCHHHCCCCCCCCCEEEEECCCCCCCHHHEEEEEEEEECCCCEEEEEHH
ELDLSYRHSIVPEKGYIVLSARFRLTPKPKDEIKSYMAELRAKRVEKQPLEYPSAGSTFK
HHCHHHHHCCCCCCCEEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
RPEGYFAGKLIMDAGLRGYSVGDAQVSEKHCGFVVNKGEAAAADVLTLIKDVQETVLKQF
CCCCCCHHHHHHHCCCCCCCCCCCHHCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHC
GVKLEPEVKMIGEF
CCCCCCCHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA