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The map label for this gene is cysG

Identifier: 238921465

GI number: 238921465

Start: 3503827

End: 3505212

Strand: Direct

Name: cysG

Synonym: NT01EI_3617

Alternate gene names: 238921465

Gene position: 3503827-3505212 (Clockwise)

Preceding gene: 238921462

Following gene: 238921478

Centisome position: 91.91

GC content: 63.42

Gene sequence:

>1386_bases
ATGGATTACTTTCCGATATTTTGTCAGCTGCGTACTAAGCCCTGCCTGCTGGTCGGCGGCGGCGAGGTGGCCGAGCGTAA
AGCCCGTCTGTTGATGGAGGCGGGCGCGATTTTGACGGTGAACGCCGGGCGCTTCACGCCCCAGTTTGAACAGTGGCAAC
GGGACGGTCAGCTGACGCTGATCGCCGGCGACTTCGACCCCGCGCTGCTCACGGGTAAGTGGCTGGCTATCGCCGCCACC
GATGACAGCCGGGTAAATCAGCAGGTGCTGGCCCAGTCCGAGGCGCGCTGTATCTTTTGTAACGTGGTCGACGCCGCGCA
ACAGACGGGCTTTATCATGCCGTCGATCGTCGATCGCTCGCCGTTGATGGTCGCCGTCTCCTCCGGTGGCAGCGCACCGG
TGCTGGCCAGAATTCTGCGCGAAAAGCTGGAGGCGCTGCTACCACAGCATCTCGGCCAGGTGGCACACATGGCTGGCCAC
CTGCGCCAGCGCGTCAAGGCACACTTTCCCACCCTGTCGCTGCGCCGCCGCTTTTGGGAACGGCTGTTCGCCCAAGATCG
TCTGGCACAGTCGCTGGCCAACGGCGATACCACGCAGGCACAGCAACAGGTGGATGCGCTATTCAGCGCGGCGCCGATGG
ATCGCGGCGAGGTGACGCTGGTCGGCGCCGGGCCAGGGGATGCAGGGCTGTTGACCCTCAAAGGGCTACAGCAGATCCAA
CAGGCAGATGTGATCATCTATGACCGCCTGGTATCCGATGAGGTCATGTCACTGGTGCGGCGTGACGCGACGCGTATCTT
TGTCGGCAAACACGCCGGTCATCACTGCGTTCCCCAGGAAGAGATTAATCAGACTCTGCTGGATCACGCGCGTGAGGGCA
AACGGGTAGTGCGCCTAAAGGGCGGCGATCCCTTCATCTTTGGCCGGGGAGGCGAAGAGCTTGAGGCGCTGGCCGCTGCG
GGGATCGCCTTTTCGGTGGTGCCCGGTATTACCGCCGCCTCCGGCTGCTCCGCCTATAGCGGTATTCCGCTGACTCACCG
CGACCATGCCCAAAGTGTCCGTCTGGTCACCGGCCACACCCGCCAGGATGGCCAGCTGGACTGGTCCTGCCTGGCGGCCG
CAGGACAAACGCTCGTGTTTTATATGGGACTGAGCCAGGCGGCAACCATTCAGCAGCGCCTCCTGCAGCACGGCATGCTG
CCAGATACCCCCATCGCCCTGGTTGAAAACGGCACCACGATTCGCCAGCGGGTCGTCAGTGGAACGCTGACTCAGCTGGA
GACGCTGGCAACGCGCGTTGCCAGCCCCAGTCTGATCATCGTCGGCGACGTCGTCACCCTGCGGCCACGCCTCAACTGGT
TCCGCTGCGAAGCGGCAAGCGCATAA

Upstream 100 bases:

>100_bases
CAACGCGTCTGGCAAGCTGGAGAGTCTCTGCCCCTTGGCGTACTATCCCCGCTCGCGGAATATGGCCGCCCCACAACAGG
CTCACCCTTGAGGATCTCTG

Downstream 100 bases:

>100_bases
CACAGCGCCACAGCACAAAAAAAGGAGGGCCATCGCCCTCCCCTTCACGTTCAGATCGCCGCGCTGCTTACGGCCGCGCG
ACAAACCCGATCGCATCGTA

Product: siroheme synthase

Products: NA

Alternate protein names: Uroporphyrinogen-III C-methyltransferase; Urogen III methylase; SUMT; Uroporphyrinogen III methylase; UROM; Precorrin-2 dehydrogenase; Sirohydrochlorin ferrochelatase

Number of amino acids: Translated: 461; Mature: 461

Protein sequence:

>461_residues
MDYFPIFCQLRTKPCLLVGGGEVAERKARLLMEAGAILTVNAGRFTPQFEQWQRDGQLTLIAGDFDPALLTGKWLAIAAT
DDSRVNQQVLAQSEARCIFCNVVDAAQQTGFIMPSIVDRSPLMVAVSSGGSAPVLARILREKLEALLPQHLGQVAHMAGH
LRQRVKAHFPTLSLRRRFWERLFAQDRLAQSLANGDTTQAQQQVDALFSAAPMDRGEVTLVGAGPGDAGLLTLKGLQQIQ
QADVIIYDRLVSDEVMSLVRRDATRIFVGKHAGHHCVPQEEINQTLLDHAREGKRVVRLKGGDPFIFGRGGEELEALAAA
GIAFSVVPGITAASGCSAYSGIPLTHRDHAQSVRLVTGHTRQDGQLDWSCLAAAGQTLVFYMGLSQAATIQQRLLQHGML
PDTPIALVENGTTIRQRVVSGTLTQLETLATRVASPSLIIVGDVVTLRPRLNWFRCEAASA

Sequences:

>Translated_461_residues
MDYFPIFCQLRTKPCLLVGGGEVAERKARLLMEAGAILTVNAGRFTPQFEQWQRDGQLTLIAGDFDPALLTGKWLAIAAT
DDSRVNQQVLAQSEARCIFCNVVDAAQQTGFIMPSIVDRSPLMVAVSSGGSAPVLARILREKLEALLPQHLGQVAHMAGH
LRQRVKAHFPTLSLRRRFWERLFAQDRLAQSLANGDTTQAQQQVDALFSAAPMDRGEVTLVGAGPGDAGLLTLKGLQQIQ
QADVIIYDRLVSDEVMSLVRRDATRIFVGKHAGHHCVPQEEINQTLLDHAREGKRVVRLKGGDPFIFGRGGEELEALAAA
GIAFSVVPGITAASGCSAYSGIPLTHRDHAQSVRLVTGHTRQDGQLDWSCLAAAGQTLVFYMGLSQAATIQQRLLQHGML
PDTPIALVENGTTIRQRVVSGTLTQLETLATRVASPSLIIVGDVVTLRPRLNWFRCEAASA
>Mature_461_residues
MDYFPIFCQLRTKPCLLVGGGEVAERKARLLMEAGAILTVNAGRFTPQFEQWQRDGQLTLIAGDFDPALLTGKWLAIAAT
DDSRVNQQVLAQSEARCIFCNVVDAAQQTGFIMPSIVDRSPLMVAVSSGGSAPVLARILREKLEALLPQHLGQVAHMAGH
LRQRVKAHFPTLSLRRRFWERLFAQDRLAQSLANGDTTQAQQQVDALFSAAPMDRGEVTLVGAGPGDAGLLTLKGLQQIQ
QADVIIYDRLVSDEVMSLVRRDATRIFVGKHAGHHCVPQEEINQTLLDHAREGKRVVRLKGGDPFIFGRGGEELEALAAA
GIAFSVVPGITAASGCSAYSGIPLTHRDHAQSVRLVTGHTRQDGQLDWSCLAAAGQTLVFYMGLSQAATIQQRLLQHGML
PDTPIALVENGTTIRQRVVSGTLTQLETLATRVASPSLIIVGDVVTLRPRLNWFRCEAASA

Specific function: Multifunctional enzyme that catalyzes the SAM-dependent methylation of uroporphyrinogen III at position C-2 and C-7 to form precorrin-2 and then position C-12 or C-18 to form trimethylpyrrocorphin 2. It also catalyzes the conversion of precorrin-2 into si

COG id: COG0007

COG function: function code H; Uroporphyrinogen-III methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the precorrin methyltransferase family

Homologues:

Organism=Escherichia coli, GI1789768, Length=455, Percent_Identity=70.7692307692308, Blast_Score=652, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6322922, Length=259, Percent_Identity=34.3629343629344, Blast_Score=144, Evalue=4e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CYSG_EDWI9 (C5BGP8)

Other databases:

- EMBL:   CP001600
- RefSeq:   YP_002934980.1
- ProteinModelPortal:   C5BGP8
- GeneID:   7961438
- GenomeReviews:   CP001600_GR
- KEGG:   eic:NT01EI_3617
- OMA:   MCRRDAE
- ProtClustDB:   PRK10637
- GO:   GO:0005488
- HAMAP:   MF_01646
- InterPro:   IPR000878
- InterPro:   IPR014777
- InterPro:   IPR014776
- InterPro:   IPR006366
- InterPro:   IPR016040
- InterPro:   IPR019478
- InterPro:   IPR006367
- InterPro:   IPR003043
- Gene3D:   G3DSA:3.40.1010.10
- Gene3D:   G3DSA:3.30.950.10
- Gene3D:   G3DSA:3.40.50.720
- Gene3D:   G3DSA:1.10.8.210
- TIGRFAMs:   TIGR01469
- TIGRFAMs:   TIGR01470

Pfam domain/function: PF10414 CysG_dimeriser; PF00590 TP_methylase; SSF53790 Cor/por_Metransf

EC number: =2.1.1.107; =1.3.1.76; =4.99.1.4

Molecular weight: Translated: 49999; Mature: 49999

Theoretical pI: Translated: 8.04; Mature: 8.04

Prosite motif: PS00839 SUMT_1; PS00840 SUMT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDYFPIFCQLRTKPCLLVGGGEVAERKARLLMEAGAILTVNAGRFTPQFEQWQRDGQLTL
CCCCEEEEEECCCCEEEECCCHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHCCCCEEE
IAGDFDPALLTGKWLAIAATDDSRVNQQVLAQSEARCIFCNVVDAAQQTGFIMPSIVDRS
EECCCCCCEECCCEEEEEECCCHHHHHHHHHCCCCCEEEEEEHHHHHHCCCCCCHHCCCC
PLMVAVSSGGSAPVLARILREKLEALLPQHLGQVAHMAGHLRQRVKAHFPTLSLRRRFWE
CEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
RLFAQDRLAQSLANGDTTQAQQQVDALFSAAPMDRGEVTLVGAGPGDAGLLTLKGLQQIQ
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCEEEHHHHHHHH
QADVIIYDRLVSDEVMSLVRRDATRIFVGKHAGHHCVPQEEINQTLLDHAREGKRVVRLK
HCCEEEEHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHHHHHHCCCEEEEEE
GGDPFIFGRGGEELEALAAAGIAFSVVPGITAASGCSAYSGIPLTHRDHAQSVRLVTGHT
CCCEEEECCCCHHHHHHHHHCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCC
RQDGQLDWSCLAAAGQTLVFYMGLSQAATIQQRLLQHGMLPDTPIALVENGTTIRQRVVS
CCCCCCCHHHHHHCCCEEEEEECCCHHHHHHHHHHHCCCCCCCCEEEEECCHHHHHHHHH
GTLTQLETLATRVASPSLIIVGDVVTLRPRLNWFRCEAASA
HHHHHHHHHHHHHCCCCEEEEECEEEECCCCCEEEECCCCC
>Mature Secondary Structure
MDYFPIFCQLRTKPCLLVGGGEVAERKARLLMEAGAILTVNAGRFTPQFEQWQRDGQLTL
CCCCEEEEEECCCCEEEECCCHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHCCCCEEE
IAGDFDPALLTGKWLAIAATDDSRVNQQVLAQSEARCIFCNVVDAAQQTGFIMPSIVDRS
EECCCCCCEECCCEEEEEECCCHHHHHHHHHCCCCCEEEEEEHHHHHHCCCCCCHHCCCC
PLMVAVSSGGSAPVLARILREKLEALLPQHLGQVAHMAGHLRQRVKAHFPTLSLRRRFWE
CEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
RLFAQDRLAQSLANGDTTQAQQQVDALFSAAPMDRGEVTLVGAGPGDAGLLTLKGLQQIQ
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCEEEHHHHHHHH
QADVIIYDRLVSDEVMSLVRRDATRIFVGKHAGHHCVPQEEINQTLLDHAREGKRVVRLK
HCCEEEEHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHHHHHHCCCEEEEEE
GGDPFIFGRGGEELEALAAAGIAFSVVPGITAASGCSAYSGIPLTHRDHAQSVRLVTGHT
CCCEEEECCCCHHHHHHHHHCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCC
RQDGQLDWSCLAAAGQTLVFYMGLSQAATIQQRLLQHGMLPDTPIALVENGTTIRQRVVS
CCCCCCCHHHHHHCCCEEEEEECCCHHHHHHHHHHHCCCCCCCCEEEEECCHHHHHHHHH
GTLTQLETLATRVASPSLIIVGDVVTLRPRLNWFRCEAASA
HHHHHHHHHHHHHCCCCEEEEECEEEECCCCCEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA