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The map label for this gene is mrcA [H]

Identifier: 238921478

GI number: 238921478

Start: 3515146

End: 3517710

Strand: Direct

Name: mrcA [H]

Synonym: NT01EI_3630

Alternate gene names: 238921478

Gene position: 3515146-3517710 (Clockwise)

Preceding gene: 238921465

Following gene: 238921481

Centisome position: 92.21

GC content: 58.91

Gene sequence:

>2565_bases
GTGAAGTTCGTAAAGTATTTAATCATCCTCGCGGTCTGTTGCATTCTGCTGGGAGTCGCTGCGGTATTTGGCCTGTACAA
ATACGTTGAGCCGCAGCTCCCGGACGTTGCAACGCTGAAAGACGTGCGGCTACAGACACCGATGCAGGTGTACAGCGCCG
ACGGCTATCTCATCGCCCAATATGGTGAAAAGCGCCGTATTCCGCTGCCGCTCACGCAGATCCCCCCGCTGATGGTCAAG
GCGTTTATCGCTACCGAAGACAGCCGTTTCTTCGAACACCACGGCGTTGATCCTATCGGCATCTTCCGTGCCGCCTCCGT
CGCCCTGTTTACCGGGCATGCCTCGCAGGGCGCCAGTACCATCACTCAGCAGTTGGCCAGGAACTTTTTCCTCAGCCCAG
AGCGAACGCTGATGCGTAAAATCAAGGAGGCCTTTCTCGCCATTCGCATCGAGCAGCTATTGAATAAAGACGAAATTCTC
GATCTTTACCTCAATAAAATTTATCTGGGCTATCGCGCCTACGGCGTCGGCGCTGCAGCGCAGGTCTACTTTGGCAAATC
CGTCGATCAGCTCACGCTGAGTGAAATGGCCATGATCGCGGGCCTGCCCAAAGCACCGTCCACCTTTAATCCACTCTACT
CCCACGATCGTGCTGTCGCCCGCCGCAACGTCGTGCTTAGCCGGATGCTGGATGAACACTACATCACCCAGGCACAGTAT
GATCAGGCACGCAGCGAACCGCTGGTCTCCCGCTACCACGGGCCTGAGATCGACTTCTCCGCCCCCTATCTGGCCGAAAT
GGTGCGCCAGCAGATGGTGGAGAAATACGGTGAAAATGCCTATACCGACGGCTTTAAGGTCTACACCACGATCACCAAAC
ACTTACAGGTTGCCGCCCAAACGGCACTGCGTAATAACGTGATCAATTACGATATGCGCCATGGCTACCGCGGCCCCTCC
AACGTACTGTGGAAAGTCGGGCAGTCTGCCTGGAGCCAGCAGCGGATCACCGATACGCTTAACGGTCTACCCTCCTACGG
CCCGCTACTGCCAGCTGTTGTGATGTCGGCCGATGCCGACAGCGCTACGGCCATGTTAGCCAACGGCAACAGCGTTGCGC
TGCCGCTGACGACCGTGCGCTGGGCCCGCCCTTACCGCAGCGATCGCGTGCAGGGTGCCACACCGCGTAAAGTCAGCGAT
GTCCTGCAGCCGGGACAACAGATTTGGGTGCGTCAGGATGGCGATAAGCACTGGTGGTTGGCACAGGTGCCGGACGTCAA
CTCCGCCCTGGTCTCCATCAGCCCGCAGGATGGTGCCATCAAGGCACTGGTCGGTGGTTTTGACTTTAATCAGAGTAAAT
TTAACCGGGTCACTCAATCGGTGCGCCAGGTCGGATCCAATATCAAACCGTTCCTGTACACCGCCGCCATGGATAAGGGG
CTGACGCTGGCGACCATCCTAAACGATCTGCCCATCTCCCGTTGGGATGCCGGTGCAGGTACCGATTGGCGGCCCAAAAA
CTCACCGCCAACCTATGCCGGTCCCATTCGCCTGCGTCAGGGTCTCGGTGAGTCGAAAAACGTGGTAATGGTACGTGCCA
TGCGCGCCATGGGTGTCGATTACGCCGCAGACTATCTGCTGCGCTTCGGCTTCCCCGCACAGAACATCGTGAAGACCGAA
TCCCTGGCGCTGGGCTCAGCCAACTTTACCCCACTACAGCTGGTACGCGGCTACGCCGTCTTTGCCAACGGCGGCTATCT
GGTCGACCCCTACTTCATCACCAAGGTCGTCGACGGCAGCGATCAGACGATCTATGAAGCTCATCCGAAGGTCGTATGCG
ACAGCTGTAATCTACCGGTTATCTACGGCCCGACGCCGAAGTCAGCGGTATTGAGCGACGACAGTGTAGAAAACGTCGCT
GTCTCAACGACCCCGAGCGTGAACGGCAACGTGCCGATGCCGGAGCTGGAGCAGGTCACGCCAAGTCAGGTCAGTCAGGA
TAGCGATCAGCGCTATGCGCCGCACGTTATCAGCACGCCGCTGGCCTTCCTGATGCACGATGCGCTGAGCAGCAACATCT
GGGGAGAACCGGGCTGGATGGGAACCGGGTGGCGCGCGATGCGCGATCTGAAGCGCCACGATATCGGCGGGAAAACCGGC
ACGACCAACAGCTCCAAAGACGCCTGGTTCTCCGGTTACGGTCCGGGTATTGCTACCAGCGTCTGGATAGGCTTTGACGA
TCATCGTCGCGATCTGGGACGCACCACCGTATCTGGGGCCCTGAAGGATCAGATTTCCGGCGGTGAGGGCGGAGCCAAGA
GCGCCCAGCCGGCATGGGACGACTTTATGAAGTCGGCGCTGACCGGAATCCCCGAGCAGCCGCTGCCGCCGCCACCGGAC
ATTGTCAGCGTCGTTATCGACAGGGCGACCGGCAAGCTGGCCACCGGCAGCGGTAACAGCCGTAGCGAGTACTTCATTGA
GGGGACCCAGCCGACGGACTATGCTACGCCGGATGCCGGCACCGTGATCATCAACAATGAGGGGCAGAGTCAGGAGCTAT
TCTGA

Upstream 100 bases:

>100_bases
TCAACTTACTAAAAGAACGTATCCTCTGTGTCTGCTAGCCTTTATACTACTGCCCGTTTGTTTATAAACTGCCCAACTGA
CATGTAATGGGAAATTCCAG

Downstream 100 bases:

>100_bases
TAGCCCCCCTCATTTTCACTAATGGGAAAGAGCCGTCAGACCTGACGGCTCTCCATAAATAAAGACAGGCATAGAGAGTT
GTTGTTGTCTACTGAGTATT

Product: peptidoglycan synthetase

Products: NA

Alternate protein names: PBP-1a; PBP1a; Penicillin-insensitive transglycosylase; Peptidoglycan TGase; Penicillin-sensitive transpeptidase; DD-transpeptidase [H]

Number of amino acids: Translated: 854; Mature: 854

Protein sequence:

>854_residues
MKFVKYLIILAVCCILLGVAAVFGLYKYVEPQLPDVATLKDVRLQTPMQVYSADGYLIAQYGEKRRIPLPLTQIPPLMVK
AFIATEDSRFFEHHGVDPIGIFRAASVALFTGHASQGASTITQQLARNFFLSPERTLMRKIKEAFLAIRIEQLLNKDEIL
DLYLNKIYLGYRAYGVGAAAQVYFGKSVDQLTLSEMAMIAGLPKAPSTFNPLYSHDRAVARRNVVLSRMLDEHYITQAQY
DQARSEPLVSRYHGPEIDFSAPYLAEMVRQQMVEKYGENAYTDGFKVYTTITKHLQVAAQTALRNNVINYDMRHGYRGPS
NVLWKVGQSAWSQQRITDTLNGLPSYGPLLPAVVMSADADSATAMLANGNSVALPLTTVRWARPYRSDRVQGATPRKVSD
VLQPGQQIWVRQDGDKHWWLAQVPDVNSALVSISPQDGAIKALVGGFDFNQSKFNRVTQSVRQVGSNIKPFLYTAAMDKG
LTLATILNDLPISRWDAGAGTDWRPKNSPPTYAGPIRLRQGLGESKNVVMVRAMRAMGVDYAADYLLRFGFPAQNIVKTE
SLALGSANFTPLQLVRGYAVFANGGYLVDPYFITKVVDGSDQTIYEAHPKVVCDSCNLPVIYGPTPKSAVLSDDSVENVA
VSTTPSVNGNVPMPELEQVTPSQVSQDSDQRYAPHVISTPLAFLMHDALSSNIWGEPGWMGTGWRAMRDLKRHDIGGKTG
TTNSSKDAWFSGYGPGIATSVWIGFDDHRRDLGRTTVSGALKDQISGGEGGAKSAQPAWDDFMKSALTGIPEQPLPPPPD
IVSVVIDRATGKLATGSGNSRSEYFIEGTQPTDYATPDAGTVIINNEGQSQELF

Sequences:

>Translated_854_residues
MKFVKYLIILAVCCILLGVAAVFGLYKYVEPQLPDVATLKDVRLQTPMQVYSADGYLIAQYGEKRRIPLPLTQIPPLMVK
AFIATEDSRFFEHHGVDPIGIFRAASVALFTGHASQGASTITQQLARNFFLSPERTLMRKIKEAFLAIRIEQLLNKDEIL
DLYLNKIYLGYRAYGVGAAAQVYFGKSVDQLTLSEMAMIAGLPKAPSTFNPLYSHDRAVARRNVVLSRMLDEHYITQAQY
DQARSEPLVSRYHGPEIDFSAPYLAEMVRQQMVEKYGENAYTDGFKVYTTITKHLQVAAQTALRNNVINYDMRHGYRGPS
NVLWKVGQSAWSQQRITDTLNGLPSYGPLLPAVVMSADADSATAMLANGNSVALPLTTVRWARPYRSDRVQGATPRKVSD
VLQPGQQIWVRQDGDKHWWLAQVPDVNSALVSISPQDGAIKALVGGFDFNQSKFNRVTQSVRQVGSNIKPFLYTAAMDKG
LTLATILNDLPISRWDAGAGTDWRPKNSPPTYAGPIRLRQGLGESKNVVMVRAMRAMGVDYAADYLLRFGFPAQNIVKTE
SLALGSANFTPLQLVRGYAVFANGGYLVDPYFITKVVDGSDQTIYEAHPKVVCDSCNLPVIYGPTPKSAVLSDDSVENVA
VSTTPSVNGNVPMPELEQVTPSQVSQDSDQRYAPHVISTPLAFLMHDALSSNIWGEPGWMGTGWRAMRDLKRHDIGGKTG
TTNSSKDAWFSGYGPGIATSVWIGFDDHRRDLGRTTVSGALKDQISGGEGGAKSAQPAWDDFMKSALTGIPEQPLPPPPD
IVSVVIDRATGKLATGSGNSRSEYFIEGTQPTDYATPDAGTVIINNEGQSQELF
>Mature_854_residues
MKFVKYLIILAVCCILLGVAAVFGLYKYVEPQLPDVATLKDVRLQTPMQVYSADGYLIAQYGEKRRIPLPLTQIPPLMVK
AFIATEDSRFFEHHGVDPIGIFRAASVALFTGHASQGASTITQQLARNFFLSPERTLMRKIKEAFLAIRIEQLLNKDEIL
DLYLNKIYLGYRAYGVGAAAQVYFGKSVDQLTLSEMAMIAGLPKAPSTFNPLYSHDRAVARRNVVLSRMLDEHYITQAQY
DQARSEPLVSRYHGPEIDFSAPYLAEMVRQQMVEKYGENAYTDGFKVYTTITKHLQVAAQTALRNNVINYDMRHGYRGPS
NVLWKVGQSAWSQQRITDTLNGLPSYGPLLPAVVMSADADSATAMLANGNSVALPLTTVRWARPYRSDRVQGATPRKVSD
VLQPGQQIWVRQDGDKHWWLAQVPDVNSALVSISPQDGAIKALVGGFDFNQSKFNRVTQSVRQVGSNIKPFLYTAAMDKG
LTLATILNDLPISRWDAGAGTDWRPKNSPPTYAGPIRLRQGLGESKNVVMVRAMRAMGVDYAADYLLRFGFPAQNIVKTE
SLALGSANFTPLQLVRGYAVFANGGYLVDPYFITKVVDGSDQTIYEAHPKVVCDSCNLPVIYGPTPKSAVLSDDSVENVA
VSTTPSVNGNVPMPELEQVTPSQVSQDSDQRYAPHVISTPLAFLMHDALSSNIWGEPGWMGTGWRAMRDLKRHDIGGKTG
TTNSSKDAWFSGYGPGIATSVWIGFDDHRRDLGRTTVSGALKDQISGGEGGAKSAQPAWDDFMKSALTGIPEQPLPPPPD
IVSVVIDRATGKLATGSGNSRSEYFIEGTQPTDYATPDAGTVIINNEGQSQELF

Specific function: Cell wall formation. Synthesis of cross-linked peptidoglycan from the lipid intermediates. The enzyme has a penicillin-insensitive transglycosylase N-terminal domain (formation of linear glycan strands) and a penicillin-sensitive transpeptidase C-terminal

COG id: COG5009

COG function: function code M; Membrane carboxypeptidase/penicillin-binding protein

Gene ontology:

Cell location: Cell inner membrane; Single-pass type II membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI87082258, Length=854, Percent_Identity=76.5807962529274, Blast_Score=1371, Evalue=0.0,
Organism=Escherichia coli, GI1786343, Length=251, Percent_Identity=36.2549800796813, Blast_Score=147, Evalue=2e-36,
Organism=Escherichia coli, GI1788867, Length=293, Percent_Identity=35.1535836177474, Blast_Score=130, Evalue=4e-31,
Organism=Escherichia coli, GI1789601, Length=176, Percent_Identity=37.5, Blast_Score=95, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR001264
- InterPro:   IPR011816
- InterPro:   IPR001460 [H]

Pfam domain/function: PF00912 Transgly; PF00905 Transpeptidase [H]

EC number: 2.4.2.-; 3.4.-.-

Molecular weight: Translated: 93700; Mature: 93700

Theoretical pI: Translated: 7.98; Mature: 7.98

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFVKYLIILAVCCILLGVAAVFGLYKYVEPQLPDVATLKDVRLQTPMQVYSADGYLIAQ
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCHHEECCCCEEEEE
YGEKRRIPLPLTQIPPLMVKAFIATEDSRFFEHHGVDPIGIFRAASVALFTGHASQGAST
CCCCCCCCCCHHHCCHHHHHHHHHCCCHHHHHHCCCCHHHHHHHHHEEEEECCCCCCHHH
ITQQLARNFFLSPERTLMRKIKEAFLAIRIEQLLNKDEILDLYLNKIYLGYRAYGVGAAA
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHEECCCCCE
QVYFGKSVDQLTLSEMAMIAGLPKAPSTFNPLYSHDRAVARRNVVLSRMLDEHYITQAQY
EEEECCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DQARSEPLVSRYHGPEIDFSAPYLAEMVRQQMVEKYGENAYTDGFKVYTTITKHLQVAAQ
HHHHCCCHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEHHHHHHHHHHHHHH
TALRNNVINYDMRHGYRGPSNVLWKVGQSAWSQQRITDTLNGLPSYGPLLPAVVMSADAD
HHHHHCCCCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCC
SATAMLANGNSVALPLTTVRWARPYRSDRVQGATPRKVSDVLQPGQQIWVRQDGDKHWWL
CCEEEEECCCEEEEEEHHHHHCCCCCCCCCCCCCCHHHHHHHCCCCEEEEEECCCCEEEE
AQVPDVNSALVSISPQDGAIKALVGGFDFNQSKFNRVTQSVRQVGSNIKPFLYTAAMDKG
EECCCCCCEEEEECCCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEHHCCC
LTLATILNDLPISRWDAGAGTDWRPKNSPPTYAGPIRLRQGLGESKNVVMVRAMRAMGVD
CCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCEEEEEEHHHHCCH
YAADYLLRFGFPAQNIVKTESLALGSANFTPLQLVRGYAVFANGGYLVDPYFITKVVDGS
HHHHHHHHCCCCHHHHHHHHHEEECCCCCCHHHHHCCEEEEECCCEEECCCEEEEEECCC
DQTIYEAHPKVVCDSCNLPVIYGPTPKSAVLSDDSVENVAVSTTPSVNGNVPMPELEQVT
CCEEEECCCCEEECCCCCCEEECCCCCCCEECCCCCCCEEEECCCCCCCCCCCCHHHHCC
PSQVSQDSDQRYAPHVISTPLAFLMHDALSSNIWGEPGWMGTGWRAMRDLKRHDIGGKTG
HHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCCCCC
TTNSSKDAWFSGYGPGIATSVWIGFDDHRRDLGRTTVSGALKDQISGGEGGAKSAQPAWD
CCCCCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHH
DFMKSALTGIPEQPLPPPPDIVSVVIDRATGKLATGSGNSRSEYFIEGTQPTDYATPDAG
HHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCEECCCCCCCCCEEEECCCCCCCCCCCCC
TVIINNEGQSQELF
EEEECCCCCCCCCC
>Mature Secondary Structure
MKFVKYLIILAVCCILLGVAAVFGLYKYVEPQLPDVATLKDVRLQTPMQVYSADGYLIAQ
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCHHEECCCCEEEEE
YGEKRRIPLPLTQIPPLMVKAFIATEDSRFFEHHGVDPIGIFRAASVALFTGHASQGAST
CCCCCCCCCCHHHCCHHHHHHHHHCCCHHHHHHCCCCHHHHHHHHHEEEEECCCCCCHHH
ITQQLARNFFLSPERTLMRKIKEAFLAIRIEQLLNKDEILDLYLNKIYLGYRAYGVGAAA
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHEECCCCCE
QVYFGKSVDQLTLSEMAMIAGLPKAPSTFNPLYSHDRAVARRNVVLSRMLDEHYITQAQY
EEEECCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DQARSEPLVSRYHGPEIDFSAPYLAEMVRQQMVEKYGENAYTDGFKVYTTITKHLQVAAQ
HHHHCCCHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEHHHHHHHHHHHHHH
TALRNNVINYDMRHGYRGPSNVLWKVGQSAWSQQRITDTLNGLPSYGPLLPAVVMSADAD
HHHHHCCCCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCC
SATAMLANGNSVALPLTTVRWARPYRSDRVQGATPRKVSDVLQPGQQIWVRQDGDKHWWL
CCEEEEECCCEEEEEEHHHHHCCCCCCCCCCCCCCHHHHHHHCCCCEEEEEECCCCEEEE
AQVPDVNSALVSISPQDGAIKALVGGFDFNQSKFNRVTQSVRQVGSNIKPFLYTAAMDKG
EECCCCCCEEEEECCCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEHHCCC
LTLATILNDLPISRWDAGAGTDWRPKNSPPTYAGPIRLRQGLGESKNVVMVRAMRAMGVD
CCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCEEEEEEHHHHCCH
YAADYLLRFGFPAQNIVKTESLALGSANFTPLQLVRGYAVFANGGYLVDPYFITKVVDGS
HHHHHHHHCCCCHHHHHHHHHEEECCCCCCHHHHHCCEEEEECCCEEECCCEEEEEECCC
DQTIYEAHPKVVCDSCNLPVIYGPTPKSAVLSDDSVENVAVSTTPSVNGNVPMPELEQVT
CCEEEECCCCEEECCCCCCEEECCCCCCCEECCCCCCCEEEECCCCCCCCCCCCHHHHCC
PSQVSQDSDQRYAPHVISTPLAFLMHDALSSNIWGEPGWMGTGWRAMRDLKRHDIGGKTG
HHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCCCCC
TTNSSKDAWFSGYGPGIATSVWIGFDDHRRDLGRTTVSGALKDQISGGEGGAKSAQPAWD
CCCCCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHH
DFMKSALTGIPEQPLPPPPDIVSVVIDRATGKLATGSGNSRSEYFIEGTQPTDYATPDAG
HHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCEECCCCCCCCCEEEECCCCCCCCCCCCC
TVIINNEGQSQELF
EEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Acyltransferases; Aminoacyltransferases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 3882429; 9278503; 3920658; 7006606; 9841666 [H]