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The map label for this gene is aspS [H]

Identifier: 238922193

GI number: 238922193

Start: 390101

End: 391897

Strand: Reverse

Name: aspS [H]

Synonym: EUBELI_20428

Alternate gene names: 238922193

Gene position: 391897-390101 (Counterclockwise)

Preceding gene: 238922194

Following gene: 238922191

Centisome position: 62.53

GC content: 39.07

Gene sequence:

>1797_bases
ATGGCAGAGTCAATGGTTGGCTTAAAGAGATCGCACAGATGTACAGAGGTTTCATCGGCTGATATTGGTAAGAAGGTTAC
TGTAATGGGTTGGGTTGCCAAGAGAAGAAATCTTGGTTCACTTATTTTTGTTGATTTAAGAGACAGAAGTGGAATCCTTC
AGATTCTTTTTGATGAAAATATAATTGGTAAGGAAGGCTTCGACAAGGCATATACACTTCGTAATGAATTCGTAATCGCT
GTTGAGGGTGAAGTTATTAAGAGAAGTGGTGCTGTTAATGAGAATTTAAAGACAGGTGACATTGAGATTGCTGCAAAGAC
TTTAAGAATTCTTTCAGAGTCAGAGACACCTCCGTTCCCAATAGAGGAAAATATCCAGACTAAGGAAGATATCAGACTTA
AGTACAGATGTCTTGATCTTAGAAGACCTGATATCCAGTCTAATATTATTATGAGAAGCAAGGTTGCAACTCTTACAAGA
GCATTTCTTGCAAATGAAGGCTTTTTAGAGATTGAAACACCTATGCTTACCAAGTCTACACCAGAGGGAGCAAGAGATTA
TCTTGTACCAAGCCGTATTCATCCAGGTAAGTTTTATGCGCTTCCACAGTCACCACAGCTCTTTAAGCAGATGCTTATGT
GTTCTGGCTATGACAGATATATGCAGATTGCAAGATGCTTCAGAGATGAGGATTTAAGAGCAGACAGACAGCCTGAATTT
ACACAGATAGATATGGAATTATCATTCGTTGATGTTGATGATGTAATCGATGTTAATGAAAGACTTCTTGCATACCTTTT
TAAGGAAGTTCTTGATGTTGATGTAAAGCTTCCAATACAGAGAATGACATGGCAGGAAGCTATGGACAGATTTGGTTCTG
ATAAGCCTGATTTAAGATTTGGTATGGAATTACAGAATGTATCAGATATTGTTAAGGATTGTGGCTTTGGAGTATTTACG
GGAGCACTTGAGAATGGCGGTTCTGTAAGAGGAATCAACGCAAAGGGACAGGGAAGCATGCCTCGTAAGAAGATTGATGC
ACTTGTTGATTTTGCTAAGGGATATGGTGCAAAAGGTCTTGCTTATATTGCAATTCACGAAGATGGTTCATATAAGTCAT
CATTTGCAAAGTTTATGACAGAGGATCAGATGAATGCCCTTGTTAAGGCTATGGATGGTGAAGCAGGGGATCTTCTTTTA
TTCGCTGCTGACAGAAATAAGATTGTATGGAATGTACTAGGTGCTTTAAGAGTAGAACTTGCTAATCAAATGGGACTTCT
TGATAAGTCTGATTATAAGTTCTTATGGGTAACAGAGTTTCCACAGTTTGAATGGTCAGATGAAGAAGAAAGATATGTTG
CTATGCATCATCCATTCACAATGCCTATGGATGAGGATCTTGATATGCTTGAGACTAATCCTGGTGCAGTTCGTGCAAAG
GCTTATGATATTGTTCTTAACGGAACAGAAATCGGTGGTGGTTCAGTAAGAATCCATCAGGCTGATGTTCAGAGCAGAAT
GTTTAAGGCGCTTGGACTTACAGATGAAGTTGCTAATGAAAAGTTTGGATTCCTGCTTGACGCATTCAAGTATGGTGTTC
CTCCACATGCAGGACTTGCTTATGGCCTTGACAGACTTGTAATGCTTATGGCTAAGAGAGATTCAATCAGAGATGTTATT
GCCTTCCCTAAGGTAAAGGATGCAACTTGTCTCCTTACTAATGCACCTGATGTTGTAGATGCTAAGCAGCTTGATGAGCT
TTCAATTAAGATTGAAGAAGAGAAAACTGAAGAGTAA

Upstream 100 bases:

>100_bases
GATAATAAGAAATAGTAAGATAGATTTAGATAGTCTTAGTCTTGAAGATATTCTATCCTTCGCAGATTAGTATTTAATTA
CAGGAAAGAGGTAAAGTAAG

Downstream 100 bases:

>100_bases
GATATAATAAATGCCCTGCACTGATTTGAAAGTGCGGGGCATCATTTTATATGATATTTATAAAAATGTTATTTGATAGT
AATCTGTTCAACAGATGTAT

Product: aspartyl-tRNA synthetase

Products: NA

Alternate protein names: Aspartate--tRNA ligase; AspRS [H]

Number of amino acids: Translated: 598; Mature: 597

Protein sequence:

>598_residues
MAESMVGLKRSHRCTEVSSADIGKKVTVMGWVAKRRNLGSLIFVDLRDRSGILQILFDENIIGKEGFDKAYTLRNEFVIA
VEGEVIKRSGAVNENLKTGDIEIAAKTLRILSESETPPFPIEENIQTKEDIRLKYRCLDLRRPDIQSNIIMRSKVATLTR
AFLANEGFLEIETPMLTKSTPEGARDYLVPSRIHPGKFYALPQSPQLFKQMLMCSGYDRYMQIARCFRDEDLRADRQPEF
TQIDMELSFVDVDDVIDVNERLLAYLFKEVLDVDVKLPIQRMTWQEAMDRFGSDKPDLRFGMELQNVSDIVKDCGFGVFT
GALENGGSVRGINAKGQGSMPRKKIDALVDFAKGYGAKGLAYIAIHEDGSYKSSFAKFMTEDQMNALVKAMDGEAGDLLL
FAADRNKIVWNVLGALRVELANQMGLLDKSDYKFLWVTEFPQFEWSDEEERYVAMHHPFTMPMDEDLDMLETNPGAVRAK
AYDIVLNGTEIGGGSVRIHQADVQSRMFKALGLTDEVANEKFGFLLDAFKYGVPPHAGLAYGLDRLVMLMAKRDSIRDVI
AFPKVKDATCLLTNAPDVVDAKQLDELSIKIEEEKTEE

Sequences:

>Translated_598_residues
MAESMVGLKRSHRCTEVSSADIGKKVTVMGWVAKRRNLGSLIFVDLRDRSGILQILFDENIIGKEGFDKAYTLRNEFVIA
VEGEVIKRSGAVNENLKTGDIEIAAKTLRILSESETPPFPIEENIQTKEDIRLKYRCLDLRRPDIQSNIIMRSKVATLTR
AFLANEGFLEIETPMLTKSTPEGARDYLVPSRIHPGKFYALPQSPQLFKQMLMCSGYDRYMQIARCFRDEDLRADRQPEF
TQIDMELSFVDVDDVIDVNERLLAYLFKEVLDVDVKLPIQRMTWQEAMDRFGSDKPDLRFGMELQNVSDIVKDCGFGVFT
GALENGGSVRGINAKGQGSMPRKKIDALVDFAKGYGAKGLAYIAIHEDGSYKSSFAKFMTEDQMNALVKAMDGEAGDLLL
FAADRNKIVWNVLGALRVELANQMGLLDKSDYKFLWVTEFPQFEWSDEEERYVAMHHPFTMPMDEDLDMLETNPGAVRAK
AYDIVLNGTEIGGGSVRIHQADVQSRMFKALGLTDEVANEKFGFLLDAFKYGVPPHAGLAYGLDRLVMLMAKRDSIRDVI
AFPKVKDATCLLTNAPDVVDAKQLDELSIKIEEEKTEE
>Mature_597_residues
AESMVGLKRSHRCTEVSSADIGKKVTVMGWVAKRRNLGSLIFVDLRDRSGILQILFDENIIGKEGFDKAYTLRNEFVIAV
EGEVIKRSGAVNENLKTGDIEIAAKTLRILSESETPPFPIEENIQTKEDIRLKYRCLDLRRPDIQSNIIMRSKVATLTRA
FLANEGFLEIETPMLTKSTPEGARDYLVPSRIHPGKFYALPQSPQLFKQMLMCSGYDRYMQIARCFRDEDLRADRQPEFT
QIDMELSFVDVDDVIDVNERLLAYLFKEVLDVDVKLPIQRMTWQEAMDRFGSDKPDLRFGMELQNVSDIVKDCGFGVFTG
ALENGGSVRGINAKGQGSMPRKKIDALVDFAKGYGAKGLAYIAIHEDGSYKSSFAKFMTEDQMNALVKAMDGEAGDLLLF
AADRNKIVWNVLGALRVELANQMGLLDKSDYKFLWVTEFPQFEWSDEEERYVAMHHPFTMPMDEDLDMLETNPGAVRAKA
YDIVLNGTEIGGGSVRIHQADVQSRMFKALGLTDEVANEKFGFLLDAFKYGVPPHAGLAYGLDRLVMLMAKRDSIRDVIA
FPKVKDATCLLTNAPDVVDAKQLDELSIKIEEEKTEE

Specific function: Unknown

COG id: COG0173

COG function: function code J; Aspartyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI40789249, Length=594, Percent_Identity=39.8989898989899, Blast_Score=426, Evalue=1e-119,
Organism=Homo sapiens, GI194272210, Length=280, Percent_Identity=28.9285714285714, Blast_Score=94, Evalue=3e-19,
Organism=Homo sapiens, GI5031815, Length=280, Percent_Identity=28.9285714285714, Blast_Score=94, Evalue=3e-19,
Organism=Homo sapiens, GI45439306, Length=291, Percent_Identity=26.8041237113402, Blast_Score=86, Evalue=1e-16,
Organism=Homo sapiens, GI226491502, Length=253, Percent_Identity=23.7154150197628, Blast_Score=74, Evalue=5e-13,
Organism=Escherichia coli, GI1788173, Length=589, Percent_Identity=50.4244482173175, Blast_Score=576, Evalue=1e-165,
Organism=Escherichia coli, GI1790571, Length=289, Percent_Identity=27.3356401384083, Blast_Score=104, Evalue=1e-23,
Organism=Escherichia coli, GI1789256, Length=283, Percent_Identity=26.8551236749117, Blast_Score=102, Evalue=7e-23,
Organism=Caenorhabditis elegans, GI32566633, Length=600, Percent_Identity=33.6666666666667, Blast_Score=305, Evalue=3e-83,
Organism=Caenorhabditis elegans, GI71994340, Length=297, Percent_Identity=25.5892255892256, Blast_Score=90, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17535925, Length=297, Percent_Identity=25.5892255892256, Blast_Score=90, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI17535927, Length=297, Percent_Identity=25.5892255892256, Blast_Score=89, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI17551876, Length=279, Percent_Identity=25.089605734767, Blast_Score=80, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6325153, Length=631, Percent_Identity=32.4881141045959, Blast_Score=277, Evalue=4e-75,
Organism=Saccharomyces cerevisiae, GI6323011, Length=274, Percent_Identity=29.9270072992701, Blast_Score=91, Evalue=5e-19,
Organism=Saccharomyces cerevisiae, GI6320242, Length=256, Percent_Identity=25, Blast_Score=70, Evalue=7e-13,
Organism=Saccharomyces cerevisiae, GI6324256, Length=251, Percent_Identity=24.7011952191235, Blast_Score=69, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6321807, Length=266, Percent_Identity=24.0601503759398, Blast_Score=65, Evalue=3e-11,
Organism=Drosophila melanogaster, GI24584738, Length=604, Percent_Identity=37.7483443708609, Blast_Score=376, Evalue=1e-104,
Organism=Drosophila melanogaster, GI17136276, Length=298, Percent_Identity=28.1879194630872, Blast_Score=99, Evalue=6e-21,
Organism=Drosophila melanogaster, GI24640849, Length=252, Percent_Identity=26.984126984127, Blast_Score=82, Evalue=8e-16,
Organism=Drosophila melanogaster, GI24640851, Length=252, Percent_Identity=26.984126984127, Blast_Score=82, Evalue=1e-15,

Paralogues:

None

Copy number: 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR020564
- InterPro:   IPR004524
- InterPro:   IPR018153
- InterPro:   IPR002312
- InterPro:   IPR004115
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365 [H]

Pfam domain/function: PF02938 GAD; PF00152 tRNA-synt_2; PF01336 tRNA_anti [H]

EC number: =6.1.1.12 [H]

Molecular weight: Translated: 67383; Mature: 67252

Theoretical pI: Translated: 4.85; Mature: 4.85

Prosite motif: PS00037 MYB_1 ; PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAESMVGLKRSHRCTEVSSADIGKKVTVMGWVAKRRNLGSLIFVDLRDRSGILQILFDEN
CCCCHHHHHHCCCCCCCCCCCCCCEEEEEEHHHHHCCCCCEEEEEECCCCCEEEEEECCC
IIGKEGFDKAYTLRNEFVIAVEGEVIKRSGAVNENLKTGDIEIAAKTLRILSESETPPFP
CCCCCCCCHHEEECCCEEEEECCCEEECCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCC
IEENIQTKEDIRLKYRCLDLRRPDIQSNIIMRSKVATLTRAFLANEGFLEIETPMLTKST
CCCCCCCCHHHEEEEEEEECCCCCCHHCHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCC
PEGARDYLVPSRIHPGKFYALPQSPQLFKQMLMCSGYDRYMQIARCFRDEDLRADRQPEF
CCCCHHCCCCCCCCCCEEEECCCCHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCE
TQIDMELSFVDVDDVIDVNERLLAYLFKEVLDVDVKLPIQRMTWQEAMDRFGSDKPDLRF
EEEEEEEEEECHHHHHCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCEE
GMELQNVSDIVKDCGFGVFTGALENGGSVRGINAKGQGSMPRKKIDALVDFAKGYGAKGL
CCCHHHHHHHHHHCCCCEEEHHHCCCCCEEEECCCCCCCCCHHHHHHHHHHHHCCCCCCE
AYIAIHEDGSYKSSFAKFMTEDQMNALVKAMDGEAGDLLLFAADRNKIVWNVLGALRVEL
EEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCHHHHHHHHHHHH
ANQMGLLDKSDYKFLWVTEFPQFEWSDEEERYVAMHHPFTMPMDEDLDMLETNPGAVRAK
HHHHCCCCCCCCCEEEEEECCCCCCCCCCCCEEEEECCCCCCCCCCCHHHCCCCCEEEEE
AYDIVLNGTEIGGGSVRIHQADVQSRMFKALGLTDEVANEKFGFLLDAFKYGVPPHAGLA
EEEEEEECCEECCCEEEEEHHHHHHHHHHHHCCCHHHHCCHHHHHHHHHHCCCCCCCHHH
YGLDRLVMLMAKRDSIRDVIAFPKVKDATCLLTNAPDVVDAKQLDELSIKIEEEKTEE
HHHHHHHHHHHCCCHHHHHHCCCCCCCCEEEEECCCCCCCHHHHCCEEEEEECCCCCC
>Mature Secondary Structure 
AESMVGLKRSHRCTEVSSADIGKKVTVMGWVAKRRNLGSLIFVDLRDRSGILQILFDEN
CCCHHHHHHCCCCCCCCCCCCCCEEEEEEHHHHHCCCCCEEEEEECCCCCEEEEEECCC
IIGKEGFDKAYTLRNEFVIAVEGEVIKRSGAVNENLKTGDIEIAAKTLRILSESETPPFP
CCCCCCCCHHEEECCCEEEEECCCEEECCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCC
IEENIQTKEDIRLKYRCLDLRRPDIQSNIIMRSKVATLTRAFLANEGFLEIETPMLTKST
CCCCCCCCHHHEEEEEEEECCCCCCHHCHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCC
PEGARDYLVPSRIHPGKFYALPQSPQLFKQMLMCSGYDRYMQIARCFRDEDLRADRQPEF
CCCCHHCCCCCCCCCCEEEECCCCHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCE
TQIDMELSFVDVDDVIDVNERLLAYLFKEVLDVDVKLPIQRMTWQEAMDRFGSDKPDLRF
EEEEEEEEEECHHHHHCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCEE
GMELQNVSDIVKDCGFGVFTGALENGGSVRGINAKGQGSMPRKKIDALVDFAKGYGAKGL
CCCHHHHHHHHHHCCCCEEEHHHCCCCCEEEECCCCCCCCCHHHHHHHHHHHHCCCCCCE
AYIAIHEDGSYKSSFAKFMTEDQMNALVKAMDGEAGDLLLFAADRNKIVWNVLGALRVEL
EEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCHHHHHHHHHHHH
ANQMGLLDKSDYKFLWVTEFPQFEWSDEEERYVAMHHPFTMPMDEDLDMLETNPGAVRAK
HHHHCCCCCCCCCEEEEEECCCCCCCCCCCCEEEEECCCCCCCCCCCHHHCCCCCEEEEE
AYDIVLNGTEIGGGSVRIHQADVQSRMFKALGLTDEVANEKFGFLLDAFKYGVPPHAGLA
EEEEEEECCEECCCEEEEEHHHHHHHHHHHHCCCHHHHCCHHHHHHHHHHCCCCCCCHHH
YGLDRLVMLMAKRDSIRDVIAFPKVKDATCLLTNAPDVVDAKQLDELSIKIEEEKTEE
HHHHHHHHHHHCCCHHHHHHCCCCCCCCEEEEECCCCCCCHHHHCCEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA