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The map label for this gene is dctA

Identifier: 238921597

GI number: 238921597

Start: 3638106

End: 3639386

Strand: Reverse

Name: dctA

Synonym: NT01EI_3750

Alternate gene names: 238921597

Gene position: 3639386-3638106 (Counterclockwise)

Preceding gene: 238921598

Following gene: 238921596

Centisome position: 95.46

GC content: 55.97

Gene sequence:

>1281_bases
ATGAAAAAAAGTATCTTCACGAGCCTCTATCTACAGGTGTTGGTGGCTATCACTATTGGTATTTTGCTCGGCCATTTCTA
TCCCGATCTGGGTACCCAGATGAAGCCGTTGGGCGATGGGTTTGTCAAACTGATTAAGATGATCATCGCACCGGTGATCT
TTTGTACTGTGGTGACCGGTATTGCCGGTATGGAGAGCATGAAAGCGGTGGGGCGCACCGGTGCCATTGCGCTGCTCTAT
TTTGAGGTGGTCAGTACCATCGCGCTGATCATCGGCCTGGTGGTGGTTAACATACTGCAGCCGGGCGTAGGTATGAACGT
CGACCCGAACGCGCTGGATACCAAGGCGGTCGCGGTCTATGCCGATCAGGCGGCGCAGCAGGGCATTGTGGCTTTCCTGC
TCGATGTGATCCCCAGCAGCGTTATCGGTGCCTTTGCCAGCGGCAACATCCTGCAGGTGCTGCTGTTTGCGGTGATGTTT
GGTTTTGCGCTGCACCATTTGGGAGAAAAAGGGCAGTTGATCTTCAATGTGATCGACAGCTTTGCCAAAGTGATTTTTGG
CGTGATTAACATGATTATGAAGCTGGCCCCCATCGGTGCCTTCGGCGCCATGGCGTTCACCATCGGTAAGTATGGGGTGG
GAACGCTGGTACAGCTGGGGCAGTTGATCCTGTGCTTCTATATCACCTGCGCCCTGTTTGTGGTGCTGGTGCTGGGCAGC
ATCGCCAAGGCGACCGGGTTCAGTATCTTCCGCTTTATTGCCTACATTAAGGAGGAGCTGCTTATCGTACTGGGTACTTC
TTCCTCGGAGTCGGCACTGCCACGGATGCTGGACAAGATGGAGAAAGTGGGCTGCCAGAAGTCGGTGGTGGGGCTGGTGA
TCCCCACTGGCTACTCCTTTAATCTGGACGGAACCTCTATCTATCTGACCATGGCGGCGGTATTTATCGCCCAGGCGACC
AATGCCCAGATGGATATCTGGCATCAAGTGACGTTGCTGGTGGTCCTGCTGCTCTCCTCGAAGGGTGCCGCCGGCGTGAC
CGGCAGCGGCTTCATCGTCTTGGCTGCGACCCTGTCGGCGGTAGGGCATCTTCCGGTAGCCGGGTTGGCACTGATCCTGG
GCATCGATCGCTTCATGTCGGAAGCCCGGGCCCTGACCAACCTGATCGGCAACGGTGTGGCGACGATCGTTGTCGCCAAG
CGCTGTCGTCAACTGAATGAAAAACAGATGAATGCCGTACTCGGAGGCCGTGACAGCGCTCTGAGGGATCCCATCGCATA
G

Upstream 100 bases:

>100_bases
GTTTTCATATTGTTAACTTAATGTTACTTTTAAACGCATGCAATACCCGCCATTCGGGATGCAGAAGCTGCGCGTTCGTA
CCCGACCAGGAATAAAAATC

Downstream 100 bases:

>100_bases
CGCCTTTCCAGCCCGGCAGCAAGAGTGTGCCGTCGCGTTCTCCTCTTATTCCGCCATCGCCCCCGGCTTTTTGTTACCGG
GGGTGAATGTTCCCCCCCGG

Product: C4-dicarboxylate transporter DctA

Products: orotate [Cytoplasm]; fumarate [Cytoplasm]; malate [Cytoplasm]; Na (I) [Cytoplasm]; succinate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 426; Mature: 426

Protein sequence:

>426_residues
MKKSIFTSLYLQVLVAITIGILLGHFYPDLGTQMKPLGDGFVKLIKMIIAPVIFCTVVTGIAGMESMKAVGRTGAIALLY
FEVVSTIALIIGLVVVNILQPGVGMNVDPNALDTKAVAVYADQAAQQGIVAFLLDVIPSSVIGAFASGNILQVLLFAVMF
GFALHHLGEKGQLIFNVIDSFAKVIFGVINMIMKLAPIGAFGAMAFTIGKYGVGTLVQLGQLILCFYITCALFVVLVLGS
IAKATGFSIFRFIAYIKEELLIVLGTSSSESALPRMLDKMEKVGCQKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQAT
NAQMDIWHQVTLLVVLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLIGNGVATIVVAK
RCRQLNEKQMNAVLGGRDSALRDPIA

Sequences:

>Translated_426_residues
MKKSIFTSLYLQVLVAITIGILLGHFYPDLGTQMKPLGDGFVKLIKMIIAPVIFCTVVTGIAGMESMKAVGRTGAIALLY
FEVVSTIALIIGLVVVNILQPGVGMNVDPNALDTKAVAVYADQAAQQGIVAFLLDVIPSSVIGAFASGNILQVLLFAVMF
GFALHHLGEKGQLIFNVIDSFAKVIFGVINMIMKLAPIGAFGAMAFTIGKYGVGTLVQLGQLILCFYITCALFVVLVLGS
IAKATGFSIFRFIAYIKEELLIVLGTSSSESALPRMLDKMEKVGCQKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQAT
NAQMDIWHQVTLLVVLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLIGNGVATIVVAK
RCRQLNEKQMNAVLGGRDSALRDPIA
>Mature_426_residues
MKKSIFTSLYLQVLVAITIGILLGHFYPDLGTQMKPLGDGFVKLIKMIIAPVIFCTVVTGIAGMESMKAVGRTGAIALLY
FEVVSTIALIIGLVVVNILQPGVGMNVDPNALDTKAVAVYADQAAQQGIVAFLLDVIPSSVIGAFASGNILQVLLFAVMF
GFALHHLGEKGQLIFNVIDSFAKVIFGVINMIMKLAPIGAFGAMAFTIGKYGVGTLVQLGQLILCFYITCALFVVLVLGS
IAKATGFSIFRFIAYIKEELLIVLGTSSSESALPRMLDKMEKVGCQKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQAT
NAQMDIWHQVTLLVVLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLIGNGVATIVVAK
RCRQLNEKQMNAVLGGRDSALRDPIA

Specific function: Responsible for the transport of dicarboxylates such as succinate, fumarate, and malate from the periplasm across the membrane

COG id: COG1301

COG function: function code C; Na+/H+-dicarboxylate symporters

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:dicarboxylate (SDF) symporter (TC 2.A.23) family

Homologues:

Organism=Homo sapiens, GI5032093, Length=379, Percent_Identity=31.1345646437995, Blast_Score=127, Evalue=2e-29,
Organism=Homo sapiens, GI169790839, Length=427, Percent_Identity=25.9953161592506, Blast_Score=122, Evalue=4e-28,
Organism=Homo sapiens, GI223468564, Length=249, Percent_Identity=35.3413654618474, Blast_Score=120, Evalue=2e-27,
Organism=Homo sapiens, GI223468566, Length=249, Percent_Identity=35.3413654618474, Blast_Score=120, Evalue=2e-27,
Organism=Homo sapiens, GI21314632, Length=421, Percent_Identity=27.3159144893112, Blast_Score=120, Evalue=2e-27,
Organism=Homo sapiens, GI40254478, Length=430, Percent_Identity=25.8139534883721, Blast_Score=119, Evalue=4e-27,
Organism=Homo sapiens, GI4827012, Length=394, Percent_Identity=26.1421319796954, Blast_Score=118, Evalue=1e-26,
Organism=Homo sapiens, GI66773030, Length=469, Percent_Identity=26.226012793177, Blast_Score=112, Evalue=7e-25,
Organism=Homo sapiens, GI194239697, Length=473, Percent_Identity=23.6786469344609, Blast_Score=110, Evalue=2e-24,
Organism=Homo sapiens, GI262359914, Length=375, Percent_Identity=24.8, Blast_Score=89, Evalue=9e-18,
Organism=Homo sapiens, GI301601644, Length=177, Percent_Identity=31.638418079096, Blast_Score=80, Evalue=2e-15,
Organism=Escherichia coli, GI1789947, Length=417, Percent_Identity=86.5707434052758, Blast_Score=729, Evalue=0.0,
Organism=Escherichia coli, GI1790514, Length=434, Percent_Identity=38.9400921658986, Blast_Score=313, Evalue=2e-86,
Organism=Escherichia coli, GI1788024, Length=401, Percent_Identity=21.9451371571072, Blast_Score=85, Evalue=1e-17,
Organism=Escherichia coli, GI1789473, Length=241, Percent_Identity=25.7261410788382, Blast_Score=74, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17537407, Length=436, Percent_Identity=25.2293577981651, Blast_Score=139, Evalue=4e-33,
Organism=Caenorhabditis elegans, GI71996953, Length=423, Percent_Identity=25.531914893617, Blast_Score=117, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI71983106, Length=410, Percent_Identity=24.8780487804878, Blast_Score=115, Evalue=3e-26,
Organism=Caenorhabditis elegans, GI71983099, Length=410, Percent_Identity=24.8780487804878, Blast_Score=115, Evalue=4e-26,
Organism=Caenorhabditis elegans, GI193206654, Length=427, Percent_Identity=23.6533957845433, Blast_Score=107, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI193206505, Length=467, Percent_Identity=23.1263383297645, Blast_Score=107, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI17541374, Length=402, Percent_Identity=24.1293532338308, Blast_Score=101, Evalue=9e-22,
Organism=Drosophila melanogaster, GI24583025, Length=396, Percent_Identity=24.2424242424242, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI17137668, Length=396, Percent_Identity=24.2424242424242, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI24583023, Length=396, Percent_Identity=24.2424242424242, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI281360481, Length=466, Percent_Identity=25.1072961373391, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI281360483, Length=466, Percent_Identity=25.1072961373391, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI17137666, Length=466, Percent_Identity=25.1072961373391, Blast_Score=94, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DCTA_EDWI9 (C5BB35)

Other databases:

- EMBL:   CP001600
- RefSeq:   YP_002935112.1
- GeneID:   7960763
- GenomeReviews:   CP001600_GR
- KEGG:   eic:NT01EI_3750
- OMA:   HVIFRIV
- ProtClustDB:   PRK01663
- HAMAP:   MF_01300
- InterPro:   IPR001991
- InterPro:   IPR018107
- PANTHER:   PTHR11958
- PRINTS:   PR00173

Pfam domain/function: PF00375 SDF

EC number: NA

Molecular weight: Translated: 44938; Mature: 44938

Theoretical pI: Translated: 9.11; Mature: 9.11

Prosite motif: PS00713 NA_DICARBOXYL_SYMP_1; PS00714 NA_DICARBOXYL_SYMP_2

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x2083f058)-; HASH(0x20072c6c)-; HASH(0x20a37f50)-; HASH(0x20a20b78)-; HASH(0x20953fa4)-; HASH(0x20a23dcc)-; HASH(0x20a26ed8)-; HASH(0x20a35538)-;

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HCCCCH
>Mature Secondary Structure
MKKSIFTSLYLQVLVAITIGILLGHFYPDLGTQMKPLGDGFVKLIKMIIAPVIFCTVVTG
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
IAGMESMKAVGRTGAIALLYFEVVSTIALIIGLVVVNILQPGVGMNVDPNALDTKAVAVY
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHH
ADQAAQQGIVAFLLDVIPSSVIGAFASGNILQVLLFAVMFGFALHHLGEKGQLIFNVIDS
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
FAKVIFGVINMIMKLAPIGAFGAMAFTIGKYGVGTLVQLGQLILCFYITCALFVVLVLGS
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IAKATGFSIFRFIAYIKEELLIVLGTSSSESALPRMLDKMEKVGCQKSVVGLVIPTGYSF
HHHHHHHHHHHHHHHHHHHHEEEEECCCCHHHHHHHHHHHHHCCCHHHHEEEEEECCEEE
NLDGTSIYLTMAAVFIAQATNAQMDIWHQVTLLVVLLLSSKGAAGVTGSGFIVLAATLSA
CCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHH
VGHLPVAGLALILGIDRFMSEARALTNLIGNGVATIVVAKRCRQLNEKQMNAVLGGRDSA
HCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
LRDPIA
HCCCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: orotate [Periplasm]; fumarate [Periplasm]; malate [Periplasm]; Na (I) [Periplasm]; succinate [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + orotate [Periplasm] = Na (I) [Cytoplasm] + orotate [Cytoplasm] Na (I) [Periplasm] + fumarate [Periplasm] = Na (I) [Cytoplasm] + fumarate [Cytoplasm] Na (I) [Periplasm] + malate [Periplasm] = Na (I) [Cytoplasm] + malate [Cytoplasm] Na

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA