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The map label for this gene is act [H]

Identifier: 238922883

GI number: 238922883

Start: 430154

End: 430903

Strand: Direct

Name: act [H]

Synonym: EUBREC_0471

Alternate gene names: 238922883

Gene position: 430154-430903 (Clockwise)

Preceding gene: 238922881

Following gene: 238922884

Centisome position: 12.47

GC content: 44.4

Gene sequence:

>750_bases
ATGGGAGAAACACTAGGAAGAGTACACTCGGTAGAAAGCTTCGGTTCAGCCGACGGCCCGGGAGTGAGATATATAGTATT
TTTGAAGGGCTGCAATATGCGCTGTAAATACTGCCACAATCCGGATACATGGGCCAAATGCGGCGAAAATGACGGTGCAA
AGCTGATGACACCGCAGGAGGTGCTAAAGACCGCCATGCGCTATAAAGCGTACTGGAAGCAAACAGGCGGCATTACAGTA
AGCGGAGGTGAAGCACTTTTGCAGATAGACTTTGTCACAGAGCTATTTAAGCTTGCAAAGGAAAAAGGTGTAAACACATG
CCTTGACACATCAGGAAATCCGTTTACCGTGGAAGAACCGTTCTTCGGCAAATTTAATGAGCTGATGAAATATACAGATC
TTTTCATGCTGGACATCAAGCATATAGATGACGAGGAGCACAAAAAGCTTACGGGACAGACAAATAAGAATATCCTTGAT
ATGGCACAATATCTCTCAAAAAACGGTAAAAAAATGTGGATACGTCATGTGCTTGTGCCTGGAATCACCACGGATGAGAG
ATATTTAAATCAGCTTAGAGAGTTTATCGACACACTTAAAACCGTGGACAGGGTTGAGGTGCTGCCGTACCACACACTTG
GGGTGTTCAAGTGGAAGGAGCTTGGTATACCATACCAGCTTGAGGGGGTGGAACCTCCGACAAAGGAACAGATAGAATGT
GCAAAAAGAATATTAAATTGTCAGAATTAG

Upstream 100 bases:

>100_bases
GAAGAACCTGAGCATTGCAAAGTCCCGGTCGGCGGGTTGCCGAAAATTCCGTACCATAAAAATAAAAGTAGCAGCAACAA
CAACCTAAAGGAGGAAAAAC

Downstream 100 bases:

>100_bases
TTTTTAAATATCATTGCTAACAACTCATTAAGTTGATAAAATATAATAAGTAACTATTATTTTACACCGATGCTGCCTTC
GTTTGTATGTGAGGTGACAC

Product: pyruvate formate-lyase activating enzyme

Products: NA

Alternate protein names: PFL-activating enzyme; Formate-C-acetyltransferase-activating enzyme [H]

Number of amino acids: Translated: 249; Mature: 248

Protein sequence:

>249_residues
MGETLGRVHSVESFGSADGPGVRYIVFLKGCNMRCKYCHNPDTWAKCGENDGAKLMTPQEVLKTAMRYKAYWKQTGGITV
SGGEALLQIDFVTELFKLAKEKGVNTCLDTSGNPFTVEEPFFGKFNELMKYTDLFMLDIKHIDDEEHKKLTGQTNKNILD
MAQYLSKNGKKMWIRHVLVPGITTDERYLNQLREFIDTLKTVDRVEVLPYHTLGVFKWKELGIPYQLEGVEPPTKEQIEC
AKRILNCQN

Sequences:

>Translated_249_residues
MGETLGRVHSVESFGSADGPGVRYIVFLKGCNMRCKYCHNPDTWAKCGENDGAKLMTPQEVLKTAMRYKAYWKQTGGITV
SGGEALLQIDFVTELFKLAKEKGVNTCLDTSGNPFTVEEPFFGKFNELMKYTDLFMLDIKHIDDEEHKKLTGQTNKNILD
MAQYLSKNGKKMWIRHVLVPGITTDERYLNQLREFIDTLKTVDRVEVLPYHTLGVFKWKELGIPYQLEGVEPPTKEQIEC
AKRILNCQN
>Mature_248_residues
GETLGRVHSVESFGSADGPGVRYIVFLKGCNMRCKYCHNPDTWAKCGENDGAKLMTPQEVLKTAMRYKAYWKQTGGITVS
GGEALLQIDFVTELFKLAKEKGVNTCLDTSGNPFTVEEPFFGKFNELMKYTDLFMLDIKHIDDEEHKKLTGQTNKNILDM
AQYLSKNGKKMWIRHVLVPGITTDERYLNQLREFIDTLKTVDRVEVLPYHTLGVFKWKELGIPYQLEGVEPPTKEQIECA
KRILNCQN

Specific function: Activation of pyruvate formate-lyase under anaerobic conditions by generation of an organic free radical, using S- adenosylmethionine and reduced flavodoxin as cosubstrates to produce 5'-deoxy-adenosine [H]

COG id: COG1180

COG function: function code O; Pyruvate-formate lyase-activating enzyme

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the organic radical-activating enzymes family [H]

Homologues:

Organism=Escherichia coli, GI1787130, Length=243, Percent_Identity=43.6213991769547, Blast_Score=219, Evalue=1e-58,
Organism=Escherichia coli, GI1790839, Length=275, Percent_Identity=28, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI1790389, Length=263, Percent_Identity=23.1939163498099, Blast_Score=92, Evalue=2e-20,
Organism=Escherichia coli, GI226510931, Length=181, Percent_Identity=31.4917127071823, Blast_Score=82, Evalue=4e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR012838
- InterPro:   IPR001989
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =1.97.1.4 [H]

Molecular weight: Translated: 28440; Mature: 28309

Theoretical pI: Translated: 7.43; Mature: 7.43

Prosite motif: PS01087 RADICAL_ACTIVATING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGETLGRVHSVESFGSADGPGVRYIVFLKGCNMRCKYCHNPDTWAKCGENDGAKLMTPQE
CCCHHHHHHHHHHCCCCCCCCEEEEEEEECCCCEEEECCCCCHHHHCCCCCCCCCCCHHH
VLKTAMRYKAYWKQTGGITVSGGEALLQIDFVTELFKLAKEKGVNTCLDTSGNPFTVEEP
HHHHHHHHHHHHHHCCCEEEECCCEEEEHHHHHHHHHHHHHCCCCEECCCCCCCEEECCC
FFGKFNELMKYTDLFMLDIKHIDDEEHKKLTGQTNKNILDMAQYLSKNGKKMWIRHVLVP
CHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCHHHHHHHHHHCCCHHEEHHHHHCC
GITTDERYLNQLREFIDTLKTVDRVEVLPYHTLGVFKWKELGIPYQLEGVEPPTKEQIEC
CCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHCEEEHHHCCCCEEECCCCCCCHHHHHH
AKRILNCQN
HHHHHCCCC
>Mature Secondary Structure 
GETLGRVHSVESFGSADGPGVRYIVFLKGCNMRCKYCHNPDTWAKCGENDGAKLMTPQE
CCHHHHHHHHHHCCCCCCCCEEEEEEEECCCCEEEECCCCCHHHHCCCCCCCCCCCHHH
VLKTAMRYKAYWKQTGGITVSGGEALLQIDFVTELFKLAKEKGVNTCLDTSGNPFTVEEP
HHHHHHHHHHHHHHCCCEEEECCCEEEEHHHHHHHHHHHHHCCCCEECCCCCCCEEECCC
FFGKFNELMKYTDLFMLDIKHIDDEEHKKLTGQTNKNILDMAQYLSKNGKKMWIRHVLVP
CHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCHHHHHHHHHHCCCHHEEHHHHHCC
GITTDERYLNQLREFIDTLKTVDRVEVLPYHTLGVFKWKELGIPYQLEGVEPPTKEQIEC
CCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHCEEEHHHCCCCEEECCCCCCCHHHHHH
AKRILNCQN
HHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11029425; 10899886; 12397186 [H]