Click here to switch to the map view.

The map label for this gene is pflB [H]

Identifier: 238922881

GI number: 238922881

Start: 427422

End: 429674

Strand: Direct

Name: pflB [H]

Synonym: EUBREC_0469

Alternate gene names: 238922881

Gene position: 427422-429674 (Clockwise)

Preceding gene: 238922879

Following gene: 238922883

Centisome position: 12.39

GC content: 45.18

Gene sequence:

>2253_bases
ATGATAGAAATGAAAGAGTGGGATGGATTTGAAGGACGTCTTTGGAAAGAGGAAATCAACGTCCGTCAGTTTATTCAGGA
CAACTACACTCCATACGACGGAGACGAGAGCTTTTTAGCTGATCCGACAGATGCTACAAACAAGCTGTGGGATGCACTCC
AGAAATTACAGAAAGAGGAGCGTGCCAAGGGCGGAGTTCTTGACATGGAGACAGAGGTTGTAACAGGAATCACAGCTTAC
GGCCCTGGATATATAGATGAGTCATTAAAGGACCTCGAGCAGATTGTAGGTCTTCAGACAGATAAGCCGTTAAAGAGAGC
ATTCATGCCTTACGGTGGAATCAAGATGGCGGTGCAGGCTGCTGAGACATATGGTTACAATGTAAGCGACAAGCTCAAGG
AGATTTTCACAAAATATCATAAGACACACAACACAGCAGTATTCGATGCATACACACCTGAGATGATGAAGGTCCGTCAT
GCAAAGATCCTTACAGGTCTTCCTGACACATACGGCCGTGGACGTATCGTGGGCGATTACCGTCGAGTAGCGTTATACGG
ACTTGATTATCTCATCGAGGAGAAAAAGAAGGATAAGGCAAACTGTGGCTGTGGACAGATGACAGATGATGTTATCCGTT
TAAGAGAGGAAATCGCAGAGCAGATTAAATGTCTTGAGGATATGAAGAAGCTTGCAGAGATTTACGGATATGATATCTCA
AGACCTGCAACAAACGCAAAAGAGGCAGTTCAGTGGTTATACTTCGGATACCTTGCAGCAATCAAGACACAGAATGGTGC
AGCAATGTCAGTAGGACGTGTATCTACATTCCTTGACATTTACATCAAGAGAGATATGGACAAGGGCATCCTCACAGAGC
AGGAAGCACAGGAGCTCATTGACCATTTTACAATGAAGCTTCGTATGGTTAAATTTGCACGTATCCCTTCATACAACCAG
TTATTCTCAGGTGATCCTGTATGGGCAACACTTGACGTGGCAGGAACAGGTGTGGACGGACGTTCAATGGTTACAAAGAC
AGACTTCCGTTTCCTCCATACATTAGAGAACATGGGACCTGCACCGGAGCCAAACCTCACAGTATTCTACTCATCAAAGC
TTCCTCAGACCTTCAAGGATTATGCAGCCCGCATCTCAATTGAGACAAGCTCAATCCAGTATGAGAACGATGATGCAATG
AAGCCTGTATGGGGCGATGATTATGCAATCTGCTGCTGTGTATCTGCAACACAGACAGGTAAAGAAATGCAGTTCTTCGG
AGCACGTGCGAACCTTGCAAAATGTCTCCTCTACGCTATCAACGGTGGTGTTGATGAGAAATCAGGTGAGCAGGTAGGAC
CAAACTACGCACCTATCACAGCAGAGTACCTTGACTATGACGAGGTAATGGCGAAATACGACAAGATGATGGACTGGTTA
GTAGACATTTATGTAAATACATTAAACCTTATCCAGTACATGCATGACAAATACTACTATGAGGCAGCAGAGCTTGCACT
CATGGATACAGACTTAAAGAGAACATTCGCAACAGGAATCGCAGGATTCTCACACGTAATCGATTCTCTTTCAGCAATCA
AATATGCAAAGGTTAAAGTAGTACGTGACGAGAGCGGACTGGCAAAGGATTTCGAAATTGAGGGAGAGTTCCCTAAATAC
GGAAACGATGATGACCGCGCGGACGAAATCGGTGTATGGCTTCTTAAGACATTCATGGACAAGTTAAAGAAGCATCACAC
ATACCGTGATTCAGAGCCTACAACATCAATCCTTACAATTACATCAAACGTAGTATACGGAAAAGCTACAGGAGCAATGC
CTGACGGACGTAAAGCCGGAGAGCCACTCTCACCTGGAGCAAACCCATCATACGGCGCAGAGCAGAACGGACTTCTCGCA
TCCTTAAACTCACTCACAAAGCTTCCATATGAGTGGGCACTTGATGGAATCTCAAATACACAGACCATCAACCCGGGAGC
ACTCGGAAACAACGAGGGTGAGAGAATTGACAACCTTGTAAACGTAATGGACGGATACTTCGATCAGGGTGCACATCACC
TCAATGTAAACGTATTCGGTAAAGAGAAGCTCATCGATGCAATGGAGCATCCTGAGAAGGAGGAGTACGCAAACTTCACA
ATCCGAGTATCAGGATACGCAGTTAAGTTCATCGACCTCACAAGAGAGCAGCAGCTCGATGTAATCTCACGTACATGCCA
CGAGAGAATGTAA

Upstream 100 bases:

>100_bases
TGGATATTGACAAAAATACTGTAGTAAGTATATACTAACCACAGTTAAACAAAACTAATCAAACGAAGTAACATTCACAC
ATTTTAAGGAGGATATTACA

Downstream 100 bases:

>100_bases
TATGATCTAAGGTGTCACAGAGTGTACTACGATTCTGCTTGCAGAAAGAGTAGTATGCTCTTGTGACCCGAACAAACATG
AGCAAGAAGCGATTTTCGTA

Product: hypothetical protein

Products: NA

Alternate protein names: Pyruvate formate-lyase [H]

Number of amino acids: Translated: 750; Mature: 750

Protein sequence:

>750_residues
MIEMKEWDGFEGRLWKEEINVRQFIQDNYTPYDGDESFLADPTDATNKLWDALQKLQKEERAKGGVLDMETEVVTGITAY
GPGYIDESLKDLEQIVGLQTDKPLKRAFMPYGGIKMAVQAAETYGYNVSDKLKEIFTKYHKTHNTAVFDAYTPEMMKVRH
AKILTGLPDTYGRGRIVGDYRRVALYGLDYLIEEKKKDKANCGCGQMTDDVIRLREEIAEQIKCLEDMKKLAEIYGYDIS
RPATNAKEAVQWLYFGYLAAIKTQNGAAMSVGRVSTFLDIYIKRDMDKGILTEQEAQELIDHFTMKLRMVKFARIPSYNQ
LFSGDPVWATLDVAGTGVDGRSMVTKTDFRFLHTLENMGPAPEPNLTVFYSSKLPQTFKDYAARISIETSSIQYENDDAM
KPVWGDDYAICCCVSATQTGKEMQFFGARANLAKCLLYAINGGVDEKSGEQVGPNYAPITAEYLDYDEVMAKYDKMMDWL
VDIYVNTLNLIQYMHDKYYYEAAELALMDTDLKRTFATGIAGFSHVIDSLSAIKYAKVKVVRDESGLAKDFEIEGEFPKY
GNDDDRADEIGVWLLKTFMDKLKKHHTYRDSEPTTSILTITSNVVYGKATGAMPDGRKAGEPLSPGANPSYGAEQNGLLA
SLNSLTKLPYEWALDGISNTQTINPGALGNNEGERIDNLVNVMDGYFDQGAHHLNVNVFGKEKLIDAMEHPEKEEYANFT
IRVSGYAVKFIDLTREQQLDVISRTCHERM

Sequences:

>Translated_750_residues
MIEMKEWDGFEGRLWKEEINVRQFIQDNYTPYDGDESFLADPTDATNKLWDALQKLQKEERAKGGVLDMETEVVTGITAY
GPGYIDESLKDLEQIVGLQTDKPLKRAFMPYGGIKMAVQAAETYGYNVSDKLKEIFTKYHKTHNTAVFDAYTPEMMKVRH
AKILTGLPDTYGRGRIVGDYRRVALYGLDYLIEEKKKDKANCGCGQMTDDVIRLREEIAEQIKCLEDMKKLAEIYGYDIS
RPATNAKEAVQWLYFGYLAAIKTQNGAAMSVGRVSTFLDIYIKRDMDKGILTEQEAQELIDHFTMKLRMVKFARIPSYNQ
LFSGDPVWATLDVAGTGVDGRSMVTKTDFRFLHTLENMGPAPEPNLTVFYSSKLPQTFKDYAARISIETSSIQYENDDAM
KPVWGDDYAICCCVSATQTGKEMQFFGARANLAKCLLYAINGGVDEKSGEQVGPNYAPITAEYLDYDEVMAKYDKMMDWL
VDIYVNTLNLIQYMHDKYYYEAAELALMDTDLKRTFATGIAGFSHVIDSLSAIKYAKVKVVRDESGLAKDFEIEGEFPKY
GNDDDRADEIGVWLLKTFMDKLKKHHTYRDSEPTTSILTITSNVVYGKATGAMPDGRKAGEPLSPGANPSYGAEQNGLLA
SLNSLTKLPYEWALDGISNTQTINPGALGNNEGERIDNLVNVMDGYFDQGAHHLNVNVFGKEKLIDAMEHPEKEEYANFT
IRVSGYAVKFIDLTREQQLDVISRTCHERM
>Mature_750_residues
MIEMKEWDGFEGRLWKEEINVRQFIQDNYTPYDGDESFLADPTDATNKLWDALQKLQKEERAKGGVLDMETEVVTGITAY
GPGYIDESLKDLEQIVGLQTDKPLKRAFMPYGGIKMAVQAAETYGYNVSDKLKEIFTKYHKTHNTAVFDAYTPEMMKVRH
AKILTGLPDTYGRGRIVGDYRRVALYGLDYLIEEKKKDKANCGCGQMTDDVIRLREEIAEQIKCLEDMKKLAEIYGYDIS
RPATNAKEAVQWLYFGYLAAIKTQNGAAMSVGRVSTFLDIYIKRDMDKGILTEQEAQELIDHFTMKLRMVKFARIPSYNQ
LFSGDPVWATLDVAGTGVDGRSMVTKTDFRFLHTLENMGPAPEPNLTVFYSSKLPQTFKDYAARISIETSSIQYENDDAM
KPVWGDDYAICCCVSATQTGKEMQFFGARANLAKCLLYAINGGVDEKSGEQVGPNYAPITAEYLDYDEVMAKYDKMMDWL
VDIYVNTLNLIQYMHDKYYYEAAELALMDTDLKRTFATGIAGFSHVIDSLSAIKYAKVKVVRDESGLAKDFEIEGEFPKY
GNDDDRADEIGVWLLKTFMDKLKKHHTYRDSEPTTSILTITSNVVYGKATGAMPDGRKAGEPLSPGANPSYGAEQNGLLA
SLNSLTKLPYEWALDGISNTQTINPGALGNNEGERIDNLVNVMDGYFDQGAHHLNVNVFGKEKLIDAMEHPEKEEYANFT
IRVSGYAVKFIDLTREQQLDVISRTCHERM

Specific function: Glucose metabolism (nonoxidative conversion). [C]

COG id: COG1882

COG function: function code C; Pyruvate-formate lyase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 pyruvate formate lyase domain [H]

Homologues:

Organism=Escherichia coli, GI1787131, Length=754, Percent_Identity=58.8859416445623, Blast_Score=915, Evalue=0.0,
Organism=Escherichia coli, GI48994926, Length=749, Percent_Identity=58.3444592790387, Blast_Score=894, Evalue=0.0,
Organism=Escherichia coli, GI1787044, Length=582, Percent_Identity=27.319587628866, Blast_Score=184, Evalue=2e-47,
Organism=Escherichia coli, GI1790388, Length=740, Percent_Identity=23.3783783783784, Blast_Score=143, Evalue=5e-35,
Organism=Escherichia coli, GI1788933, Length=62, Percent_Identity=59.6774193548387, Blast_Score=74, Evalue=4e-14,

Paralogues:

None

Copy number: 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005949
- InterPro:   IPR001150
- InterPro:   IPR019777
- InterPro:   IPR004184 [H]

Pfam domain/function: PF01228 Gly_radical; PF02901 PFL [H]

EC number: =2.3.1.54 [H]

Molecular weight: Translated: 84646; Mature: 84646

Theoretical pI: Translated: 4.79; Mature: 4.79

Prosite motif: PS00850 GLY_RADICAL_1 ; PS51149 GLY_RADICAL_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIEMKEWDGFEGRLWKEEINVRQFIQDNYTPYDGDESFLADPTDATNKLWDALQKLQKEE
CCCCCCCCCCCCEEEHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
RAKGGVLDMETEVVTGITAYGPGYIDESLKDLEQIVGLQTDKPLKRAFMPYGGIKMAVQA
HHCCCCEEECHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCHHHHEEH
AETYGYNVSDKLKEIFTKYHKTHNTAVFDAYTPEMMKVRHAKILTGLPDTYGRGRIVGDY
HHHCCCCHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHCCCCCCCCCCCEEHHH
RRVALYGLDYLIEEKKKDKANCGCGQMTDDVIRLREEIAEQIKCLEDMKKLAEIYGYDIS
HHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
RPATNAKEAVQWLYFGYLAAIKTQNGAAMSVGRVSTFLDIYIKRDMDKGILTEQEAQELI
CCCCCHHHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHCCCCCCCCCHHHHHHHH
DHFTMKLRMVKFARIPSYNQLFSGDPVWATLDVAGTGVDGRSMVTKTDFRFLHTLENMGP
HHHHHHHHHHHHHCCCCCHHHCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCC
APEPNLTVFYSSKLPQTFKDYAARISIETSSIQYENDDAMKPVWGDDYAICCCVSATQTG
CCCCCEEEEECCCCCHHHHHHHHEEEEEECEEEECCCCCCCCCCCCCCEEEEEEECCCCC
KEMQFFGARANLAKCLLYAINGGVDEKSGEQVGPNYAPITAEYLDYDEVMAKYDKMMDWL
CHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHCCHHHHHHHHHHHHHHH
VDIYVNTLNLIQYMHDKYYYEAAELALMDTDLKRTFATGIAGFSHVIDSLSAIKYAKVKV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEE
VRDESGLAKDFEIEGEFPKYGNDDDRADEIGVWLLKTFMDKLKKHHTYRDSEPTTSILTI
EECCCCCCCCEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEE
TSNVVYGKATGAMPDGRKAGEPLSPGANPSYGAEQNGLLASLNSLTKLPYEWALDGISNT
ECCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCHHHHHCCCCCC
QTINPGALGNNEGERIDNLVNVMDGYFDQGAHHLNVNVFGKEKLIDAMEHPEKEEYANFT
CCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEEEECHHHHHHHHHCCCCHHHCCEE
IRVSGYAVKFIDLTREQQLDVISRTCHERM
EEEECEEEEEEECCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MIEMKEWDGFEGRLWKEEINVRQFIQDNYTPYDGDESFLADPTDATNKLWDALQKLQKEE
CCCCCCCCCCCCEEEHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
RAKGGVLDMETEVVTGITAYGPGYIDESLKDLEQIVGLQTDKPLKRAFMPYGGIKMAVQA
HHCCCCEEECHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCHHHHEEH
AETYGYNVSDKLKEIFTKYHKTHNTAVFDAYTPEMMKVRHAKILTGLPDTYGRGRIVGDY
HHHCCCCHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHCCCCCCCCCCCEEHHH
RRVALYGLDYLIEEKKKDKANCGCGQMTDDVIRLREEIAEQIKCLEDMKKLAEIYGYDIS
HHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
RPATNAKEAVQWLYFGYLAAIKTQNGAAMSVGRVSTFLDIYIKRDMDKGILTEQEAQELI
CCCCCHHHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHCCCCCCCCCHHHHHHHH
DHFTMKLRMVKFARIPSYNQLFSGDPVWATLDVAGTGVDGRSMVTKTDFRFLHTLENMGP
HHHHHHHHHHHHHCCCCCHHHCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCC
APEPNLTVFYSSKLPQTFKDYAARISIETSSIQYENDDAMKPVWGDDYAICCCVSATQTG
CCCCCEEEEECCCCCHHHHHHHHEEEEEECEEEECCCCCCCCCCCCCCEEEEEEECCCCC
KEMQFFGARANLAKCLLYAINGGVDEKSGEQVGPNYAPITAEYLDYDEVMAKYDKMMDWL
CHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHCCHHHHHHHHHHHHHHH
VDIYVNTLNLIQYMHDKYYYEAAELALMDTDLKRTFATGIAGFSHVIDSLSAIKYAKVKV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEE
VRDESGLAKDFEIEGEFPKYGNDDDRADEIGVWLLKTFMDKLKKHHTYRDSEPTTSILTI
EECCCCCCCCEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEE
TSNVVYGKATGAMPDGRKAGEPLSPGANPSYGAEQNGLLASLNSLTKLPYEWALDGISNT
ECCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCHHHHHCCCCCC
QTINPGALGNNEGERIDNLVNVMDGYFDQGAHHLNVNVFGKEKLIDAMEHPEKEEYANFT
CCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEEEECHHHHHHHHHCCCCHHHCCEE
IRVSGYAVKFIDLTREQQLDVISRTCHERM
EEEECEEEEEEECCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA